NONONLOCUS pKH80 12637 bp DNA SYN 03-Oct-2007 DEFINITION pKH80 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..12637 /organism="pKH80" /mol_type="other DNA" misc_feature complement(438..457) /label="pBRrevBam_primer" CDS 86..1276 /label="ORF frame 2" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST*" gene complement(86..1276) /label="tet" /gene="tet" misc_feature 1915..2106 /label="ROP" misc_feature complement(2100..2122) /label="pGEX_3_primer" rep_origin complement(2519..3138) /label="pBR322_origin" CDS complement(3293..4153) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(3293..4153) /label="Ampicillin" /gene="Ampicillin" promoter complement(4195..4223) /label="AmpR_promoter" CDS 4850..5704 /label="ORF frame 2" /translation="MLYATVHLDERVPHMHFGFVPLTEDGRLSAKEQLGNKKDFTQLQ DRFNEYVNEKGYELERGTSKEVTEREHKAMDQYKKDTVFHKQELQEVKDELQKANKQL QSGIEHMRSTKPFDYENERTGLFSGREETGRKILTADEFERLQETISSAERIVDDYEN IKSTDYYTENQELKKRRESLKEVVNTWKEGYHEKSKEVNKLKRENDSLNEQLNVSEKF QASTVTLYRAARANFPGFEKGFNRLKEKFFNDSKFERVGQFMDVVQDNVQKVDRKREK QRTDDLEM*" CDS complement(7075..7809) /label="eryth" /gene="eryth" /note="ORF frame 1" /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF TLELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNI PYNISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPR EYFHPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH AGIDDLNNISFEQFLSLFNSYKLFNK*" gene complement(7075..7809) /label="eryth" /gene="eryth" CDS complement(10288..11058) /label="ORF frame 1" /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLG RQTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLT HGQFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQG PTTFLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSG QLSDSEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF*" CDS complement(11218..12222) /label="ORF frame 1" /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVD YFELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKH GIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK NLVGFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLD YDPNVKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHR KRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE *" ORIGIN 1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg 541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct 601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat 661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa 901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt 961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc 1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca 1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc 1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat 1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg 1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga 1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac 1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg 1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg 1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc 1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg 1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca 1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga 1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa 1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt 