Sequence of pJL44

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Sequence of pJL44

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Sequence of pJL44 - Phsp16.48::MosTase::glh-2utr : 4809 BP; 1328 A; 1043 C; 1139 G; 1299 T; 0 other;

......... HindIII(18) ......... .......... .......... ..........

1 atgaccatga ttacgccaag cttgctggac ggaaatagtg gtaaagtgac atgattgtag 60

1 tactggtact aatgcggttc gaacgacctg cctttatcac catttcactg tactaacatc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tttgaagatt tcacaattag agtgaatgtt gtttggttcg gttttgtcac tgtatttata 120

61 aaacttctaa agtgttaatc tcacttacaa caaaccaagc caaaacagtg acataaatat 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ctcatttcca cctttttcta gaacattcga gctgcttctt gcaaaaggag ggcgactcac 180

121 gagtaaaggt ggaaaaagat cttgtaagct cgacgaagaa cgttttcctc ccgctgagtg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 attcagaaca ttgagaaata gtgtgcgtac tgaagaaacc cagatacttt ttcaatctgc 240

181 taagtcttgt aactctttat cacacgcatg acttctttgg gtctatgaaa aagttagacg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gtctctttgc acctatgggg tgtattttga aatgaatgca tctaggacct tctagaacat 300

241 cagagaaacg tggatacccc acataaaact ttacttacgt agatcctgga agatcttgta 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tctaaacggc tgcaggatac gggtatataa gccaatcgtg ttcagaggaa accaatacac 360

301 agatttgccg acgtcctatg cccatatatt cggttagcac aagtctcctt tggttatgtg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tttgttcaag tgcttactgt tcattctcta aacttcaaga acgcgttatg tcgagtttcg 420

361 aaacaagttc acgaatgaca agtaagagat ttgaagttct tgcgcaatac agctcaaagc 420

.......... .......... .......... .......... .......... ORF 3

.......... .......... .......... .......... .......... ..........

421 tgccgaataa agagcaaacg cggacagtat taattttctg ttttcatttg aagaaaacag 480

421 acggcttatt tctcgtttgc gcctgtcata attaaaagac aaaagtaaac ttcttttgtc 480

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctgcggaatc gcaccgaatg cttgttgaag cctttggcga acaagtacca actgtgaaaa 540

481 gacgccttag cgtggcttac gaacaacttc ggaaaccgct tgttcatggt tgacactttt 540

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... SalI(585) ..........

541 cgtgtgaacg gtggtttcaa cgcttcaaaa gtggtgattt tgacgtcgac gacaaagagc 600

541 gcacacttgc caccaaagtt gcgaagtttt caccactaaa actgcagctg ctgtttctcg 600

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 acggaaaacc gccaaaaagg tacgaagacg ccgaactgca agcattattg gatgaagacg 660

601 tgccttttgg cggtttttcc atgcttctgc ggcttgacgt tcgtaataac ctacttctgc 660

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... NcoI(699) .......... ..........

661 atgctcaaac gtaagtttaa acatatatac taactaaccc atggattatt taaattttca 720

661 tacgagtttg cattcaaatt tgtatatatg attgattggg tacctaataa atttaaaagt 720

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ggcaaaaaca actcgcagag cagttggaag taagtcaaca agcagtttcc aatcgcttgc 780

721 ccgtttttgt tgagcgtctc gtcaaccttc attcagttgt tcgtcaaagg ttagcgaacg 780

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gagagatggg aaagattcag aaggtcggta gatgggtgcc acatgagttg aacgagaggc 840

781 ctctctaccc tttctaagtc ttccagccat ctacccacgg tgtactcaac ttgctctccg 840

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agatggagag gcgcaaaaac acatgcgaaa ttttgctttc acgatacaaa aggaagtcgt 900

841 tctacctctc cgcgtttttg tgtacgcttt aaaacgaaag tgctatgttt tccttcagca 900

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttttgcatcg tatcgttact ggagatgaaa aatggatctt ttttgttaat cctaaacgta 960

