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    Plasmid map of pJRtac99

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    Vendor: ATCC
    Backbone Size (bp): 1392
    Catalog Number: 87018
    Type of Vector: RNAi
    GenBank Accession Number: X69551
    Comments: MCS. oligonucleotide linker. in GenBank. Restriction digests of the clone give the following sizes (kb): EcoRI--1.3; HindIII--1.3; BglII--1.3. (ATCC staff) Expression vector permitting production of heterologous peptides fused to active chloramphenicol acetyltransferase. The replicon is derived from ColE1 and does not contain the RNAII promoter nor most of the antisense RNAI control element coding sequence. This allows compatibility with other ColE1 plasmids. Replication depends on read-through from the tac promoter. Replication may be negatively affected by cloning of large inserts or sequences containing a transcriptional terminator. Repression of this high copy number plasmid is hard to achieve, even using hosts overproducing lacIq. Escherichia coli WK6 is recommended for inducible expression. The vector contains the following restriction sites (approximate kb from nt 1): BglII--0.79; ClaI--0.11; EcoRI--0.33; HindIII--0.02; NcoI--0.12; PstI--0.75; PvuII--0.23. The order o
    f the major features of the plasmid is: HindIII - tac promoter - NcoI - cat/ScaI/MCS/BglII - HyRep2 ori. [1] Growth: LB plus chloramphenicol (25 ug/ml) 37C Hosts: E.coli, E.coli BMH71-18.
    Related vectors: pGV451 from pBR327.

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