Download the plasmid map
Use BVTech Plasmid to view and edit this plasmid map.
Dowload BVTech Plasmid
If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.
BVTech Plasmid is DNA sequence analysis and plasmid drawing software for Windows PCs.
You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your
DNA sequencing data, align sequences, and much more.
Free to try, $39.98 to buy
Payments will be processed by PayPal. PayPal is the safer, easier way to pay.
View the sequence with features on line
pJL44 - Phsp16.48::MosTase::glh-2utr
Gene/insert name: Phsp-16::Mos1 transposase
Insert size (bp): 2279
Species of gene(s): C. elegans (nematode)
Vector backbone: N/A (Search Vector Database)
Type of vector: Worm expression
Backbone size (bp): 4809
5' Sequencing primer: n/a (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Erik Jorgensen
Comments: This plasmid was made by Dr. Jean Louis Bessereau and described in the publication: Bessereau JL, Wright A, Williams DC, Schuske K, Davis MW, Jorgensen EM. Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line. Nature. 2001 Sep 6;413(6851):70-4. There is che-1 sequence in the plasmid. It comes from the glh-2 3' UTR fragment in the plasmid. The UTR fragment extends into the 3' region of the che-1 gene, which is tail to tail with glh-2 on the chromosome. This is only the last exon of che-1 and it is not relevant to the function of the construct.
Article: Single-copy insertion of transgenes in Caenorhabditis elegans Christian Frøkjær-Jensen et al. ( Pubmed)