Sequence of pJL43.1

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pJL43.1

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pJL43.1 - Pglh-2::MosTase::glh-2utr : 7077 BP; 1990 A; 1492 C; 1528 G; 2067 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 60

1 caccgtgaaa agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 120

61 gtttatacat aggcgagtac tctgttattg ggactattta cgaagttatt ataacttttt 120

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 180

121 ccttctcata ctcataagtt gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa 180

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 240

181 cggaaggaca aaaacgagtg ggtctttgcg accactttca ttttctacga cttctagtca 240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 300

241 acccacgtgc tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa 300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 360

301 aagcggggct tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc 360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 420

361 ataatagggc ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg ataagagtct 420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 480

421 tactgaacca actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt 480

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 540

481 ctcttaatac gtcacgacgg tattggtact cactattgtg acgccggttg aatgaagact 540

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 600

541 gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt 600

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 660

601 gagcggaact agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt 660

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 720

661 ggtgctacgg acatcgttac cgttgttgca acgcgtttga taattgaccg cttgatgaat 720

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 780

721 gagatcgaag ggccgttgtt aattatctga cctacctccg cctatttcaa cgtcctggtg 780

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 840

781 aagacgcgag ccgggaaggc cgaccgacca aataacgact atttagacct cggccactcg 840

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 900

841 cacccagagc gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc 900

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 960

901 aatagatgtg ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct 960

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1020

961 atccacggag tgactaattc gtaaccattg acagtctggt tcaaatgagt atatatgaaa 1020

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1080

1021 tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag gaaaaactat 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1140

1081 tagagtactg gttttaggga attgcactca aaagcaaggt gactcgcagt ctggggcatc 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1200

1141 ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt 1200

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1260

1201 gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1320

1261 aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaggaa gatcacatcg 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1380

1321 gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1440

1381 aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1500

1441 ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1560

1501 ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc gatactcttt 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1620

1561 cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1680

1621 gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata tcaggacagc 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1740

1681 ccaaagcggt ggagactgaa ctcgcagcta aaaacactac gagcagtccc cccgcctcgg 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1800

1741 ataccttttt gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac 1800

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1860

1801 gagtgtacaa gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1920

1861 tcactcgact atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 1980

1921 ttcgccttct cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 2040

1981 cgtcgaccgt gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattac 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 2100

2041 actcaatcga gtgagtaatc cgtggggtcc gaaatgtgaa atacgaaggc cgagcataca 2100

.......... .......... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 2160

2101 acacacctta acactcgcct attgttaaag tgtgtccttt gtcgatactg gtactaatgc 2160

.......... .......... M13_pUC_r .......... M13_r ..........

.......... .......... .......... .... KpnI(2206) ApaI(2212),XhoI(2217)

2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg 2220

2161 ggttcgcgcg ttaattggga gtgatttccc ttgttttcga cccatggccc gggggggagc 2220

.......... T3 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aggtcgacgg tatcgataag cttgatatcg aattcatatt accattattc ttgaactttt 2280

2221 tccagctgcc atagctattc gaactatagc ttaagtataa tggtaataag aacttgaaaa 2280

pBlueKS_pri ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 atagtctttg gtagtggttt cgtgtagaaa ggcattggag tcgaaaggtt tgaaagtggt 2340

2281 tatcagaaac catcaccaaa gcacatcttt ccgtaacctc agctttccaa actttcacca 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcagaagaat ccgagtcgaa atgaatccca gaatctacaa aaacttttat taaacaattt 2400

2341 agtcttctta ggctcagctt tacttagggt cttagatgtt tttgaaaata atttgttaaa 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 agagcttttg agagtgtcat gttaaatttg cattcataac tgttttgtta tctgtttcta 2460

2401 tctcgaaaac tctcacagta caatttaaac gtaagtattg acaaaacaat agacaaagat 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctaattagtt ccaccgaatc aggatgcagt gagcgaaacc gaaatcgtga atttcggttt 2520

2461 gattaatcaa ggtggcttag tcctacgtca ctcgctttgg ctttagcact taaagccaaa 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgcccacagg ggacgaaaag taggttacta atatgtgaag cttttaaaaa ctccataaat 2580

2521 gcgggtgtcc cctgcttttc atccaatgat tatacacttc gaaaattttt gaggtattta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tttgtgaact ccacagctag gatcaagggt gccgatttat cgtttttcag gaaaacattt 2640

