Sequence of pLKO

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pLKO

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pLKO.3G : 8174 BP; 2100 A; 2026 C; 2122 G; 1926 T; 0 other;

NdeI(2) .......... .......... .......... .......... ..........

1 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa 60

1 gtatacgaat ggcattgaac tttcataaag ctaaagaacc gaaatatata gaacaccttt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... EcoRI(94) .......... ..........

61 ggacgaaaca ccggtccgca ggtatgcttg cgtgaattcc acctcgagct acttaattaa 120

61 cctgctttgt ggccaggcgt ccatacgaac gcacttaagg tggagctcga tgaattaatt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 accagttttc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg 180

121 tggtcaaaag ctggagctct gtttaccgtc ataagtaggt gttaaaattt tcttttcccc 180

.......... .......... .......... .......... .......... cPPT

.......... .......... .......... .......... .......... ..........

181 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa 240

181 cctaaccccc catgtcacgt cccctttctt atcatctgta ttatcgttgt ctgtatgttt 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca 300

241 gatttcttaa tgtttttgtt taatgttttt aagttttaaa agcccaaata atgtccctgt 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc 360

301 cgtctctagg tgaaaccggc gccgagctcc cccaacccca acgcggaaaa ggttccgtcg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc 420

361 ggacccaaac gcgtccctgc gccgacgaga cccgcaccaa ggccctttgc gtcgccgcgg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct 480

421 ctgggaccca gagcgtgtaa gaagtgcagg caagcgtcgc agtgggccta gaagcggcga 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg 540

481 tgggaacacc cggggggccg ctgcgaagga cgaggcgggg attcagccct tccaaggaac 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca 600

541 gccaagcgcc gcacggcctg cactgtttgc cttcggcgtg cagagtgatc atgggagcgt 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gacggacagc gccagggagc aatggcagcg cgccgaccgc ggaagccgca cgtctcacta 660

601 ctgcctgtcg cggtccctcg ttaccgtcgc gcggctggcg ccttcggcgt gcagagtgat 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg 720

661 catgggagcg tctgcctgtc gcggtccctc gttaccgtcg cgcggctggc gctacccgac 720

.......... .......... .......... .......... ORF3 ..........

MscI(723) .......... .......... .......... .......... ..........

721 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc 780

721 accggttatc gccgacgagt cgtcccgcgc ggctctcgtc gccggccctt ccccgccacg 780

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat 840

781 ccctccgccc cacaccccgc catcacaccc gggacaagga cgggcgcgcc acaaggcgta 840

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc 900

841 agacgttcgg aggcctcgcg tgcagccgtc agccgaggga gcaactggct tagtggctgg 900

.......... .......... ORF3 .......... .......... ..........

.......... BamHI(914) .......... .......... .......... ..........

901 tctctcccca gggggatcca ccggtcgcca ccatggtgag caagggcgag ga gctgttca 960

901 agagaggggt ccccctaggt ggccagcggt ggtaccactc gttcccgctc ctcgacaagt 960

.......... .......... ORF3 .......... EGFP ..........

.......... .......... .......... .......... .......... ..........

961 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 1020

961 ggccccacca cgggtaggac cagctcgacc tgccgctgca tttgccggtg ttcaagtcgc 1020

.......... ....... ORF3 EGFP EGFP_N ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 1080

1021 acaggccgct cccgctcccg ctacggtgga tgccgttcga ctgggacttc aagtagacgt 1080

.......... .......... ORF3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 1140

1081 ggtggccgtt cgacgggcac gggaccgggt gggagcactg gtgggactgg atgccgcacg 1140

.......... .......... ORF3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 1200

1141 tcacgaagtc ggcgatgggg ctggtgtact tcgtcgtgct gaagaagttc aggcggtacg 1200

.......... .......... ORF3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 1260

1201 ggcttccgat gcaggtcctc gcgtggtaga agaagttcct gctgccgttg atgttctggg 1260

.......... .......... ORF3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 1320

1261 cgcggctcca cttcaagctc ccgctgtggg accacttggc gtagctcgac ttcccgtagc 1320

.......... .......... ORF3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 1380