901 aaaacgtagc atagcaatga cctctacttt ttacctagaa aaaacaatta ggatttgcat 960

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aaaagtcata cgttgatcct ggacaaccgg ccacatcgac tgctcgaccg aatcgctttg 1020

961 ttttcagtat gcaactagga cctgttggcc ggtgtagctg acgagctggc ttagcgaaac 1020

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gcaagaagac gatgctctgt gtttggtggg atcagagcgg tgtcatttac tatgagctct 1080

1021 cgttcttctg ctacgagaca caaaccaccc tagtctcgcc acagtaaatg atactcgaga 1080

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... BclI(1126) ..........

1081 tgaaacccgg cgaaacggtg aatacggcac gctaccaaca acaattgatc aatttgaacc 1140

1081 actttgggcc gctttgccac ttatgccgtg cgatggttgt tgttaactag ttaaacttgg 1140

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtgcgcttca gagaaaacga ccggaatatc aaaaaagaca acacagggtc atttttctcc 1200

1141 cacgcgaagt ctcttttgct ggccttatag ttttttctgt tgtgtcccag taaaaagagg 1200

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atgacaacgc tccatcacat acggcaagag cggttcgcga cacgttggaa acactcaatt 1260

1201 tactgttgcg aggtagtgta tgccgttctc gccaagcgct gtgcaacctt tgtgagttaa 1260

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gggaagtgct tccgcatgcg gcttactcac cagacctggc cccatccgat taccacctat 1320

1261 cccttcacga aggcgtacgc cgaatgagtg gtctggaccg gggtaggcta atggtggata 1320

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcgcttcgat gggacacgca ctcgctgagc agcgcttcga ttcttacgaa agtgtgaaaa 1380

1321 agcgaagcta ccctgtgcgt gagcgactcg tcgcgaagct aagaatgctt tcacactttt 1380

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aatggctcga tgaatggttc gccgcaaaag acgatgagtt ctactggcgt ggaatccaca 1440

1381 ttaccgagct acttaccaag cggcgttttc tgctactcaa gatgaccgca ccttaggtgt 1440

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... NheI(1496)

1441 aattgcccga gagatgggaa aaatgtgtcg cgagcgacgg caaatacttt gaatagctag 1500

1441 ttaacgggct ctctaccctt tttacacagc gctcgctgcc gtttatgaaa cttatcgatc 1500

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 catgtatctg catatccttc aaatgttgtc catattgtat cagtaaatta taatgccccc 1560

1501 gtacatagac gtataggaag tttacaacag gtataacata gtcatttaat attacggggg 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tttttatata ttctccttat catgtaaatg ttctattgat tttttgctgg tgtaaaacgt 1620

1561 aaaaatatat aagaggaata gtacatttac aagataacta aaaaacgacc acattttgca 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttttatagta aatcctctcc acagtgaatt tgttaaattg tatcaaaaca agacattgat 1680

1621 aaaatatcat ttaggagagg tgtcacttaa acaatttaac atagttttgt tctgtaacta 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 taaatttgtt cgagacttgt ggattgtaaa atattttgca gaaaatggca atattcattt 1740

1681 atttaaacaa gctctgaaca cctaacattt tataaaacgt cttttaccgt tataagtaaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agaaacatta cttccataat aggaaaaact atttatgagt tttcatgtgc ctatgcaagt 1800

1741 tctttgtaat gaaggtatta tcctttttga taaatactca aaagtacacg gatacgttca 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tcccactctg tgtgaatttc atcatacaaa ttgagcaaac ataaggttta tgtccagtat 1860

1801 agggtgagac acacttaaag tagtatgttt aactcgtttg tattccaaat acaggtcata 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gggttctcag atgttttgta agagttgagc tgtcggtgaa tgccttctca cattgagcac 1920

1861 cccaagagtc tacaaaacat tctcaactcg acagccactt acggaagagt gtaactcgtg 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atggatacgg tctctctcca gtgtgtgttc tgaaataatg aaaaatagtc ttgcaatatg 1980

1921 tacctatgcc agagagaggt cacacacaag actttattac tttttatcag aacgttatac 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tttctactat tcgagaaaaa atgtaatttt ttttcttatg gtaacctgca atttatatat 2040

1981 aaagatgata agctcttttt tacattaaaa aaaagaatac cattggacgt taaatatata 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... EcoRV(2084) .........

