Sequence of pLP-HA

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Sequence of pLP-HA

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Sequence of V143 pLP-HA SD - Acceptor : 4519 BP; 1099 A; 1153 C; 1176 G; 1091 T; 0 other;

AseI(7) .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... ......... CMV_imm_pro ......... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ......... CMV_imm_pro CAG_enh ......... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

.......... ......... CMV_imm_pro ..... CMV_f_pri CMV_pro ......

.......... .......... .......... .......... .......... NheI(591)

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcggtgg 600

......... CMV_imm_pro CMV_pro ......... .......... ..........

.......... .......... .......... BglII(640) .......... ..........

601 atgtacccat acgacgtgcc agactacgcc caggtaagta gatcttgacc tctcaaaagc 660

601 tacatgggta tgctgcacgg tctgatgcgg gtccattcat ctagaactgg agagttttcg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gggcatgact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac 720

661 cccgtactga agacgcgatt ctaacagtca aaggtttttg ctcctcctaa actataagtg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctggcccgcg gtgatgcctt tgagggtggc cgcatccatc tggtcagaaa agacaatctt 780

721 gaccgggcgc cactacggaa actcccaccg gcgtaggtag accagtcttt tctgttagaa 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tttgttgtca agcttggtgg caaacgaccc gtagagggcg ttggacagca acttggcgat 840

781 aaacaacagt tcgaaccacc gtttgctggg catctcccgc aacctgtcgt tgaaccgcta 840

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(865) .......... .......... ..........

841 ggagcgcagg gtttggtttt tgtcgcgatc ggcgcgctcc ttggccgcga tgtttagctg 900

841 cctcgcgtcc caaaccaaaa acagcgctag ccgcgcgagg aaccggcgct acaaatcgac 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cacgtattcg cgcgcaacgc accgccattc gggaaagacg gtggtgcgct cgtcgggcac 960

901 gtgcataagc gcgcgttgcg tggcggtaag ccctttctgc caccacgcga gcagcccgtg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 caggtgcacg cgccaaccgc ggttgtgcag ggtgacaagg tcaacgctaa gcttcgataa 1020

961 gtccacgtgc gcggttggcg ccaacacgtc ccactgttcc agttgcgatt cgaagctatt 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cttcgtatag catacattat acgaagttat agatccaata ttattgaagc atttatcagg 1080

1021 gaagcatatc gtatgtaata tgcttcaata tctaggttat aataacttcg taaatagtcc 1080

.......... loxP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 1140

1081 caataacaga gtactcgcct atgtataaac ttacataaat ctttttattt gtttatcccc 1140

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... ......... BamHI(1178) XbaI(1190) BclI(1200)

1141 ttccgcgcac atttccccga aaagtgccac ctgacgtgga tccaccggat ctagataact 1200

1141 aaggcgcgtg taaaggggct tttcacggtg gactgcacct aggtggccta gatctattga 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 1260

1201 ctagtattag tcggtatggt gtaaacatct ccaaaatgaa cgaaattttt tggagggtgt 1260

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... HpaI(1306) ..........

1261 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 1320

1261 ggagggggac ttggactttg tattttactt acgttaacaa caacaattga acaaataacg 1320

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 1380

1321 tcgaatatta ccaatgttta tttcgttatc gtagtgttta aagtgtttat ttcgtaaaaa 1380

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... ......... AflII(1425) .........

1381 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg 1440

1381 aagtgacgta agatcaacac caaacaggtt tgagtagtta catagaattc cgcatttaac 1440

.......... ...... SV40_PA_term EBV_r ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 1500

1441 attcgcaatt ataaaacaat tttaagcgca atttaaaaac aatttagtcg agtaaaaaat 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 1560

1501 tggttatccg gctttagccg ttttagggaa tatttagttt tcttatctgg ctctatccca 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 1620

1561 actcacaaca aggtcaaacc ttgttctcag gtgataattt cttgcacctg aggttgcagt 1620

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 1680

1621 ttcccgcttt ttggcagata gtcccgctac cgggtgatgc acttggtagt gggattagtt 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 1740

1681 caaaaaaccc cagctccacg gcatttcgtg atttagcctt gggatttccc tcgggggcta 1740

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 1800

1741 aatctcgaac tgcccctttc ggccgcttgc accgctcttt ccttcccttc tttcgctttc 1800

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 1860

1801 ctcgcccgcg atcccgcgac cgttcacatc gccagtgcga cgcgcattgg tggtgtgggc 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg 1920

1861 ggcgcgaatt acgcggcgat gtcccgcgca gtccaccgtg aaaagcccct ttacacgcgc 1920

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 1980

1921 cttggggata aacaaataaa aagatttatg taagtttata cataggcgag tactctgtta 1980

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

1981 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca 2040

1981 ttgggactat ttacgaagtt attataactt tttccttctc aggactccgc ctttcttggt 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 2100

2041 cgacacctta cacacagtca atcccacacc tttcaggggt ccgaggggtc gtccgtcttc 2100

......... pBABE_3_pri ......... SV40_enh SV40_pro ..........

.......... .......... .......... .......... .......... ..........

2101 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 2160

2101 atacgtttcg tacgtagagt taatcagtcg ttggtccaca cctttcaggg gtccgagggg 2160

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 2220

2161 tcgtccgtct tcatacgttt cgtacgtaga gttaatcagt cgttggtatc agggcgggga 2220

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 2280

2221 ttgaggcggg tagggcgggg attgaggcgg gtcaaggcgg gtaagaggcg gggtaccgac 2280

.......... SV40_enh SV40_pro SV40_ori .......... ..........

.......... .......... .......... .......... .......... ..........

