Sequence of pIREShyg3

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Sequence of pIREShyg3

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Sequence of pIREShyg3 : 5752 BP; 1370 A; 1480 C; 1476 G; 1426 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaaccatgg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

.......... ..... StuI(919),AflII(921) NheI(934) BstBI(943) .....

901 gagctcggat ctgtacaggc cttaagccgg cgcgctagcg cttcgaaggc gcccgggatc 960

901 ctcgagccta gacatgtccg gaattcggcc gcgcgatcgc gaagcttccg cgggccctag 960

.......... .......... .......... .......... .......... ..........

EcoRV(964) .......... .......... .......... .......... ..........

961 cgatatcgta cgttaacata gataactgat ccagtgtgct ggaattaatt cgctgtctgc 1020

961 gctatagcat gcaattgtat ctattgacta ggtcacacga ccttaattaa gcgacagacg 1020

.......... .......... .......... .......... .......... synth_int

.......... .......... .......... .......... .......... ..........

1021 gagggccagc tgttggggtg agtactccct ctcaaaagcg ggcatgactt ctgcgctaag 1080

1021 ctcccggtcg acaaccccac tcatgaggga gagttttcgc ccgtactgaa gacgcgattc 1080

.......... .......... synth_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 attgtcagtt tccaaaaacg aggaggattt gatattcacc tggcccgcgg tgatgccttt 1140

1081 taacagtcaa aggtttttgc tcctcctaaa ctataagtgg accgggcgcc actacggaaa 1140

.......... .......... synth_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt ttgttgtcaa gcttgaggtg 1200

1141 ctcccaccgg cgcaggtaga ccagtctttt ctgttagaaa aacaacagtt cgaactccac 1200

.......... .......... synth_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tggcaggctt gagatctggc catacacttg agtgacaatg acatccactt tgcctttctc 1260

1201 accgtccgaa ctctagaccg gtatgtgaac tcactgttac tgtaggtgaa acggaaagag 1260

.......... .......... synth_int .......... .......... ..........

.......... .......... .......... .......... .......... NotI(1313)

1261 tccacaggtg tccactccca ggtccaactg caggtcgagc atgcatctag ggcggccgca 1320

1261 aggtgtccac aggtgagggt ccaggttgac gtccagctcg tacgtagatc ccgccggcgt 1320

.......... synth_int .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctagaggaat tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg 1380

1321 gatctcctta aggcggggag agggaggggg gggggattgc aatgaccggc ttcggcgaac 1380

.......... .......... .......... IRES .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gaataaggcc ggtgtgtgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc 1440

1381 cttattccgg ccacacacaa acagatatac aataaaaggt ggtataacgg cagaaaaccg 1440

.......... .......... IRES .......... .......... ..........

.......... ApaI(1452) .......... .......... .......... ..........

1441 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc 1500

1441 ttacactccc gggcctttgg accgggacag aagaactgct cgtaaggatc cccagaaagg 1500

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa 1560

1501 ggagagcggt ttccttacgt tccagacaac ttacagcact tccttcgtca aggagacctt 1560

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct 1620

1561 cgaagaactt ctgtttgttg cagacatcgc tgggaaacgt ccgtcgcctt ggggggtgga 1620

.......... .......... IRES .......... .......... ..........

.......... .......... .......... PmlI(1651) .......... ..........

1621 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca 1680

1621 ccgctgtcca cggagacgcc ggttttcggt gcacatattc tatgtggacg tttccgccgt 1680

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca 1740

1681 gttggggtca cggtgcaaca ctcaacctat caacaccttt ctcagtttac cgagaggagt 1740

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agcgtagtca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggaatctga 1800

1741 tcgcatcagt tgttccccga cttcctacgg gtcttccatg gggtaacata cccttagact 1800

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg 1860

1801 agaccccgga gccacgtgta cgaaatgtac acaaatcagc tccaattttt ttgcagatcc 1860

.......... .......... IRES ORF 1 ORF 1 ..........

.......... .......... .......... .......... .......... ..........

1861 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa 1920

1861 ggggggcttg gtgcccctgc accaaaagga aactttttgt gctactattc gaacggtgtt 1920

......... IRES ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cccgtaccaa agatggatag atccggaaag cctgaactca ccgcgacgtc tgtcgagaag 1980

1921 gggcatggtt tctacctatc taggcctttc ggacttgagt ggcgctgcag acagctcttc 1980

.......... ......... ORF 1 ORF 1 hygroB ......... ..........

.......... .......... .......... .......... .......... ..........

