Sequence of pIS2

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Sequence of pIS2

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Sequence of pIS2 : 3745 BP; 1048 A; 803 C; 885 G; 1009 T; 0 other;

BglII(1) .......... .......... .......... .......... KpnI(58)

1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct 60

1 tctagacgcg tcgtggtacc ggactttatt ggagactttc tccttgaacc aatccatgga 60

.......... .......... .......... .......... .......... ..........

.......... PvuII(80) .......... .......... .......... ..........

61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 120

61 agactccgcc tttcttggtc gacaccttac acacagtcaa tcccacacct ttcaggggtc 120

.......... .......... .... pBABE_3_primer SV40_enhancer SV40_promoter

.......... .......... .......... .......... .......... ..........

121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 180

121 cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtccacac 180

.......... . SV40_enhancer SV40_promoter . .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 240

181 ctttcagggg tccgaggggt cgtccgtctt catacgtttc gtacgtagag ttaatcagtc 240

.......... . SV40_enhancer SV40_promoter . ........ SV40_promoter

.......... .......... .......... .......... .......... ..........

241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 300

241 gttggtatca gggcggggat tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg 300

... SV40_enhancer SV40_promoter SV40_promoter SV40_origin ...

.......... .......... .......... .......... .......... ..........

301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 360

301 taagaggcgg ggtaccgact gattaaaaaa aataaatacg tctccggctc cggcggagcc 360

.. SV40_promoter SV40_promoter SV40_origin SV40pro_F_primer ..

.......... .......... .......... ......... StuI(403) HindIII(420)

361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 420

361 ggagactcga taaggtcttc atcactcctc cgaaaaaacc tccggatccg aaaacgtttt 420

SV40_promoter .... SV40_promoter ........ .......... ..........

.......... .......... .......... .......... PstI(462) ..........

421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca 480

421 tcgaactaag aagactgtgt tgtcagagct tgaattcgac gtcttcaacc agcactccgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 540

481 gacccgtcca ttcatagttc caatgttctg tccaaattcc tctggttatc tttgacccga 540

.......... .......... .......... chim_int .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 600

541 acagctctgt ctcttctgag aacgcaaaga ctatccgtgg ataaccagaa tgactgtagg 600

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga 660

601 tgaaacggaa agagaggtgt ccacaggtga gggtcaagtt aatgtcgaga attccgatct 660

.......... chim_int .......... .......... .......... ..........

.......... .......... NheI(684) BstBI(700) .......... ..........

661 gtacttaata cgactcacta taggctagcc accatgactt cgaaagttta tgatccagaa 720

661 catgaattat gctgagtgat atccgatcgg tggtactgaa gctttcaaat actaggtctt 720

......... T7_promoter ......... ......... Orf frame 1 .........

.......... .......... .......... .......... .......... ..........

721 caaaggaaac ggatgataac tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt 780

721 gtttcctttg cctactattg accaggcgtc accacccggt ctacatttgt ttacttacaa 780

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cttgattcat ttattaatta ttatgattca gaaaaacatg cagaaaatgc tgttattttt 840

781 gaactaagta aataattaat aatactaagt ctttttgtac gtcttttacg acaataaaaa 840

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttacatggta acgcggcctc ttcttattta tggcgacatg ttgtgccaca tattgagcca 900

841 aatgtaccat tgcgccggag aagaataaat accgctgtac aacacggtgt ataactcggt 900

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtagcgcggt gtattatacc agaccttatt ggtatgggca aatcaggcaa atctggtaat 960

901 catcgcgcca cataatatgg tctggaataa ccatacccgt ttagtccgtt tagaccatta 960

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggttcttata ggttacttga tcattacaaa tatcttactg catggtttga acttcttaat 1020

961 ccaagaatat ccaatgaact agtaatgttt atagaatgac gtaccaaact tgaagaatta 1020

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttaccaaaga agatcatttt tgtcggccat gattggggtg cttgtttggc atttcattat 1080

1021 aatggtttct tctagtaaaa acagccggta ctaaccccac gaacaaaccg taaagtaata 1080

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 agctatgagc atcaagataa gatcaaagca atagttcacg ctgaaagtgt agtagatgtg 1140

1081 tcgatactcg tagttctatt ctagtttcgt tatcaagtgc gactttcaca tcatctacac 1140

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 attgaatcat gggatgaatg gcctgatatt gaagaagata ttgcgttgat caaatctgaa 1200

1141 taacttagta ccctacttac cggactataa cttcttctat aacgcaacta gtttagactt 1200

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gaaggagaaa aaatggtttt ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa 1260

1201 cttcctcttt tttaccaaaa cctcttattg aagaagcacc tttggtacaa cggtagtttt 1260

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atcatgagaa agttagaacc agaagaattt gcagcatatc ttgaaccatt caaagagaaa 1320

1261 tagtactctt tcaatcttgg tcttcttaaa cgtcgtatag aacttggtaa gtttctcttt 1320

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggtgaagttc gtcgtccaac attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt 1380

