Sequence of pIS0

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Sequence of pIS0

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Sequence of pIS0 : 5264 BP; 1341 A; 1267 C; 1280 G; 1376 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ggtagatctg cgatctgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 60

1 ccatctagac gctagacgta gagttaatca gtcgttggta tcagggcggg gattgaggcg 60

.......... .......... .......... SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatcgc tgactaattt 120

61 ggtagggcgg ggattgaggc gggtcaaggc gggtaagagg cggggtagcg actgattaaa 120

.......... ......... SV40_pro SV40_origin ......... ..........

.......... .......... .......... .......... .......... ..........

121 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 180

121 aaaaataaat acgtctccgg ctccggcgga gccggagact cgataaggtc ttcatcactc 180

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaggcttttt tggaggccta ggcttttgca aaaagcttgg cattccggta ctgttggtaa 240

181 ctccgaaaaa acctccggat ccgaaaacgt ttttcgaacc gtaaggccat gacaaccatt 240

.......... SV40_pro .......... .......... .......... ..........

NcoI(246) .......... .......... .......... NarI(281) ..........

241 agccaccatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga 300

241 tcggtggtac cttctgcggt ttttgtattt ctttccgggc cgcggtaaga taggcgacct 300

.......... .......... ... ORF2 firefly_Luciferase ... ..........

.......... .......... .......... .......... .......... ..........

301 agatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg 360

301 tctaccttgg cgacctctcg ttgacgtatt ccgatacttc tctatgcggg accaaggacc 360

....... ORF2 firefly_Luciferase LucNrev_pri ....... ..........

.......... .......... .......... .......... .......... BstBI(417)

361 aacaattgct tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga 420

361 ttgttaacga aaatgtctac gtgtatagct ccacctgtag tgaatgcgac tcatgaagct 420

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat 480

421 ttacaggcaa gccaaccgtc ttcgatactt tgctataccc gacttatgtt tagtgtctta 480

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat 540

481 gcagcatacg tcacttttga gagaagttaa gaaatacggc cacaacccgc gcaataaata 540

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat 600

541 gcctcaacgt caacgcgggc gcttgctgta aatattactt gcacttaacg agttgtcata 600

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa 660

601 cccgtaaagc gtcggatggc accacaagca aaggtttttc cccaacgttt tttaaaactt 660

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta 720

661 gcacgttttt ttcgagggtt agtaggtttt ttaataatag tacctaagat tttgcctaat 720

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga 780

721 ggtccctaaa gtcagctaca tgtgcaagca gtgtagagta gatggagggc caaaattact 780

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... BclI(828) ..........

781 atacgatttt gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc 840

781 tatgctaaaa cacggtctca ggaagctatc cctgttctgt taacgtgact agtacttgag 840

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctctggatct actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag 900

841 gagacctaga tgaccagacg gatttccaca gcgagacgga gtatcttgac ggacgcactc 900

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

901 attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt 960

901 taagagcgta cggtctctag gataaaaacc gttagtttag taaggcctat gacgctaaaa 960

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat atttgatatg 1020

961 ttcacaacaa ggtaaggtag tgccaaaacc ttacaaatga tgtgagccta taaactatac 1020

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca 1080

1021 acctaaagct cagcagaatt acatatctaa acttcttctc gacaaagact cctcggaagt 1080

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag 1140

1081 cctaatgttc taagtttcac gcgacgacca cggttgggat aagaggaaga agcggttttc 1140

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc 1200

1141 gtgagactaa ctgtttatgc taaatagatt aaatgtgctt taacgaagac caccgcgagg 1200

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca 1260

1201 ggagagattc cttcagcccc ttcgccaacg gttctccaag gtagacggtc catagtccgt 1260

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa 1320

1261 tcctataccc gagtgactct gatgtagtcg ataagactaa tgtgggctcc ccctactatt 1320

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac 1380

1321 tggcccgcgc cagccatttc aacaaggtaa aaaacttcgc ttccaacacc tagacctatg 1380

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat 1440

1381 gcccttttgc gacccgcaat tagtttctcc gcttgacaca cactctccag gatactaata 1440

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct 1500

1441 caggccaata catttgttag gccttcgctg gttgcggaac taactgttcc tacctaccga 1500

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct 1560

1501 tgtaagacct ctgtatcgaa tgaccctgct tctgcttgtg aagaagtagc aactggcgga 1560

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt 1620

1561 cttcagagac taattcatgt ttccgatagt ccaccgaggg cgacttaacc ttaggtagaa 1620

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg 1680

1621 cgaggttgtg gggttgtaga agctgcgtcc acagcgtcca gaagggctgc tactgcggcc 1680

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat 1740

1681 acttgaaggg cggcggcaac aacaaaacct cgtgcctttc tgctactgcc tttttctcta 1740

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt 1800

1741 gcacctaatg cagcggtcag ttcattgttg gcgctttttc aacgcgcctc ctcaacacaa 1800

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat 1860

1801 acacctgctt catggctttc cagaatggcc ttttgagctg cgttcttttt agtctctcta 1860

.......... ... ORF2 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cctcataaag gccaagaagg gcggaaagat cgccgtgtaa ttctaggagc tctatacgcg 1920

