Sequence of pIS1

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Sequence of pIS1

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Sequence of pIS1 : 4085 BP; 1072 A; 906 C; 1049 G; 1058 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta 60

1 attgaccagg cgtcaccacc cggtctacat ttgtttactt acaagaacta agtaaataat 60

.......... .......... .......... ......... Orf frame 3 .........

.......... .......... .......... .......... .......... ..........

61 attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg 120

61 taataatact aagtcttttt gtacgtcttt tacgacaata aaaaaatgta ccattgcgcc 120

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cctcttctta tttatggcga catgttgtgc cacatattga gccagtagcg cggtgtatta 180

121 ggagaagaat aaataccgct gtacaacacg gtgtataact cggtcatcgc gccacataat 180

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac 240

181 atggtctgga ataaccatac ccgtttagtc cgtttagacc attaccaaga atatccaatg 240

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ttgatcatta caaatatctt actgcatggt ttgaacttct taatttacca aagaagatca 300

241 aactagtaat gtttatagaa tgacgtacca aacttgaaga attaaatggt ttcttctagt 300

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag 360

301 aaaaacagcc ggtactaacc ccacgaacaa accgtaaagt aatatcgata ctcgtagttc 360

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg 420

361 tattctagtt tcgttatcaa gtgcgacttt cacatcatct acactaactt agtaccctac 420

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg 480

421 ttaccggact ataacttctt ctataacgca actagtttag acttcttcct cttttttacc 480

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag 540

481 aaaacctctt attgaagaag cacctttggt acaacggtag tttttagtac tctttcaatc 540

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc 600

541 ttggtcttct taaacgtcgt atagaacttg gtaagtttct ctttccactt caagcagcag 600

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac 660

601 gttgtaatag taccggagca ctttagggca atcattttcc accatttgga ctgcaacatg 660

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta 720

661 tttaacaatc cttaatatta cgaatagatg cacgttcact actaaatggt ttttacaaat 720

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta 780

721 aacttagcct gggtcctaag aaaaggttac gataacaact tccacggttc ttcaaaggat 780

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa 840

781 tatgacttaa acagtttcat tttccagaag taaaaagcgt tcttctacgt ggactacttt 840

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taattctagg 900

841 acccttttat atagtttagc aagcaactcg ctcaagagtt tttacttgtt attaagatcc 900

.......... ......... Orf frame 3 ......... .......... ..........

SacI,AgeI EcoRV SpeI XbaI935) NotI(942),EagI(942)

901 agctctatac cggtctcgat atcactacta gtgttctaga gcggccgctt cgagcagaca 960

901 tcgagatatg gccagagcta tagtgatgat cacaagatct cgccggcgaa gctcgtctgt 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct 1020

961 actattctat gtaactactc aaacctgttt ggtgttgatc ttacgtcact ttttttacga 1020

........ SV40_PA_terminator EBV_rev_primer ........ ..........

.......... .......... .......... .......... .......... ..........

1021 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac 1080

1021 aataaacact ttaaacacta cgataacgaa ataaacattg gtaatattcg acgttatttg 1080

.......... ...... SV40_PA_terminator ...... .......... ..........

HpaI(1085) .......... .......... .......... .......... ..........

1081 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg 1140

1081 ttcaattgtt gttgttaacg taagtaaaat acaaagtcca agtccccctc cacaccctcc 1140

.......... .......... .......... .......... .......... ..........

.......... .......... ......... ClaI(1180) BamHI(1187) .........

1141 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccaggtggca 1200

1141 aaaaaatttc gttcattttg gagatgttta caccatttta gctattccta ggtccaccgt 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 1260

1201 gaaaagcccc tttacacgcg ccttggggat aaacaaataa aaagatttat gtaagtttat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 1320

1261 acataggcga gtactctgtt attgggacta tttacgaagt tattataact ttttccttct 1320

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 1380

1321 catactcata agttgtaaag gcacagcggg aataagggaa aaaacgccgt aaaacggaag 1380

.......... .......... .... Orf frame 3 Ampicillin .... ..........

.......... .......... .......... .......... .......... ..........

