yfEyfE)LOCUS pIREShyg3 5752 bp DNA SYN 30-Aug-2007 DEFINITION pIREShyg3 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5752 /organism="pIREShyg3" /mol_type="other DNA" misc_feature 315..602 /label="CAG_enhancer" promoter 236..852 /label="CMV_immearly_promoter" misc_feature 769..789 /label="CMV_fwd_primer" promoter 863..881 /label="T7_promoter" misc_feature 1002..1297 /label="synth_int" misc_feature 1334..1898 /label="IRES" CDS complement(1834..2700) /label="ORF frame 1" /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP WFGGPRRFFNLD*" CDS 1819..2967 /label="hygroB" /gene="hygroB" /note="ORF frame 1" /translation="MLYMCLVEVKKTSRPPEPRGRGFPLKNTMISLPQPVPKMDRSGK PELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDR YVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAM DAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQA LDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFW RPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRC DAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*" gene 1933..2964 /label="hygroB" /gene="hygroB" terminator 3266..3494 /label="SV40_PA_terminator" misc_feature 3464..3483 /label="EBV_rev_primer" misc_feature 3516..3535 /label="EBV_rev_primer" misc_feature complement(3591..3609) /label="M13_reverse_primer" misc_feature complement(3608..3630) /label="M13_pUC_rev_primer" promoter complement(3644..3673) /label="lac_promoter" rep_origin complement(3982..4601) /label="pBR322_origin" CDS complement(4756..5616) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4756..5616) /label="Ampicillin" /gene="Ampicillin" promoter complement(5658..5686) /label="AmpR_promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ctgtacaggc cttaagccgg cgcgctagcg cttcgaaggc gcccgggatc 961 cgatatcgta cgttaacata gataactgat ccagtgtgct ggaattaatt cgctgtctgc 1021 gagggccagc tgttggggtg agtactccct ctcaaaagcg ggcatgactt ctgcgctaag 1081 attgtcagtt tccaaaaacg aggaggattt gatattcacc tggcccgcgg tgatgccttt 1141 gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt ttgttgtcaa gcttgaggtg 1201 tggcaggctt gagatctggc catacacttg agtgacaatg acatccactt tgcctttctc 1261 tccacaggtg tccactccca ggtccaactg caggtcgagc atgcatctag ggcggccgca 1321 ctagaggaat tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg 1381 gaataaggcc ggtgtgtgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc 1441 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc 1501 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa 1561 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct 1621 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca 1681 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca 1741 agcgtagtca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggaatctga 1801 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg 1861 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa 1921 cccgtaccaa agatggatag atccggaaag cctgaactca ccgcgacgtc tgtcgagaag 1981 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa 2041 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc 2101 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg 2161 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc 2221 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag 2281 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc 2341 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg 2401 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc 2461 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac 2521 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg 2581 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc 2641 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat 2701 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa 2761 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc 2821 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc 2881 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc 2941 agcactcgtc cgagggcaaa ggaatagagt agatgccgac cgaacaagag ctgatttcga 3001 gaacgcctca gccagcaact cgcgcgagcc tagcaaggca aatgcgagag aacggcctta 3061 cgcttggtgg cacagttctc gtccacagtt cgctaagctc gctcggctgg gtcgcgggag 3121 ggccggtcgc agtgattcag gcccttctgg attgtgttgg tccccagggc acgattgtca 3181 tgcccacgca ctcgggtgat ctgactgatc ccgcagattg gagatcgccg cccgtgcctg 3241 ccgattgggt gcagatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt 3301 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca 3361 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 3421 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 3481 atcaatgtat cttaacgctc gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt 3541 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 3601 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 3661 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 3721 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 3781 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 3841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 3901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 3961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 4021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 4081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 4141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg 4201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 4261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 4321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 4381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 4441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 4501 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 4561 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 4621 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 4681 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 4741 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 4801 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 4861 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 4921 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 4981 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 5041 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 5101 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 5161 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 5221 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 5281 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 5341 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 5401 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 5461 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 5521 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 5581 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 5641 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 5701 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc //x pIREShyg3 "Times New RomanyfECD;ZCAG_enh"Times New RomanyfECDTCMV_immearly_pro"Times New RomanyfECɆD CMV_fwd_pri"Times New RomanyfED_qT7_pro"Times New RomanyfECD synth_int"Times New RomanyfECD6jIRES"Times New RomanyfED ORF 1"Times New RomanyfECɆD* ORF 1"Times New Roman*yfECD hygroB"Times New RomanyfECD  SV40_PA_term"Times New RomanyfECɆD  EBV_rev_pri"Times New RomanyfED  EBV_rev_pri"Times New RomanyfECDM13_reverse_pri"Times New RomanyfECD.M13_pUC_rev_pri"Times New RomanyfECɆD<Ylac_pro"Times New RomanyfED pBR322_origin"Times New RomanyfECDORF 1"Times New RomanyfECD Ampicillin"Times New RomanyfECɆD6AmpR_pro"Times New Roman yfENruI(208),SpeI(249)"Times New RomanD~uk-k yfESStuI(919),AflII(921),NheI(934),BstBI(943),NarI(949),SmaI(954),BamHI(956),EcoRV(964)"Times New RomanDCKSSa yfEMscI(1219),NotI(1313)"Times New RomanD_'h$q!q0 yfEApaI"Times New RomanDecocxcx\j yfEPmlI"Times New RomansD_hqq yfEXbaI(3256),BclI(3266)"Times New Roman D/8AA_ yfEXhoI"Times New Roman D, yfEFspI"Times New RomanD yfEtest"Times New Roman.DAB yfEtest"Times New Roman D$ + +pU yfEtest"Times New Roman D%909