Sequence of pLI50

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Sequence of pLI50

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Sequence of pLI50 : 5505 BP; 1735 A; 1012 C; 1183 G; 1575 T; 0 other;

EcoRI SacI KpnI,SmaI,BamHI XbaI SalI) HindIII(52)

1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttctg 60

1 cttaagctcg agccatgggc ccctaggaga tctcagctgg acgtccgtac gttcgaagac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 taggttttta ggcataaaac tatatgattt acccctaaat ctttaaaatg ccccttaaaa 120

61 atccaaaaat ccgtattttg atatactaaa tggggattta gaaattttac ggggaatttt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttcaaaataa aggcatttaa aatttaaata tttcttgtga taaagtttgt taaaaaggag 180

121 aagttttatt tccgtaaatt ttaaatttat aaagaacact atttcaaaca atttttcctc 180

.......... .......... .......... .......... .......... ..........

.......... .......... ClaI(206) .......... .......... ..........

181 tggttttatg actgttatgt ggttatcgat tataggtatg tggttttgta ttggaatggc 240

181 accaaaatac tgacaataca ccaatagcta atatccatac accaaaacat aaccttaccg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 attttttgct atcaaggtta ttaaaaataa aaattagacc acgcatttat gccgagaaaa 300

241 taaaaaacga tagttccaat aatttttatt tttaatctgg tgcgtaaata cggctctttt 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tttattgtgc gttgagaaga acccttaact aaacttgcag acgaatgtcg gcatagcgtg 360

301 aaataacacg caactcttct tgggaattga tttgaacgtc tgcttacagc cgtatcgcac 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 agctattaag ccgaccattc gacaagtttt gggattgtta agggttccga ggctcaacgt 420

361 tcgataattc ggctggtaag ctgttcaaaa ccctaacaat tcccaaggct ccgagttgca 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caataaagca attggaataa agaagcgaaa aaggagaagt cggttcagaa aaagaaggat 480

421 gttatttcgt taaccttatt tcttcgcttt ttcctcttca gccaagtctt tttcttccta 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atggatctgg agctgtaata taaaaacctt cttcaactaa cggggcaggt tagtgacatt 540

481 tacctagacc tcgacattat atttttggaa gaagttgatt gccccgtcca atcactgtaa 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 agaaaaccga ctgtaaaaag tacagtcggc attatctcat attataaaag ccagtcatta 600

541 tcttttggct gacatttttc atgtcagccg taatagagta taatattttc ggtcagtaat 600

.......... .......... .......... .......... ......... Orf frame 3

StuI(602) .......... .......... .......... .......... ..........

601 ggcctatctg acaattcctg aatagagttc ataaacaatc ctgcatgata accatcacaa 660

601 ccggatagac tgttaaggac ttatctcaag tatttgttag gacgtactat tggtagtgtt 660

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 acagaatgat gtacctgtaa agatagcggt aaatatattg aattaccttt attaatgaat 720

661 tgtcttacta catggacatt tctatcgcca tttatataac ttaatggaaa taattactta 720

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tttcctgctg taataatggg tagaaggtaa ttactattat tattgatatt taagttaaac 780

721 aaaggacgac attattaccc atcttccatt aatgataata ataactataa attcaatttg 780

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccagtaaatg aagtccatgg aataatagaa agagaaaaag cattttcagg tataggtgtt 840

781 ggtcatttac ttcaggtacc ttattatctt tctctttttc gtaaaagtcc atatccacaa 840

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttgggaaaca atttccccga accattatat ttctctacat cagaaaggta taaatcataa 900

841 aaccctttgt taaaggggct tggtaatata aagagatgta gtctttccat atttagtatt 900

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aactctttga agtcattctt tacaggagtc caaataccag agaatgtttt agatacacca 960

901 ttgagaaact tcagtaagaa atgtcctcag gtttatggtc tcttacaaaa tctatgtggt 960

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tcaaaaattg tataaagtgg ctctaactta tcccaataac ctaactctcc gtcgctattg 1020

961 agtttttaac atatttcacc gagattgaat agggttattg gattgagagg cagcgataac 1020

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 taaccagttc taaaagctgt atttgagttt atcacccttg tcactaagaa aataaatgca 1080