1381 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 1440

1381 gacaaaaacg agtgggtctt tgcgaccact ttcattttct acgacttcta gtcaacccac 1440

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 1500

1441 gtgctcaccc aatgtagctt gacctagagt tgtcgccatt ctaggaactc tcaaaagcgg 1500

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 1560

1501 ggcttcttgc aaaaggttac tactcgtgaa aatttcaaga cgatacaccg cgccataata 1560

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 1620

1561 gggcataact gcggcccgtt ctcgttgagc cagcggcgta tgtgataaga gtcttactga 1620

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 1680

1621 accaactcat gagtggtcag tgtcttttcg tagaatgcct accgtactgt cattctctta 1680

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 1740

1681 atacgtcacg acggtattgg tactcactat tgtgacgccg gttgaatgaa gactgttgct 1740

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 1800

1741 agcctcctgg cttcctcgat tggcgaaaaa acgtgttgta ccccctagta cattgagcgg 1800

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 1860

1801 aactagcaac ccttggcctc gacttacttc ggtatggttt gctgctcgca ctgtggtgct 1860

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... FspI(1887) .......... .......... ..........

1861 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 1920

1861 acggacatcg ttaccgttgt tgcaacgcgt ttgataattg accgcttgat gaatgagatc 1920

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 1980

1921 gaagggccgt tgttaattat ctgacctacc tccgcctatt tcaacgtcct ggtgaagacg 1980

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 2040

1981 cgagccggga aggccgaccg accaaataac gactatttag acctcggcca ctcgcaccca 2040

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 2100

2041 gagcgccata gtaacgtcgt gaccccggtc taccattcgg gagggcatag catcaataga 2100

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 2160

2101 tgtgctgccc ctcagtccgt tgatacctac ttgctttatc tgtctagcga ctctatccac 2160

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 2220

2161 ggagtgacta attcgtaacc attgacagtc tggttcaaat gagtatatat gaaatctaac 2220

.... Orf frame 3 Ampicillin .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 2280

2221 taaattttga agtaaaaatt aaattttcct agatccactt ctaggaaaaa ctattagagt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 2340

2281 actggtttta gggaattgca ctcaaaagca aggtgactcg cagtctgggg catcttttct 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 2400

2341 agtttcctag aagaactcta ggaaaaaaag acgcgcatta gacgacgaac gtttgttttt 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 2460

2401 ttggtggcga tggtcgccac caaacaaacg gcctagttct cgatggttga gaaaaaggct 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 2520

2461 tccattgacc gaagtcgtct cgcgtctatg gtttatgaca agaagatcac atcggcatca 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 2580

2521 atccggtggt gaagttcttg agacatcgtg gcggatgtat ggagcgagac gattaggaca 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 2640

2581 atggtcaccg acgacggtca ccgctattca gcacagaatg gcccaacctg agttctgcta 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 2700

2641 tcaatggcct attccgcgtc gccagcccga cttgcccccc aagcacgtgt gtcgggtcga 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 2760

2701 acctcgcttg ctggatgtgg cttgactcta tggatgtcgc actcgatact ctttcgcggt 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 2820

2761 gcgaagggct tccctctttc cgcctgtcca taggccattc gccgtcccag ccttgtcctc 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 2880

2821 tcgcgtgctc cctcgaaggt ccccctttgc ggaccataga aatatcagga cagcccaaag 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 2940

2881 cggtggagac tgaactcgca gctaaaaaca ctacgagcag tccccccgcc tcggatacct 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 3000

2941 ttttgcggtc gttgcgccgg aaaaatgcca aggaccggaa aacgaccgga aaacgagtgt 3000

pBR322_origin ...... .......... .......... .......... ..........

BglII(3010) ........ .......... .......... .......... ..........

3001 tggctcgaca gatctaaatg agtcttcgga cctcgcgggg gccgcttaag cggtggttag 3060

3001 accgagctgt ctagatttac tcagaagcct ggagcgcccc cggcgaattc gccaccaatc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggtttgtctg acgcgggggg agggggaagg aacgaaacac tctcattcgg aggcggctcg 3120

3061 ccaaacagac tgcgcccccc tcccccttcc ttgctttgtg agagtaagcc tccgccgagc 3120

.......... .......... .......... .......... .......... ..........

