Sequence of pLB

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

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Sequence of pLB

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Sequence of pLB : 8831 BP; 2337 A; 2057 C; 2112 G; 2325 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 60

1 cagctgccta gccctctaga gggctagggg ataccacgtg agagtcatgt tagacgagac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 120

61 tacggcgtat caattcggtc atagacgagg gacgaacaca caacctccag cgactcatca 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 180

121 cgcgctcgtt ttaaattcga tgttgttccg ttccgaactg gctgttaacg tacttcttag 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 240

181 acgaatccca atccgcaaaa cgcgacgaag cgctacatgc ccggtctata tgcgcaactg 240

.......... .......... .......... .......... .......... ..........

.......... SpeI(252) .......... .......... .......... ..........

241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 300

241 taactaataa ctgatcaata attatcatta gttaatgccc cagtaatcaa gtatcgggta 300

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 360

301 tatacctcaa ggcgcaatgt attgaatgcc atttaccggg cggaccgact ggcgggttgc 360

.......... ......... CMV_imm_pro CAG_enh ......... ..........

.......... .......... .......... .......... .......... ..........

361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 420

361 tgggggcggg taactgcagt tattactgca tacaagggta tcattgcggt tatccctgaa 420

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 480

421 aggtaactgc agttacccac ctcataaatg ccatttgacg ggtgaaccgt catgtagttc 480

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 540

481 acatagtata cggttcatgc gggggataac tgcagttact gccatttacc gggcggaccg 540

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 600

541 taatacgggt catgtactgg aataccctga aaggatgaac cgtcatgtag atgcataatc 600

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 660

601 agtagcgata atggtaccac tacgccaaaa ccgtcatgta gttacccgca cctatcgcca 660

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 720

661 aactgagtgc ccctaaaggt tcagaggtgg ggtaactgca gttaccctca aacaaaaccg 720

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 780

721 tggttttagt tgccctgaaa ggttttacag cattgttgag gcggggtaac tgcgtttacc 780

.......... ......... CMV_imm_pro CMV_f_pri ......... ..........

.......... .......... .......... .......... .......... ..........

781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 840

781 cgccatccgc acatgccacc ctccagatat attcgtcgcg caaaacggac atgacccaga 840

CMV_f_pri CMV_imm_pro ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 900

841 gagaccaatc tggtctagac tcggaccctc gagagaccga ttgatccctt gggtgacgaa 900

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 960

901 ttcggagtta tttcgaacgg aactcacgaa gttcatcaca cacgggcaga caacacactg 960

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... NarI(1019)

961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1020

961 agaccattga tctctaggga gtctgggaaa atcagtcaca ccttttagag atcgtcaccg 1020

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1080

1021 cgggcttgtc cctgaacttt cgctttccct ttggtctcct cgagagagct gcgtcctgag 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1140

1081 ccgaacgact tcgcgcgtgc cgttctccgc tccccgccgc tgaccactca tgcggttttt 1140

.......... .......... .......... ........ HIV-1_psi_pack ........

.......... .......... .......... .......... .......... ..........

1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1200

1141 aaaactgatc gcctccgatc ttcctctctc tacccacgct ctcgcagtca taattcgccc 1200

........ HIV-1_psi_pack ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1260

1201 cctcttaatc tagcgctacc cttttttaag ccaattccgg tccccctttc ttttttatat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1320

1261 ttaattttgt atatcatacc cgttcgtccc tcgatcttgc taagcgtcaa ttaggaccgg 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1380

1321 acaatctttg tagtcttccg acatctgttt atgaccctgt cgatgttggt agggaagtct 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1440

1381 gtcctagtct tcttgaatct agtaatatat tatgtcatcg ttgggagata acacacgtag 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1500

1441 tttcctatct ctattttctg tggttccttc gaaatctgtt ctatctcctt ctcgttttgt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga 1560

1501 tttcattctg gtggcgtgtc gttcgccggc cggcgcgact agaagtctgg acctcctcct 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 1620

1561 ctatactccc tgttaacctc ttcacttaat atatttatat ttcatcattt ttaacttggt 1620

.......... .......... .......... ORF1 .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 1680

1621 aatcctcatc gtgggtggtt ccgtttctct tctcaccacg tctctctttt ttctcgtcac 1680

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 1740

1681 ccttatcctc gaaacaagga acccaagaac cctcgtcgtc cttcgtgata cccgcgtcgc 1740

.......... .......... ORF1 RRE .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 1800

