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    Plasmid map of pLB

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    Fusion proteins or tags: GFP
    Terminal: C terminal on backbone
    Vector backbone: pLL3.7
    Backbone manufacturer: N/A
    Type of vector: Mammalian expression,Lentiviral,RNAi,Cre/Lox
    Backbone size (bp): 8500
    Cloning site 5': HpaI
    Site destroyed during cloning: No
    Cloning site 3': XhoI
    Site destroyed during cloning: No
    5' Sequencing primer: mU6-F
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Plasmid Provided In: DH5a
    Principal Investigator: Stephan Kissler
    Comments: pLB is a modification of pLL3.7. Two genetic elements known to prevent epigenetic silencing were added. A fragment of one antirepressor element (#40) was cloned upstream of the U6 promoter and a scaffold-attached region (SAR) was cloned downstream of GFP. Note: A single base pair deletion at position 11 of the U6 promoter in this plasmid does not impair the efficacy of this reagent.
    Article: In vivo RNA interference demonstrates a role for Nramp1 in modifying susceptibility to type 1 diabetes. Kissler S et al. (Nat Genet. 2006 Apr . 38(4):479-83. Pubmed)

    image of pLB

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