Sequence of pIZ

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Sequence of pIZ_V5-His

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Sequence of pIZ/V5-His : 2876 BP; 691 A; 782 C; 697 G; 706 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ggatcatgat gataaacaat gtatggtgct aatgttgctt caacaacaat tctgttgaac 60

1 cctagtacta ctatttgtta cataccacga ttacaacgaa gttgttgtta agacaacttg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tgtgttttca tgtttgccaa caagcacctt tatactcggt ggcctcccca ccaccaactt 120

61 acacaaaagt acaaacggtt gttcgtggaa atatgagcca ccggaggggt ggtggttgaa 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttttgcactg caaaaaaaca cgcttttgca cgcgggccca tacatagtac aaactctacg 180

121 aaaacgtgac gtttttttgt gcgaaaacgt gcgcccgggt atgtatcatg tttgagatgc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tttcgtagac tattttacat aaatagtcta caccgttgta tacgctccaa atacactacc 240

181 aaagcatctg ataaaatgta tttatcagat gtggcaacat atgcgaggtt tatgtgatgg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 acacattgaa cctttttgca gtgcaaaaaa gtacgtgtcg gcagtcacgt aggccggcct 300

241 tgtgtaactt ggaaaaacgt cacgtttttt catgcacagc cgtcagtgca tccggccgga 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tatcgggtcg cgtcctgtca cgtacgaatc acattatcgg accggacgag tgttgtctta 360

301 atagcccagc gcaggacagt gcatgcttag tgtaatagcc tggcctgctc acaacagaat 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcgtgacagg acgccagctt cctgtgttgc taaccgcagc cggacgcaac tccttatcgg 420

361 agcactgtcc tgcggtcgaa ggacacaacg attggcgtcg gcctgcgttg aggaatagcc 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aacaggacgc gcctccatat cagccgcgcg ttatctcatg cgcgtgaccg gacacgaggc 480

421 ttgtcctgcg cggaggtata gtcggcgcgc aatagagtac gcgcactggc ctgtgctccg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcccgtcccg cttatcgcgc ctataaatac agcccgcaac gatctggtaa acacagttga 540

481 cgggcagggc gaatagcgcg gatatttatg tcgggcgttg ctagaccatt tgtgtcaact 540

.......... .......... .......... ........ OpIE2_fwd_pri ........

......... HindIII(561) KpnI(571) SacI(577),BamHI(579) .........

541 acagcatctg ttcgaattta aagcttggta ccgagctcgg atccactagt ccagtgtggt 600

541 tgtcgtagac aagcttaaat ttcgaaccat ggctcgagcc taggtgatca ggtcacacca 600

.......... .......... .......... .......... .......... ..........

EcoRI02) EcoRV(614) NotI(629) XhoI(635) XbaI(641) ..........

601 ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg 660

601 ccttaagacg tctataggtc gtgtcaccgc cggcgagctc agatctcccg ggcgccaagc 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... AgeI(708) ..........

661 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc 720

661 ttccattcgg atagggattg ggagaggagc cagagctaag atgcgcatgg ccagtagtag 720

.......... .......... V5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 accatcacca ttgagtttat ctgactaaat cttagtttgt attgtcatgt tttaatacaa 780

721 tggtagtggt aactcaaata gactgattta gaatcaaaca taacagtaca aaattatgtt 780

6xHis .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tatgttatgt ttaaatatgt ttttaataaa ttttataaaa taatttcaac ttttattgta 840

781 atacaataca aatttataca aaaattattt aaaatatttt attaaagttg aaaataacat 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ClaI(882) ..........

841 acaacattgt ccatttacac actcctttca agcgcgtggg atcgatgctc actcaaaggc 900

841 tgttgtaaca ggtaaatgtg tgaggaaagt tcgcgcaccc tagctacgag tgagtttccg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 960

901 ccattatgcc aataggtgtc ttagtcccct attgcgtcct ttcttgtaca ctcgttttcc 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 1020

961 ggtcgttttc cggtccttgg catttttccg gcgcaacgac cgcaaaaagg tatccgaggc 1020

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 1080

1021 ggggggactg ctcgtagtgt ttttagctgc gagttcagtc tccaccgctt tgggctgtcc 1080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 1140

1081 tgatatttct atggtccgca aagggggacc ttcgagggag cacgcgagag gacaaggctg 1140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 1200

1141 ggacggcgaa tggcctatgg acaggcggaa agagggaagc ccttcgcacc gcgaaagagt 1200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 1260

1201 atcgagtgcg acatccatag agtcaagcca catccagcaa gcgaggttcg acccgacaca 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 1320

1261 cgtgcttggg gggcaagtcg ggctggcgac gcggaatagg ccattgatag cagaactcag 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 1380

1321 gttgggccat tctgtgctga atagcggtga ccgtcgtcgg tgaccattgt cctaatcgtc 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 1440

