Sequence of pLVCT-tTR-KRAB

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Sequence of pLVCT-tTR-KRAB

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Sequence of pLVCT-tTR-KRAB : 12877 BP; 3211 A; 3156 C; 3422 G; 3088 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta 60

1 catcaacctt cccgattaag tgagggtttc ttctgttcta taggaactag acacctagat 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata 120

61 ggtgtgtgtt ccgatgaagg gactaatcgt cttgatgtgt ggtcccggtc cccagtctat 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga 180

121 aggtgactgg aaacctacca cgatgttcga tcatggtcaa ctcggtctat tccatcttct 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga 240

181 ccggttattt cctctcttgt ggtcgaacaa tgtgggacac tcggacgtac cctacctact 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc 300

241 gggcctctct cttcacaatc tcacctccaa actgtcggcg gatcgtaaag tagtgcaccg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact 360

301 ggctctcgac gtaggcctca tgaagttctt gacgactata gctcgaacga tgttccctga 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct 420

361 aaggcgaccc ctgaaaggtc cctccgcacc ggacccgccc tgacccctca ccgctcggga 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga 480

421 gtctaggacg tatattcgtc gacgaaaaac ggacatgacc cagagagacc aatctggtct 480

.......... .......... .......... ......... HIV truncHIV .........

.......... .......... .......... .......... .......... ..........

481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 540

481 agactcggac cctcgagaga ccgattgatc ccttgggtga cgaattcgga gttatttcga 540

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 600

541 acggaactca cgaagttcat cacacacggg cagacaacac actgagacca ttgatctcta 600

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt 660

601 gggagtctgg gaaaatcagt cacacctttt agagatcgtc accgcgggct tgtccctgaa 660

......... HIV truncHIV ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg 720

661 ctttcgcttt ccctttggtc tcctcgagag agctgcgtcc tgagccgaac gacttcgcgc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg 780

721 gtgccgttct ccgctccccg ccgctgacca ctcatgcggt ttttaaaact gatcgcctcc 780

.......... .......... .......... .......... HIV-1 ..........

.......... .......... .......... .......... .......... ..........

781 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg 840

781 gatcttcctc tctctaccca cgctctcgca gtcataattc gccccctctt aatctagcgc 840

HIV-1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt 900

841 tacccttttt taagccaatt ccggtccccc tttctttttt atatttaatt ttgtatatca 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga 960

901 tacccgttcg tccctcgatc ttgctaagcg tcaattagga ccggacaatc tttgtagtct 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact 1020

961 tccgacatct gtttatgacc ctgtcgatgt tggtagggaa gtctgtccta gtcttcttga 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa 1080

1021 atctagtaat atattatgtc atcgttggga gataacacac gtagtttcct atctctattt 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc 1140

1081 tctgtggttc cttcgaaatc tgttctatct ccttctcgtt ttgttttcat tctggtggcg 1140

.......... .......... .......... .......... .......... ..........

NotI(1149) .......... .......... .......... .......... ..........

1141 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa 1200

1141 tgtcgttcgc cggcgactag aagtctggac ctcctcctct atactccctg ttaacctctt 1200

.......... .......... .......... .......... .......... ORF 3

.......... .......... .......... .......... .......... ..........

1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg 1260

1201 cacttaatat atttatattt catcattttt aacttggtaa tcctcatcgt gggtggttcc 1260

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg 1320

1261 gtttctcttc tcaccacgtc tctctttttt ctcgtcaccc ttatcctcga aacaaggaac 1320

.......... .......... ORF 3 .......... .......... RRE

.......... .......... .......... .......... .......... ..........

1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg 1380

1321 ccaagaaccc tcgtcgtcct tcgtgatacc cgcgtcgcag ttactgcgac tgccatgtcc 1380

.......... .......... ORF 3 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg 1440

1381 ggtctgttaa taacagacca tatcacgtcg tcgtcttgtt aaacgactcc cgataactcc 1440

.......... .......... ORF 3 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc 1500

1441 gcgttgtcgt agacaacgtt gagtgtcaga ccccgtagtt cgtcgaggtc cgttcttagg 1500

.......... .......... ORF 3 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa 1560