1861 ggagtatttc cggttcttcc cgcctttcta gcggcacatt aagatcctcg agatatgcgc 1920

... ORF2 firefly_Luciferase ... .......... .......... ..........

.......... NheI(1934) XbaI(1942) .......... .......... ..........

1921 tctcaagctt actgctagcg ttctagagtc ggggcggccg gccgcttcga gcagacatga 1980

1921 agagttcgaa tgacgatcgc aagatctcag ccccgccggc cggcgaagct cgtctgtact 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta 2040

1981 attctatgta actactcaaa cctgtttggt gttgatctta cgtcactttt tttacgaaat 2040

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 2100

2041 aaacacttta aacactacga taacgaaata aacattggta atattcgacg ttatttgttc 2100

.......... ......... SV40_PA_term ......... .......... ..........

HpaI(2102) .......... .......... .......... .......... ..........

2101 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt 2160

2101 aattgttgtt gttaacgtaa gtaaaataca aagtccaagt ccccctccac accctccaaa 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctg aacgatggag 2220

2161 aaatttcgtt cattttggag atgtttacac cattttagct attcctagac ttgctacctc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac 2280

2221 gcctcttacc cgccttgacc cgcctcaatc cccgccctac ccgcctcaat ccccgccctg 2280

.......... .......... SV40_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg 2340

2281 ataccaacga ctgattaact ctacgtacga aacgtatgaa gacggacgac ccctcggacc 2340

.......... .......... SV40_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc 2400

2341 cctgaaaggt gtggaccaac gactgattaa ctctacgtac gaaacgtatg aagacggacg 2400

.......... .......... SV40_enh .......... .......... ..........

.......... .......... .......... ......... BamHI(2450) SalI(2456)

2401 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagcg gatccgtcga 2460

2401 acccctcgga cccctgaaag gtgtgggatt gactgtgtgt aaggtgtcgc ctaggcagct 2460

.......... ......... SV40_enh pBABE_3_pri ......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact 2520

2461 ggctacggga actctcggaa gttgggtcag tcgaggaagg ccacccgcgc cccgtactga 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca 2580

2521 tagcagcggc gtgaatactg acagaagaaa tagtacgttg agcatcctgt ccacggccgt 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 2640

2581 cgcgagaagg cgaaggagcg agtgactgag cgacgcgagc cagcaagccg acgccgctcg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 2700

2641 ccatagtcga gtgagtttcc gccattatgc caataggtgt cttagtcccc tattgcgtcc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 2760

2701 tttcttgtac actcgttttc cggtcgtttt ccggtccttg gcatttttcc ggcgcaacga 2760

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

2761 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 2820

2761 ccgcaaaaag gtatccgagg cggggggact gctcgtagtg tttttagctg cgagttcagt 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 2880

2821 ctccaccgct ttgggctgtc ctgatatttc tatggtccgc aaagggggac cttcgaggga 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 2940

2881 gcacgcgaga ggacaaggct gggacggcga atggcctatg gacaggcgga aagagggaag 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 3000

2941 cccttcgcac cgcgaaagag tatcgagtgc gacatccata gagtcaagcc acatccagca 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 3060

3001 agcgaggttc gacccgacac acgtgcttgg ggggcaagtc gggctggcga cgcggaatag 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 3120

3061 gccattgata gcagaactca ggttgggcca ttctgtgctg aatagcggtg accgtcgtcg 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 3180

3121 gtgaccattg tcctaatcgt ctcgctccat acatccgcca cgatgtctca agaacttcac 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 3240

3181 caccggattg atgccgatgt gatcttcttg tcataaacca tagacgcgag acgacttcgg 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 3300

3241 tcaatggaag cctttttctc aaccatcgag aactaggccg tttgtttggt ggcgaccatc 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 3360

3301 gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct ttttttccta gagttcttct 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 3420

3361 aggaaactag aaaagatgcc ccagactgcg agtcaccttg cttttgagtg caattcccta 3420

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 3480

3421 aaaccagtac tctaatagtt tttcctagaa gtggatctag gaaaatttaa tttttacttc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 3540

3481 aaaatttagt tagatttcat atatactcat ttgaaccaga ctgtcaatgg ttacgaatta 3540

.......... .......... .......... .......... ....... ORF1 Ampicillin

.......... .......... .......... .......... .......... ..........