1321 ccacttcaag cagcaggttg taatagtacc ggagcacttt agggcaatca ttttccacca 1380

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aaacctgacg ttgtacaaat tgttaggaat tataatgctt atctacgtgc aagtgatgat 1440

1381 tttggactgc aacatgttta acaatcctta atattacgaa tagatgcacg ttcactacta 1440

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttaccaaaaa tgtttattga atcggaccca ggattctttt ccaatgctat tgttgaaggt 1500

1441 aatggttttt acaaataact tagcctgggt cctaagaaaa ggttacgata acaacttcca 1500

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa 1560

1501 cggttcttca aaggattatg acttaaacag tttcattttc cagaagtaaa aagcgttctt 1560

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gatgcacctg atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat 1620

1561 ctacgtggac tactttaccc ttttatatag tttagcaagc aactcgctca agagttttta 1620

.......... ......... Orf frame 1 ......... .......... ..........

.. SacI,AgeI EcoRV SpeI XbaI1) NotI(1678),EagI(1678)

1621 gaacaataat tctaggagct ctataccggt ctcgatatca ctactagtgt tctagagcgg 1680

1621 cttgttatta agatcctcga gatatggcca gagctatagt gatgatcaca agatctcgcc 1680

Orf frame 1 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg 1740

1681 ggcgaagctc gtctgtacta ttctatgtaa ctactcaaac ctgtttggtg ttgatcttac 1740

.......... ........ SV40_PA_terminator EBV_rev_primer ........

.......... .......... .......... .......... .......... ..........

1741 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt 1800

1741 gtcacttttt ttacgaaata aacactttaa acactacgat aacgaaataa acattggtaa 1800

.......... ...... SV40_PA_terminator ...... .......... ..........

.......... .......... HpaI(1821) .......... .......... ..........

1801 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag 1860

1801 tattcgacgt tatttgttca attgttgttg ttaacgtaag taaaatacaa agtccaagtc 1860

SV40_PA_terminator .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ClaI(1916)

1861 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat 1920

1861 cccctccaca ccctccaaaa aatttcgttc attttggaga tgtttacacc attttagcta 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aaggatccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 1980

1921 ttcctaggtc caccgtgaaa agccccttta cacgcgcctt ggggataaac aaataaaaag 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 2040

1981 atttatgtaa gtttatacat aggcgagtac tctgttattg ggactattta cgaagttatt 2040

.......... ........ AmpR_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 2100

2041 ataacttttt ccttctcata ctcataagtt gtaaaggcac agcgggaata agggaaaaaa 2100

.......... .......... .... Orf frame 1 Ampicillin .... ..........

.......... .......... .......... .......... .......... ..........

2101 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 2160

2101 cgccgtaaaa cggaaggaca aaaacgagtg ggtctttgcg accactttca ttttctacga 2160

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 2220

2161 cttctagtca acccacgtgc tcacccaatg tagcttgacc tagagttgtc gccattctag 2220

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 2280

2221 gaactctcaa aagcggggct tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat 2280

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 2340

2281 acaccgcgcc ataatagggc ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg 2340

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 2400

2341 ataagagtct tactgaacca actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg 2400

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 2460

2401 tactgtcatt ctcttaatac gtcacgacgg tattggtact cactattgtg acgccggttg 2460

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 2520

2461 aatgaagact gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc 2520

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 2580

2521 ctagtacatt gagcggaact agcaaccctt ggcctcgact tacttcggta tggtttgctg 2580

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 2640

2581 ctcgcactgt ggtgctacgg acatcgttac cgttgttgca acgcgtttga taattgaccg 2640

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 2700

2641 cttgatgaat gagatcgaag ggccgttgtt aattatctga cctacctccg cctatttcaa 2700

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 2760

2701 cgtcctggtg aagacgcgag ccgggaaggc cgaccgacca aataacgact atttagacct 2760

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 2820

2761 cggccactcg cacccagagc gccatagtaa cgtcgtgacc ccggtctacc attcgggagg 2820

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 2880

2821 gcatagcatc aatagatgtg ctgcccctca gtccgttgat acctacttgc tttatctgtc 2880

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 2940

2881 tagcgactct atccacggag tgactaattc gtaaccattg acagtctggt tcaaatgagt 2940

.... Orf frame 1 Ampicillin .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 3000

2941 atatatgaaa tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3060

3001 gaaaaactat tagagtactg gttttaggga attgcactca aaagcaaggt gactcgcagt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3120

3061 ctggggcatc ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg 3120

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3121 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 3180

3121 acgaacgttt gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 3240

3181 ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaagaa 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 3300

3241 gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 3360

3301 cgagacgatt aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 3420

3361 aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 3480

3421 acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 3540

3481 gatactcttt cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 3600

3541 tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 3660

3601 tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta aaaacactac gagcagtccc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 3720

3661 cccgcctcgg ataccttttt gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg 3720

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tggccttttg ctcacatggc tcgac..... .......... .......... .......... 3745

3721 accggaaaac gagtgtaccg agctg..... .......... .......... .......... 3745

.......... .......... .......... .......... .......... ..........