1321 tgaagttcct cctgccgttg taggaccccg tgttcgacct catgttgatg ttgtcggtgt 1380

.......... .......... ORF3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 1440

1381 tgcagatata gtaccggctg ttcgtcttct tgccgtagtt ccacttgaag ttctaggcgg 1440

.......... .......... ORF3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 1500

1441 tgttgtagct cctgccgtcg cacgtcgagc ggctggtgat ggtcgtcttg tgggggtagc 1500

.......... .......... ORF3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 1560

1501 cgctgccggg gcacgacgac gggctgttgg tgatggactc gtgggtcagg cgggactcgt 1560

.......... .......... ORF3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 1620

1561 ttctggggtt gctcttcgcg ctagtgtacc aggacgacct caagcactgg cggcggccct 1620

.......... .......... ORF3 EGFP EGFP_C .......... ..........

.......... .......... ......... EcoRV(1655) ......... ..........

1621 tcactctcgg catggacgag ctgtacaagt aagatatcaa gcttatcgat aatcaacctc 1680

1621 agtgagagcc gtacctgctc gacatgttca ttctatagtt cgaatagcta ttagttggag 1680

.......... ORF3 EGFP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 1740

1681 acctaatgtt ttaaacactt tctaactgac cataagaatt gatacaacga ggaaaatgcg 1740

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt a tggctttca 1800

1741 atacacctat gcgacgaaat tacggaaaca tagtacgata acgaagggca taccgaaagt 1800

.......... .......... WPRE .......... ORF1 ..........

.......... .......... .......... .......... .......... ..........

1801 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 1860

1801 aaaagaggag gaacatattt aggaccaacg acagagaaat actcctcaac accgggcaac 1860

.......... .......... WPRE ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 1920

1861 agtccgttgc accgcaccac acgtgacaca aacgactgcg ttgggggtga ccaaccccgt 1920

.......... .......... WPRE ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 1980

1921 aacggtggtg gacagtcgag gaaaggccct gaaagcgaaa gggggaggga taacggtgcc 1980

.......... .......... WPRE ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 2040

1981 gccttgagta gcggcggacg gaacgggcga cgacctgtcc ccgagccgac aacccgtgac 2040

.......... .......... WPRE ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg 2100

2041 tgttaaggca ccacaacagc ccctttagta gcaggaaagg aaccgacgag cggacacaac 2100

.......... .......... WPRE ORF1 .......... .......... ..........

.......... ......... AatII(2124) ......... .......... ..........

2101 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 2160

2101 ggtggaccta agacgcgccc tgcaggaaga cgatgcaggg aagccgggag ttaggtcgcc 2160

.......... .......... WPRE ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 2220

2161 tggaaggaag ggcgccggac gacggccgag acgccggaga aggcgcagaa gcggaagcgg 2220

.......... .......... WPRE ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag 2280

2221 gagtctgctc agcctagagg gaaacccggc ggaggggcgt agctatggca gctggagctc 2280

.......... WPRE ORF1 .......... ......... pBluesKS_pri

.......... .......... .......... .......... .......... ..........

2281 aacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt 2340

2281 ttggatcttt ttgtacctcg ttagtgttca tcgttatgtc gtcgatggtt acgactaaca 2340

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... KpnI(2398)

2341 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct 2400

2341 cggaccgatc ttcgtgttct cctcctcctc cacccaaaag gtcagtgtgg agtccatgga 2400

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 2460

2401 aattctggtt actgaatgtt ccgtcgacat ctagaatcgg tgaaaaattt tcttttcccc 2460

.......... .......... ORF1 .......... .......... cPPT U3PPT

.......... .......... .......... .......... .......... ..........