3541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 3600

3541 gtcactccgt ggatagagtc gctagacaga taaagcaagt aggtatcaac ggactgaggg 3600

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 3660

3601 gcagcacatc tattgatgct atgccctccc gaatggtaga ccggggtcac gacgttacta 3660

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 3720

3661 tggcgctctg ggtgcgagtg gccgaggtct aaatagtcgt tatttggtcg gtcggccttc 3720

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... AseI(3771)

3721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 3780

3721 ccggctcgcg tcttcaccag gacgttgaaa taggcggagg taggtcagat aattaacaac 3780

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 3840

3781 ggcccttcga tctcattcat caagcggtca attatcaaac gcgttgcaac aacggtaacg 3840

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 3900

3841 atgtccgtag caccacagtg cgagcagcaa accataccga agtaagtcga ggccaagggt 3900

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 3960

3901 tgctagttcc gctcaatgta ctagggggta caacacgttt tttcgccaat cgaggaagcc 3960

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 4020

3961 aggaggctag caacagtctt cattcaaccg gcgtcacaat agtgagtacc aataccgtcg 4020

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 4080

4021 tgacgtatta agagaatgac agtacggtag gcattctacg aaaagacact gaccactcat 4080

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 4140

4081 gagttggttc agtaagactc ttatcacata cgccgctggc tcaacgagaa cgggccgcag 4140

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 4200

4141 ttatgcccta ttatggcgcg gtgtatcgtc ttgaaatttt cacgagtagt aaccttttgc 4200

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 4260

4201 aagaagcccc gcttttgaga gttcctagaa tggcgacaac tctaggtcaa gctacattgg 4260

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 4320

4261 gtgagcacgt gggttgacta gaagtcgtag aaaatgaaag tggtcgcaaa gacccactcg 4320

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 4380

4321 tttttgtcct tccgttttac ggcgtttttt cccttattcc cgctgtgcct ttacaactta 4380

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 4440

4381 tgagtatgag aaggaaaaag ttataataac ttcgtaaata gtcccaataa cagagtactc 4440

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

4441 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 4500

4441 gcctatgtat aaacttacat aaatcttttt atttgtttat ccccaaggcg cgtgtaaagg 4500

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 4560

4501 ggcttttcac ggtggactgc gcgggacatc gccgcgtaat tcgcgccgcc cacaccacca 4560

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

4561 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 4620

4561 atgcgcgtcg cactggcgat gtgaacggtc gcgggatcgc gggcgaggaa agcgaaagaa 4620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 4680

4621 gggaaggaaa gagcggtgca agcggccgaa aggggcagtt cgagatttag cccccgaggg 4680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 4740

4681 aaatcccaag gctaaatcac gaaatgccgt ggagctgggg ttttttgaac taatcccact 4740

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 4800

4741 accaagtgca tcacccggta gcgggactat ctgccaaaaa gcgggaaact gcaacctcag 4800

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 4860

4801 gtgcaagaaa ttatcacctg agaacaaggt ttgaccttgt tgtgagttgg gatagagcca 4860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 4920

4861 gataagaaaa ctaaatattc cctaaaacgg ctaaagccgg ataaccaatt ttttactcga 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttgccattc 4980

4921 ctaaattgtt tttaaattgc gcttaaaatt gttttataat tgcgaatgtt aaacggtaag 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 5040

4981 cggtaagtcc gacgcgttga caacccttcc cgctagccac gcccggagaa gcgataatgc 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NotI(5097)

5041 ccagcccaag ctaccatgat aagtaagtaa tattaaggta cgggaggtac ttggagcggc 5100

5041 ggtcgggttc gatggtacta ttcattcatt ataattccat gccctccatg aacctcgccg 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata 5160

5101 gcgttatttt atagaaataa aagtaatgta gacacacaac caaaaaacac acttagctat 5160

.......... ...... synth_PA_term TB up_PA_term ...... ..........

.......... .......... .......... .......... .......... ..........

5161 gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct 5220

5161 catgattgta tgcgagaggt agttttgttt tgctttgttt tgtttgatcg ttttatccga 5220

.......... ........ TB up_PA_term ........ ........ RVprimer3_pri

.......... .......... .......... .......... .......... ..........

5221 gtccccagtg caagtgcagg tgccagaaca tttctctatc gata...... .......... 5264

5221 caggggtcac gttcacgtcc acggtcttgt aaagagatag ctat...... .......... 5264

........ TB up_PA_term ........ .......... .......... ..........