Sequence of pIRESpuro3

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Sequence of pIRESpuro3

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Sequence of pIRESpuro3 : 5157 BP; 1206 A; 1417 C; 1305 G; 1229 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaaccatgg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

..... ClaI(910),EcoRV(914) NheI(927) AflII(934) ..... ..........

901 gagctcggat cgatatctgc ggcctagcta gcgcttaagg cctgttaacc ggtcgtacgt 960

901 ctcgagccta gctatagacg ccggatcgat cgcgaattcc ggacaattgg ccagcatgca 960

.......... .......... .......... .......... .......... ..........

BstBI(969),EcoRI(971) BamHI(977) NotI(984) ........ ..........

961 ctccggattc gaattcggat ccgcggccgc atagataact gatccagtgt gctggaatta 1020

961 gaggcctaag cttaagccta ggcgccggcg tatctattga ctaggtcaca cgaccttaat 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 attcgctgtc tgcgagggcc agctgttggg gtgagtactc cctctcaaaa gcgggcatga 1080

1021 taagcgacag acgctcccgg tcgacaaccc cactcatgag ggagagtttt cgcccgtact 1080

.......... .......... synth_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg 1140

1081 gaagacgcga ttctaacagt caaaggtttt tgctcctcct aaactataag tggaccgggc 1140

.......... .......... synth_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga aaagacaatc tttttgttgt 1200

1141 gccactacgg aaactcccac cggcgcaggt agaccagtct tttctgttag aaaaacaaca 1200

.......... .......... synth_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 caagcttgag gtgtggcagg cttgagatct ggccatacac ttgagtgaca atgacatcca 1260

1201 gttcgaactc cacaccgtcc gaactctaga ccggtatgtg aactcactgt tactgtaggt 1260

.......... .......... synth_int .......... .......... ..........

.......... .......... .......... .......... PstI(1305) ..........

1261 ctttgccttt ctctccacag gtgtccactc ccaggtccaa ctgcaggtcg agcatgcatc 1320

1261 gaaacggaaa gagaggtgtc cacaggtgag ggtccaggtt gacgtccagc tcgtacgtag 1320

.......... .......... synth_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tagggcggcc aattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc 1380

1321 atcccgccgg ttaaggcggg gagagggagg ggggggggat tgcaatgacc ggcttcggcg 1380

.......... .......... .......... IRES .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ttggaataag gccggtgtgc gtttgtctat atgtgatttt ccaccatatt gccgtctttt 1440

1381 aaccttattc cggccacacg caaacagata tacactaaaa ggtggtataa cggcagaaaa 1440

.......... .......... IRES .......... .......... ..........

.......... ApaI(1455) .......... .......... .......... ..........

1441 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt 1500

1441 ccgttacact cccgggcctt tggaccggga cagaagaact gctcgtaagg atccccagaa 1500

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg 1560

1501 aggggagagc ggtttcctta cgttccagac aacttacagc acttccttcg tcaaggagac 1560

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca 1620

1561 cttcgaagaa cttctgtttg ttgcagacat cgctgggaaa cgtccgtcgc cttggggggt 1620

.......... .......... IRES .......... .......... ..........

.......... .......... .......... PmlI(1654) .......... ..........

1621 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg 1680

1621 ggaccgctgt ccacggagac gccggttttc ggtgcacata ttctatgtgg acgtttccgc 1680

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc 1740

1681 cgtgttgggg tcacggtgca acactcaacc tatcaacacc tttctcagtt taccgagagg 1740

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct 1800

1741 agttcgcata agttgttccc cgacttccta cgggtcttcc atggggtaac ataccctaga 1800

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta 1860

1801 ctagaccccg gagccacgtg tacgaaatgt acacaaatca gctccaattt ttttgcagat 1860

.......... .......... IRES ORF 3 ORF 3 ..........

.......... .......... .......... .......... .......... ..........

1861 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac 1920

1861 ccggggggct tggtgcccct gcaccaaaag gaaacttttt gtgctactat tcgaacggtg 1920

......... IRES ORF 3 ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aacccacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc 1980

1921 ttgggtgttc ctctgctgga aggtactggc tcatgttcgg gtgccacgcg gagcggtggg 1980

.......... ......... ORF 3 ORF 3 ........ puro(0-600) .........