2581 aaacacttga ggtgtcgatc ctagttccca cggctaaata gcaaaaagtc cttttgtaaa 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ttttgtttac atttttttaa aaagttgcgg gttttctcgt tttagaataa attcatgttt 2700

2641 aaaacaaatg taaaaaaatt tttcaacgcc caaaagagca aaatcttatt taagtacaaa 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccttaatttt tttttcatca acacaaaaaa ttgattatta aacattttct taaaaaattt 2760

2701 ggaattaaaa aaaaagtagt tgtgtttttt aactaataat ttgtaaaaga attttttaaa 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tccgattttt cgtttttcag gaaaatcgaa attttttcgc ttttccgata tttcgaaaaa 2820

2761 aggctaaaaa gcaaaaagtc cttttagctt taaaaaagcg aaaaggctat aaagcttttt 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tatttcattt ttagagttta tcttcttttt agaagaaaat tatgttttct tatgattttt 2880

2821 ataaagtaaa aatctcaaat agaagaaaaa tcttctttta atacaaaaga atactaaaaa 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... HpaI(2925) ..........

2881 cccatcaaca aaacaataat tttttgaaac tgatttttct ttgttaactt tctaaatttt 2940

2881 gggtagttgt tttgttatta aaaaactttg actaaaaaga aacaattgaa agatttaaaa 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tttatttttc aggaaaatcg aacaatcgaa aattgttcat ttttcatgat accctaaaaa 3000

2941 aaataaaaag tccttttagc ttgttagctt ttaacaagta aaaagtacta tgggattttt 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tataaagttt gatcgatata atctatttca atcttatgaa aatttttaaa ataaactgac 3060

3001 atatttcaaa ctagctatat tagataaagt tagaatactt ttaaaaattt tatttgactg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cagaatcgga tgttgcttcg gtagctgaga ggttccattg ttcttcctca tcgacttcat 3120

3061 gtcttagcct acaacgaagc catcgactct ccaaggtaac aagaaggagt agctgaagta 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 catcactatc ttcttcaatc gttgttacta cccattgttt tccaaccaat ttttcattgc 3180

3121 gtagtgatag aagaagttag caacaatgat gggtaacaaa aggttggtta aaaagtaacg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 acttgaaatt ttcaagggct ttcatactac tttgatacat ttccaatgcc gaattatgct 3240

3181 tgaactttaa aagttcccga aagtatgatg aaactatgta aaggttacgg cttaatacga 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cgttagtcat caaatcctca gcaggagaaa caccatccga gaaggaatac tttccaaaac 3300

3241 gcaatcagta gtttaggagt cgtcctcttt gtggtaggct cttccttatg aaaggttttg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 caacagagaa taaactttga ttttccatat caaatccaga agacttgtag tttgatattg 3360

3301 gttgtctctt atttgaaact aaaaggtata gtttaggtct tctgaacatc aaactataac 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gtctagaaat ctggctgcag cgagtttcag agttacagcg agcaaaatca tattgtatca 3420

3361 cagatcttta gaccgacgtc gctcaaagtc tcaatgtcgc tcgttttagt ataacatagt 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gagccgtgaa gtacgtgaat ttcttgtatg aaaccagttt ttgaagctgt ttgagaatga 3480

3421 ctcggcactt catgcactta aagaacatac tttggtcaaa aacttcgaca aactcttact 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aaatatgttc atcgcaaggt ttttcagcat actttgaagc gtcacgtggg ttcagaatga 3540

3481 tttatacaag tagcgttcca aaaagtcgta tgaaacttcg cagtgcaccc aagtcttact 3540

.......... .......... .......... .......... .......... ..........

......... BglII(3556) ......... .......... .......... ..........

3541 gttttccgtc cggtaagatc tcaatcaacg tttcagtgtt aagctctggt tgtgtgacat 3600

3541 caaaaggcag gccattctag agttagttgc aaagtcacaa ttcgagacca acacactgta 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 actgcaattt tttaattgaa ggcaaaattt gatgttcaaa caaatttacg ttgaaaatcg 3660

3601 tgacgttaaa aaattaactt ccgttttaaa ctacaagttt gtttaaatgc aacttttagc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ccgtccgaat cgcgtatact tcgttaaaat gaacgttgaa tgaccgaagg gattgaagaa 3720

3661 ggcaggctta gcgcatatga agcaatttta cttgcaactt actggcttcc ctaacttctt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tacaagctaa aacgtcgttg gcagtattcg tgatatgaac ccttaagaat attgtttaaa 3780

3721 atgttcgatt ttgcagcaac cgtcataagc actatacttg ggaattctta taacaaattt 3780

.......... .......... .......... .......... .......... ..........