1501 accgacacct ttctatggat ttcctagttg tcgaggaccc ctaaacccca acgagacctt 1560

.......... RRE ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac 1620

1561 ttgagtaaac gtggtgacga cacggaacct tacgatcaac ctcattattt agagaccttg 1620

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct 1680

1621 tctaaacctt agtgtgctgg acctacctca ccctgtctct ttaattgtta atgtgttcga 1680

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat 1740

1681 attatgtgag gaattaactt cttagcgttt tggtcgttct tttcttactt gttcttaata 1740

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt 1800

1741 accttaatct atttacccgt tcaaacacct taaccaaatt gtattgttta accgacacca 1800

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg 1860

1801 tatattttaa taagtattac tatcatcctc cgaaccatcc aaattcttat caaaaacgac 1860

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc 1920

1861 atgaaagata tcacttatct caatccgtcc ctataagtgg taatagcaaa gtctgggtgg 1920

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag 1980

1921 agggttgggg ctcccctggg ctgtccgggc ttccttatct tcttcttcca cctctctctc 1980

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc 2040

1981 tgtctctgtc taggtaagct aatcacttgc ctagagctgc catagctagt gctctgatcg 2040

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctcgaccatc gatgtcgatc gacattgatt attgactagt tattaatagt aatcaattac 2100

2041 gagctggtag ctacagctag ctgtaactaa taactgatca ataattatca ttagttaatg 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 2160

2101 ccccagtaat caagtatcgg gtatatacct caaggcgcaa tgtattgaat gccatttacc 2160

.......... .......... .......... .......... CAG_enh ..........

.......... .......... .......... .......... .......... ..........

2161 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 2220

2161 gggcggaccg actggcgggt tgctgggggc gggtaactgc agttattact gcatacaagg 2220

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 2280

2221 gtatcattgc ggttatccct gaaaggtaac tgcagttacc cacctcataa atgccatttg 2280

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 2340

2281 acgggtgaac cgtcatgtag ttcacatagt atacggttca tgcgggggat aactgcagtt 2340

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 2400

2341 actgccattt accgggcgga ccgtaatacg ggtcatgtac tggaataccc tgaaaggatg 2400

.......... .......... CAG_enh .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt 2460

2401 aaccgtcatg tagatgcata atcagtagcg ataatggtac cagctccact cggggtgcaa 2460

.......... CAG_enh .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt 2520

2461 gacgaagtga gaggggtaga ggggggggag gggtgggggt taaaacataa ataaataaaa 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttaattattt tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg 2580

2521 aattaataaa acacgtcgct acccccgccc cccccccccc cgcgcgcggt ccgccccgcc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg 2640

2581 ccgccccgct ccccgccccg ccccgctccg cctctccacg ccgccgtcgg ttagtctcgc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc 2700

2641 cgcgcgaggc tttcaaagga aaataccgct ccgccgccgc cgccgccggg atatttttcg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaagcgcgcg gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg 2760

2701 cttcgcgcgc cgcccgccct cagcgacgca acggaagcgg ggcacggggc gaggcgcggc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg 2820

2761 ggagcgcggc gggcggggcc gagactgact ggcgcaatga gggtgtccac tcgcccgccc 2820

.......... .......... .......... .......... ORF 3 ..........

.......... .......... .......... .......... .......... ..........

2821 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctc gtttcttttc 2880

2821 tgccgggaag aggaggcccg acattaatcg cgaaccaaat tactgccgag caaagaaaag 2880

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgtggctgcg tgaaagcctt aaagggctcc gggagggccc tttgtgcggg ggggagcggc 2940

2881 acaccgacgc actttcggaa tttcccgagg ccctcccggg aaacacgccc cccctcgccg 2940

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg 3000

2941 agccccccac gcacgcacac acacacgcac ccctcgcggc gcacgccggg cgcgacgggc 3000

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg 3060

3001 cgccgacact cgcgacgccc gcgccgcgcc ccgaaacacg cgaggcgcac acgcgctccc 3060

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gagcgcggcc gggggcggtg ccccgcggtg cgggggggct gcgaggggaa caaaggctgc 3120

3061 ctcgcgccgg cccccgccac ggggcgccac gcccccccga cgctcccctt gtttccgacg 3120

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa 3180

3121 cacgccccac acacgcaccc ccccactcgt cccccacacc cgcgccgcca gcccgacatt 3180

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cccccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct 3240

3181 ggggggggac gtggggggag gggctcaacg actcgtgccg ggccgaagcc cacgccccga 3240

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ccgtgcgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt 3300

