Sequence of pLOVE

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pLOVE

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pLOVE empty vector : 8263 BP; 2218 A; 1893 C; 2047 G; 2105 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 60

1 cgaattcgcc agctgcctag ccctctagag ggctagggga taccacgtga gagtcatgtt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 120

61 agacgagact acggcgtatc aattcggtca tagacgaggg acgaacacac aacctccagc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 180

121 gactcatcac gcgctcgttt taaattcgat gttgttccgt tccgaactgg ctgttaacgt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 240

181 acttcttaga cgaatcccaa tccgcaaaac gcgacgaagc gctacatgcc cggtctatat 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 300

241 gcgcaactgt aactaataac tgatcaataa ttatcattag ttaatgcccc agtaatcaag 300

.......... .......... ......... CMV_imm_pro ......... ..........

.......... .......... .......... .......... .......... ..........

301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 360

301 tatcgggtat atacctcaag gcgcaatgta ttgaatgcca tttaccgggc ggaccgactg 360

.......... ......... CMV_imm_pro ......... CAG_enh ..........

.......... .......... .......... .......... .......... ..........

361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 420

361 gcgggttgct gggggcgggt aactgcagtt attactgcat acaagggtat cattgcggtt 420

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 480

421 atccctgaaa ggtaactgca gttacccacc tcataaatgc catttgacgg gtgaaccgtc 480

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 540

481 atgtagttca catagtatac ggttcatgcg ggggataact gcagttactg ccatttaccg 540

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 600

541 ggcggaccgt aatacgggtc atgtactgga ataccctgaa aggatgaacc gtcatgtaga 600

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 660

601 tgcataatca gtagcgataa tggtaccact acgccaaaac cgtcatgtag ttacccgcac 660

CAG_enh ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 720

661 ctatcgccaa actgagtgcc cctaaaggtt cagaggtggg gtaactgcag ttaccctcaa 720

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 780

721 acaaaaccgt ggttttagtt gccctgaaag gttttacagc attgttgagg cggggtaact 780

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 840

781 gcgtttaccc gccatccgca catgccaccc tccagatata ttcgtcgcgc aaaacggaca 840

......... CMV_f_pri CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 900

841 tgacccagag agaccaatct ggtctagact cggaccctcg agagaccgat tgatcccttg 900

.......... .......... ......... HIV truncHIV ......... ..........

.......... .......... .......... .......... .......... ..........

901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 960

901 ggtgacgaat tcggagttat ttcgaacgga actcacgaag ttcatcacac acgggcagac 960

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 1020

961 aacacactga gaccattgat ctctagggag tctgggaaaa tcagtcacac cttttagaga 1020

.......... ......... HIV truncHIV ......... .......... ..........

NarI(1028) .......... .......... .......... .......... ..........

1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 1080

1021 tcgtcaccgc gggcttgtcc ctgaactttc gctttccctt tggtctcctc gagagagctg 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 1140

1081 cgtcctgagc cgaacgactt cgcgcgtgcc gttctccgct ccccgccgct gaccactcat 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 1200

1141 gcggttttta aaactgatcg cctccgatct tcctctctct acccacgctc tcgcagtcat 1200

.......... HIV-1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1260

1201 aattcgcccc ctcttaatct agcgctaccc ttttttaagc caattccggt ccccctttct 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1320

1261 tttttatatt taattttgta tatcataccc gttcgtccct cgatcttgct aagcgtcaat 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1380

1321 taggaccgga caatctttgt agtcttccga catctgttta tgaccctgtc gatgttggta 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1440

1381 gggaagtctg tcctagtctt cttgaatcta gtaatatatt atgtcatcgt tgggagataa 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1500

1441 cacacgtagt ttcctatctc tattttctgt ggttccttcg aaatctgttc tatctccttc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 1560

1501 tcgttttgtt ttcattctgg tggcgtgtcg ttcgccggcc ggcgcgacta gaagtctgga 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 1620

1561 cctcctcctc tatactccct gttaacctct tcacttaata tatttatatt tcatcatttt 1620

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 1680

1621 taacttggta atcctcatcg tgggtggttc cgtttctctt ctcaccacgt ctctcttttt 1680

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 1740

1681 tctcgtcacc cttatcctcg aaacaaggaa cccaagaacc ctcgtcgtcc ttcgtgatac 1740

.......... .......... ORF 1 RRE .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 1800

