r\r\&LOCUS pIS0 5264 bp DNA SYN 28-Aug-2007 DEFINITION pIS0 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5264 /organism="pIS0" /mol_type="other DNA" rep_origin 63..140 /label="SV40_origin" promoter 16..218 /label="SV40_promoter" misc_feature 125..144 /label="SV40pro_F_primer" misc_feature complement(314..332) /label="LucNrev_primer" CDS 248..1900 /label="ORF frame 2" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*" misc_feature 248..1900 /label="firefly_Luciferase" misc_feature complement(1992..2011) /label="EBV_rev_primer" terminator complement(1978..2097) /label="SV40_PA_terminator" misc_feature 2217..2444 /label="SV40_enhancer" misc_feature 2424..2444 /label="pBABE_3_primer" rep_origin complement(2752..3371) /label="pBR322_origin" CDS complement(3526..4386) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(3526..4386) /label="Ampicillin" /gene="Ampicillin" promoter complement(4428..4456) /label="AmpR_promoter" rep_origin 4535..4841 /label="f1_origin" terminator 5104..5152 /label="synth_PA_terminator" misc_feature 5104..5257 /label="TB" terminator 5104..5257 /label="up_PA_terminator" misc_feature 5206..5225 /label="RVprimer3_primer" ORIGIN 1 ggtagatctg cgatctgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 61 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatcgc tgactaattt 121 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 181 gaggcttttt tggaggccta ggcttttgca aaaagcttgg cattccggta ctgttggtaa 241 agccaccatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga 301 agatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg 361 aacaattgct tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga 421 aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat 481 cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat 541 cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat 601 gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa 661 cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta 721 ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga 781 atacgatttt gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc 841 ctctggatct actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag 901 attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt 961 aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat atttgatatg 1021 tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca 1081 ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag 1141 cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc 1201 cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca 1261 aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa 1321 accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac 1381 cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat 1441 gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct 1501 acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct 1561 gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt 1621 gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg 1681 tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat 1741 cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt 1801 tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat 1861 cctcataaag gccaagaagg gcggaaagat cgccgtgtaa ttctaggagc tctatacgcg 1921 tctcaagctt actgctagcg ttctagagtc ggggcggccg gccgcttcga gcagacatga 1981 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta 2041 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 2101 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt 2161 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctg aacgatggag 2221 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac 2281 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg 2341 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc 2401 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagcg gatccgtcga 2461 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact 2521 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca 2581 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 2641 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 2701 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 2761 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 2821 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 2881 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 2941 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 3001 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 3061 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 3121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 3181 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 3241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 3301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 3361 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 3421 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 3481 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 3541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 3601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 3661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 3721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 3781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 3841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 3901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 3961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 4021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 4081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 4141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 4201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 4261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 4321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 4381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 4441 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 4501 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 4561 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 4621 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 4681 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 4741 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 4801 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 4861 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 4921 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttgccattc 4981 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 5041 ccagcccaag ctaccatgat aagtaagtaa tattaaggta cgggaggtac ttggagcggc 5101 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata 5161 gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct 5221 gtccccagtg caagtgcagg tgccagaaca tttctctatc gata //pIS0 "Times New Romanr\C? SV40_origin"Times New Romanr\CSV40_pro"Times New Roman<r\CɆ} SV40pro_F_pri"Times New Romanr\ClORF2"Times New Romanr\Clfirefly_Luciferase"Times New Roman<r\:L LucNrev_pri"Times New Romanr\1 SV40_PA_term"Times New Roman<r\CɆ EBV_rev_pri"Times New Romanr\C SV40_enh"Times New Romanr\Cx  pBABE_3_pri"Times New Romanr\CɆ +  pBR322_origin"Times New Romanr\ "ORF1"Times New Roman<r\C " Ampicillin"Times New Romanr\CLhAmpR_pro"Times New Romanr\CɆ f1_origin"Times New Romanr\  synth_PA_term"Times New Romanr\CTB"Times New Romanr\C up_PA_term"Times New Roman<r\CɆVi RVprimer3_pri"Times New Roman r\)BglII(4), StuI(196), NcoI(246), NarI(281)"Times New Roman1H r\BstBI"Times New Roman $- r\BclI"Times New Roman< K[kk r\"SacI(1911), NheI(1934), XbaI(1942)"Times New Roman+8G]!] 7 r\HpaI"Times New Roman6#.%>1M1Mq\ r\BamHI(2450), SalI(2456)"Times New Roman Uhwqw r\AseI"Times New Roman W r\NotI"Times New Roman*gQBB r\test"Times New Romanzz r\test"Times New Roman?Off