3241 ggcacgcccc gcaccgcgcc ccgagcggca cggcccgccc cccaccgccg tccaccccca 3300

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc 3360

3301 cggcccgccc cgccccggcg gagcccggcc cctcccgagc cccctccccg cgccgccggg 3360

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg 3420

3361 gcctcgcggc cgccgacagc tccgcgccgc tcggcgtcgg taacggaaaa taccattagc 3420

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgcgagaggg cgcagggact tcctttgtcc caaatctggc ggagccgaaa tctgggaggc 3480

3421 acgctctccc gcgtccctga aggaaacagg gtttagaccg cctcggcttt agaccctccg 3480

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa 3540

3481 cggcggcgtg ggggagatcg cccgcgcccc gcttcgccac gccgcggccg tccttccttt 3540

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc 3600

3541 acccgcccct cccggaagca cgcagcggcg cggcggcagg ggaagaggga gaggtcggag 3600

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct 3660

3601 ccccgacagg cgcccccctg ccgacggaag ccccccctgc cccgtcccgc cccaagccga 3660

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt 3720

3661 agaccgcaca ctggccgccg agatctcgga gacgattggt acaagtacgg aagaagaaaa 3720

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat 3780

3721 aggatgtcga ggacccgttg cacgaccaat aacacgacag agtagtaaaa ccgtttctta 3780

.......... .......... pCAG_F_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 taaatttaat taatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg 3840

3781 atttaaatta attagagctg ccatagccaa ttgaaaattt tcttttcccc cctaaccccc 3840

.......... .......... .......... .......... cPPT ..........

.......... .......... .......... .......... .......... ..........

3841 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa tttaaagaat 3900

3841 catgtcacgt cccctttctt atcatctgta ttatcgttgt ctgtatgttt aaatttctta 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgaggttt 3960

3901 atgtttttgt ttaatgtttt taagttttaa aatagctagt gctctgatcg gagctccaaa 3960

.......... .......... .......... .......... .......... ..........

BamHI(3969) ........ .......... .......... .......... ..........

3961 aaactacggg atccaggcct aagcttacgc gtcctagcgc taccggtcgc caccatggtg 4020

3961 tttgatgccc taggtccgga ttcgaatgcg caggatcgcg atggccagcg gtggtaccac 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 4080

4021 tcgttcccgc tcctcgacaa gtggccccac cacgggtagg accagctcga cctgccgctg 4080

.......... .......... EGFP ORF 1 .......... EGFP_N_pri ..........

.......... .......... .......... .......... .......... ..........

4081 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 4140

4081 catttgccgg tgttcaagtc gcacaggccg ctcccgctcc cgctacggtg gatgccgttc 4140

.......... .......... EGFP ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 4200

4141 gactgggact tcaagtagac gtggtggccg ttcgacgggc acgggaccgg gtgggagcac 4200

.......... .......... EGFP ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 4260

4201 tggtgggact ggatgccgca cgtcacgaag tcggcgatgg ggctggtgta cttcgtcgtg 4260

.......... .......... EGFP ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 4320

4261 ctgaagaagt tcaggcggta cgggcttccg atgcaggtcc tcgcgtggta gaagaagttc 4320

.......... .......... EGFP ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 4380

4321 ctgctgccgt tgatgttctg ggcgcggctc cacttcaagc tcccgctgtg ggaccacttg 4380

.......... .......... EGFP ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 4440

4381 gcgtagctcg acttcccgta gctgaagttc ctcctgccgt tgtaggaccc cgtgttcgac 4440

.......... .......... EGFP ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 4500

4441 ctcatgttga tgttgtcggt gttgcagata tagtaccggc tgttcgtctt cttgccgtag 4500

.......... .......... EGFP ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 4560

4501 ttccacttga agttctaggc ggtgttgtag ctcctgccgt cgcacgtcga gcggctggtg 4560

.......... .......... EGFP ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 4620

4561 atggtcgtct tgtgggggta gccgctgccg gggcacgacg acgggctgtt ggtgatggac 4620

.......... .......... EGFP ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 4680

4621 tcgtgggtca ggcgggactc gtttctgggg ttgctcttcg cgctagtgta ccaggacgac 4680

.......... .......... EGFP ORF 1 .......... .......... EGFP_C_pri

.......... .......... .......... .......... .......... ..........

4681 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc 4740

4681 ctcaagcact ggcggcggcc ctagtgagag ccgtacctgc tcgacatgtt caggcctgag 4740

EGFP_C_pri .......... EGFP ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agatctcgac tagctagtag ctagctagct agtcgagctc aagcttcgaa ttcccgggct 4800

4741 tctagagctg atcgatcatc gatcgatcga tcagctcgag ttcgaagctt aagggcccga 4800

.......... ORF 1 .......... .......... .......... ..........