1741 ccgcgtcgca gttactgcga ctgccatgtc cggtctgtta ataacagacc atatcacgtc 1800

.......... .......... ORF 1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 1860

1801 gtcgtcttgt taaacgactc ccgataactc cgcgttgtcg tagacaacgt tgagtgtcag 1860

.......... .......... ORF 1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 1920

1861 accccgtagt tcgtcgaggt ccgttcttag gaccgacacc tttctatgga tttcctagtt 1920

.......... .......... ORF 1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 1980

1921 gtcgaggacc cctaaacccc aacgagacct tttgagtaaa cgtggtgacg acacggaacc 1980

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 2040

1981 ttacgatcaa cctcattatt tagagacctt gtctaaacct tagtgtgctg gacctacctc 2040

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 2100

2041 accctgtctc tttaattgtt aatgtgttcg aattatgtga ggaattaact tcttagcgtt 2100

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 2160

2101 ttggtcgttc ttttcttact tgttcttaat aaccttaatc tatttacccg ttcaaacacc 2160

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 2220

2161 ttaaccaaat tgtattgttt aaccgacacc atatatttta ataagtatta ctatcatcct 2220

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 2280

2221 ccgaaccatc caaattctta tcaaaaacga catgaaagat atcacttatc tcaatccgtc 2280

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 2340

2281 cctataagtg gtaatagcaa agtctgggtg gagggttggg gctcccctgg gctgtccggg 2340

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 2400

2341 cttccttatc ttcttcttcc acctctctct ctgtctctgt ctaggtaagc taatcacttg 2400

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 2460

2401 cctagccgtg acgcacgcgg ttaagacgtc tgtttaccgt cataagtagg tgttaaaatt 2460

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2520

2461 ttcttttccc ccctaacccc ccatgtcacg tcccctttct tatcatctgt attatcgttg 2520

cPPT .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 2580

2521 tctgtatgtt tgatttctta atgtttttgt ttaatgtttt taagttttaa aagcccaaat 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctagc agttccgcgt 2640

2581 aatgtccctg tcgtctctag gtcaaaccaa tcatggcccg ggcgagatcg tcaaggcgca 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac 2700

2641 atgtattgaa tgccatttac cgggcggacc gactggcggg ttgctggggg cgggtaactg 2700

.......... ......... CMV_imm_pro CAG_enh ......... ..........

.......... .......... .......... .......... .......... ..........

2701 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg 2760

2701 cagttattac tgcatacaag ggtatcattg cggttatccc tgaaaggtaa ctgcagttac 2760

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag 2820

2761 ccacctcata aatgccattt gacgggtgaa ccgtcatgta gttcacatag tatacggttc 2820

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat 2880

2821 atgcggggga taactgcagt tactgccatt taccgggcgg accgtaatac gggtcatgta 2880

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat 2940

2881 ctggaatacc ctgaaaggat gaaccgtcat gtagatgcat aatcagtagc gataatggta 2940

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt 3000

2941 ccactacgcc aaaaccgtca tgtagttacc cgcacctatc gccaaactga gtgcccctaa 3000

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga 3060

3001 aggttcagag gtggggtaac tgcagttacc ctcaaacaaa accgtggttt tagttgccct 3060

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta g gcgtgtacg 3120

3061 gaaaggtttt acagcattgt tgaggcgggg taactgcgtt tacccgccat ccgcacatgc 3120

.......... ......... CMV_imm_pro ......... CMV_f_pri ..........

.......... .......... .......... .......... .......... ..........

3121 gtgggaggtc tatataagca gagctctccc tatcagtgat agagatctcc ctatcagtga 3180

3121 caccctccag atatattcgt ctcgagaggg atagtcacta tctctagagg gatagtcact 3180

CMV_imm_pro ........ .......... .......... tetO ..........

.......... .......... .......... .......... .......... ..........

3181 tagagatcgt cgactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa 3240

3181 atctctagca gctgatcagg tcacaccacc ttaagacgtc tatagttgtt caaacatgtt 3240

.......... .......... .......... .......... .......... attR1

.......... .......... .......... .......... .......... ..........

3241 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca 3300

3241 ttttcgactt gctctttgca ttttactata tttatagtta tataatttaa tctaaaacgt 3300

.......... .......... attR1 attR2 .......... ..........