1741 agttactgcg actgccatgt ccggtctgtt aataacagac catatcacgt cgtcgtcttg 1800

.......... .......... ORF1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 1860

1801 ttaaacgact cccgataact ccgcgttgtc gtagacaacg ttgagtgtca gaccccgtag 1860

.......... .......... ORF1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 1920

1861 ttcgtcgagg tccgttctta ggaccgacac ctttctatgg atttcctagt tgtcgaggac 1920

.......... .......... ORF1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 1980

1921 ccctaaaccc caacgagacc ttttgagtaa acgtggtgac gacacggaac cttacgatca 1980

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 2040

1981 acctcattat ttagagacct tgtctaaacc ttagtgtgct ggacctacct caccctgtct 2040

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 2100

2041 ctttaattgt taatgtgttc gaattatgtg aggaattaac ttcttagcgt tttggtcgtt 2100

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 2160

2101 cttttcttac ttgttcttaa taaccttaat ctatttaccc gttcaaacac cttaaccaaa 2160

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 2220

2161 ttgtattgtt taaccgacac catatatttt aataagtatt actatcatcc tccgaaccat 2220

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 2280

2221 ccaaattctt atcaaaaacg acatgaaaga tatcacttat ctcaatccgt ccctataagt 2280

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 2340

2281 ggtaatagca aagtctgggt ggagggttgg ggctcccctg ggctgtccgg gcttccttat 2340

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca 2400

2341 cttcttcttc cacctctctc tctgtctctg tctaggtaag ctaatcactt gcctagccgt 2400

.......... .......... ORF1 .......... .......... ..........

.......... PstI(2420) .......... .......... .......... ..........

2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg 2460

2401 gacgcacgcg gttaagacgt ctgtttaccg tcataagtag gtgttaaaat tttcttttcc 2460

.......... .......... ORF1 .......... .......... cPPT

.......... .......... .......... .......... .......... ..........

2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 2520

2461 cccctaaccc cccatgtcac gtcccctttc ttatcatctg tattatcgtt gtctgtatgt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 2580

2521 ttgatttctt aatgtttttg tttaatgttt ttaagtttta aaagcccaaa taatgtccct 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cagcagagat ccagtttggt tagtaccggg cccggtgctt tgctctgagc cagcccacca 2640

2581 gtcgtctcta ggtcaaacca atcatggccc gggccacgaa acgagactcg gtcgggtggt 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gtttggaatg actccttttt atgacttgaa ttttcaagta taaagtctag tgctaaattt 2700

2641 caaaccttac tgaggaaaaa tactgaactt aaaagttcat atttcagatc acgatttaaa 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aatttgaaca actgtatagt ttttgctggt tgggggaagg aaaaaaaatg gtggcagtgt 2760

2701 ttaaacttgt tgacatatca aaaacgacca acccccttcc ttttttttac caccgtcaca 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ttttttcaga attagaagtg aaatgaaaat tgttgtgtgt gaggatttct aatgacatgt 2820

2761 aaaaaagtct taatcttcac tttactttta acaacacaca ctcctaaaga ttactgtaca 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggtggttgca tactgagtga agccggtgag cattctgcca tgtcaccccc tcgtgctcag 2880

2821 ccaccaacgt atgactcact tcggccactc gtaagacggt acagtggggg agcacgagtc 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 taatgtactt tacagaaatc ctaaactcaa aagattgata taaaccatgc ttcttgtgta 2940

2881 attacatgaa atgtctttag gatttgagtt ttctaactat atttggtacg aagaacacat 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... XbaI(2997)

2941 tatccggtct cttctctggg tagtctcact cagcctgcat ttctgccagg gcccgctcta 3000

2941 ataggccaga gaagagaccc atcagagtga gtcggacgta aagacggtcc cgggcgagat 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gagatccgac gccgccatct ctaggcccgc gccggccccc tcgcacagac ttgtgggaga 3060

3001 ctctaggctg cggcggtaga gatccgggcg cggccggggg agcgtgtctg aacaccctct 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agctcggcta ctcccctgcc ccggttaatt tgcatataat atttcctagt aactatagag 3120

