Alternative names: LKO.1
Insert size (bp): Unknown
Vector backbone: pLKO.1
Type of vector: Mammalian expression,Lentiviral,RNAi
Backbone size (bp): 7032
Cloning site 5': AgeI
Site destroyed during cloning: No
Cloning site 3': EcoRI
Site destroyed during cloning: No
5' Sequencing primer: LKO.1 5'
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Selectable markers: Puromycin
Plasmid Provided In: DH5a
Principal Investigator: Bob Weinberg
Comments: Empty lentiviral vector for siRNA expression; replaces Lentihair.
Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart SA et al. (RNA 2003 Apr;9(4):493-501. Pubmed)