PstI(4803) .......... .......... .......... .......... ..........

4801 gcaggaattc cgcccccccc cccctaacgt tactggccga agccgcttgg aataaggccg 4860

4801 cgtccttaag gcgggggggg ggggattgca atgaccggct tcggcgaacc ttattccggc 4860

.......... .......... .......... IRES .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc 4920

4861 cacacgcaaa cagatataca ataaaaggtg gtataacggc agaaaaccgt tacactcccg 4920

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa 4980

4921 ggcctttgga ccgggacaga agaactgctc gtaaggatcc ccagaaaggg gagagcggtt 4980

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag 5040

4981 tccttacgtt ccagacaact tacagcactt ccttcgtcaa ggagaccttc gaagaacttc 5040

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg 5100

5041 tgtttgttgc agacatcgct gggaaacgtc cgtcgccttg gggggtggac cgctgtccac 5100

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg 5160

5101 ggagacgccg gttttcggtg cacatattct atgtggacgt ttccgccgtg ttggggtcac 5160

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa 5220

5161 ggtgcaacac tcaacctatc aacacctttc tcagtttacc gagaggagtt cgcataagtt 5220

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg 5280

5221 gttccccgac ttcctacggg tcttccatgg ggtaacatac cctagactag accccggagc 5280

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac 5340

5281 cacgtgtacg aaatgtacac aaatcagctc caattttttg cagatccggg gggcttggtg 5340

.......... IRES .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ggggacgtgg ttttcctttg aaaaacacga tgataatacc atggctagat tagataaaag 5400

5341 cccctgcacc aaaaggaaac tttttgtgct actattatgg taccgatcta atctattttc 5400

.......... .......... .......... .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

5401 taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac 5460

5401 atttcactaa ttgtcgcgta atctcgacga attactccag ccttagcttc caaattgttg 5460

.......... .......... ORF 2 TetR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa 5520

5461 ggcatttgag cgggtcttcg atccacatct cgtcggatgt aacataaccg tacatttttt 5520

.......... .......... ORF 2 TetR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt 5580

5521 attcgcccga aacgagctgc ggaatcggta actctacaat ctatccgtgg tatgagtgaa 5580

.......... .......... ORF 2 TetR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag 5640

5581 aacgggaaat cttccccttt cgaccgttct aaaaaatgca ttattgcgat tttcaaaatc 5640

.......... .......... ORF 2 TetR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga 5700

5641 tacacgaaat gattcagtag cgctacctcg ttttcatgta aatccatgtg ccggatgtct 5700

.......... .......... ORF 2 TetR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact 5760

5701 ttttgtcata ctttgagagc ttttagttaa tcggaaaaat acggttgttc caaaaagtga 5760

.......... .......... ORF 2 TetR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga 5820

5761 tctcttacgt aatatacgtg agtcgcgaca ccccgtaaaa tgaaatccaa cgcataacct 5820

.......... .......... ORF 2 TetR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc 5880

5821 tctagttctc gtagttcagc gatttcttct ttccctttgt ggatgatgac tatcatacgg 5880

.......... .......... ORF 2 TetR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt 5940

5881 cggtaataat gctgttcgat agcttaataa actagtggtt ccacgtctcg gtcggaagaa 5940

.......... .......... ORF 2 TetR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc 6000

5941 taagccggaa cttaactagt atacgcctaa tctttttgtt gaatttacac tttcacccag 6000

.......... .......... ORF 2 TetR .......... .......... ..........

.......... SalI(6020) .......... .......... .......... ..........

6001 gccaaaaaag aagagaaagg tcgacggcgg tggtgctttg tctcctcagc actctgctgt 6060

6001 cggttttttc ttctctttcc agctgccgcc accacgaaac agaggagtcg tgagacgaca 6060

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 cactcaagga agtatcatca agaacaagga gggcatggat gctaagtcac taactgcctg 6120

6061 gtgagttcct tcatagtagt tcttgttcct cccgtaccta cgattcagtg attgacggac 6120

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gtcccggaca ctggtgacct tcaaggatgt atttgtggac ttcaccaggg aggagtggaa 6180