.......... .......... .......... .......... .......... ..........

3301 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca 3360

3301 attttttgtc tgatgtatta tgacattttg tgttgtatag gtcagtgata ccgccggcgt 3360

.......... ......... attR1 attR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt 3420

3361 aatccgtggg gtccgaaatg tgaaatacga aggccgagca tattacacac ctaaaactca 3420

.......... .......... lac .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 taggatccgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga 3480

3421 atcctaggcc gctctaaaag tcctcgattc cttcgatttt acctcttttt ttagtgacct 3480

.......... .......... .......... ........ ORF 1 CAT/CamR ........

.......... .......... .......... .......... .......... ..........

3481 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca 3540

3481 atatggtggc aactatatag ggttaccgta gcatttcttg taaaactccg taaagtcagt 3540

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc 3600

3541 caacgagtta catggatatt ggtctggcaa gtcgacctat aatgccggaa aaatttctgg 3600

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg 3660

3601 catttctttt tattcgtgtt caaaataggc cggaaataag tgtaagaacg ggcggactac 3660

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt 3720

3661 ttacgagtag gccttaaggc ataccgttac tttctgccac tcgaccacta taccctatca 3720

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt 3780

3721 caagtgggaa caatgtggca aaaggtactc gtttgacttt gcaaaagtag cgagacctca 3780

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac 3840

3781 cttatggtgc tgctaaaggc cgtcaaagat gtgtatataa gcgttctaca ccgcacaatg 3840

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc 3900

3841 ccacttttgg accggataaa gggatttccc aaataactct tatacaaaaa gcagagtcgg 3900

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc 3960

3901 ttagggaccc actcaaagtg gtcaaaacta aatttgcacc ggttatacct gttgaagaag 3960

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg 4020

3961 cgggggcaaa agtggtaccc gtttataata tgcgttccgc tgttccacga ctacggcgac 4020

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa 4080

4021 cgctaagtcc aagtagtacg gcagacacta ccgaaggtac agccgtctta cgaattactt 4080

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc cggcttacta 4140

4081 aatgttgtca tgacgctact caccgtcccg ccccgcattt ctagacctag gccgaatgat 4140

........ ORF 1 CAT/CamR ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact 4200

4141 tttcggtcta ttgtcatacg cataaacgcg cgactaaaaa cgccatattc ttatatatga 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga 4260

4201 ctatacatat gggcttcata cagtttttct ccacacgata cttcgtcgca taatgtcact 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct 4320

4261 gtcaactgtc gctgtcgata gtcaacgagt tccgtatata ctacagttat agaggccaga 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga 4380

4321 ccattcgtgt tggtacgtct tacttcgggc agcagacgca cggcttgcga cctttcgcct 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga 4440

4381 tttagtcctt ccctaccgac tccagcgggc caaataactt tacttgccga gaaaacgact 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt 4500

4441 gctcttgtcc ctgaccactt tacgtcaaat tccaaatgtg gatattttct ctctcggcaa 4500

.......... .......... .......... ccdB .......... ..........

.......... .......... .......... .......... SmaI(4544) ..........

4501 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga 4560

4501 tagcagacaa acacctacat gtctcactat aataactgtg cgggcccgct gcctaccact 4560

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg 4620

4561 agggggaccg gtcacgtgca gacgacagtc tatttcagag ggcacttgaa atgggccacc 4620

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct 4680

4621 acgtatagcc cctactttcg accgcgtact actggtggct ataccggtca cacggccaga 4680

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca 4740

4681 ggcaatagcc ccttcttcac cgactagagt cggtggcgct tttactgtag tttttgcggt 4740

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ttaacctgat gttctgggga atataaatgt caggctccgt tatacacagc cagtctgcag 4800

4741 aattggacta caagacccct tatatttaca gtccgaggca atatgtgtcg gtcagacgtc 4800

.......... ccdB .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc 4860

4801 cagctggtat cactgaccta tacaacacaa aatgtcataa tacatcagac aaaaaatacg 4860

.......... .......... .......... attR1 .......... attR2

.......... .......... .......... .......... .......... ..........

4861 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg 4920

4861 ttttagatta aattatataa ctataaatat agtaaaatgc aaagagcaag tcgaaagaac 4920

.......... ......... attR1 attR2 ......... .......... ..........