3061 tcgagccgat gaggggacgg ggccaattaa acgtatatta taaaggatca ttgatatctc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcttaatgtg cgataaaaga cagataatct gttcttttta atactagcta cattttacat 3180

3121 cgaattacac gctattttct gtctattaga caagaaaaat tatgatcgat gtaaaatgta 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gataggcttg gatttctata agagatacaa atactaaatt attattttaa aaaacagcac 3240

3181 ctatccgaac ctaaagatat tctctatgtt tatgatttaa taataaaatt ttttgtcgtg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt tgagactata aatatccctt 3300

3241 ttttcctttg agtgggattg acatttcatt aacacacaaa actctgatat ttatagggaa 3300

.......... .......... .......... .......... .......... ..........

.......... HpaI(3316) .......... .......... .......... ..........

3301 ggagaaaagc cttgttaacg cgcggtgacc ctcgaggtcg acggtatcga taagctcgct 3360

3301 cctcttttcg gaacaattgc gcgccactgg gagctccagc tgccatagct attcgagcga 3360

.......... .......... ......... pBluesKS_pri ......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcacgagatt ccagcaggtc gagggaccta ataacttcgt atagcataca ttatacgaag 3420

3361 agtgctctaa ggtcgtccag ctccctggat tattgaagca tatcgtatgt aatatgcttc 3420

.......... .......... .......... .......... loxP ..........

.......... .......... NotI(3446) .......... .......... ..........

3421 ttatattaag ggttccaagc ttaagcggcc gcgtggataa ccgtattacc gccatgcatt 3480

3421 aatataattc ccaaggttcg aattcgccgg cgcacctatt ggcataatgg cggtacgtaa 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 3540

3481 tcaataatta tcattagtta atgccccagt aatcaagtat cgggtatata cctcaaggcg 3540

.......... .......... ......... CMV_imm_pro ......... ..........

.......... .......... .......... .......... .......... ..........

3541 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 3600

3541 caatgtattg aatgccattt accgggcgga ccgactggcg ggttgctggg ggcgggtaac 3600

.......... ......... CMV_imm_pro CAG_en ......... ..........

.......... .......... .......... .......... .......... ..........

3601 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 3660

3601 tgcagttatt actgcataca agggtatcat tgcggttatc cctgaaaggt aactgcagtt 3660

.......... ...... CMV_imm_pro CAG_en ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 3720

3661 acccacctca taaatgccat ttgacgggtg aaccgtcatg tagttcacat agtatacggt 3720

.......... ...... CMV_imm_pro CAG_en ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 3780

3721 tcatgcgggg gataactgca gttactgcca tttaccgggc ggaccgtaat acgggtcatg 3780

.......... ...... CMV_imm_pro CAG_en ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 3840

3781 tactggaata ccctgaaagg atgaaccgtc atgtagatgc ataatcagta gcgataatgg 3840

.......... ...... CMV_imm_pro CAG_en ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 3900

3841 taccactacg ccaaaaccgt catgtagtta cccgcaccta tcgccaaact gagtgcccct 3900

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 3960

3901 aaaggttcag aggtggggta actgcagtta ccctcaaaca aaaccgtggt tttagttgcc 3960

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 4020

3961 ctgaaaggtt ttacagcatt gttgaggcgg ggtaactgcg tttacccgcc atccgcacat 4020

.......... ......... CMV_imm_pro ..... CMV_f_pri CMV_pro ......

.......... .......... .......... .......... NheI(4070) AgeI(4079)

4021 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 4080

4021 gccaccctcc agatatattc gtctcgacca aatcacttgg cagtctaggc gatcgcgatg 4080

CMV_f_pri CMV_imm_pro CMV_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 4140

4081 gccagcggtg gtaccactcg ttcccgctcc tcgacaagtg gccccaccac gggtaggacc 4140

.......... .......... ORF3 EGFP ORF3 .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 4200

4141 agctcgacct gccgctgcat ttgccggtgt tcaagtcgca caggccgctc ccgctcccgc 4200

EGFP_N_pri ........ ORF3 EGFP ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 4260

4201 tacggtggat gccgttcgac tgggacttca agtagacgtg gtggccgttc gacgggcacg 4260

.......... ........ ORF3 EGFP ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 4320

4261 ggaccgggtg ggagcactgg tgggactgga tgccgcacgt cacgaagtcg gcgatggggc 4320

.......... ........ ORF3 EGFP ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 4380