MscI(3785) .......... .......... .......... .......... ..........

3781 tttggccaaa ttcagagtta ttaccattga atcagaagtt gaataatcca tgatttttct 3840

3781 aaaccggttt aagtctcaat aatggtaact tagtcttcaa cttattaggt actaaaaaga 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tccataggca acggttctgc gaccattgca atcattgatt ggcatgaaat gtgtgccata 3900

3841 aggtatccgt tgccaagacg ctggtaacgt tagtaactaa ccgtacttta cacacggtat 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcgtcgtcct tcattgtatg gcttctcagt gactcgataa tttttttcgc gaggggttcg 3960

3901 agcagcagga agtaacatac cgaagagtca ctgagctatt aaaaaaagcg ctccccaagc 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 attccatcct tgctttgata atactgagtt atgtggctga gaagaactcc tcctcctcgc 4020

3961 taaggtagga acgaaactat tatgactcaa tacaccgact cttcttgagg aggaggagcg 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggacgaggca aatagtcttg cattgaaagt tcgttgagag catgaattgc gtccagtaca 4080

4021 cctgctccgt ttatcagaac gtaactttca agcaactctc gtacttaacg caggtcatgt 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctaatattca tttctgaaaa aattaattaa attgtaaatt aaatatgcca aaagataaac 4140

4081 gattataagt aaagactttt ttaattaatt taacatttaa tttatacggt tttctatttg 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gaacgtttaa gtttaaaaag gtctccagat taattaggga aactattttc agaaagaaaa 4200

4141 cttgcaaatt caaatttttc cagaggtcta attaatccct ttgataaaag tctttctttt 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 attctgaagg ttccacaaag tattactggt atggcaaaaa gtgcgatgca aggatactga 4260

4201 taagacttcc aaggtgtttc ataatgacca taccgttttt cacgctacgt tcctatgact 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aattgtattc agaaaatccc aaattgagtt tttaaaaggc tcatacgagc agcataggac 4320

4261 ttaacataag tcttttaggg tttaactcaa aaattttccg agtatgctcg tcgtatcctg 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aagggtctcg ccacgttcgt cctctacagt actcccgcgc ggcaaatttc aaatcttttg 4380

4321 ttcccagagc ggtgcaagca ggagatgtca tgagggcgcg ccgtttaaag tttagaaaac 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgcgcgagtt tctgagtttc cattcattta aaattttttg gtttgcaaac gccaaacttg 4440

4381 gcgcgctcaa agactcaaag gtaagtaaat tttaaaaaac caaacgtttg cggtttgaac 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tactttttcc tggaagttcg aagacgcgtt atgtcgagtt tcgtgccgaa taaagagcaa 4500

4441 atgaaaaagg accttcaagc ttctgcgcaa tacagctcaa agcacggctt atttctcgtt 4500

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

4501 acgcggacag tattaatttt ctgttttcat ttgaagaaaa cagctgcgga atcgcaccga 4560

4501 tgcgcctgtc ataattaaaa gacaaaagta aacttctttt gtcgacgcct tagcgtggct 4560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 atgcttgttg aagcctttgg cgaacaagta ccaactgtga aaacgtgtga acggtggttt 4620

4561 tacgaacaac ttcggaaacc gcttgttcat ggttgacact tttgcacact tgccaccaaa 4620

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 caacgcttca aaagtggtga ttttgacgtc gacgacaaag agcacggaaa accgccaaaa 4680

4621 gttgcgaagt tttcaccact aaaactgcag ctgctgtttc tcgtgccttt tggcggtttt 4680

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aggtacgaag acgccgaact gcaagcatta ttggatgaag acgatgctca aacgtaagtt 4740

4681 tccatgcttc tgcggcttga cgttcgtaat aacctacttc tgctacgagt ttgcattcaa 4740

.......... .......... ORF 1 .......... .......... ..........

.......... .......... NcoI(4762) .......... .......... ..........