.......... .......... .......... XhoI(4956) XbaI(4962) ..........

4921 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc 4980

4921 atgtttcacc aactataggt cgtgtcaccg ccggcgagct cagatctccc gggcgccaag 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... AgeI(5029) ..........

4981 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa 5040

4981 cttccattcg gatagggatt gggagaggag ccagagctaa gatgcgcatg gccaatcatt 5040

.......... .......... V5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tgacaattcg tcgagggacc taataacttc gtatagcata cattatacga agttatacat 5100

5041 actgttaagc agctccctgg attattgaag catatcgtat gtaatatgct tcaatatgta 5100

.......... .......... .......... loxP .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct 5160

5101 caaattccca aggccaaggt gatccatgtt aagctatagt tcgaatagct attagttgga 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 5220

5161 gacctaatgt tttaaacact ttctaactga ccataagaat tgatacaacg aggaaaatgc 5220

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg t atggctttc 5280

5221 gatacaccta tgcgacgaaa ttacggaaac atagtacgat aacgaagggc ataccgaaag 5280

.......... .......... WPRE .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

5281 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 5340

5281 taaaagagga ggaacatatt taggaccaac gacagagaaa tactcctcaa caccgggcaa 5340

.......... .......... WPRE ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 5400

5341 cagtccgttg caccgcacca cacgtgacac aaacgactgc gttgggggtg accaaccccg 5400

.......... .......... WPRE ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 5460

5401 taacggtggt ggacagtcga ggaaaggccc tgaaagcgaa agggggaggg ataacggtgc 5460

.......... .......... WPRE ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 5520

5461 cgccttgagt agcggcggac ggaacgggcg acgacctgtc cccgagccga caacccgtga 5520

.......... .......... WPRE ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt 5580

5521 ctgttaaggc accacaacag cccctttagt agcaggaaag gaaccgacga gcggacacaa 5580

.......... .......... WPRE ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 5640

5581 cggtggacct aagacgcgcc ctgcaggaag acgatgcagg gaagccggga gttaggtcgc 5640

.......... .......... WPRE ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 5700

5641 ctggaaggaa gggcgccgga cgacggccga gacgccggag aaggcgcaga agcggaagcg 5700

.......... .......... WPRE ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga 5760

5701 ggagtctgct cagcctagag ggaaacccgg cggaggggcg tagctatggc agctggagct 5760

.......... .......... WPRE ORF 2 .......... ......... pBluesKS_pri

.......... .......... .......... .......... .......... ..........

5761 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga 5820

5761 agctctggat ctttttgtac ctcgttagtg ttcatcgtta tgtcgtcgat ggttacgact 5820

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt 5880

5821 aacacggacc gatcttcgtg ttctcctcct cctccaccca aaaggtcagt gtggagtcca 5880

.......... .......... ORF 2 .......... .......... ..........

KpnI(5882) .......... .......... .......... .......... ..........

5881 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 5940

5881 tggaaattct ggttactgaa tgttccgtcg acatctagaa tcggtgaaaa attttctttt 5940

.......... .......... ORF 2 .......... .......... cPPT U3PPT

.......... .......... .......... .......... .......... ..........

5941 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat 6000

5941 cccccctgac cttcccgatt aagtgagggt tgcttctgtt ctataggaac tagacaccta 6000

U3PPT .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag 6060

6001 gatggtgtgt gttccgatga agggactaac cgtcttgatg tgtggtcccg gtccctagtc 6060

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga 6120

6061 tataggtgac tggaaaccta ccacgatgtt cgatcatggt caactcgttc tcttccatct 6120

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga 6180

6121 tcttcggtta cttcctctct tgtgggcgaa caatgtggga cactcggacg taccctacct 6180

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat 6240

6181 actgggcctc tctcttcata atctcacctc caaactgtcg gcggatcgta aagtagtgta 6240

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttaga ccaga tctgagcctg 6300

6241 ccgggctctc gacgtaggcc tgacatgacc cagagagacc aatctggtct agactcggac 6300

.......... .......... ORF 2 ......... HIV truncHIV .........

.......... .......... .......... .......... .......... ..........

