Sequence of pJRtac99

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Sequence of pJRtac99

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Sequence of pJRtac99 : 1392 BP; 361 A; 325 C; 359 G; 347 T; 0 other;

......... HindIII(20) ......... .......... AseI(49) ..........

1 acgccagcaa cgcggcccga agcttactcc ccatccccct gttgacaatt aatcatcggc 60

1 tgcggtcgtt gcgccgggct tcgaatgagg ggtaggggga caactgttaa ttagtagccg 60

.......... .......... .......... .......... ......... tac_promoter

.......... .......... .......... .......... ..... ClaI(114),NcoI(119)

61 tcgtataatg tgtggaattg tgagcggata acaatttcac acaggaaaca ggatcgatcc 120

61 agcatattac acaccttaac actcgcctat tgttaaagtg tgtcctttgt cctagctagg 120

tac_promoter ........ ...... M13_pUC_rev_primer ...... ..........

.......... .......... .......... .......... .......... ..........

121 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 180

121 tacctctttt tttagtgacc tatatggtgg caactatata gggttaccgt agcatttctt 180

.......... Orf frame 1 CAT/CamR(w/ gaps) .......... ..........

.......... .......... .......... .......... .......... PvuII(234)

181 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 240

181 gtaaaactcc gtaaagtcag tcaacgagtt acatggatat tggtctggca agtcgaccta 240

.......... Orf frame 1 CAT/CamR(w/ gaps) .......... ..........

.......... .......... .......... .......... .......... ..........

241 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 300

241 taatgccgga aaaatttctg gcatttcttt ttattcgtgt tcaaaatagg ccggaaataa 300

.......... Orf frame 1 CAT/CamR(w/ gaps) .......... ..........

.......... .......... .......... EcoRI(334) .......... ..........

301 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt 360

301 gtgtaagaac gggcggacta cttacgagta ggccttaagg cataccgtta ctttctgcca 360

.......... Orf frame 1 CAT/CamR(w/ gaps) .......... ..........

.......... .......... .......... .......... .......... ..........

361 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 420

361 ctcgaccact ataccctatc acaagtggga acaatgtggc aaaaggtact cgtttgactt 420

.......... Orf frame 1 CAT/CamR(w/ gaps) .......... ..........

.......... .......... .......... .......... .......... ..........

421 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 480

421 tgcaaaagta gcgagacctc acttatggtg ctgctaaagg ccgtcaaaga tgtgtatata 480

.......... Orf frame 1 CAT/CamR(w/ gaps) .......... ..........

.......... .......... .......... .......... .......... ..........

481 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 540

481 agcgttctac accgcacaat gccacttttg gaccggataa agggatttcc caaataactc 540

.......... Orf frame 1 CAT/CamR(w/ gaps) .......... ..........

.......... .......... .......... .......... .......... ..........

541 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 600

541 ttatacaaaa agcagagtcg gttagggacc cactcaaagt ggtcaaaact aaatttgcac 600

.......... Orf frame 1 CAT/CamR(w/ gaps) .......... ..........

MscI(601) .......... .......... .......... .......... ..........

601 gccaatatgg acaacttctt cgcccccgtt ttcacaatgg gcaaatatta tacgcaaggc 660

601 cggttatacc tgttgaagaa gcgggggcaa aagtgttacc cgtttataat atgcgttccg 660

.......... Orf frame 1 CAT/CamR(w/ gaps) .......... ..........

.......... .......... .......... .......... .......... ..........

661 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat 720

661 ctgttccacg actacggcga ccgctaagtc caagtagtac ggcaaacact accgaaggta 720

.......... Orf frame 1 CAT/CamR(w/ gaps) .......... ..........

.......... .......... ..... Pstl(757),SalI(760) ..... SmaI(774)

721 gtcggcagaa tgcttaatga attacaacag tactgcagcg tcgaccaggg acccgggtaa 780

721 cagccgtctt acgaattact taatgttgtc atgacgtcgc agctggtccc tgggcccatt 780

.......... Orf frame 1 CAT/CamR(w/ gaps) .......... ..........

.......... BglII(793) .......... .......... .......... ..........

781 tgaagagtaa ctagatctac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca 840

781 acttctcatt gatctagatg acgctactca ccgtcccgcc ccgcattaaa aaaattccgt 840

.......... ...... CAT/CamR(w/ gaps) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gttattggtg cccttaaacg cctggtgcta cgcctgaata agtgataata agcggatgaa 900

841 caataaccac gggaatttgc ggaccacgat gcggacttat tcactattat tcgcctactt 900

.......... .......... .......... .......... .......... ..........

.......... BstBI(911) .......... .......... .......... ..........

901 tggcagaaat tcgaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 960

901 accgtcttta agcttgaccg aagtcgtctc gcgtctatgg tttatgacag gaagatcaca 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1020

961 tcggcatcaa tccggtggtg aagttcttga gacatcgtgg cggatgtatg gagcgagacg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1080

1021 attaggacaa tggtcaccga cgacggtcac cgctattcag cacagaatgg cccaacctga 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... ApaLI(1133)

1081 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1140

1081 gttctgctat caatggccta ttccgcgtcg ccagcccgac ttgcccccca agcacgtgtg 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag 1200

1141 tcgggtcgaa cctcgcttgc tggatgtggc ttgactctat ggatgtcgca ctcgtaactc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1260

1201 tttcgcggtg cgaagggctt ccctctttcc gcctgtccat aggccattcg ccgtcccagc 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1320

1261 cttgtcctct cgcgtgctcc ctcgaaggtc cccctttgcg gaccatagaa atatcaggac 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1380

1321 agcccaaagc ggtggagact gaactcgcag ctaaaaacac tacgagcagt ccccccgcct 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcctatggaa aa........ .......... .......... .......... .......... 1392

1381 cggatacctt tt........ .......... .......... .......... .......... 1392

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-1392 Reference comment: pJRtac99 from Tn9 & pDR540 & pGV451, HyRep2 ori Reference author: Robben J., Massie G., Bosmans E., Wellens B., Volckaert G.; Reference title: "An Escherichia coli plasmid vector system for high-level production Reference title: and purification of heterologous peptides fused to active Reference title: chloramphenicol acetyltransferase"; Reference location: Gene 126:109-113(1993).
Reference number: [2] Reference comment: pJRtac99 from Tn9 & pDR540 & pGV451, HyRep2 ori Reference author: Dekeyzer N., Volckaert G.N.; Reference title: "Cloning, expression and purification of a sarcoplasmic Reference title: calcium-binding protein from the sandworm Nereis diversicolor via Reference title: a fusion product with chloramphenicol acetyltransferase"; Reference location: Protein Engineering 7:125-130(1994).