e^?e^?LOCUS pIS2 3745 bp DNA SYN 28-Aug-2007 DEFINITION pIS2 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3745 /organism="pIS2" /mol_type="other DNA" misc_feature complement(85..105) /label="pBABE_3_primer" misc_feature complement(91..306) /label="SV40_enhancer" promoter 103..371 /label="SV40_promoter" rep_origin 270..347 /label="SV40_origin" promoter 223..425 /label="SV40_promoter" misc_feature 332..351 /label="SV40pro_F_primer" misc_feature 489..621 /label="chim_int" promoter 666..684 /label="T7_promoter" CDS 694..1629 /label="ORF frame 1" /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ*" misc_feature complement(1711..1730) /label="EBV_rev_primer" terminator complement(1697..1816) /label="SV40_PA_terminator" promoter 1989..2017 /label="AmpR_promoter" CDS 2059..2919 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 2059..2919 /label="Ampicillin" /gene="Ampicillin" rep_origin 3074..3693 /label="pBR322_origin" ORIGIN 1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct 61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca 481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga 661 gtacttaata cgactcacta taggctagcc accatgactt cgaaagttta tgatccagaa 721 caaaggaaac ggatgataac tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt 781 cttgattcat ttattaatta ttatgattca gaaaaacatg cagaaaatgc tgttattttt 841 ttacatggta acgcggcctc ttcttattta tggcgacatg ttgtgccaca tattgagcca 901 gtagcgcggt gtattatacc agaccttatt ggtatgggca aatcaggcaa atctggtaat 961 ggttcttata ggttacttga tcattacaaa tatcttactg catggtttga acttcttaat 1021 ttaccaaaga agatcatttt tgtcggccat gattggggtg cttgtttggc atttcattat 1081 agctatgagc atcaagataa gatcaaagca atagttcacg ctgaaagtgt agtagatgtg 1141 attgaatcat gggatgaatg gcctgatatt gaagaagata ttgcgttgat caaatctgaa 1201 gaaggagaaa aaatggtttt ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa 1261 atcatgagaa agttagaacc agaagaattt gcagcatatc ttgaaccatt caaagagaaa 1321 ggtgaagttc gtcgtccaac attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt 1381 aaacctgacg ttgtacaaat tgttaggaat tataatgctt atctacgtgc aagtgatgat 1441 ttaccaaaaa tgtttattga atcggaccca ggattctttt ccaatgctat tgttgaaggt 1501 gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa 1561 gatgcacctg atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat 1621 gaacaataat tctaggagct ctataccggt ctcgatatca ctactagtgt tctagagcgg 1681 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg 1741 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt 1801 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag 1861 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat 1921 aaggatccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 1981 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 2041 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 2101 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 2161 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 2221 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 2281 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 2341 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 2401 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 2461 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 2521 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 2581 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 2641 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 2701 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 2761 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 2821 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 2881 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 2941 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 3001 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3061 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3121 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 3181 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 3241 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 3301 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 3361 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 3421 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 3481 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 3541 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 3601 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 3661 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 3721 tggccttttg ctcacatggc tcgac //pIS2 "Times New Romane^?CUipBABE_3_primer"Times New Romane^?C[2 SV40_enhancer"Times New Romane^?CɆgs SV40_promoter"Times New Roman)e^?C SV40_promoter"Times New Roman>e^?[ SV40_origin"Times New RomanOe^?CL_SV40pro_F_primer"Times New Romane^?CɆmchim_int"Times New Romane^? T7_promoter"Times New Romane^?C] Orf frame 1"Times New Romane^?CɆSV40_PA_terminator"Times New Roman<e^?CEBV_rev_primer"Times New Romane^? AmpR_promoter"Times New Roman<e^?C g  Orf frame 1"Times New Roman<e^?C g  Ampicillin"Times New Romane^?CɆ m pBR322_origin"Times New Roman e^?BglII"Times New Roman)qeqQ4924@ e^?KpnI"Times New Roman:eQp>;/p> e^?PvuII"Times New RomanP"fR>w/> e^?StuI"Times New Roman#s#dYs e^?HindIII"Times New Roman  %%tk e^?PstI"Times New Roman &&[ e^?NheI"Times New RomanGYkk e^?BstBI"Times New Roman Kfoo e^?SSacI(1640), AgeI(1645), EcoRV(1656), SpeI(1663), XbaI(1671), NotI(1678), EagI(1678)"Times New Roman={/A> Qr e^?HpaI"Times New Roman >Rehet e^?BamHI(1923), ClaI(1916)"Times New Roman 7`>_RE`QEo e^?test"Times New Roman|'"