1981 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa 2040

1981 aaagactagc ttttcaagct gtcgcagagg ctggactacg tcgagagcct cccgcttctt 2040

.......... ...... ORF 1 ORF 1 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc 2100

2041 agagcacgaa agtcgaagct acatcctccc gcacctatac aggacgccca tttatcgacg 2100

.......... ...... ORF 1 ORF 1 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg 2160

2101 cggctaccaa agatgtttct agcaatacaa atagccgtga aacgtagccg gcgcgagggc 2160

.......... ...... ORF 1 ORF 1 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc 2220

2161 taaggccttc acgaactgta accccttaag tcgctctcgg actggataac gtagagggcg 2220

.......... ...... ORF 1 ORF 1 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag 2280

2221 gcacgtgtcc cacagtgcaa cgttctggac ggactttggc ttgacgggcg acaagacgtc 2280

.......... ...... ORF 1 ORF 1 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc 2340

2281 ggccagcgcc tccggtacct acgctagcga cgccggctag aatcggtctg ctcgcccaag 2340

.......... ...... ORF 1 ORF 1 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg 2400

2341 ccgggtaagc ctggcgttcc ttagccagtt atgtgatgta ccgcactaaa gtatacgcgc 2400

.......... ...... ORF 1 ORF 1 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc 2460

2401 taacgactag gggtacacat agtgaccgtt tgacactacc tgctgtggca gtcacgcagg 2460

.......... ...... ORF 1 ORF 1 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac 2520

2461 cagcgcgtcc gagagctact cgactacgaa acccggctcc tgacggggct tcaggccgtg 2520

.......... ...... ORF 1 ORF 1 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg 2580

2521 gagcacgtgc gcctaaagcc gaggttgtta caggactgcc tgttaccggc gtattgtcgc 2580

.......... ...... ORF 1 ORF 1 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc 2640

2581 cagtaactga cctcgctccg ctacaagccc ctaagggtta tgctccagcg gttgtagaag 2640

.......... ...... ORF 1 ORF 1 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat 2700

2641 aagacctccg gcaccaaccg aacatacctc gtcgtctgcg cgatgaagct cgcctccgta 2700

.......... ...... ORF 1 ORF 1 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa 2760

2701 ggcctcgaac gtcctagcgg cgccgaggcc cgcatatacg aggcgtaacc agaactggtt 2760

.......... ......... ORF 1 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc 2820

2761 gagatagtct cgaaccaact gccgttaaag ctactacgtc gaacccgcgt cccagctacg 2820

.......... ......... ORF 1 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc 2880

2821 ctgcgttagc aggctaggcc tcggccctga cagcccgcat gtgtttagcg ggcgtcttcg 2880

.......... ......... ORF 1 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc 2940

2881 cgccggcaga cctggctacc gacacatctt catgagcggc tatcaccttt ggctgcgggg 2940

.......... ......... ORF 1 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 agcactcgtc cgagggcaaa ggaatagagt agatgccgac cgaacaagag ctgatttcga 3000

2941 tcgtgagcag gctcccgttt ccttatctca tctacggctg gcttgttctc gactaaagct 3000

......... ORF 1 hygroB ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gaacgcctca gccagcaact cgcgcgagcc tagcaaggca aatgcgagag aacggcctta 3060

3001 cttgcggagt cggtcgttga gcgcgctcgg atcgttccgt ttacgctctc ttgccggaat 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgcttggtgg cacagttctc gtccacagtt cgctaagctc gctcggctgg gtcgcgggag 3120

3061 gcgaaccacc gtgtcaagag caggtgtcaa gcgattcgag cgagccgacc cagcgccctc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggccggtcgc agtgattcag gcccttctgg attgtgttgg tccccagggc acgattgtca 3180

3121 ccggccagcg tcactaagtc cgggaagacc taacacaacc aggggtcccg tgctaacagt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgcccacgca ctcgggtgat ctgactgatc ccgcagattg gagatcgccg cccgtgcctg 3240

3181 acgggtgcgt gagcccacta gactgactag ggcgtctaac ctctagcggc gggcacggac 3240

.......... .......... .......... .......... .......... ..........

.......... XbaI(3256) BclI(3266) .......... .......... ..........

3241 ccgattgggt gcagatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt 3300

3241 ggctaaccca cgtctagatc tattgactag tattagtcgg tatggtgtaa acatctccaa 3300

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

3301 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca 3360

3301 aatgaacgaa attttttgga gggtgtggag ggggacttgg actttgtatt ttacttacgt 3360

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 3420

3361 taacaacaac aattgaacaa ataacgtcga atattaccaa tgtttatttc gttatcgtag 3420

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 3480

3421 tgtttaaagt gtttatttcg taaaaaaagt gacgtaagat caacaccaaa caggtttgag 3480

.......... ......... SV40_PA_term ......... ......... EBV_rev_pri

.......... XhoI(3498) .......... .......... .......... ..........

3481 atcaatgtat cttaacgctc gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt 3540

3481 tagttacata gaattgcgag ctcacgtaag atcaacacca aacaggtttg agtagttaca 3540

SV40_PA_term ........ .......... ......... EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

3541 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 3600

3541 tagaatagta cagacatatg gcagctggag atcgatctcg aaccgcatta gtaccagtat 3600

.......... .......... .......... .......... ....... M13_reverse_pri

.......... .......... .......... .......... .......... ..........