2461 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg 2520

2461 cctgaccttc ccgattaagt gagggttgct tctgttctag acgaaaaacg aacatgaccc 2520

cPPT U3PPT .......... ORF1 delta_U3 .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 2580

2521 agagagacca atctggtcta gactcggacc ctcgagagac cgattgatcc cttgggtgac 2580

......... ORF1 HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 2640

2581 gaattcggag ttatttcgaa cggaactcac gaagttcatc acacacgggc agacaacaca 2640

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 2700

2641 ctgagaccat tgatctctag ggagtctggg aaaatcagtc acacctttta gagatcgtca 2700

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 2760

2701 tcatcaagta cagtagaata ataagtcata aatattgaac gtttctttac ttatagtctc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 2820

2761 tcactctcct tgaacaaata acgtcgaata ttaccaatgt ttatttcgtt atcgtagtgt 2820

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

2821 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 2880

2821 ttaaagtgtt tatttcgtaa aaaaagtgac gtaagatcaa caccaaacag gtttgagtag 2880

.......... ......... SV40_PA_term ......... EBV_r ..........

.......... .......... .......... .......... .......... ..........

2881 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc 2940

2881 ttacatagaa tagtacagac cgagatcgat agggcgggga ttgaggcggg tagggcgggg 2940

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 3000

2941 attgaggcgg gtcaaggcgg gtaagaggcg gggtaccgac tgattaaaaa aaataaatac 3000

.......... .......... ......... SV40_origin ......... ..........

.......... .......... .......... .......... .......... ..........

3001 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 3060

3001 gtctccggct ccggcggagc cggagactcg ataaggtctt catcactcct ccgaaaaaac 3060

SV40pro_F .......... .......... .......... .......... ..........

StuI(3064) .......... .......... .......... .......... ..........

3061 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc 3120

3061 ctccggatcc ctgcatgggt taagcgggat atcactcagc ataatgcgcg cgagtgaccg 3120

.......... .......... .......... T7_pro .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 3180

3121 gcagcaaaat gttgcagcac tgaccctttt gggaccgcaa tgggttgaat tagcggaacg 3180

M13_f20 M13_pUC_f .......... lacZ_a .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 3240

3181 tcgtgtaggg ggaaagcggt cgaccgcatt atcgcttctc cgggcgtggc tagcgggaag 3240

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc 3300

3241 ggttgtcaac gcgtcggact taccgcttac cctgcgcggg acatcgccgc gtaattcgcg 3300

.......... lacZ_a .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

3301 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 3360

3301 ccgcccacac caccaatgcg cgtcgcactg gcgatgtgaa cggtcgcggg atcgcgggcg 3360

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 3420

3361 aggaaagcga aagaagggaa ggaaagagcg gtgcaagcgg ccgaaagggg cagttcgaga 3420

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 3480

3421 tttagccccc gagggaaatc ccaaggctaa atcacgaaat gccgtggagc tggggttttt 3480

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 3540

3481 tgaactaatc ccactaccaa gtgcatcacc cggtagcggg actatctgcc aaaaagcggg 3540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 3600

3541 aaactgcaac ctcaggtgca agaaattatc acctgagaac aaggtttgac cttgttgtga 3600

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 3660

3601 gttgggatag agccagataa gaaaactaaa tattccctaa aacggctaaa gccggataac 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct 3720

3661 caatttttta ctcgactaaa ttgtttttaa attgcgctta aaattgtttt ataattgcga 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 3780

3721 atgttaaatc caccgtgaaa agccccttta cacgcgcctt ggggataaac aaataaaaag 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 3840

3781 atttatgtaa gtttatacat aggcgagtac tctgttattg ggactattta cgaagttatt 3840

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 3900

3841 ataacttttt ccttctcata ctcataagtt gtaaaggcac agcgggaata agggaaaaaa 3900

.......... .......... ....... ORF1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3901 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 3960

3901 cgccgtaaaa cggaaggaca aaaacgagtg ggtctttgcg accactttca ttttctacga 3960

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 4020

3961 cttctagtca acccacgtgc tcacccaatg tagcttgacc tagagttgtc gccattctag 4020

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 4080

4021 gaactctcaa aagcggggct tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat 4080

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 4140

4081 acaccgcgcc ataatagggc ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg 4140

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 4200

4141 ataagagtct tactgaacca actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg 4200