4741 taaacatata tactaactaa cccatggatt atttaaattt tcaggcaaaa acaactcgca 4800

4741 atttgtatat atgattgatt gggtacctaa taaatttaaa agtccgtttt tgttgagcgt 4800

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gagcagttgg aagtaagtca acaagcagtt tccaatcgct tgcgagagat gggaaagatt 4860

4801 ctcgtcaacc ttcattcagt tgttcgtcaa aggttagcga acgctctcta ccctttctaa 4860

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cagaaggtcg gtagatgggt gccacatgag ttgaacgaga ggcagatgga gaggcgcaaa 4920

4861 gtcttccagc catctaccca cggtgtactc aacttgctct ccgtctacct ctccgcgttt 4920

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aacacatgcg aaattttgct ttcacgatac aaaaggaagt cgtttttgca tcgtatcgtt 4980

4921 ttgtgtacgc tttaaaacga aagtgctatg ttttccttca gcaaaaacgt agcatagcaa 4980

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 actggagatg aaaaatggat cttttttgtt aatcctaaac gtaaaaagtc atacgttgat 5040

4981 tgacctctac tttttaccta gaaaaaacaa ttaggatttg catttttcag tatgcaacta 5040

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cctggacaac cggccacatc gactgctcga ccgaatcgct ttggcaagaa gacgatgctc 5100

5041 ggacctgttg gccggtgtag ctgacgagct ggcttagcga aaccgttctt ctgctacgag 5100

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tgtgtttggt gggatcagag cggtgtcatt tactatgagc tcttgaaacc cggcgaaacg 5160

5101 acacaaacca ccctagtctc gccacagtaa atgatactcg agaactttgg gccgctttgc 5160

.......... .......... ORF 1 .......... .......... ..........

.......... .......... BclI(5189) .......... .......... ..........

5161 gtgaatacgg cacgctacca acaacaattg atcaatttga accgtgcgct tcagagaaaa 5220

5161 cacttatgcc gtgcgatggt tgttgttaac tagttaaact tggcacgcga agtctctttt 5220

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cgaccggaat atcaaaaaag acaacacagg gtcatttttc tccatgacaa cgctccatca 5280

5221 gctggcctta tagttttttc tgttgtgtcc cagtaaaaag aggtactgtt gcgaggtagt 5280

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 catacggcaa gagcggttcg cgacacgttg gaaacactca attgggaagt gcttccgcat 5340

5281 gtatgccgtt ctcgccaagc gctgtgcaac ctttgtgagt taacccttca cgaaggcgta 5340

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcggcttact caccagacct ggccccatcc gattaccacc tattcgcttc gatgggacac 5400

5341 cgccgaatga gtggtctgga ccggggtagg ctaatggtgg ataagcgaag ctaccctgtg 5400

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gcactcgctg agcagcgctt cgattcttac gaaagtgtga aaaaatggct cgatgaatgg 5460

5401 cgtgagcgac tcgtcgcgaa gctaagaatg ctttcacact tttttaccga gctacttacc 5460

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ttcgccgcaa aagacgatga gttctactgg cgtggaatcc acaaattgcc cgagagatgg 5520

5461 aagcggcgtt ttctgctact caagatgacc gcaccttagg tgtttaacgg gctctctacc 5520

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... NheI(5559) .......... ..........

5521 gaaaaatgtg tcgcgagcga cggcaaatac tttgaatagc tagcatgtat ctgcatatcc 5580

5521 ctttttacac agcgctcgct gccgtttatg aaacttatcg atcgtacata gacgtatagg 5580

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ttcaaatgtt gtccatattg tatcagtaaa ttataatgcc ccctttttat atattctcct 5640

5581 aagtttacaa caggtataac atagtcattt aatattacgg gggaaaaata tataagagga 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tatcatgtaa atgttctatt gattttttgc tggtgtaaaa cgtttttata gtaaatcctc 5700

5641 atagtacatt tacaagataa ctaaaaaacg accacatttt gcaaaaatat catttaggag 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tccacagtga atttgttaaa ttgtatcaaa acaagacatt gattaaattt gttcgagact 5760

5701 aggtgtcact taaacaattt aacatagttt tgttctgtaa ctaatttaaa caagctctga 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tgtggattgt aaaatatttt gcagaaaatg gcaatattca tttagaaaca ttacttccat 5820