1381 tcgctccata catccgccac gatgtctcaa gaacttcacc accggattga tgccgatgtg 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 1500

1441 atcttcttgt cataaaccat agacgcgaga cgacttcggt caatggaagc ctttttctca 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 1560

1501 accatcgaga actaggccgt ttgtttggtg gcgaccatcg ccaccaaaaa aacaaacgtt 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 1620

1561 cgtcgtctaa tgcgcgtctt tttttcctag agttcttcta ggaaactaga aaagatgccc 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga tgataaacaa 1680

1621 cagactgcga gtcaccttgc ttttgagtgc aattccctaa aaccagtact actatttgtt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgtatggtgc taatgttgct tcaacaacaa ttctgttgaa ctgtgttttc atgtttgcca 1740

1681 acataccacg attacaacga agttgttgtt aagacaactt gacacaaaag tacaaacggt 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 acaagcacct ttatactcgg tggcctcccc accaccaact tttttgcact gcaaaaaaac 1800

1741 tgttcgtgga aatatgagcc accggagggg tggtggttga aaaaacgtga cgtttttttg 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 acgcttttgc acgcgggccc atacatagta caaactctac gtttcgtaga ctattttaca 1860

1801 tgcgaaaacg tgcgcccggg tatgtatcat gtttgagatg caaagcatct gataaaatgt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 taaatagtct acaccgttgt atacgctcca aatacactac cacacattga acctttttgc 1920

1861 atttatcaga tgtggcaaca tatgcgaggt ttatgtgatg gtgtgtaact tggaaaaacg 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agtgcaaaaa agtacgtgtc ggcagtcacg taggccggcc ttatcgggtc gcgtcctgtc 1980

1921 tcacgttttt tcatgcacag ccgtcagtgc atccggccgg aatagcccag cgcaggacag 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 acgtacgaat cacattatcg gaccggacga gtgttgtctt atcgtgacag gacgccagct 2040

1981 tgcatgctta gtgtaatagc ctggcctgct cacaacagaa tagcactgtc ctgcggtcga 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcctgtgttg ctaaccgcag ccggacgcaa ctccttatcg gaacaggacg cgcctccata 2100

2041 aggacacaac gattggcgtc ggcctgcgtt gaggaatagc cttgtcctgc gcggaggtat 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tcagccgcgc gttatctcat gcgcgtgacc ggacacgagg cgcccgtccc gcttatcgcg 2160

2101 agtcggcgcg caatagagta cgcgcactgg cctgtgctcc gcgggcaggg cgaatagcgc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cctataaata cagcccgcaa cgatctggta aacacagttg aacagcatct gttcgaatta 2220

2161 ggatatttat gtcgggcgtt gctagaccat ttgtgtcaac ttgtcgtaga caagcttaat 2220

.......... ........ OpIE2_fwd_pri ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 attcggatct ctgcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta 2280

2221 taagcctaga gacgtcgtgc acaactgtta attagtagcc gtatcatata gccgtatcat 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 taatacgact cactatagga gggccaccat ggccaagttg accagtgccg < font style = "background-color:#8600C9">ttccggtgct 2340

2281 attatgctga gtgatatcct cccggtggta ccggttcaac tggtcacggc aaggccacga 2340

T7_pro .......... .......... ......... bleo sh_ble .........

......... AatII(2355) ......... .......... .......... SmaI(2399)

2341 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg 2400

2341 gtggcgcgcg ctgcagcggc ctcgccagct caagacctgg ctggccgagc ccaagagggc 2400

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag 2460

2401 cctgaagcac ctcctgctga agcggccaca ccaggccctg ctgcactggg acaagtagtc 2460

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct 2520

2461 gcgccaggtc ctggtccacc acggcctgtt gtgggaccgg acccacaccc acgcgccgga 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg 2580

2521 cctgctcgac atgcggctca ccagcctcca gcacaggtgc ttgaaggccc tgcggaggcc 2580

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc 2640

2581 cggccggtac tggctctagc cgctcgtcgg cacccccgcc ctcaagcggg acgcgctggg 2640

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgaccgacgc cgaccaacac 2700

2641 ccggccgttg acgcacgtga agcaccggct cctcgtcctg actggctgcg gctggttgtg 2700

.......... bleo sh_ble .......... .......... ..........

.......... .......... .......... SalI(2736) .......... ..........

2701 cgccggtccg acggcggccc acgggtccca ggggggtcga cctcgaaact tgtttattgc 2760

2701 gcggccaggc tgccgccggg tgcccagggt ccccccagct ggagctttga acaaataacg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 2820

2761 tcgaatatta ccaatgttta tttcgttatc gtagtgttta aagtgtttat ttcgtaaaaa 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtct.... 2876

2821 aagtgacgta agatcaacac caaacaggtt tgagtagtta catagaatag tacaga.... 2876

.......... ......... EBV_rev_pri ......... .......... ..........