.......... .......... .......... .......... .......... ..........

1981 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca 2040

1981 cgctgctgca gggggcccgg catgcgtggg agcggcggcg caagcggctg atggggcggt 2040

.......... ... ORF 3 ORF 3 puro(0-600) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct 2100

2041 gcgcggtgtg gcagctgggc ctggcggtgt agctcgccca gtggctcgac gttcttgaga 2100

.......... ... ORF 3 ORF 3 puro(0-600) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg 2160

2101 aggagtgcgc gcagcccgag ctgtagccgt tccacaccca gcgcctgctg ccgcggcgcc 2160

.......... ... ORF 3 ORF 3 puro(0-600) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc 2220

2161 accgccagac ctggtgcggc ctctcgcagc ttcgcccccg ccacaagcgg ctctagccgg 2220

.......... ... ORF 3 ORF 3 puro(0-600) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc 2280

2221 gcgcgtaccg gctcaactcg ccaagggccg accggcgcgt cgttgtctac cttccggagg 2280

.......... ... ORF 3 ORF 3 puro(0-600) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg 2340

2281 accgcggcgt ggccgggttc ctcgggcgca ccaaggaccg gtggcagccg cagagcgggc 2340

.......... ... ORF 3 ORF 3 puro(0-600) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc 2400

2341 tggtggtccc gttcccagac ccgtcgcggc agcacgaggg gcctcacctc cgccggctcg 2400

.......... ... ORF 3 ORF 3 puro(0-600) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc 2460

2401 cgcggcccca cgggcggaag gacctctgga ggcgcggggc gttggagggg aagatgctcg 2460

.......... ... ORF 3 ORF 3 puro(0-600) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggctcggctt caccgtcacc gccgacgtcg agtgcccgaa ggaccgcgcg acctggtgca 2520

2461 ccgagccgaa gtggcagtgg cggctgcagc tcacgggctt cctggcgcgc tggaccacgt 2520

.......... ... ORF 3 ORF 3 puro(0-600) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga 2580

2521 actgggcgtt cgggccacgg actgcgggcg gggtgctggg cgtcgcgggc tggctttcct 2580

...... ORF 3 puro(0-600) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcgcacgacc ccatggctcc gaccgaagcc gacccgggcg gccccgccga ccccgcaccc 2640

2581 cgcgtgctgg ggtaccgagg ctggcttcgg ctgggcccgc cggggcggct ggggcgtggg 2640

ORF 3 .......... .......... .......... .......... ..........

.......... XbaI(2660) BclI(2670) .......... .......... ..........

2641 gcccccgagg cccaccgact ctagataact gatcataatc agccatacca catttgtaga 2700

2641 cgggggctcc gggtggctga gatctattga ctagtattag tcggtatggt gtaaacatct 2700

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

2701 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 2760

2701 ccaaaatgaa cgaaattttt tggagggtgt ggagggggac ttggactttg tattttactt 2760

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 2820

2761 acgttaacaa caacaattga acaaataacg tcgaatatta ccaatgttta tttcgttatc 2820

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 2880

2821 gtagtgttta aagtgtttat ttcgtaaaaa aagtgacgta agatcaacac caaacaggtt 2880

.......... ......... SV40_PA_term ......... ......... EBV_rev_pri

.......... .......... .......... .......... .......... ..........

2881 actcatcaat gtatcttaac gcgtcgagtg cattctagtt gtggtttgtc caaactcatc 2940

2881 tgagtagtta catagaattg cgcagctcac gtaagatcaa caccaaacag gtttgagtag 2940

SV40_PA_term ........ .......... ......... EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

2941 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 3000

2941 ttacatagaa tagtacagac atatggcagc tggagatcga tctcgaaccg cattagtacc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 3060

3001 agtatcgaca aaggacacac tttaacaata ggcgagtgtt aaggtgtgtt gtatgctcgg 3060

M13_reverse_pri . M13_pUC_rev_pri ....... .......... lac_pro

.......... .......... .......... .......... .......... ..........