2041 tttttatttg ccgttattga cattattata agaaattgga ggatatcaca aatatcgtaa 2100

2041 aaaaataaac ggcaataact gtaataatat tctttaacct cctatagtgt ttatagcatt 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 attctccaaa attttgacac ttcactttaa agacaaatgc ggtttttccg agtgtgtctc 2160

2101 taagaggttt taaaactgtg aagtgaaatt tctgtttacg ccaaaaaggc tcacacagag 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gccacgatct cagtcataat ggtcgctaca ggtgccaaac tttaggcatt tgttatattt 2220

2161 cggtgctaga gtcagtatta ccagcgatgt ccacggtttg aaatccgtaa acaatataaa 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gatgcagaaa accaagcaca tgaaaattaa aattgtcaag tagacaatga aatccagaca 2280

2221 ctacgtcttt tggttcgtgt acttttaatt ttaacagttc atctgttact ttaggtctgt 2280

.......... .......... .......... .......... .......... ..........

.......... ...... EcoRI(2303) SmaI(2317),BamHI(2321) SpeI(2327)

2281 ttgttatgat tcattttttt gcgaattcct gcagcccggg ggatccacta gtcggccgta 2340

2281 aacaatacta agtaaaaaaa cgcttaagga cgtcgggccc cctaggtgat cagccggcat 2340

.......... .......... .......... .......... .......... ..........

ApaI(2346) .......... .......... .......... .......... ..........

2341 cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 2400

2341 gcccgggaaa gcagagcgcg caaagccact actgccactt ttggagactg tgtacgtcga 2400

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

2401 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 2460

2401 gggcctctgc cagtgtcgaa cagacattcg cctacggccc tcgtctgttc gggcagtccc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 2520

2461 gcgcagtcgc ccacaaccgc ccacagcccc gaccgaattg atacgccgta gtctcgtcta 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 2580

2521 acatgactct cacgtggtat acgccacact ttatggcgtg tctacgcatt cctcttttat 2580

.......... .......... .......... .......... .......... ..........

......... AflII(2595) ......... .......... .......... ..........

2581 ccgcatcagg cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 2640

2581 ggcgtagtcc gccggaattc ccggagcact atgcggataa aaatatccaa ttacagtact 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 2700

2641 attattacca aagaatctgc agtccaccgt gaaaagcccc tttacacgcg ccttggggat 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 2760

2701 aaacaaataa aaagatttat gtaagtttat acataggcga gtactctgtt attgggacta 2760

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 2820

2761 tttacgaagt tattataact ttttccttct catactcata agttgtaaag gcacagcggg 2820

.......... .......... .......... ....... ORF 3 Ampicillin .......

.......... .......... .......... .......... .......... ..........

2821 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 2880

2821 aataagggaa aaaacgccgt aaaacggaag gacaaaaacg agtgggtctt tgcgaccact 2880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 2940

2881 ttcattttct acgacttcta gtcaacccac gtgctcaccc aatgtagctt gacctagagt 2940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 3000

2941 tgtcgccatt ctaggaactc tcaaaagcgg ggcttcttgc aaaaggttac tactcgtgaa 3000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 3060

3001 aatttcaaga cgatacaccg cgccataata gggcataact gcggcccgtt ctcgttgagc 3060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 3120

3061 cagcggcgta tgtgataaga gtcttactga accaactcat gagtggtcag tgtcttttcg 3120

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 3180

3121 tagaatgcct accgtactgt cattctctta atacgtcacg acggtattgg tactcactat 3180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 3240

