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    Plasmid map of pLVTH

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    Gene/insert name: None
    Insert size (bp): Unknown
    Vector backbone: pLVTH
    Type of vector: Mammalian expression,RNAi
    Backbone size (bp): 11086
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: No
    Bacterial strain for growth and growth condition: Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
    Plasmid Provided In: Stbl3
    Principal Investigator: Didier Trono
    Comments: This vector has been replaced by the newer pLVTHM vector, which allows for direct cloning of shRNA.
    For pLVTH: if you have your shRNA already in pSUPER (or any other plasmid under control of PolIII promoter) you may use EcoR1-Cla1 sites to replace H1 promoter in pLVTH with H1-shRNA cassette from pSUPER (or other plasmid). Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation. This plasmid needs to be grown in a Dam+ bacteria strain if you wish to use ClaI for cloning.
    Article: Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. Wiznerowicz M et al. (J Virol. 2003 Aug . 77(16):8957-61. Pubmed)

    image of pLVTH

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