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Backbone size: 5464
Bacteria Resistance: Ampicillin
Catalog Number: 37074
GenBank Accession Number:X98612
Comments:Restriction digests analyzed on agarose gels give the following sizes (kb): HindIII--5.4; BamHI--5.4; PstI/HindIII--4.6, 0.8; PstI/SalI--3.3, 2.3. (ATCC staff) A cosmid vector containing a single cos site.  Cloning into the BamHI or EcoRI site involves 2 preparations of dephosphorylated vector, one linearized with HindIII, the other linearized with SalI.  An equimolar ratio of right-hand and left-hand cos containing vector fragments, digested with BamHI or EcoRI can be ligated to appropriately digested and dephosphorylated inserts.  Only molecules with 2 cos sites separated by 37-50 kb can be packaged.  Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli. Related vectors: Charon 4, pAT153. (Information source: VectorDB.)