3601 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 3660

3601 cgacaaagga cacactttaa caataggcga gtgttaaggt gtgttgtatg ctcggccttc 3660

M13_reverse_pri M13_pUC_rev_pri ........ .......... lac_pro

.......... .......... .......... .......... .......... ..........

3661 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 3720

3661 gtatttcaca tttcggaccc cacggattac tcactcgatt gagtgtaatt aacgcaacgc 3720

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 3780

3721 gagtgacggg cgaaaggtca gccctttgga cagcacggtc gacgtaatta cttagccggt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 3840

3781 tgcgcgcccc tctccgccaa acgcataacc cgcgagaagg cgaaggagcg agtgactgag 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 3900

3841 cgacgcgagc cagcaagccg acgccgctcg ccatagtcga gtgagtttcc gccattatgc 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 3960

3901 caataggtgt cttagtcccc tattgcgtcc tttcttgtac actcgttttc cggtcgtttt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 4020

3961 ccggtccttg gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg cggggggact 4020

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

4021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 4080

4021 gctcgtagtg tttttagctg cgagttcagt ctccaccgct ttgggctgtc ctgatatttc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 4140

4081 tatggtccgc aaagggggac cttcgaggga gcacgcgaga ggacaaggct gggacggcga 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg 4200

4141 atggcctatg gacaggcgga aagagggaag cccttcgcac cgcgaaagag ttacgagtgc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 4260

4201 gacatccata gagtcaagcc acatccagca agcgaggttc gacccgacac acgtgcttgg 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 4320

4261 ggggcaagtc gggctggcga cgcggaatag gccattgata gcagaactca ggttgggcca 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 4380

4321 ttctgtgctg aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 4440

4381 acatccgcca cgatgtctca agaacttcac caccggattg atgccgatgt gatcttcctg 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 4500

4441 tcataaacca tagacgcgag acgacttcgg tcaatggaag cctttttctc aaccatcgag 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 4560

4501 aactaggccg tttgtttggt ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 4620

4561 atgcgcgtct ttttttccta gagttcttct aggaaactag aaaagatgcc ccagactgcg 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 4680

4621 agtcaccttg cttttgagtg caattcccta aaaccagtac tctaatagtt tttcctagaa 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 4740

4681 gtggatctag gaaaatttaa tttttacttc aaaatttagt tagatttcat atatactcat 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 4800

4741 ttgaaccaga ctgtcaatgg ttacgaatta gtcactccgt ggatagagtc gctagacaga 4800

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4801 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 4860

4801 taaagcaagt aggtatcaac ggactgaggg gcagcacatc tattgatgct atgccctccc 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 4920

4861 gaatggtaga ccggggtcac gacgttacta tggcgctctg ggtgcgagtg gccgaggtct 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 4980

4921 aaatagtcgt tatttggtcg gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 5040

4981 taggcggagg taggtcagat aattaacaac ggcccttcga tctcattcat caagcggtca 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... FspI(5051) .......... .......... .......... ..........

5041 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 5100

5041 attatcaaac gcgttgcaac aacggtaacg atgtccgtag caccacagtg cgagcagcaa 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 5160

5101 accataccga agtaagtcga ggccaagggt tgctagttcc gctcaatgta ctagggggta 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 5220

5161 caacacgttt tttcgccaat cgaggaagcc aggaggctag caacagtctt cattcaaccg 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 5280

5221 gcgtcacaat agtgagtacc aataccgtcg tgacgtatta agagaatgac agtacggtag 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 5340

5281 gcattctacg aaaagacact gaccactcat gagttggttc agtaagactc ttatcacata 5340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 5400

5341 cgccgctggc tcaacgagaa cgggccgcag ttatgcccta ttatggcgcg gtgtatcgtc 5400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 5460

5401 ttgaaatttt cacgagtagt aaccttttgc aagaagcccc gcttttgaga gttcctagaa 5460

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 5520

5461 tggcgacaac tctaggtcaa gctacattgg gtgagcacgt gggttgacta gaagtcgtag 5520

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 5580

5521 aaaatgaaag tggtcgcaaa gacccactcg tttttgtcct tccgttttac ggcgtttttt 5580

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 5640

5581 cccttattcc cgctgtgcct ttacaactta tgagtatgag aaggaaaaag ttataataac 5640

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 5700

5641 ttcgtaaata gtcccaataa cagagtactc gcctatgtat aaacttacat aaatcttttt 5700

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5701 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc........ 5752

5701 atttgtttat ccccaaggcg cgtgtaaagg ggcttttcac ggtggactgc ag........ 5752

.......... .......... .......... .......... .......... ..........