1021 attggtcaag attttcgaca taaactcaaa tagtgggaac agtgattctt ttatttacgt 1080

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gggtaaaatt tatatccttc ttgttttatg tttcggtata aaacactaat atcaatttct 1140

1081 cccattttaa atataggaag aacaaaatac aaagccatat tttgtgatta tagttaaaga 1140

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtggttatac taaaagtcgt ttgttggttc aaataatgat taaatatctc ttttctcttc 1200

1141 caccaatatg attttcagca aacaaccaag tttattacta atttatagag aaaagagaag 1200

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 caattgtcta aatcaatttt attaaagttc atttgatatg cctcctaaat ttttatctaa 1260

1201 gttaacagat ttagttaaaa taatttcaag taaactatac ggaggattta aaaatagatt 1260

......... Orf frame 3 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 agtgaattta ggaggcttac ttgtctgctt tcttcattag aatcaatcct ttttttaaaa 1320

1261 tcacttaaat cctccgaatg aacagacgaa agaagtaatc ttagttagga aaaaaatttt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat ccaattttcg 1380

1321 cagttataat gacattgtat ttatatataa aatttttata gggtgaaata ggttaaaagc 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tttgttgaat ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat 1440

1381 aaacaactta aaaaaatttt cagttataat gacattgtat ttatatataa aatttttata 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaaaga 1500

1441 gggtgaaata ggttaaaagc aaacaacttg attacccacg aaatcaactt cttattttct 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ccacattaaa aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc 1560

1501 ggtgtaattt tttacaccag aaaacacaaa aaaatttcct aaactcgcat cgctttttag 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cttttctttc ttatcttgat acatatagaa ataacgtcat ttttatttta gttgctgaaa 1620

1561 gaaaagaaag aatagaacta tgtatatctt tattgcagta aaaataaaat caacgacttt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt 1680

1621 ccacgcaact tcacaaccat acatacacaa aatttcataa cttttgggaa ttttaaccaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcacagaaaa accccatctg ttaaagttat aagtgactaa acaaataact aaatagatgg 1740

1681 cgtgtctttt tggggtagac aatttcaata ttcactgatt tgtttattga tttatctacc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gggtttcttt taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg 1800

1741 cccaaagaaa attataatac acaggattat catcgtaaat aagtctactt tttagttccc 1800

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttttagtgga caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta 1860

1801 aaaatcacct gttctgtttt tcaccttttc actctggtac ctctcttttc ttttagcgat 1860

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atgttgatta ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag 1920

1861 tacaactaat gaaacttgaa gacgtataag aacttaaatt tttccgactt tctcattttc 1920

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 attgtgctga aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc 1980

1921 taacacgact ttataatctc atatttgttt tagcactttg tccgctttct ttcaacatag 1980

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac 2040

1981 ctcacaccaa aacatttagg tccgaaacag gttacacgtt gacctcctct cgttactttg 2040

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 atggcattca gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt 2100

2041 taccgtaagt cagtgttttc caacaacgac ttcaataatt tgttttcggt tgtcaagcaa 2100

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggttgtttct cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt 2160

2101 ccaacaaaga gtgtaattgt caatttttac aaatactacc gcttcttaat ttattctcaa 2160

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgtcagatat ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatc 2220

2161 acagtctata ccgagttcct aaagcggctt actacgttat atttttttaa ttatttttag 2220

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttgttggttt tatgcgtgca acggaagtga caataaataa taaagataat tcttataatc 2280

2221 aacaaccaaa atacgcacgt tgccttcact gttatttatt atttctatta agaatattag 2280

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 agcacatgca tgtattggta tgtgtggaac caacttattt taagaataca gaaaactacg 2340

2281 tcgtgtacgt acataaccat acacaccttg gttgaataaa attcttatgt cttttgatgc 2340

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tgaatcaaaa acaatggatt caattttgga aaaaggcaat gaaattagac tatgatccaa 2400

2341 acttagtttt tgttacctaa gttaaaacct ttttccgtta ctttaatctg atactaggtt 2400

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa atcggatata caatcggcaa 2460