2281 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 2340

2281 tgattaaaaa aaataaatac gtctccggct ccggcggagc cggagactcg ataaggtctt 2340

.... SV40_ori SV40_pro SV40pro_F_pri .... .......... ..........

.......... .......... StuI(2364) .......... ClaI(2383) ..........

2341 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat 2400

2341 catcactcct ccgaaaaaac ctccggatcc gaaaacgttt ctagctagtt ctctgtccta 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... EagI(2449) ..........

2401 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 2460

2401 ctcctagcaa agcgtactaa cttgttctac ctaacgtgcg tccaagaggc cggcgaaccc 2460

.......... .......... ........ ORF2 Neomycin ........ ..........

.......... .......... .......... .......... .......... ..........

2461 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 2520

2461 acctctccga taagccgata ctgacccgtg ttgtctgtta gccgacgaga ctacggcggc 2520

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... NarI(2543) .......... .......... ..........

2521 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 2580

2521 acaaggccga cagtcgcgtc cccgcgggcc aagaaaaaca gttctggctg gacaggccac 2580

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... MscI(2625) ..........

2581 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 2640

2581 gggacttact tgacgttctg ctccgtcgcg ccgatagcac cgaccggtgc tgcccgcaag 2640

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 2700

2641 gaacgcgtcg acacgagctg caacagtgac ttcgcccttc cctgaccgac gataacccgc 2700

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 2760

2701 ttcacggccc cgtcctagag gacagtagag tggaacgagg acggctcttt cataggtagt 2760

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 2820

2761 accgactacg ttacgccgcc gacgtatgcg aactaggccg atggacgggt aagctggtgg 2820

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 2880

2821 ttcgctttgt agcgtagctc gctcgtgcat gagcctacct tcggccagaa cagctagtcc 2880

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 2940

2881 tactagacct gcttctcgta gtccccgagc gcggtcggct tgacaagcgg tccgagttcc 2940

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 3000

2941 gctcgtacgg gctgccgctc ctagagcagc actgggtacc gctacggacg aacggcttat 3000

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 3060

3001 agtaccacct tttaccggcg aaaagaccta agtagctgac accggccgac ccacaccgcc 3060

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 3120

3061 tggcgatagt cctgtatcgc aaccgatggg cactataacg acttctcgaa ccgccgctta 3120

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 3180

3121 cccgactggc gaaggagcac gaaatgccat agcggcgagg gctaagcgtc gcgtagcgga 3180

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... ......... BstBI(3225) .........

3181 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 3240

3181 agatagcgga agaactgctc aagaagactc gccctgagac cccaagcttt actggctggt 3240

........ ORF2 Neomycin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 3300

3241 tcgctgcggg ttggacggta gtgctctaaa gctaaggtgg cggcggaaga tactttccaa 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 3360

3301 cccgaagcct tagcaaaagg ccctgcggcc gacctactag gaggtcgcgc ccctagagta 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc 3420

3361 cgacctcaag aagcgggtgg gatccccctc cgattgactt tgtgccttcc tctgttatgg 3420

.......... .......... .......... .......... TK_PA_term ..........

.......... .......... .......... .......... .......... ..........

3421 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc 3480

3421 ccttccttgg gcgcgatact gccgttattt ttctgtctta ttttgcgtgc cacaacccag 3480

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag 3540

3481 caaacaagta tttgcgcccc aagccagggt cccgaccgtg agacagctat ggggtggctc 3540

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc 3600

3541 tggggtaacc ccggttatgc gggcgcaaag aaggaaaagg ggtggggtgg ggggttcaag 3600

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg 3660

3601 cccacttccg ggtcccgagc gtcggttgca gccccgccgt ccgggacggt atcggagtcc 3660

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 3720

3661 aatgagtata tatgaaatct aactaaattt tgaagtaaaa attaaatttt cctagatcca 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 3780

3721 cttctaggaa aaactattag agtactggtt ttagggaatt gcactcaaaa gcaaggtgac 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 3840

3781 tcgcagtctg gggcatcttt tctagtttcc tagaagaact ctaggaaaaa aagacgcgca 3840

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3841 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 3900

3841 ttagacgacg aacgtttgtt tttttggtgg cgatggtcgc caccaaacaa acggcctagt 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 3960

3901 tctcgatggt tgagaaaaag gcttccattg accgaagtcg tctcgcgtct atggtttatg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 4020

3961 acaggaagat cacatcggca tcaatccggt ggtgaagttc ttgagacatc gtggcggatg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 4080

4021 tatggagcga gacgattagg acaatggtca ccgacgacgg tcaccgctat tcagcacaga 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 4140

4081 atggcccaac ctgagttctg ctatcaatgg cctattccgc gtcgccagcc cgacttgccc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 4200

4141 cccaagcacg tgtgtcgggt cgaacctcgc ttgctggatg tggcttgact ctatggatgt 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 4260

4201 cgcactcgat actctttcgc ggtgcgaagg gcttccctct ttccgcctgt ccataggcca 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 4320

4261 ttcgccgtcc cagccttgtc ctctcgcgtg ctccctcgaa ggtccccctt tgcggaccat 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 4380

4321 agaaatatca ggacagccca aagcggtgga gactgaactc gcagctaaaa acactacgag 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 4440

4381 cagtcccccc gcctcggata cctttttgcg gtcgttgcgc cggaaaaatg ccaaggaccg 4440

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 4500

4441 gaaaacgacc ggaaaacgag tgtacaagaa aggacgcaat aggggactaa gacacctatt 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ccgtattacc gccatgcat. .......... .......... .......... .......... 4519

4501 ggcataatgg cggtacgta. .......... .......... .......... .......... 4519

.......... .......... .......... .......... .......... ..........