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View the sequence with features on line
Backbone size: 4746
Catalog Number: 37127
GenBank Accession Number:X51449
Comments:Created by Moore, July 1995, under contract with NCBI. Similar to pDS20. A terminator cloning plasmid vector. (ATCC staff) Insertion of terminator sequences at the cloning sites causes the galK+ phenotype to become galK-. galK+ plasmids are lethal on galETK hosts because of galactose-1-phosphate accumulation, so terminator-containing clones survive in galactose media.  Medium is 1227 LB plus ampicillin. Hosts: E.coli N100, E.coli, E.coli S165. Related vectors: pK01, gal operon promoter. (Information source: VectorDB.)