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 4260

4201 tactgtcatt ctcttaatac gtcacgacgg tattggtact cactattgtg acgccggttg 4260

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 4320

4261 aatgaagact gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc 4320

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 4380

4321 ctagtacatt gagcggaact agcaaccctt ggcctcgact tacttcggta tggtttgctg 4380

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 4440

4381 ctcgcactgt ggtgctacgg acatcgttac cgttgttgca acgcgtttga taattgaccg 4440

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 4500

4441 cttgatgaat gagatcgaag ggccgttgtt aattatctga cctacctccg cctatttcaa 4500

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 4560

4501 cgtcctggtg aagacgcgag ccgggaaggc cgaccgacca aataacgact atttagacct 4560

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 4620

4561 cggccactcg cacccagagc gccatagtaa cgtcgtgacc ccggtctacc attcgggagg 4620

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 4680

4621 gcatagcatc aatagatgtg ctgcccctca gtccgttgat acctacttgc tttatctgtc 4680

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 4740

4681 tagcgactct atccacggag tgactaattc gtaaccattg acagtctggt tcaaatgagt 4740

....... ORF1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 4800

4741 atatatgaaa tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 4860

4801 gaaaaactat tagagtactg gttttaggga attgcactca aaagcaaggt gactcgcagt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 4920

4861 ctggggcatc ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg 4920

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4921 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 4980

4921 acgaacgttt gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 5040

4981 ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaagaa 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 5100

5041 gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 5160

5101 cgagacgatt aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 5220

5161 aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 5280

5221 acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 5340

5281 gatactcttt cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 5400

5341 tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 5460

5401 tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta aaaacactac gagcagtccc 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 5520

5461 cccgcctcgg ataccttttt gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg 5520

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 5580

5521 accggaaaac gagtgtacaa gaaaggacgc aataggggac taagacacct attggcataa 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 5640

5581 tggcggaaac tcactcgact atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 5700

5641 cactcgctcc ttcgccttct cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 5760

5701 taagtaatta cgtcgaccgt gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 5820

5761 cgttaattac actcaatcga gtgagtaatc cgtggggtcc gaaatgtgaa atacgaaggc 5820

.......... .......... .......... .......... .......... lac_pro

.......... .......... .......... .......... .......... ..........

5821 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 5880

5821 cgagcataca acacacctta acactcgcct attgttaaag tgtgtccttt gtcgatactg 5880

lac_pro .......... .......... M13_pUC_r .......... M13_r

.......... .......... .......... .......... .......... ..........

5881 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa 5940

5881 gtactaatgc ggttcgcgcg ttaattggga gtgatttccc ttgttttcga cctcgacgtt 5940

.......... .......... T3_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc 6000

5941 cgaattacat cagaatacgt tatgagaaca tcagaacgtt gtaccattgc tactcaatcg 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt 6060

6001 ttgtacggaa tgttcctctc tttttcgtgg cacgtacggc taaccacctt cattccacca 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg 6120

6061 tgctagcacg gaataatcct tccgttgtct gcccagactg tacctaacct gcttggtgac 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc 6180

6121 ttaacggcgt aacgtctcta taacataaat tcacggatcg agctatgtat ttgcccagag 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6240

6181 agaccaatct ggtctagact cggaccctcg agagaccgat tgatcccttg ggtgacgaat 6240

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6300

6241 tcggagttat ttcgaacgga actcacgaag ttcatcacac acgggcagac aacacactga 6300

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 6360

6301 gaccattgat ctctagggag tctgggaaaa tcagtcacac cttttagaga tcgtcaccgc 6360

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg 6420

6361 gggcttgtcc ctgaactttc gctttccctt tggtctcctc gagagagctg cgtcctgagc 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat 6480

6421 cgaacgactt cgcgcgtgcc gttctccgct ccccgccgct gaccactcat gcggttttta 6480

.......... .......... .......... .......... .......... HIV-1

.......... .......... .......... .......... .......... ..........

6481 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg 6540

6481 aaactgatcg cctccgatct tcctctctct acccacgctc tcgcagtcat aattcgcccc 6540

.......... HIV-1 .......... .......... .......... ..........

.......... NruI(6553) .......... .......... .......... ..........