6301 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6360

6301 cctcgagaga ccgattgatc ccttgggtga cgaattcgga gttatttcga acggaactca 6360

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6420

6361 cgaagttcat cacacacggg cagacaacac actgagacca ttgatctcta gggagtctgg 6420

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag 6480

6421 gaaaatcagt cacacctttt agagatcgtc gtacactcgt tttccggtcg ttttccggtc 6480

......... HIV truncHIV ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 6540

6481 cttggcattt ttccggcgca acgaccgcaa aaaggtatcc gaggcggggg gactgctcgt 6540

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

6541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 6600

6541 agtgttttta gctgcgagtt cagtctccac cgctttgggc tgtcctgata tttctatggt 6600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 6660

6601 ccgcaaaggg ggaccttcga gggagcacgc gagaggacaa ggctgggacg gcgaatggcc 6660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 6720

6661 tatggacagg cggaaagagg gaagcccttc gcaccgcgaa agagtatcga gtgcgacatc 6720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 6780

6721 catagagtca agccacatcc agcaagcgag gttcgacccg acacacgtgc ttggggggca 6780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 6840

6781 agtcgggctg gcgacgcgga ataggccatt gatagcagaa ctcaggttgg gccattctgt 6840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 6900

6841 gctgaatagc ggtgaccgtc gtcggtgacc attgtcctaa tcgtctcgct ccatacatcc 6900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6901 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 6960

6901 gccacgatgt ctcaagaact tcaccaccgg attgatgccg atgtgatctt cttgtcataa 6960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7020

6961 accatagacg cgagacgact tcggtcaatg gaagcctttt tctcaaccat cgagaactag 7020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7021 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7080

7021 gccgtttgtt tggtggcgac catcgccacc aaaaaaacaa acgttcgtcg tctaatgcgc 7080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7140

7081 gtcttttttt cctagagttc ttctaggaaa ctagaaaaga tgccccagac tgcgagtcac 7140

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 7200

7141 cttgcttttg agtgcaattc cctaaaacca gtactctaat agtttttcct agaagtggat 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 7260

7201 ctaggaaaat ttaattttta cttcaaaatt tagttagatt tcatatatac tcatttgaac 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 7320

7261 cagactgtca atggttacga attagtcact ccgtggatag agtcgctaga cagataaagc 7320

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

7321 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 7380

7321 aagtaggtat caacggactg aggggcagca catctattga tgctatgccc tcccgaatgg 7380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 7440

7381 tagaccgggg tcacgacgtt actatggcgc tctgggtgcg agtggccgag gtctaaatag 7440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 7500

7441 tcgttatttg gtcggtcggc cttcccggct cgcgtcttca ccaggacgtt gaaataggcg 7500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 7560

7501 gaggtaggtc agataattaa caacggccct tcgatctcat tcatcaagcg gtcaattatc 7560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

FspI(7565) .......... .......... .......... .......... ..........

7561 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 7620

7561 aaacgcgttg caacaacggt aacgatgtcc gtagcaccac agtgcgagca gcaaaccata 7620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 7680

7621 ccgaagtaag tcgaggccaa gggttgctag ttccgctcaa tgtactaggg ggtacaacac 7680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 7740

7681 gttttttcgc caatcgagga agccaggagg ctagcaacag tcttcattca accggcgtca 7740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 7800

7741 caatagtgag taccaatacc gtcgtgacgt attaagagaa tgacagtacg gtaggcattc 7800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 7860

7801 tacgaaaaga cactgaccac tcatgagttg gttcagtaag actcttatca catacgccgc 7860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 7920

7861 tggctcaacg agaacgggcc gcagttatgc cctattatgg cgcggtgtat cgtcttgaaa 7920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 7980

7921 ttttcacgag tagtaacctt ttgcaagaag ccccgctttt gagagttcct agaatggcga 7980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 8040

7981 caactctagg tcaagctaca ttgggtgagc acgtgggttg actagaagtc gtagaaaatg 8040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 8100

8041 aaagtggtcg caaagaccca ctcgtttttg tccttccgtt ttacggcgtt ttttccctta 8100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 8160

8101 ttcccgctgt gcctttacaa cttatgagta tgagaaggaa aaagttataa taacttcgta 8160

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 8220

8161 aatagtccca ataacagagt actcgcctat gtataaactt acataaatct ttttatttgt 8220

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac....... .......... 8263

8221 ttatccccaa ggcgcgtgta aaggggcttt tcacggtgga ctg....... .......... 8263

.......... .......... .......... .......... .......... ..........