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pLKO.3G
Gene/insert name: none
Insert size (bp): Unknown
Vector backbone: pLKO.3G
Type of vector: Mammalian expression, RNAi
Backbone size (bp): 8174
Cloning site 5': EcoRI
Site destroyed during cloning: No
Cloning site 3': PacI
Site destroyed during cloning: No
5' Sequencing primer: LKO.1 5'
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Selectable markers: eGFP
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: Derived from the pLKO.1 plasmid from The RNAi Consortium (TRC)
Plasmid Provided In: Stbl3
Principal Investigator: Christophe Benoist, Diane Mathis
Comments: A variant of pLKO.1 containing an eGFP marker rather than puromycin. Please note that the AgeI site is NOT unique for this vector, so shRNAs should be designed for cloning with EcoRI and PacI instead. Forward oligo: 5'-AATT—21bp sense—CTCGAG—21bp antisense—TTTTTTTAT-3'. Reverse oligo: 5'-AAAAAAA—21bp sense—CTCGAG—21bp antisense-3'.
pLKO.3G was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with eGFP from another vector using the enzymes BamHI and BsRGI.