2401 tacattttca agtttactaa gctggctttt tatttatatt tagcctatat gttagccgtt 2460

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttgacgaaac tgcaaaatat cctgtaaagg atacggattt tatgaccgat gatgaagaaa 2520

2461 aactgctttg acgttttata ggacatttcc tatgcctaaa atactggcta ctacttcttt 2520

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agaatttgaa acgtttgtct gatttggagg aaggtttaca ccgtaaaagg ttaatctcct 2580

2521 tcttaaactt tgcaaacaga ctaaacctcc ttccaaatgt ggcattttcc aattagagga 2580

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atggtggttt gttaaaagaa atacataaaa aattaaacct tgatgacaca gaagaaggcg 2640

2581 taccaccaaa caattttctt tatgtatttt ttaatttgga actactgtgt cttcttccgc 2640

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atttgattca tacagatgat gacgaaaaag ccgatgaaga tggattttct attattgcaa 2700

2641 taaactaagt atgtctacta ctgctttttc ggctacttct acctaaaaga taataacgtt 2700

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgtggaattg ggaacggaaa aattatttta ttaaagagta gttcaacaaa cgggccagtt 2760

2701 acaccttaac ccttgccttt ttaataaaat aatttctcat caagttgttt gcccggtcaa 2760

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgttgaagat tagatgctat aattgttatt aaaaggattg aaggatgctt aggaagacga 2820

2761 acaacttcta atctacgata ttaacaataa ttttcctaac ttcctacgaa tccttctgct 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gttattaata gctgaataag aacggtgctc tccaaatatt cttatttaga aaagcaaatc 2880

2821 caataattat cgacttattc ttgccacgag aggtttataa gaataaatct tttcgtttag 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 taaaattatc tgaaaaggga atgagaatag tgaatggacc aataataatg actagagaag 2940

2881 attttaatag acttttccct tactcttatc acttacctgg ttattattac tgatctcttc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aaagaatgaa gattgttcat gaaattaagg aacgaatatt ggataaatat ggggatgatg 3000

2941 tttcttactt ctaacaagta ctttaattcc ttgcttataa cctatttata cccctactac 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ApaI(3049) ..........

3001 ttaaggctat tggtgtttat ggctctcttg gtcgtcagac tgatgggccc tattcggata 3060

3001 aattccgata accacaaata ccgagagaac cagcagtctg actacccggg ataagcctat 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttgagatgat gtgtgtcatg tcaacagagg aagcagagtt cagccatgaa tggacaaccg 3120

3061 aactctacta cacacagtac agttgtctcc ttcgtctcaa gtcggtactt acctgttggc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gaaagagcga aaatgcctca catttgtgcc acctaaaaag gagcgattta catatgagtt 3180

3121 ctttctcgct tttacggagt gtaaacacgg tggatttttc ctcgctaaat gtatactcaa 3180

.......... .......... .......... .......... .......... ..........

.......... .......... ......... PvuII(3213) ......... ..........

3181 atgcagtttg tagaatgcaa aaagtgaaat cagctgcctc gcgcgtttcg gtgatgacgg 3240

3181 tacgtcaaac atcttacgtt tttcacttta gtcgacggag cgcgcaaagc cactactgcc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 3300

3241 acttttggag actgtgtacg tcgagggcct ctgccagtgt cgaacagaca ttcgcctacg 3300

........ pGEX_3_primer ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 3360

3301 gccctcgtct gttcgggcag tcccgcgcag tcgcccacaa ccgcccacag ccccgcgtcg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag 3420

3361 gtactgggtc agtgcatcgc tatcgcctca catatgaccg aattgatacg ccgtagtctc 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 3480

3421 gtctaacatg actctcacgt ggtatacgcc acactttatg gcgtgtctac gcattcctct 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 3540

3481 tttatggcgt agtccgcgag aaggcgaagg agcgagtgac tgagcgacgc gagccagcaa 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 3600

3541 gccgacgccg ctcgccatag tcgagtgagt ttccgccatt atgccaatag gtgtcttagt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 3660

3601 cccctattgc gtcctttctt gtacactcgt tttccggtcg ttttccggtc cttggcattt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 3720