6121 cagggcctgt gaccactgga agttcctaca taaacacctg aagtggtccc tcctcacctt 6180

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gctgctggac actgctcagc agatcgtgta cagaaatgtg atgctggaga actataagaa 6240

6181 cgacgacctg tgacgagtcg tctagcacat gtctttacac tacgacctct tgatattctt 6240

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 cctggtttcc ttgggttatc agcttactaa gccagatgtg atcctccggt tggagaaggg 6300

6241 ggaccaaagg aacccaatag tcgaatgatt cggtctacac taggaggcca acctcttccc 6300

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 agaagagccc tggctggtgg agagagaaat tcaccaagag acccatcctg attcagagac 6360

6301 tcttctcggg accgaccacc tctctcttta agtggttctc tgggtaggac taagtctctg 6360

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 tgcatttgaa atcaaatcat cagtttaagg atctcgacta gctagtagct agctagctag 6420

6361 acgtaaactt tagtttagta gtcaaattcc tagagctgat cgatcatcga tcgatcgatc 6420

.......... ORF 2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 tcgagctcaa cttcgaattc gatgggacta gtcatatgat aatcaacctc tggattacaa 6480

6421 agctcgagtt gaagcttaag ctaccctgat cagtatacta ttagttggag acctaatgtt 6480

.......... .......... .......... .......... .......... WPRE

.......... .......... .......... .......... .......... ..........

6481 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 6540

6481 ttaaacactt tctaactgac cataagaatt gatacaacga ggaaaatgcg atacacctat 6540

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 6600

6541 gcgacgaaat tacggaaaca tagtacgata acgaagggca taccgaaagt aaaagaggag 6600

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 6660

6601 gaacatattt aggaccaacg acagagaaat actcctcaac accgggcaac agtccgttgc 6660

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 6720

6661 accgcaccac acgtgacaca aacgactgcg ttgggggtga ccaaccccgt aacggtggtg 6720

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 6780

6721 gacagtcgag gaaaggccct gaaagcgaaa gggggaggga taacggtgcc gccttgagta 6780

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 6840

6781 gcggcggacg gaacgggcga cgacctgtcc ccgagccgac aacccgtgac tgttaaggca 6840

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 6900

6841 ccacaacagc cccttcgact gcaggaaagg taccgacgag cggacacaac ggtggaccta 6900

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 6960

6901 agacgcgccc tgcaggaaga cgatgcaggg aagccgggag ttaggtcgcc tggaaggaag 6960

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 7020

6961 ggcgccggac gacggccgag acgccggaga aggcgcagaa gcggaagcgg gagtctgctc 7020

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... MscI(7063) ..........

7021 tcggatctcc ctttgggccg cctccccgca tcggtacgta tggccaggta cctttaagac 7080

7021 agcctagagg gaaacccggc ggaggggcgt agccatgcat accggtccat ggaaattctg 7080

.......... WPRE .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 7140

7081 gttactgaat gttccgtcga catctagaat cggtgaaaaa ttttcttttc ccccctgacc 7140

.......... .......... .......... .......... cPPT U3PPT ..........

.......... .......... .......... .......... .......... ..........

7141 aagggctaat tcactcccaa cgaagacaag atctcgagtt taccactccc tatcagtgat 7200

7141 ttcccgatta agtgagggtt gcttctgttc tagagctcaa atggtgaggg atagtcacta 7200

.......... .......... .......... .......... TRE ..........

.......... .......... .......... .......... .......... ..........

7201 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg 7260

7201 tctcttttca ctttcagctc aaatggtgag ggatagtcac tatctctttt cactttcagc 7260

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc 7320

7261 tcaaatggtg agggatagtc actatctctt ttcactttca gctcaaatgg tgagggatag 7320

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga 7380

7321 tcactatctc ttttcacttt cagctcaaat ggtgagggat agtcactatc tcttttcact 7380

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc 7440

7381 ttcagctcaa atggtgaggg atagtcacta tctcttttca ctttcagctc aaatggtgag 7440

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 cctatcagtg atagagaaaa gtgaaagtcg gatcgatctg ctttttgctt gtactgggtc 7500

7441 ggatagtcac tatctctttt cactttcagc ctagctagac gaaaaacgaa catgacccag 7500

TRE .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 7560

7501 agagaccaat ctggtctaga ctcggaccct cgagagaccg attgatccct tgggtgacga 7560

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 7620

7561 attcggagtt atttcgaacg gaactcacga agttcatcac acacgggcag acaacacact 7620

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat 7680

7621 gagaccattg atctctaggg agtctgggaa aatcagtcac accttttaga gatcgtcgta 7680

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag 7740

7681 gatcttaatt aaggcacata agatatcaca gtggatttag catacacata ctatgtattc 7740

.......... .......... Sp6_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca 7800