1861 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc 1921 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt 1981 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac 2041 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac 2101 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 2161 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc 2221 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg 2281 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 2341 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 2401 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 2461 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 2521 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 2581 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 2641 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 2701 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 2761 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 2821 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 2881 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 2941 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 3001 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 3061 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 3121 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 3181 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 3241 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 3301 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 3361 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 3421 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 3481 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 3541 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 3601 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 3661 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 3721 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 3781 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 3841 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 3901 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 3961 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 4021 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 4081 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 4141 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 4201 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 4261 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 4321 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attccttagt gctttcatag 4381 attaaactca catcacgctt taaatcgctt attttagact ttaaagactt gttttcttca 4441 agcaactcat tataatcatt tacattttca ttaaatcgct ctacaagacc actatatttt 4501 tctttaactt gcccatgttc tttacttaat tttttatatt ctctcgccat atcagtactc 4561 atgagatttc taacatgctg ttttaaccta tcgttatctc tcgcagcagt cactaagttt 4621 ttataatcac gctccgatat aacaacattt ttggttggtt tcttttctgt tttcattatt 4681 tcttttccca aaccaaacat ggacttttca cccgttggca cttcaacact tttcatgtgt 4741 cgtttcgctg gtacttctaa atctgattta actttatcgc tataagcagt ccattcatct 4801 tttttaactg ctaaattttt ttctagaaaa tgaatacggt aaggaaaata tgctgtatgc 4861 gactgtccat ctggatgaaa gagtcccaca tatgcacttt ggttttgtcc ctttaacaga 4921 ggacgggaga ttgtctgcaa aagaacagtt aggcaacaag aaagacttta ctcaattaca 4981 agatagattt aatgagtatg tgaatgagaa aggttatgaa cttgaaagag gcacgtccaa 5041 