4321 tggtgtactt cgtcgtgctg aagaagttca ggcggtacgg gcttccgatg caggtcctcg 4380

.......... ........ ORF3 EGFP ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 4440

4381 cgtggtagaa gaagttcctg ctgccgttga tgttctgggc gcggctccac ttcaagctcc 4440

.......... ........ ORF3 EGFP ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 4500

4441 cgctgtggga ccacttggcg tagctcgact tcccgtagct gaagttcctc ctgccgttgt 4500

.......... ........ ORF3 EGFP ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 4560

4501 aggaccccgt gttcgacctc atgttgatgt tgtcggtgtt gcagatatag taccggctgt 4560

.......... ........ ORF3 EGFP ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 4620

4561 tcgtcttctt gccgtagttc cacttgaagt tctaggcggt gttgtagctc ctgccgtcgc 4620

.......... ........ ORF3 EGFP ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 4680

4621 acgtcgagcg gctggtgatg gtcgtcttgt gggggtagcc gctgccgggg cacgacgacg 4680

.......... ........ ORF3 EGFP ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 4740

4681 ggctgttggt gatggactcg tgggtcaggc gggactcgtt tctggggttg ctcttcgcgc 4740

.......... ........ ORF3 EGFP ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 4800

4741 tagtgtacca ggacgacctc aagcactggc ggcggcccta gtgagagccg tacctgctcg 4800

........ EGFP_C_pri ORF3 EGFP ORF3 ........ .......... ..........

......... EcoRI(4812) ......... .......... .......... ..........

4801 tgtacaagta ggaattcgtc gagggaccta ataacttcgt atagcataca ttatacgaag 4860

4801 acatgttcat ccttaagcag ctccctggat tattgaagca tatcgtatgt aatatgcttc 4860

ORF3 .......... ORF3 .......... loxP ..........

.......... .......... .......... .......... .......... ..........

4861 ttatacatgt ttaagggttc cggttccact aggtacaatt cgatatcaag cttatcgata 4920

4861 aatatgtaca aattcccaag gccaaggtga tccatgttaa gctatagttc gaatagctat 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 4980

4921 tagttggaga cctaatgttt taaacacttt ctaactgacc ataagaattg atacaacgag 4980

.......... .......... .......... WPRE .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 5040

4981 gaaaatgcga tacacctatg cgacgaaatt acggaaacat agtacgataa cgaagggcat 5040

.......... .......... WPRE .......... .......... ORF1

.......... .......... .......... .......... .......... ..........

5041 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 5100

5041 accgaaagta aaagaggagg aacatattta ggaccaacga cagagaaata ctcctcaaca 5100

.......... .......... WPRE ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 5160

5101 ccgggcaaca gtccgttgca ccgcaccaca cgtgacacaa acgactgcgt tgggggtgac 5160

.......... .......... WPRE ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 5220

5161 caaccccgta acggtggtgg acagtcgagg aaaggccctg aaagcgaaag ggggagggat 5220

.......... .......... WPRE ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 5280

5221 aacggtgccg ccttgagtag cggcggacgg aacgggcgac gacctgtccc cgagccgaca 5280

.......... .......... WPRE ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 5340

5281 acccgtgact gttaaggcac cacaacagcc cctttagtag caggaaagga accgacgagc 5340

.......... .......... WPRE ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 5400

5341 ggacacaacg gtggacctaa gacgcgccct gcaggaagac gatgcaggga agccgggagt 5400

.......... .......... WPRE ORF1 .......... .......... ..........

.......... ......... SacII(5423) ......... .......... ..........

5401 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 5460

5401 taggtcgcct ggaaggaagg gcgccggacg acggccgaga cgccggagaa ggcgcagaag 5460

.......... .......... WPRE ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc 5520

5461 cggaagcggg agtctgctca gcctagaggg aaacccggcg gaggggcgta gctatggcag 5520

.......... .......... WPRE ORF1 .......... ......... pBluesKS_pri

.......... .......... .......... .......... .......... ..........

5521 gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 5580

5521 ctggagctag ctctggatct ttttgtacct cgttagtgtt catcgttatg tcgtcgatgg 5580

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 5640

5581 ttacgactaa cacggaccga tcttcgtgtt ctcctcctcc tccacccaaa aggtcagtgt 5640

.......... .......... ORF1 .......... .......... ..........