6541 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa 6600

6541 ctcttaatct agcgctaccc ttttttaagc caattccggt ccccctttct tttttatatt 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct 6660

6601 taattttgta tatcataccc gttcgtccct cgatcttgct aagcgtcaat taggaccgga 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac 6720

6661 caatctttgt agtcttccga catctgttta tgaccctgtc gatgttggta gggaagtctg 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca 6780

6721 tcctagtctt cttgaatcta gtaatatatt atgtcatcgt tgggagataa cacacgtagt 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa 6840

6781 ttcctatctc tattttctgt ggttccttcg aaatctgttc tatctccttc tcgttttgtt 6840

.......... .......... .......... .......... .......... ..........

.......... .......... NotI(6864) .......... .......... ..........

6841 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga 6900

6841 ttcattctgg tggcgtgtcg ttcgccggcg actagaagtc tggacctcct cctctatact 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag 6960

6901 ccctgttaac ctcttcactt aatatattta tatttcatca tttttaactt ggtaatcctc 6960

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag 7020

6961 atcgtgggtg gttccgtttc tcttctcacc acgtctctct tttttctcgt cacccttatc 7020

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga 7080

7021 ctcgaaacaa ggaacccaag aaccctcgtc gtccttcgtg atacccgcgt cgcagttact 7080

.......... .......... ORF3 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc 7140

7081 gcgactgcca tgtccggtct gttaataaca gaccatatca cgtcgtcgtc ttgttaaacg 7140

.......... .......... ORF3 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc 7200

7141 actcccgata actccgcgtt gtcgtagaca acgttgagtg tcagaccccg tagttcgtcg 7200

.......... .......... ORF3 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt 7260

7201 aggtccgttc ttaggaccga cacctttcta tggatttcct agttgtcgag gacccctaaa 7260

.......... .......... ORF3 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta 7320

7261 ccccaacgag accttttgag taaacgtggt gacgacacgg aaccttacga tcaacctcat 7320

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta 7380

7321 tatttagaga ccttgtctaa accttagtgt gctggaccta cctcaccctg tctctttaat 7380

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga 7440

7381 tgttaatgtg ttcgaattat gtgaggaatt aacttcttag cgttttggtc gttcttttct 7440

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa 7500

7441 tacttgttct taataacctt aatctattta cccgttcaaa caccttaacc aaattgtatt 7500

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa 7560

7501 gtttaaccga caccatatat tttaataagt attactatca tcctccgaac catccaaatt 7560

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat 7620

7561 cttatcaaaa acgacatgaa agatatcact tatctcaatc cgtccctata agtggtaata 7620

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag 7680

7621 gcaaagtctg ggtggagggt tggggctccc ctgggctgtc cgggcttcct tatcttcttc 7680

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc 7740

7681 ttccacctct ctctctgtct ctgtctaggt aagctaatca cttgcctaga gctgccatag 7740

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 gatcacgaga ctagcctcga ccacgacgca atggagaaag acggagagcg ccaacggcgt 7800

7741 ctagtgctct gatcggagct ggtgctgcgt tacctctttc tgcctctcgc ggttgccgca 7800

ORF3 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 ccatctcgaa ggagtcgcca gcgataaccg gagtagttga aatggtaata agacgaccaa 7860

7801 ggtagagctt cctcagcggt cgctattggc ctcatcaact ttaccattat tctgctggtt 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 tctgaccagc aaggaagcca agatgggaaa ggtcatgcgg catacgctcg gcgccagttt 7920

7861 agactggtcg ttccttcggt tctacccttt ccagtacgcc gtatgcgagc cgcggtcaaa 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 gaatattaga cataatttat cctcaagtaa gggggtacac gcgtcagtcg agtcctgtcg 7980

7921 cttataatct gtattaaata ggagttcatt cccccatgtg cgcagtcagc tcaggacagc 7980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 acaaggtcgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat 8040

7981 tgttccagcc cgtccttctc ccggataaag ggtactaagg aagtataaac gtatatgcta 8040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac 8100

8041 tgttccgaca atctctctat taaccttaat taaactgaca tttgtgtttc tataatcatg 8100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt 8160

8101 ttttatgcac tgcatctttc attattaaag aacccatcaa acgtcaaaat tttaatacaa 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 ttaaaatgga ctat...... .......... .......... .......... .......... 8174

8161 aattttacct gata...... .......... .......... .......... .......... 8174

.......... .......... .......... .......... .......... ..........