3661 ttccggcgca acgaccgcaa aaaggtatcc gaggcggggg gactgctcgt agtgttttta 3720

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3721 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 3780

3721 gctgcgagtt cagtctccac cgctttgggc tgtcctgata tttctatggt ccgcaaaggg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 3840

3781 ggaccttcga gggagcacgc gagaggacaa ggctgggacg gcgaatggcc tatggacagg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 3900

3841 cggaaagagg gaagcccttc gcaccgcgaa agagtatcga gtgcgacatc catagagtca 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 3960

3901 agccacatcc agcaagcgag gttcgacccg acacacgtgc ttggggggca agtcgggctg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 4020

3961 gcgacgcgga ataggccatt gatagcagaa ctcaggttgg gccattctgt gctgaatagc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 4080

4021 ggtgaccgtc gtcggtgacc attgtcctaa tcgtctcgct ccatacatcc gccacgatgt 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 4140

4081 ctcaagaact tcaccaccgg attgatgccg atgtgatctt cctgtcataa accatagacg 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 4200

4141 cgagacgact tcggtcaatg gaagcctttt tctcaaccat cgagaactag gccgtttgtt 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 4260

4201 tggtggcgac catcgccacc aaaaaaacaa acgttcgtcg tctaatgcgc gtcttttttt 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 4320

4261 cctagagttc ttctaggaaa ctagaaaaga tgccccagac tgcgagtcac cttgcttttg 4320

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 4380

4321 agtgcaattc cctaaaacca gtactctaat agtttttcct agaagtggat ctaggaaaat 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 4440

4381 ttaattttta cttcaaaatt tagttagatt tcatatatac tcatttgaac cagactgtca 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 4500

4441 atggttacga attagtcact ccgtggatag agtcgctaga cagataaagc aagtaggtat 4500

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 4560

4501 caacggactg aggggcagca catctattga tgctatgccc tcccgaatgg tagaccgggg 4560

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 4620

4561 tcacgacgtt actatggcgc tctgggtgcg agtggccgag gtctaaatag tcgttatttg 4620

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 4680

4621 gtcggtcggc cttcccggct cgcgtcttca ccaggacgtt gaaataggcg gaggtaggtc 4680

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... FspI(4735)

4681 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 4740

4681 agataattaa caacggccct tcgatctcat tcatcaagcg gtcaattatc aaacgcgttg 4740

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 4800

4741 caacaacggt aacgacgtcc gtagcaccac agtgcgagca gcaaaccata ccgaagtaag 4800

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 4860

4801 tcgaggccaa gggttgctag ttccgctcaa tgtactaggg ggtacaacac gttttttcgc 4860

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 4920

4861 caatcgagga agccaggagg ctagcaacag tcttcattca accggcgtca caatagtgag 4920

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 4980

4921 taccaatacc gtcgtgacgt attaagagaa tgacagtacg gtaggcattc tacgaaaaga 4980

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 5040

4981 cactgaccac tcatgagttg gttcagtaag actcttatca catacgccgc tggctcaacg 5040

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 5100

5041 agaacgggcc gcagttgtgc cctattatgg cgcggtgtat cgtcttgaaa ttttcacgag 5100

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 5160

5101 tagtaacctt ttgcaagaag ccccgctttt gagagttcct agaatggcga caactctagg 5160

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 5220

5161 tcaagctaca ttgggtgagc acgtgggttg actagaagtc gtagaaaatg aaagtggtcg 5220

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 5280

5221 caaagaccca ctcgtttttg tccttccgtt ttacggcgtt ttttccctta ttcccgctgt 5280

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 5340

5281 gcctttacaa cttatgagta tgagaaggaa aaagttataa taacttcgta aatagtccca 5340

Orf frame 3 Ampicillin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 5400

5341 ataacagagt actcgcctat gtataaactt acataaatct ttttatttgt ttatccccaa 5400

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... ......... AatII(5435) ......... ..........

5401 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 5460

5401 ggcgcgtgta aaggggcttt tcacggtgga ctgcagattc tttggtaata atagtactgt 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaa..... .......... 5505

5461 aattggatat ttttatccgc atagtgctcc gggaaagcag aagtt..... .......... 5505

.......... .......... .......... .......... .......... ..........