3181 tgtgacgccg gttgaatgaa gactgttgct agcctcctgg cttcctcgat tggcgaaaaa 3240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 3300

3241 acgtgttgta ccccctagta cattgagcgg aactagcaac ccttggcctc gacttacttc 3300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... FspI(3357)

3301 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 3360

3301 ggtatggttt gctgctcgca ctgtggtgct acggacatcg ttaccgttgt tgcaacgcgt 3360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 3420

3361 ttgataattg accgcttgat gaatgagatc gaagggccgt tgttaattat ctgacctacc 3420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 3480

3421 tccgcctatt tcaacgtcct ggtgaagacg cgagccggga aggccgaccg accaaataac 3480

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 3540

3481 gactatttag acctcggcca ctcgcaccca gagcgccata gtaacgtcgt gaccccggtc 3540

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 3600

3541 taccattcgg gagggcatag catcaataga tgtgctgccc ctcagtccgt tgatacctac 3600

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 3660

3601 ttgctttatc tgtctagcga ctctatccac ggagtgacta attcgtaacc attgacagtc 3660

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 3720

3661 tggttcaaat gagtatatat gaaatctaac taaattttga agtaaaaatt aaattttcct 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 3780

3721 agatccactt ctaggaaaaa ctattagagt actggtttta gggaattgca ctcaaaagca 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 3840

3781 aggtgactcg cagtctgggg catcttttct agtttcctag aagaactcta ggaaaaaaag 3840

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3841 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 3900

3841 acgcgcatta gacgacgaac gtttgttttt ttggtggcga tggtcgccac caaacaaacg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 3960

3901 gcctagttct cgatggttga gaaaaaggct tccattgacc gaagtcgtct cgcgtctatg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 4020

3961 gtttatgaca ggaagatcac atcggcatca atccggtggt gaagttcttg agacatcgtg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 4080

4021 gcggatgtat ggagcgagac gattaggaca atggtcaccg acgacggtca ccgctattca 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 4140

4081 gcacagaatg gcccaacctg agttctgcta tcaatggcct attccgcgtc gccagcccga 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 4200

4141 cttgcccccc aagcacgtgt gtcgggtcga acctcgcttg ctggatgtgg cttgactcta 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 4260

4201 tggatgtcgc actcgtaact ctttcgcggt gcgaagggct tccctctttc cgcctgtcca 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 4320

4261 taggccattc gccgtcccag ccttgtcctc tcgcgtgctc cctcgaaggt ccccctttgc 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 4380

4321 ggaccataga aatatcagga cagcccaaag cggtggagac tgaactcgca gctaaaaaca 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 4440

4381 ctacgagcag tccccccgcc tcggatacct ttttgcggtc gttgcgccgg aaaaatgcca 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 4500

4441 aggaccggaa aacgaccgga aaacgagtgt acaagaaagg acgcaatagg ggactaagac 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 4560

4501 acctattggc ataatggcgg aaactcactc gactatggcg agcggcgtcg gcttgctggc 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 4620

4561 tcgcgtcgct cagtcactcg ctccttcgcc ttctcgcggg ttatgcgttt ggcggagagg 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 4680

4621 ggcgcgcaac cggctaagta attacgtcga ccgtgctgtc caaagggctg acctttcgcc 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 4740

4681 cgtcactcgc gttgcgttaa ttacactcaa tcgagtgagt aatccgtggg gtccgaaatg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 4800

4741 tgaaatacga aggccgagca tacaacacac cttaacactc gcctattgtt aaagtgtgtc 4800

.......... lac_pro .......... ....... M13_pUC_rev_pri .......

.......... .......... .......... .......... .......... ..........

4801 gaaacagct. .......... .......... .......... .......... .......... 4809

4801 ctttgtcga. .......... .......... .......... .......... .......... 4809

.......... .......... .......... .......... .......... ..........