7741 caatacataa ttaacatcgg cgcaagattg ctgttataca tgttcggatt aacacatcgt 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg 7860

7801 agaccgaatg acttcgtctg ggatagtaga gagagcattt gacggcagtc tcagccaaac 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa 7920

7861 caacctgctt ggaagactca aagaccattg cggcagggcg tgggccttta ccagtcgctt 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc 7980

7921 ggttagtcgt cccagtagcg atcggtctag gagatgcggc ctgcgtagca ccggccgtag 7980

.......... .......... .......... .......... tet ..........

.......... .......... .......... .......... .......... ..........

7981 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat 8040

7981 tggccgcggt gtccacgcca acgaccgcgg atatagcggc tgtagtggct accccttcta 8040

.......... ........ tet pBRrevBam_pri ........ ..........

.......... .......... .......... .......... .......... ..........

8041 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc 8100

8041 gcccgagcgg tgaagcccga gtactcgcga acaaagccgc acccatacca ccgtccgggg 8100

.......... .......... tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg 8160

8101 caccggcccc ctgacaaccc gcggtagagg aacgtacgtg gtaaggaacg ccgccgccac 8160

.......... .......... tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag 8220

8161 gagttgccgg agttggatga tgacccgacg aaggattacg tcctcagcgt attccctctc 8220

.......... .......... tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg 8280

8221 gcagcttacc acgtgagagt catgttagac gagactacgg cgtatcaatt cggtcggggc 8280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 8340

8281 tgtgggcggt tgtgggcgac tgcgcgggac tgcccgaaca gacgagggcc gtaggcgaat 8340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 8400

8341 gtctgttcga cactggcaga ggccctcgac gtacacagtc tccaaaagtg gcagtagtgg 8400

.......... .......... .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

8401 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat 8460

8401 ctttgcgcgc tctgctttcc cggagcacta tgcggataaa aatatccaat tacagtacta 8460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 8520

8461 ttattaccaa agaatctgca gtccaccgtg aaaagcccct ttacacgcgc cttggggata 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 8580

8521 aacaaataaa aagatttatg taagtttata cataggcgag tactctgtta ttgggactat 8580

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

8581 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 8640

8581 ttacgaagtt attataactt tttccttctc atactcataa gttgtaaagg cacagcggga 8640

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

8641 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 8700

8641 ataagggaaa aaacgccgta aaacggaagg acaaaaacga gtgggtcttt gcgaccactt 8700

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 8760

8701 tcattttcta cgacttctag tcaacccacg tgctcaccca atgtagcttg acctagagtt 8760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 8820

8761 gtcgccattc taggaactct caaaagcggg gcttcttgca aaaggttact actcgtgaaa 8820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 8880

8821 atttcaagac gatacaccgc gccataatag ggcataactg cggcccgttc tcgttgagcc 8880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 8940

8881 agcggcgtat gtgataagag tcttactgaa ccaactcatg agtggtcagt gtcttttcgt 8940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 9000

8941 agaatgccta ccgtactgtc attctcttaa tacgtcacga cggtattggt actcactatt 9000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 9060

9001 gtgacgccgg ttgaatgaag actgttgcta gcctcctggc ttcctcgatt ggcgaaaaaa 9060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 9120

9061 cgtgttgtac cccctagtac attgagcgga actagcaacc cttggcctcg acttacttcg 9120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... FspI(9176)

9121 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 9180

9121 gtatggtttg ctgctcgcac tgtggtgcta cggacatcgt taccgttgtt gcaacgcgtt 9180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 9240

9181 tgataattga ccgcttgatg aatgagatcg aagggccgtt gttaattatc tgacctacct 9240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 9300

9241 ccgcctattt caacgtcctg gtgaagacgc gagccgggaa ggccgaccga ccaaataacg 9300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 9360

9301 actatttaga cctcggccac tcgcacccag agcgccatag taacgtcgtg accccggtct 9360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 9420

9361 accattcggg agggcatagc atcaatagat gtgctgcccc tcagtccgtt gatacctact 9420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 9480

9421 tgctttatct gtctagcgac tctatccacg gagtgactaa ttcgtaacca ttgacagtct 9480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 9540

