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    Plasmid map of pLKO

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    Gene/insert name: none
    Insert size (bp): Unknown
    Vector backbone: pLKO.3G
    Type of vector: Mammalian expression, RNAi
    Backbone size (bp): 8174
    Cloning site 5': EcoRI
    Site destroyed during cloning: No
    Cloning site 3': PacI
    Site destroyed during cloning: No
    5' Sequencing primer: LKO.1 5'
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Selectable markers: eGFP
    If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: Derived from the pLKO.1 plasmid from The RNAi Consortium (TRC)
    Plasmid Provided In: Stbl3
    Principal Investigator: Christophe Benoist, Diane Mathis
    Comments: A variant of pLKO.1 containing an eGFP marker rather than puromycin. Please note that the AgeI site is NOT unique for this vector, so shRNAs should be designed for cloning with EcoRI and PacI instead. Forward oligo: 5'-AATT—21bp sense—CTCGAG—21bp antisense—TTTTTTTAT-3'. Reverse oligo: 5'-AAAAAAA—21bp sense—CTCGAG—21bp antisense-3'.
    pLKO.3G was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with eGFP from another vector using the enzymes BamHI and BsRGI.

    image of pLKO

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