.......... MscI(3137) .......... .......... .......... ..........

3121 gggtttggtc ttggtggcca cgggcacgca gaagagcgcc gcgatcctct taagcacccc 3180

3121 cccaaaccag aaccaccggt gcccgtgcgt cttctcgcgg cgctaggaga attcgtgggg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cccgccctcc gtggaggcgg gggtttggtc ggcgggtggt aactggcggg ccgctgactc 3240

3181 gggcgggagg cacctccgcc cccaaaccag ccgcccacca ttgaccgccc ggcgactgag 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... SmaI(3293)

3241 gggcgggtcg cgcgccccag agtgtgacct tttcggtctg ctcgcagacc cccgggcggc 3300

3241 cccgcccagc gcgcggggtc tcacactgga aaagccagac gagcgtctgg gggcccgccg 3300

.......... .......... .......... .......... .......... ..........

SacII(3308) ........ .......... NcoI(3339) .......... ..........

3301 gccgccgcgg cggcgacggg ctcgctgggt cctaggctcc atggggaccg tatacgtgga 3360

3301 cggcggcgcc gccgctgccc gagcgaccca ggatccgagg tacccctggc atatgcacct 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 caggctctgg agcatccgca cgactgcggt gatattaccg gagaccttct gcgggacgag 3420

3361 gtccgagacc tcgtaggcgt gctgacgcca ctataatggc ctctggaaga cgccctgctc 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ccgggtcacg cggctgacgc ggagcgtccg ttgggcgaca aacaccagga cggggcacag 3480

3421 ggcccagtgc gccgactgcg cctcgcaggc aacccgctgt ttgtggtcct gccccgtgtc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... PvuII(3540)

3481 gtacactatc ttgtcacccg gaggcgcgag ggactgcagg agcttcaggg agtggcgcag 3540

3481 catgtgatag aacagtgggc ctccgcgctc cctgacgtcc tcgaagtccc tcaccgcgtc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata 3600

3541 gacgaagtag gggcaccggg caacgagcgc aaacgaccgc cacaggggcc ttctttatat 3600

.......... ....... TATA_promoter TK_promoter ....... ..........

.......... .......... .......... .......... ......... EcoRI(3658)

3601 tttgcatgtc tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa 3660

3601 aaacgtacag aaatcaagat actactgtgt ttggggcggg tcgcagaaca gtaaccgctt 3660

.......... .. TATA_promoter TK_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttcgaacacg cagatgcagt cggggcggcg cggtcccagg tccacttcgc atattaaggt 3720

3661 aagcttgtgc gtctacgtca gccccgccgc gccagggtcc aggtgaagcg tataattcca 3720

....... TATA_promoter TK_promoter ....... .......... ..........

.......... .......... .......... ........ HindIII(3769) ........

3721 gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaaa gcttgattct 3780

3721 ctgcgcacac cggagcttgt ggctcgctgg gacgtcgctg ggcgaatttt cgaactaaga 3780

.......... ......... TK_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tctgacacaa cagtctcgaa cttaagctgc agaagttggt cgtgaggcac tgggcaggta 3840

3781 agactgtgtt gtcagagctt gaattcgacg tcttcaacca gcactccgtg acccgtccat 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag 3900

3841 tcatagttcc aatgttctgt ccaaattcct ctggttatct ttgacccgaa cagctctgtc 3900

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt 3960

3901 tcttctgaga acgcaaagac tatccgtgga taaccagaat gactgtaggt gaaacggaaa 3960

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ctctccacag gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac 4020

3961 gagaggtgtc cacaggtgag ggtcaagtta atgtcgagaa ttccgatctc atgaattatg 4020

chim_int .......... .......... .......... .......... ..........

.......... NheI(4033) .......... .......... .......... ..........

4021 gactcactat aggctagcca ccatgacttc gaaagtttat gatccagaac aaaggaaacg 4080

4021 ctgagtgata tccgatcggt ggtactgaag ctttcaaata ctaggtcttg tttcctttgc 4080

T7_promoter ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gatga..... .......... .......... .......... .......... .......... 4085

4081 ctact..... .......... .......... .......... .......... .......... 4085

.......... .......... .......... .......... .......... ..........