agaggttaca gaacgagaac ataaagcgat ggatcagtac aagaaagata ctgtatttca 5101 taaacaggaa ctgcaagaag ttaaggatga gttacagaag gcaaataagc agttacagag 5161 tggaatagag catatgaggt ctacgaaacc ctttgattat gaaaatgagc gtacaggttt 5221 gttctctgga cgtgaagaga ctggtagaaa gatattaact gctgatgaat ttgaacgcct 5281 gcaagaaaca atctcttctg cagaacggat tgttgatgat tacgaaaata ttaagagcac 5341 agactattac acagaaaatc aagaattaaa aaaacgtaga gagagtttga aagaagtagt 5401 gaatacatgg aaagaggggt atcacgaaaa aagtaaagag gttaataaat taaagcgaga 5461 gaatgatagt ttgaatgagc agttgaatgt atcagagaaa tttcaagcta gtacagtgac 5521 tttatatcgt gctgcgaggg cgaatttccc tgggtttgag aaagggttta ataggcttaa 5581 agagaaattc tttaatgatt ccaaatttga gcgtgtggga cagtttatgg atgttgtaca 5641 ggataatgtc cagaaggtcg atagaaagcg tgagaaacag cgtacagacg atttagagat 5701 gtagaggtac ttttatgccg agaaaacttt ttgcgtgtga cagtccttaa aatatactta 5761 gagcgtaagc gaaagtagta gcgacagcta ttaactttcg gttgcaaagc tctaggattt 5821 ttaatggacg cagcgcatca cacgcaaaaa ggaaattgga ataaatgcga aatttgagat 5881 gttaattaaa gacctttttg aggtcttttt ttcttagatt tttggggtta tttaggggag 5941 aaaacatagg ggggtactac gacctccccc ctaggtgtcc attgtccatt gtccaaacaa 6001 ataaataaat attgggtttt taatgttaaa aggttgtttt ttatgttaaa gtgaaaaaaa 6061 cagatgttgg gaggtacagt gatggttgta gatagaaaag aagagaaaaa agttgctgtt 6121 actttaagac ttacaacaga agaaaatgag atattaaata gaatcaaaga aaaatataat 6181 attagcaaat cagatgcaac cggtattcta ataaaaaaat atgcaaagga ggaatacggt 6241 gcattttaaa caaaaaaaga tagacagcac tggcatgctg cctatctatg actaaatttt 6301 gttaagtgta ttagcaccgt tattatatca tgagcgaaaa tgtaataaaa gaaactgaaa 6361 acaagaaaaa ttcaagagga cgtaattgga catttgtttt atatccagaa tcagcaaaag 6421 ccgagtggtt agagtattta aaagagttac acattcaatt tgtagtgtct ccattacatg 6481 atagggatac tgatacagaa ggtaggatga aaaaagagca ttatcatatt ctagtgatgt 6541 atgagggtaa taaatcttat gaacagataa aaataattaa cagaagaatt gaatgcgact 6601 attccgcaga ttgcaggaag tgtgaaaggt cttgtgagat atatgcttca catggacgat 6661 cctaataaat ttaaatatca aaaagaagat atgatagttt atggcggtgt agatgttgat 6721 gaattattaa agaaaacaac aacagataga tataaattaa ttaaagaaat gattgagttt 6781 attgatgaac aaggaatcgt agaatttaag agtttaatgg attatgcaat gaagtttaaa 6841 tttgatgatt ggttcccgct tttatgtgat aactcggcgt atgttattca agaatatata 6901 aaatcaaatc ggtataaatc tgaccgatag attttgaatt taggtgtcac aagacactct 6961 tttttcgcac cagcgaaaac tggtttaagc cgactgcgca aaagacataa tcgattcaca 7021 aaaaataggc acacgaaaaa caagttaagg gatgcagttt atgcatccct taacttactt 7081 attaaataat ttatagctat tgaaaagaga taagaattgt tcaaagctaa tattgtttaa 7141 atcgtcaatt cctgcatgtt ttaaggaatt gttaaattga ttttttgtaa atattttctt 7201 gtattctttg ttaacccatt tcataacgaa ataattatac ttttgtttat ctttgtgtga 7261 tattcttgat ttttttctac ttaatctgat aagtgagcta ttcactttag gtttaggatg 7321 aaaatattct cttggaacca tacttaatat agaaatatca acttctgcca ttaaaagtaa 7381 tgccaatgag cgttttgtat ttaataatct tttagcaaac ccgtattcca cgattaaata 7441 aatctcatta gctatactat caaaaacaat tttgcgtatt atatccgtac ttatgttata 7501 aggtatatta ccatatattt tataggattg gtttttagga aatttaaact gcaatatatc 7561 cttgtttaaa acttggaaat tatcgtgatc aacaagttta ttttctgtag ttttgcataa 7621 tttatggtct atttcaatgg cagttacgaa attacacctc tttactaatt caagggtaaa 7681 atggcctttt cctgagccga tttcaaagat attatcatgt tcatttaatc ttatatttgt 7741 cattatttta tctatattat gttttgaagt aataaagttt tgactgtgtt ttatattttt 7801 ctcgttcatt ataaccctct ttaatttggt tatatgaatt ttgcttatta