5761 acacctaaca ttttataaaa cgtcttttac cgttataagt aaatctttgt aatgaaggta 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 aataggaaaa actatttatg agttttcatg tgcctatgca agttcccact ctgtgtgaat 5880

5821 ttatcctttt tgataaatac tcaaaagtac acggatacgt tcaagggtga gacacactta 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ttcatcatac aaattgagca aacataaggt ttatgtccag tatgggttct cagatgtttt 5940

5881 aagtagtatg tttaactcgt ttgtattcca aatacaggtc atacccaaga gtctacaaaa 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gtaagagttg agctgtcggt gaatgccttc tcacattgag cacatggata cggtctctct 6000

5941 cattctcaac tcgacagcca cttacggaag agtgtaactc gtgtacctat gccagagaga 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ccagtgtgtg ttctgaaata atgaaaaata gtcttgcaat atgtttctac tattcgagaa 6060

6001 ggtcacacac aagactttat tactttttat cagaacgtta tacaaagatg ataagctctt 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 aaaatgtaat tttttttctt atggtaacct gcaatttata tattttttat ttgccgttat 6120

6061 ttttacatta aaaaaaagaa taccattgga cgttaaatat ataaaaaata aacggcaata 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tgacattatt ataagaaatt ggaggatatc acaaatatcg taaattctcc aaaattttga 6180

6121 actgtaataa tattctttaa cctcctatag tgtttatagc atttaagagg ttttaaaact 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 cacttcactt taaagacaaa tgcggttttt ccgagtgtgt ctcgccacga tctcagtcat 6240

6181 gtgaagtgaa atttctgttt acgccaaaaa ggctcacaca gagcggtgct agagtcagta 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 aatggtcgct acaggtgcca aactttaggc atttgttata tttgatgcag aaaaccaagc 6300

6241 ttaccagcga tgtccacggt ttgaaatccg taaacaatat aaactacgtc ttttggttcg 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 acatgaaaat taaaattgtc aagtagacaa tgaaatccag acattgttat gattcatttt 6360

6301 tgtactttta attttaacag ttcatctgtt actttaggtc tgtaacaata ctaagtaaaa 6360

.......... .......... .......... .......... .......... ..........

. SmaI,BamHI SpeI NotI(6403),EagI(6403) SacII(6415)

6361 tttgcgaatt cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg 6420

6361 aaacgcttaa ggacgtcggg ccccctaggt gatcaagatc tcgccggcgg tggcgccacc 6420

.......... .......... ......... pBlueSK_pri ......... ..........

.......... .......... .......... .......... .......... ..........

6421 agctccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa 6480

6421 tcgaggttaa gcgggatatc actcagcata atgcgcgcga gtgaccggca gcaaaatgtt 6480

.......... .......... T7_pro ....... M13_f M13_pUC_f lacZ_a

.......... .......... .......... .......... .......... ..........

6481 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 6540

6481 gcagcactga cccttttggg accgcaatgg gttgaattag cggaacgtcg tgtaggggga 6540

M13_pUC_f .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 6600

6541 aagcggtcga ccgcattatc gcttctccgg gcgtggctag cgggaagggt tgtcaacgcg 6600

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 6660

6601 tcggacttac cgcttaccct gcgcgggaca tcgccgcgta attcgcgccg cccacaccac 6660

lacZ_a .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

6661 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 6720

6661 caatgcgcgt cgcactggcg atgtgaacgg tcgcgggatc gcgggcgagg aaagcgaaag 6720

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 6780

6721 aagggaagga aagagcggtg caagcggccg aaaggggcag ttcgagattt agcccccgag 6780

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 6840

6781 ggaaatccca aggctaaatc acgaaatgcc gtggagctgg ggttttttga actaatccca 6840

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 6900

6841 ctaccaagtg catcacccgg tagcgggact atctgccaaa aagcgggaaa ctgcaacctc 6900

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 6960

6901 aggtgcaaga aattatcacc tgagaacaag gtttgacctt gttgtgagtt gggatagagc 6960

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 7020

6961 cagataagaa aactaaatat tccctaaaac ggctaaagcc ggataaccaa ttttttactc 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttag... 7077

7021 gactaaattg tttttaaatt gcgcttaaaa ttgttttata attgcgaatg ttaaatc... 7077

.......... .......... .......... .......... .......... ..........