KpnI(5650) .......... .......... .......... .......... ..........

5641 cctcaggtac caagcatggg gtaaagtact gttctcatca catcatatca aggttatata 5700

5641 ggagtccatg gttcgtaccc catttcatga caagagtagt gtagtatagt tccaatatat 5700

.......... ORF1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ccatcaatat tgccacagat gttacttagc cttttaatat ttctctaatt tagtgtatat 5760

5701 ggtagttata acggtgtcta caatgaatcg gaaaattata aagagattaa atcacatata 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gcaatgatag ttctctgatt tctgagattg agtttctcat gtgtaatgat tatttagagt 5820

5761 cgttactatc aagagactaa agactctaac tcaaagagta cacattacta ataaatctca 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ttctctttca tctgttcaaa tttttgtcta gttttatttt ttactgattt gtaagacttc 5880

5821 aagagaaagt agacaagttt aaaaacagat caaaataaaa aatgactaaa cattctgaag 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tttttataat ctgcatatta caattctctt tactggggtg ttgcaaatat tttctgtcat 5940

5881 aaaaatatta gacgtataat gttaagagaa atgaccccac aacgtttata aaagacagta 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tctatggcct gacttttctt aatggttttt taattttaaa aataagtctt aatattcatg 6000

5941 agataccgga ctgaaaagaa ttaccaaaaa attaaaattt ttattcagaa ttataagtac 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 caatctaatt aacaatcttt tctttgtggt taggactttg agtcataaga aatttttctc 6060

6001 gttagattaa ttgttagaaa agaaacacca atcctgaaac tcagtattct ttaaaaagag 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tacactgaag tcatgatggc atgcttctat attattttct aaaagattta aagttttgcc 6120

6061 atgtgacttc agtactaccg tacgaagata taataaaaga ttttctaaat ttcaaaacgg 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ttctccattt agacttataa ttcactggaa tttttttgtg tgtatggtat gacatatggg 6180

6121 aagaggtaaa tctgaatatt aagtgacctt aaaaaaacac acataccata ctgtataccc 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ttccctttta ttttttacat ataaatatat ttccctgttt ttctaaaaaa gaaaaagatc 6240

6181 aagggaaaat aaaaaatgta tatttatata aagggacaaa aagatttttt ctttttctag 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 atcattttcc cattgtaaaa tgccatattt ttttcatagg tcacttacat atatcaatgg 6300

6241 tagtaaaagg gtaacatttt acggtataaa aaaagtatcc agtgaatgta tatagttacc 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gtctgtttct gagctctact ctattttatc agcctcactg tctatcccca cacatctcat 6360

6301 cagacaaaga ctcgagatga gataaaatag tcggagtgac agataggggt gtgtagagta 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gctttgctct aaatcttgat atttagtgga acattctttc ccattttgtt ctacaagaat 6420

6361 cgaaacgaga tttagaacta taaatcacct tgtaagaaag ggtaaaacaa gatgttctta 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 atttttgtta ttgtcttttg ggcttctata tacattttag aatgaggttg gcaagttctg 6480

6421 taaaaacaat aacagaaaac ccgaagatat atgtaaaatc ttactccaac cgttcaagac 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 6540

6481 atctagaatc ggtgaaaaat tttcttttcc cccctgacct tcccgattaa gtgagggttg 6540

.......... .......... cPPT U3PPT .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc 6600

6541 cttctgttct ataggaacta gacacctaga tggtgtgtgt tccgatgaag ggactaaccg 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc 6660

6601 tcttgatgtg tggtcccggt ccctagtcta taggtgactg gaaacctacc acgatgttcg 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt 6720

6661 atcatggtca actcgttctc ttccatcttc ttcggttact tcctctcttg tgggcgaaca 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt 6780

6721 atgtgggaca ctcggacgta ccctacctac tgggcctctc tcttcataat ctcacctcca 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt 6840

6781 aactgtcggc ggatcgtaaa gtagtgtacc gggctctcga cgtaggcctg acatgaccca 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6900

6841 gagagaccaa tctggtctag actcggaccc tcgagagacc gattgatccc ttgggtgacg 6900

.......... ......... truncHIV HIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 6960