acgattcatt 7861 ataaccactt attttttgtt tggttgataa tgaactgtgc tgattacaaa aatactaaaa 7921 atgcccatat tttttcctcc ttataaaatt agtataatta tagcacgagc tctgataaat 7981 atgaacatga tgagtgatcg ttaaatttat actgcaatcg gatgcgatta ttgaataaaa 8041 gatatgagag atttatctaa tttctttttt cttgtaaaaa aagaaagttc ttaaaggttt 8101 tatagttttg gtcgtagagc acacggttta acgacttaat tacgaagtaa ataagtctag 8161 tgtgttagac tttatgaaat ctatatacgt ttatatatat ttattatccg gaggtgtagc 8221 atgtctcatt caattttgag ggttgccaga gttaaaggat caagtaatac aaacgggata 8281 caaagacata atcaaagaga gaataaaaac tataataata aagacataaa tcatgaggaa 8341 acatataaaa attatgattt gattaacgca caaaatataa agtataaaga taaaattgat 8401 gaaacgattg atgagaatta ttcagggaaa cgtaaaattc ggtcagatgc aattcgacat 8461 gtggacggac tggttacaag tgataaagat ttctttgatg atttaagcgg agaagaaata 8521 gaacgatttt ttaaagatag cttggagttt ctagaaaatc aatctctttt tccaaagttt 8581 gttttttaaa tttagctgtc tcaatatgtt tacggtcaga gccacgttca ccacgcttca 8641 actcaaaacc ctgttttttc atatgctcgg ggaatttatc ttgtagccat aacagttctt 8701 gacgattaaa cacatttttt ccttgcagtt ttccatcacg cataggcaca acacctaaat 8761 gcatgtgagg ggtttgctca tcattatgaa ctgttgcata agcaatattt tgcttgccat 8821 atcgttcgga aaataattta taactttcct caaaaaatcg tttttgttct cctggatcca 8881 gttgctcaaa aaaatctcgg tcagatgtta ctagcaactc atttacaaga acagcatctt 8941 tcctccgttt ttcttgtacc tgttttttgt gattcaataa tttctttgac acgttcgttg 9001 taatcaatat ttttatcatt tttcaaatca taattttcac gtgttcgctc atggtcaata 9061 tcatcattcg ttctactttt tcgctctctt tgattatgaa attgcatgcc ttttagtcca 9121 gctgatttca ctttttgcat tctacaaact gcataactca tatgtaaatc gctccttttt 9181 aggtggcaca aatgtgaggc attttcgctc tttccggcaa ccacttccaa gtaaagtata 9241 acacactata ctttatattc ataaagtgtg tgctctgcga ggctgtcggc agtgccgacc 9301 aaaaccataa aacctttaag acctttcttt tttttacgag aaaaaagaaa caaaaaaacc 9361 tgccctctgc cacctcagca aaggggggtt ttgctctcgt gctcgtttaa aaatcagcaa 9421 gggacaggta gtattttttg agaagatcac tcaaaaaatc tccaccttta aacccttgcc 9481 aatttttatt ttgtccgttt tgtctagctt accgaaagcc agactcagca agaataaaat 9541 ttttattgtc tttcggtttt ctagtgtaac ggacaaaacc actcaaaata aaaaagatac 9601 aagagaggtc tctcgtatct tttattcagc aatcgcgccc gattgctgaa cagattaata 9661 atagatttta gctttttatt tgttgaaaaa agctaatcaa attgttgtcg ggatcaatta 9721 ctgcaaagtc tcgttcatcc caccactgat cttttaatga tgtattgggg tgcaaaatgc 9781 ccaaaggctt aatatgttga tataattcat caattccctc tacttcaatg cggcaactag 9841 cagtaccagc aataaacgac tccgcacctg tacaaaccgg tgaatcatta ctacgagagc 9901 gccagcttca tcacttgcct cccatagatg aatccgaacc tcattacaca ttagaactgc 9961 gaatccatct tcatggtgaa ccaaagtgaa acctagttta tcgcaataaa aacctatact 10021 ctttttaata tccccgactg gcaatgccgg gatagactgt aacattctca cgcataaaat 10081 cccctttcat tttctaatgt aaatctatta ccttattatt aattcaattc gctcataatt 10141 aatccttttt cttattacgc aaaatggccc gatttaagca caccctttat tccgttaatg 10201 cgccatgaca gccatgataa ttactaatac taggagaagt taataaatac gtaaccaaca 10261 tgattaacaa ttattagagg tcatcgttca aaatggtatg cgttttgaca catccactat 10321 atatccgtgt cgttctgtcc actcctgaat cccattccag aaattctcta gcgattccag 10381 aagtttctca gagtcggaaa gttgaccaga cattacgaac tggcacagat ggtcataacc 10441 tgaaggaaga tctgattgct taactgcttc agttaagacc gaagcgctcg tcgtataaca 10501 gatgcgatga tgcagaccaa tcaacatggc acctgccatt gctacctgta cagtcaagga 10561 tggtagaaat gttgtcggtc cttgcacacg aatattacgc catttgcctg catattcaaa 10621 cagctcttct acgataaggg