Fusion proteins or tags: GFP
Terminal: C terminal on backbone
Vector backbone: pLL3.7
Backbone manufacturer: N/A
Type of vector: Mammalian expression,Lentiviral,RNAi,Cre/Lox
Backbone size (bp): 8500
Cloning site 5': HpaI
Site destroyed during cloning: No
Cloning site 3': XhoI
Site destroyed during cloning: No
5' Sequencing primer: mU6-F
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Stephan Kissler
Comments: pLB is a modification of pLL3.7. Two genetic elements known to prevent epigenetic silencing were added. A fragment of one antirepressor element (#40) was cloned upstream of the U6 promoter and a scaffold-attached region (SAR) was cloned downstream of GFP. Note: A single base pair deletion at position 11 of the U6 promoter in this plasmid does not impair the efficacy of this reagent.
Article: In vivo RNA interference demonstrates a role for Nramp1 in modifying susceptibility to type 1 diabetes. Kissler S et al. (Nat Genet. 2006 Apr . 38(4):479-83. Pubmed)