9481 ggttcaaatg agtatatatg aaatctaact aaattttgaa gtaaaaatta aattttccta 9540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 9600

9541 gatccacttc taggaaaaac tattagagta ctggttttag ggaattgcac tcaaaagcaa 9600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 9660

9601 ggtgactcgc agtctggggc atcttttcta gtttcctaga agaactctag gaaaaaaaga 9660

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

9661 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 9720

9661 cgcgcattag acgacgaacg tttgtttttt tggtggcgat ggtcgccacc aaacaaacgg 9720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9721 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 9780

9721 cctagttctc gatggttgag aaaaaggctt ccattgaccg aagtcgtctc gcgtctatgg 9780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9781 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 9840

9781 tttatgacag gaagatcaca tcggcatcaa tccggtggtg aagttcttga gacatcgtgg 9840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9841 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 9900

9841 cggatgtatg gagcgagacg attaggacaa tggtcaccga cgacggtcac cgctattcag 9900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9901 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 9960

9901 cacagaatgg cccaacctga gttctgctat caatggccta ttccgcgtcg ccagcccgac 9960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9961 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 10020

9961 ttgcccccca agcacgtgtg tcgggtcgaa cctcgcttgc tggatgtggc ttgactctat 10020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

10021 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 10080

10021 ggatgtcgca ctcgtaactc tttcgcggtg cgaagggctt ccctctttcc gcctgtccat 10080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

10081 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 10140

10081 aggccattcg ccgtcccagc cttgtcctct cgcgtgctcc ctcgaaggtc cccctttgcg 10140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

10141 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 10200

10141 gaccatagaa atatcaggac agcccaaagc ggtggagact gaactcgcag ctaaaaacac 10200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

10201 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 10260

10201 tacgagcagt ccccccgcct cggatacctt tttgcggtcg ttgcgccgga aaaatgccaa 10260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

10261 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 10320

10261 ggaccggaaa acgaccggaa aacgagtgta caagaaagga cgcaataggg gactaagaca 10320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 10380

10321 cctattggca taatggcgga aactcactcg actatggcga gcggcgtcgg cttgctggct 10380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 10440

10381 cgcgtcgctc agtcactcgc tccttcgcct tctcgcgggt tatgcgtttg gcggagaggg 10440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag 10500

10441 gcgcgcaacc ggctaagtaa ttacgtcgac accttacaca cagtcaatcc cacacctttc 10500

.......... .......... .......... ..... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

10501 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 10560

10501 aggggtccga ggggtcgtcc gtcttcatac gtttcgtacg tagagttaat cagtcgttgg 10560

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 10620

10561 tccacacctt tcaggggtcc gaggggtcgt ccgtcttcat acgtttcgta cgtagagtta 10620

.......... ...... SV40_enh SV40_pro ...... .......... SV40_pro

.......... .......... .......... .......... .......... ..........

10621 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 10680

10621 atcagtcgtt ggtatcaggg cggggattga ggcgggtagg gcggggattg aggcgggtca 10680

.......... .. SV40_enh SV40_pro SV40_pro . SV40_origin .........

.......... .......... .......... .......... .......... ..........

10681 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 10740

10681 aggcgggtaa gaggcggggt accgactgat taaaaaaaat aaatacgtct ccggctccgg 10740

SV40_enh SV40_pro SV40_pro SV40_origin SV40pro_F_pri ......

.......... .......... .......... .......... .......... ..........

10741 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 10800

10741 cggagccgga gactcgataa ggtcttcatc actcctccga aaaaacctcc ggatccgaaa 10800

SV40_pro .......... SV40_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10801 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc 10860

10801 acgtttttcg aacctgtgtt ctgtccgaac gctctataca aactcttatg gtgaaatagg 10860

SV40_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10861 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag 10920

10861 gcgcagtccc tctccgtcac gcatttttct gcgcctgagt acactttatg accaaaaatc 10920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10921 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt 10980

10921 acgcggtcta gagatattag agcgcgttgg ataaaagggg agcttgtgaa aaattcggca 10980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10981 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt 11040

10981 tctatttgtc cgaccctgtg aagtgtactc gctttttatg tagcagtgga ccctgtacaa 11040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11041 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat 11100

11041 cgtctaggta cgtgcatttg agcgttcggc tgactacgga agacttgtta cctttccgta 11100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11101 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt 11160

11101 ataacggcat tcggcaccgc cagacatggc ccacgcaatg accgcgcact tgacccataa 11160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11161 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa 11220