cacaaatcgc atcgtggaac gtttgggctt ctaccgattt 10681 agcagtttga tacactttct ctaagtatcc acctgaatca taaatcggca aaatagagaa 10741 aaattgacca tgtgtaagcg gccaatctga ttccacctga gatgcataat ctagtagaat 10801 ctcttcgcta tcaaaattca cttccacctt ccactcaccg gttgtccatt catggctgaa 10861 ctctgcttcc tctgttgaca tgacacacat catctcaata tccgaatagg gcccatcagt 10921 ctgacgacca agagagccat aaacaccaat agccttaaca tcatccccat atttatccaa 10981 tattcgttcc ttaatttcat gaacaatctt cattctttct tctctagtca ttattattgg 11041 tccattcact attctcattc ccttttcaga taattttaga tttgcttttc taaataagaa 11101 tatttggaga gcaccgttct tattcagcta ttaataactc gtcttcctaa gcatccttca 11161 atccttttaa taacaattat agcatctaat cttcaacaaa ctggcccgtt tgttgaacta 11221 ctctttaata aaataatttt tccgttccca attccacatt gcaataatag aaaatccatc 11281 ttcatcggct ttttcgtcat catctgtatg aatcaaatcg ccttcttctg tgtcatcaag 11341 gtttaatttt ttatgtattt cttttaacaa accaccatag gagattaacc ttttacggtg 11401 taaaccttcc tccaaatcag acaaacgttt caaattcttt tcttcatcat cggtcataaa 11461 atccgtatcc tttacaggat attttgcagt ttcgtcaatt gccgattgta tatccgattt 11521 atatttattt ttcggtcgaa tcatttgaac ttttacattt ggatcatagt ctaatttcat 11581 tgcctttttc caaaattgaa tccattgttt ttgattcacg tagttttctg tattcttaaa 11641 ataagttggt tccacacata ccaatacatg catgtgctga ttataagaat tatctttatt 11701 atttattgtc acttccgttg cacgcataaa accaacaaga tttttattaa tttttttata 11761 ttgcatcatt cggcgaaatc cttgagccat atctgacaaa ctcttattta attcttcgcc 11821 atcataaaca tttttaactg ttaatgtgag aaacaaccaa cgaactgttg gcttttgttt 11881 aataacttca gcaacaacct tttgtgactg aatgccatgt ttcattgctc tcctccagtt 11941 gcacattgga caaagcctgg atttacaaaa ccacactcga tacaactttc tttcgcctgt 12001 ttcacgattt tgtttatact ctaatatttc agcacaatct tttactcttt cagccttttt 12061 aaattcaaga atatgcagaa gttcaaagta atcaacatta gcgattttct tttctctcca 12121 tggtctcact tttccacttt ttgtcttgtc cactaaaacc cttgattttt catctgaata 12181 aatgctacta ttaggacaca taatattaaa agaaaccccc atctatttag ttatttgttt 12241 agtcacttat aactttaaca gatggggttt ttctgtgcaa ccaattttaa gggttttcaa 12301 tactttaaaa cacatacata ccaacacttc aacgcacctt tcagcaacta aaataaaaat 12361 gacgttattt ctatatgtat caagataaga aagaacaagt tcaaaaccat caaaaaaaga 12421 caccttttca ggtgcttttt ttattttata aactcattcc ctgatctcga cttcgttctt 12481 tttttacctc tcggttatga gttagttcaa attcgttctt tttaggttct aaatcgtgtt 12541 tttcttggaa ttgtgctgtt ttatccttta ccttgtctac aaacccctta aaaacgtttt 12601 taaaggcttt taagccgtct gtacgttcct taaggaa // ]1pKH80 "Times New RomanNOC]1 pBRrevBam_pri"Times New RomanNOC]1VORF 2"Times New RomanNOCɆ]1Vtet"Times New Roman*NO]1{:ROP"Times New RomanNOC]14J pGEX_3_pri"Times New RomanNOC]1 B  pBR322_origin"Times New RomanNOCɆ]1 9ORF 2"Times New RomanNO]1 9 Ampicillin"Times New RomanNOC]1cAmpR_pro"Times New RomanNOC]1HORF 2"Times New RomanNOCɆ]1ORF 1"Times New RomanNO]1eryth"Times New RomanNOC]10(2+ORF 1"Times New RomanNOC]1+/ORF 1"Times New Roman NOHindIII"Times New Roman]1hhyhoH`o NOEcoRV(187),NheI(229)"Times New Roman]1|u[=[ NOSalI"Times New Roman]1hYh NOEagI(939),NruI(974)"Times New Roman]1te, NOMscI"Times New Roman]1  M NOAatII"Times New Roman ]1+$ NOHpaI"Times New Roman, ]19BVV'e NOBclI"Times New Roman]1#)0)7 NOSacI"Times New Roman#]1 s! NOPmlI"Times New RomanP#]1~t7t NOBglII"Times New Roman(]1C NOApaI"Times New Roman*]1a NONcoI"Times New RomanV/]1,'y"gXEg NOAflII"Times New RomanU1]1cBwJU(FlU NOtest"Times New Romanl]1dS