6901 aattcggagt tatttcgaac ggaactcacg aagttcatca cacacgggca gacaacacac 6960

.......... ......... truncHIV HIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca 7020

6961 tgagaccatt gatctctagg gagtctggga aaatcagtca caccttttag agatcgtcgt 7020

.......... ......... truncHIV HIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 7080

7021 acactcgttt tccggtcgtt ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa 7080

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

7081 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 7140

7081 aggtatccga ggcgggggga ctgctcgtag tgtttttagc tgcgagttca gtctccaccg 7140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7141 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 7200

7141 ctttgggctg tcctgatatt tctatggtcc gcaaaggggg accttcgagg gagcacgcga 7200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 7260

7201 gaggacaagg ctgggacggc gaatggccta tggacaggcg gaaagaggga agcccttcgc 7260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 7320

7261 accgcgaaag agtatcgagt gcgacatcca tagagtcaag ccacatccag caagcgaggt 7320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7321 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 7380

7321 tcgacccgac acacgtgctt ggggggcaag tcgggctggc gacgcggaat aggccattga 7380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7381 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 7440

7381 tagcagaact caggttgggc cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat 7440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7441 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 7500

7441 tgtcctaatc gtctcgctcc atacatccgc cacgatgtct caagaacttc accaccggat 7500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7501 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 7560

7501 tgatgccgat gtgatcttct tgtcataaac catagacgcg agacgacttc ggtcaatgga 7560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7561 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 7620

7561 agcctttttc tcaaccatcg agaactaggc cgtttgtttg gtggcgacca tcgccaccaa 7620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7621 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 7680

7621 aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc tagagttctt ctaggaaact 7680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7681 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 7740

7681 agaaaagatg ccccagactg cgagtcacct tgcttttgag tgcaattccc taaaaccagt 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 7800

7741 actctaatag tttttcctag aagtggatct aggaaaattt aatttttact tcaaaattta 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 7860

7801 gttagatttc atatatactc atttgaacca gactgtcaat ggttacgaat tagtcactcc 7860

.......... .......... .......... ....... ORF2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

7861 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 7920

7861 gtggatagag tcgctagaca gataaagcaa gtaggtatca acggactgag gggcagcaca 7920

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 7980

7921 tctattgatg ctatgccctc ccgaatggta gaccggggtc acgacgttac tatggcgctc 7980

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 8040

7981 tgggtgcgag tggccgaggt ctaaatagtc gttatttggt cggtcggcct tcccggctcg 8040

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 8100

8041 cgtcttcacc aggacgttga aataggcgga ggtaggtcag ataattaaca acggcccttc 8100

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... FspI(8133) .......... ..........

8101 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 8160

8101 gatctcattc atcaagcggt caattatcaa acgcgttgca acaacggtaa cgatgtccgt 8160

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 8220

8161 agcaccacag tgcgagcagc aaaccatacc gaagtaagtc gaggccaagg gttgctagtt 8220

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 8280

8221 ccgctcaatg tactaggggg tacaacacgt tttttcgcca atcgaggaag ccaggaggct 8280

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 8340

8281 agcaacagtc ttcattcaac cggcgtcaca atagtgagta ccaataccgt cgtgacgtat 8340

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 8400

8341 taagagaatg acagtacggt aggcattcta cgaaaagaca ctgaccactc atgagttggt 8400

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 8460

8401 tcagtaagac tcttatcaca tacgccgctg gctcaacgag aacgggccgc agttatgccc 8460

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 8520

8461 tattatggcg cggtgtatcg tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc 8520

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 8580

8521 ccgcttttga gagttcctag aatggcgaca actctaggtc aagctacatt gggtgagcac 8580

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 8640

8581 gtgggttgac tagaagtcgt agaaaatgaa agtggtcgca aagacccact cgtttttgtc 8640

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 8700

8641 cttccgtttt acggcgtttt ttcccttatt cccgctgtgc ctttacaact tatgagtatg 8700

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 8760

8701 agaaggaaaa agttataata acttcgtaaa tagtcccaat aacagagtac tcgcctatgt 8760

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

8761 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 8820

8761 ataaacttac ataaatcttt ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc 8820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 tgccacctga c......... .......... .......... .......... .......... 8831

8821 acggtggact g......... .......... .......... .......... .......... 8831

.......... .......... .......... .......... .......... ..........