3061 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 3120

3061 ccttcgtatt tcacatttcg gaccccacgg attactcact cgattgagtg taattaacgc 3120

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 3180

3121 aacgcgagtg acgggcgaaa ggtcagccct ttggacagca cggtcgacgt aattacttag 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 3240

3181 ccggttgcgc gcccctctcc gccaaacgca taacccgcga gaaggcgaag gagcgagtga 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 3300

3241 ctgagcgacg cgagccagca agccgacgcc gctcgccata gtcgagtgag tttccgccat 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 3360

3301 tatgccaata ggtgtcttag tcccctattg cgtcctttct tgtacactcg ttttccggtc 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 3420

3361 gttttccggt ccttggcatt tttccggcgc aacgaccgca aaaaggtatc cgaggcgggg 3420

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 3480

3421 ggactgctcg tagtgttttt agctgcgagt tcagtctcca ccgctttggg ctgtcctgat 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 3540

3481 atttctatgg tccgcaaagg gggaccttcg agggagcacg cgagaggaca aggctgggac 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 3600

3541 ggcgaatggc ctatggacag gcggaaagag ggaagccctt cgcaccgcga aagagttacg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 3660

3601 agtgcgacat ccatagagtc aagccacatc cagcaagcga ggttcgaccc gacacacgtg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 3720

3661 cttggggggc aagtcgggct ggcgacgcgg aataggccat tgatagcaga actcaggttg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 3780

3721 ggccattctg tgctgaatag cggtgaccgt cgtcggtgac cattgtccta atcgtctcgc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 3840

3781 tccatacatc cgccacgatg tctcaagaac ttcaccaccg gattgatgcc gatgtgatct 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 3900

3841 tcctgtcata aaccatagac gcgagacgac ttcggtcaat ggaagccttt ttctcaacca 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 3960

3901 tcgagaacta ggccgtttgt ttggtggcga ccatcgccac caaaaaaaca aacgttcgtc 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 4020

3961 gtctaatgcg cgtctttttt tcctagagtt cttctaggaa actagaaaag atgccccaga 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 4080

4021 ctgcgagtca ccttgctttt gagtgcaatt ccctaaaacc agtactctaa tagtttttcc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 4140

4081 tagaagtgga tctaggaaaa tttaattttt acttcaaaat ttagttagat ttcatatata 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 4200

4141 ctcatttgaa ccagactgtc aatggttacg aattagtcac tccgtggata gagtcgctag 4200

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 4260

4201 acagataaag caagtaggta tcaacggact gaggggcagc acatctattg atgctatgcc 4260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 4320

4261 ctcccgaatg gtagaccggg gtcacgacgt tactatggcg ctctgggtgc gagtggccga 4320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 4380

4321 ggtctaaata gtcgttattt ggtcggtcgg ccttcccggc tcgcgtcttc accaggacgt 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 4440

4381 tgaaataggc ggaggtaggt cagataatta acaacggccc ttcgatctca ttcatcaagc 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... FspI(4456) .......... .......... .......... ..........

4441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 4500

4441 ggtcaattat caaacgcgtt gcaacaacgg taacgatgtc cgtagcacca cagtgcgagc 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 4560

4501 agcaaaccat accgaagtaa gtcgaggcca agggttgcta gttccgctca atgtactagg 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 4620

4561 gggtacaaca cgttttttcg ccaatcgagg aagccaggag gctagcaaca gtcttcattc 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 4680

4621 aaccggcgtc acaatagtga gtaccaatac cgtcgtgacg tattaagaga atgacagtac 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 4740

4681 ggtaggcatt ctacgaaaag acactgacca ctcatgagtt ggttcagtaa gactcttatc 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 4800

4741 acatacgccg ctggctcaac gagaacgggc cgcagttatg ccctattatg gcgcggtgta 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 4860

4801 tcgtcttgaa attttcacga gtagtaacct tttgcaagaa gccccgcttt tgagagttcc 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 4920

4861 tagaatggcg acaactctag gtcaagctac attgggtgag cacgtgggtt gactagaagt 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 4980

4921 cgtagaaaat gaaagtggtc gcaaagaccc actcgttttt gtccttccgt tttacggcgt 4980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 5040

4981 tttttccctt attcccgctg tgcctttaca acttatgagt atgagaagga aaaagttata 5040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 5100

5041 ataacttcgt aaatagtccc aataacagag tactcgccta tgtataaact tacataaatc 5100

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc... 5157

5101 tttttatttg tttatcccca aggcgcgtgt aaaggggctt ttcacggtgg actgcag... 5157

.......... .......... .......... .......... .......... ..........