11161 gcagtacagc tatggcaaac ataaaggtcg atgctagtgc tgttggtcgc gctcgaattt 11220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11221 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat 11280

11221 cacgactttg cgcgtcttcc gctaccgctt ccgaagtagc aataactact ggaccaccta 11280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11281 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc 11340

11281 tggccaccat gacgccaacg ctaagcactt tacataggtt ttcgcgtgaa acagtggtag 11340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11341 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat 11400

11341 aagcgttttg gccgaccagc aggcgaccaa ctactgatac aacaactata gggcgttcta 11400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11401 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc 11460

11401 tggacctaac ttgtcggcac cctatacccg cagcataagc agggcggtta gaggccagcg 11460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11461 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca 11520

11461 attagaaaag ttgcggaccg tgacggcccg caacaagaaa aattgaagtc cgcccaatgt 11520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11521 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac 11580

11521 tatcaaaggt cattcataag acctccgacg taggtactgt gtccgtttgg actcgctttg 11580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11581 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca 11640

11581 ggacaagttt ggggcgaaat ttgtaggact ttggagctgc gatcaggcgg cgaaattagt 11640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11641 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg 11700

11641 gccgcgtgtt ggcggacacg tcagccggga actaccattt tggtagggag tgaccatagc 11700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11701 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt 11760

11701 gtactaattg gcagactaca cctagaccgc gccgtaactg ggtgcgcttt aggagctgca 11760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11761 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt 11820

11761 ggtccgtgca taacactact cgctacggct tgcatggctg ctactaaata tgctatgcca 11820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11821 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac 11880

11821 ctaaccgatg gcaccgccgt tgacctaaat actcacccgg ggcctagaaa cacttccttg 11880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11881 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg 11940

11881 gaatgaagac accacactgt attaacctgt ttgatggatg tctctaaatt tcgagattcc 11940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11941 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat 12000

11941 atttatattt taaaaattca catattacac aatttgatga ctaagattaa caaacacata 12000

SV40_int .......... ........ SV40_3_splice ........ ..........

.......... .......... .......... .......... .......... ..........

12001 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg 12060

12001 aaatctaagg ttggatacct tgactactta ccctcgtcac caccttacgg aaattactcc 12060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12061 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc 12120

12061 ttttggacaa aacgagtctt ctttacggta gatcactact actccgatga cgactgagag 12120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12121 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag 12180

12121 ttgtaagatg aggaggtttt ttcttctctt tccatcttct ggggttcctg aaaggaagtc 12180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12181 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta 12240

12181 ttaacgattc aaaaaactca gtacgacaca aatcattatc ttgagaacga acgaaacgat 12240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12241 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg 12300

12241 aaatgtggtg tttccttttt cgacgtgacg atatgttctt ttaatacctt tttataagac 12300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12301 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac 12360

12301 attggaaata ttcatccgta ttgtcaatat tagtattgta tgacaaaaaa gaatgaggtg 12360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12361 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt 12420

12361 tgtccgtatc tcacagacga taattattga tacgagtttt taacacatgg aaatcgaaaa 12420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12421 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata 12480

12421 attaaacatt tccccaatta ttccttataa actacatatc acggaactga tctctagtat 12480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12481 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 12540

12481 tagtcggtat ggtgtaaaca tctccaaaat gaacgaaatt ttttggaggg tgtggagggg 12540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12541 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 12600

12541 gacttggact ttgtatttta cttacgttaa caacaacaat tgaacaaata acgtcgaata 12600

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

12601 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 12660

12601 ttaccaatgt ttatttcgtt atcgtagtgt ttaaagtgtt tatttcgtaa aaaaagtgac 12660

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

12661 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg 12720

12661 gtaagatcaa caccaaacag gtttgagtag ttacatagaa tagtacagac ctagttgacc 12720

........ EBV_rev_pri SV40_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

12721 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc 12780

12721 tattgagttc gattggtttt agtagggttt gaagggtggg gtatgggata atggtgacgg 12780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12781 aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta 12840

12781 ttaatggatc accaaagtaa atgagatttg gacactaagg agacttaata aaagtaaaat 12840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12841 aagaaattgt atttgttaaa tatgtactac aaactta... .......... .......... 12877

12841 ttctttaaca taaacaattt atacatgatg tttgaat... .......... .......... 12877

.......... .......... .......... .......... .......... ..........