Sequence of pLVTHM

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

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Sequence of pLVTHM

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Sequence of pLVTHM : 11085 BP; 2806 A; 2685 C; 2875 G; 2719 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac 60

1 aaccttcccg attaagtgag ggtttcttct gttctatagg aactagacac ctagatggtg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 120

61 tgtgttccga tgaagggact aatcgtcttg atgtgtggtc ccggtcccca gtctataggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 180

121 gactggaaac ctaccacgat gttcgatcat ggtcaactcg gtctattcca tcttctccgg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 240

181 ttatttcctc tcttgtggtc gaacaatgtg ggacactcgg acgtacccta cctactgggc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... PmlI(291)

241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 300

241 ctctctcttc acaatctcac ctccaaactg tcggcggatc gtaaagtagt gcaccgggct 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 360

301 ctcgacgtag gcctcatgaa gttcttgacg actatagctc gaacgatgtt ccctgaaagg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 420

361 cgacccctga aaggtccctc cgcaccggac ccgccctgac ccctcaccgc tcgggagtct 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 480

421 aggacgtata ttcgtcgacg aaaaacggac atgacccaga gagaccaatc tggtctagac 480

.......... .......... .......... . HIV-1_5_LTR truncHIV-1_3_LTR .

.......... .......... .......... .......... .......... ..........

481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 540

481 tcggaccctc gagagaccga ttgatccctt gggtgacgaa ttcggagtta tttcgaacgg 540

.......... . HIV-1_5_LTR truncHIV-1_3_LTR . .......... ..........

.......... .......... .......... .......... .......... ..........

541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 600

541 aactcacgaa gttcatcaca cacgggcaga caacacactg agaccattga tctctaggga 600

.......... . HIV-1_5_LTR truncHIV-1_3_LTR . .......... ..........

.......... .......... .......... .......... .......... ..........

601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 660

601 gtctgggaaa atcagtcaca ccttttagag atcgtcaccg cgggcttgtc cctgaacttt 660

. HIV-1_5_LTR truncHIV-1_3_LTR . .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 720

661 cgctttccct ttggtctcct cgagagagct gcgtcctgag ccgaacgact tcgcgcgtgc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 780

721 cgttctccgc tccccgccgc tgaccactca tgcggttttt aaaactgatc gcctccgatc 780

.......... .......... .......... ........ HIV-1_psi_pack ........

.......... .......... .......... .......... .......... ..........

781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 840

781 ttcctctctc tacccacgct ctcgcagtca taattcgccc cctcttaatc tagcgctacc 840

HIV-1_psi_pack ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 900

841 cttttttaag ccaattccgg tccccctttc ttttttatat ttaattttgt atatcatacc 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 960

901 cgttcgtccc tcgatcttgc taagcgtcaa ttaggaccgg acaatctttg tagtcttccg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 1020

961 acatctgttt atgaccctgt cgatgttggt agggaagtct gtcctagtct tcttgaatct 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac 1080

1021 agtaatatat tatgtcatcg ttgggagata acacacgtag tttcctatct ctattttctg 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 1140

1081 tggttccttc gaaatctgtt ctatctcctt ctcgttttgt tttcattctg gtggcgtgtc 1140

.......... .......... .......... .......... .......... ..........

NotI(1145) .......... EagI(1170) .......... .......... ..........

1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga 1200

1141 gttcgccggc gactagaagt ctggacctcc tcctctatac tccctgttaa cctcttcact 1200

.......... .......... .......... ......... Orf frame 2 .........

.......... .......... .......... .......... .......... ..........

1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa 1260

1201 taatatattt atatttcatc atttttaact tggtaatcct catcgtgggt ggttccgttt 1260

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt t ccttgggtt 1320

1261 ctcttctcac cacgtctctc ttttttctcg tcacccttat cctcgaaaca aggaacccaa 1320

.......... ......... Orf frame 2 ......... RRE ..........

.......... .......... .......... .......... .......... ..........

1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag 1380

1321 gaaccctcgt cgtccttcgt gatacccgcg tcgcagttac tgcgactgcc atgtccggtc 1380

.......... ....... Orf frame 2 RRE ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca 1440

1381 tgttaataac agaccatatc acgtcgtcgt cttgttaaac gactcccgat aactccgcgt 1440

.......... ....... Orf frame 2 RRE ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc 1500

1441 tgtcgtagac aacgttgagt gtcagacccc gtagttcgtc gaggtccgtt cttaggaccg 1500

.......... ....... Orf frame 2 RRE ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact 1560

1501 acacctttct atggatttcc tagttgtcga ggacccctaa accccaacga gaccttttga 1560

......... RRE Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat 1620

1561 gtaaacgtgg tgacgacacg gaaccttacg atcaacctca ttatttagag accttgtcta 1620

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat 1680

1621 aaccttagtg tgctggacct acctcaccct gtctctttaa ttgttaatgt gttcgaatta 1680

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga 1740

1681 tgtgaggaat taacttctta gcgttttggt cgttcttttc ttacttgttc ttaataacct 1740

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat 1800

1741 taatctattt acccgttcaa acaccttaac caaattgtat tgtttaaccg acaccatata 1800

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact 1860

1801 ttttaataag tattactatc atcctccgaa ccatccaaat tcttatcaaa aacgacatga 1860

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc 1920

1861 aagatatcac ttatctcaat ccgtccctat aagtggtaat agcaaagtct gggtggaggg 1920

.......... ......... Orf frame 2 ......... ......... Orf frame 1

.......... .......... .......... .......... .......... ..........

1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag 1980

1921 ttggggctcc cctgggctgt ccgggcttcc ttatcttctt cttccacctc tctctctgtc 1980

.......... ... Orf frame 2 Orf frame 1 ... .......... ..........

.......... .......... .......... .......... SalI(2027) ..........

1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgcgatgtcg acgataagct 2040

1981 tctgtctagg taagctaatc acttgcctag agctgccata gcgctacagc tgctattcga 2040

.......... ... Orf frame 2 Orf frame 1 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag 2100

2041 aacgtttcta cctatttcaa aatttgtctc tccttagaaa cgtcgattac ctggaagatc 2100

Orf frame 2 ....... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc 2160

2101 cagaactttc ctcaccctta accgaggcca cgggcagtca cccgtctcgc gtgtagcggg 2160

.......... ......... Orf frame 1 ....... EF1a_promoter ........

.......... .......... .......... .......... .......... ..........

2161 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg 2220

2161 tgtcaggggc tcttcaaccc ccctccccag ccgttaactt ggccacggat ctcttccacc 2220

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 2280

2221 gcgccccatt tgaccctttc actacagcac atgaccgagg cggaaaaagg gctcccaccc 2280

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 2340

2281 cctcttggca tatattcacg tcatcagcgg cacttgcaag aaaaagcgtt gcccaaacgg 2340

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat 2400

2341 cggtcttgtg tccattcacg gcacacacca agggcgcccg gaccggagaa atgcccaata 2400

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggcccttgcg tgccttgaat tacttccact ggctgcagta cgtgattctt gatcccgagc 2460

2401 ccgggaacgc acggaactta atgaaggtga ccgacgtcat gcactaagaa ctagggctcg 2460

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc 2520

2461 aagcccaacc ttcacccacc ctctcaagct ccggaacgcg aattcctcgg ggaagcggag 2520

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc 2580

2521 cacgaactca actccggacc ggacccgcga ccccggcggc gcacgcttag accaccgtgg 2580

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg 2640

2581 aagcgcggac agagcgacga aagctattca gagatcggta aattttaaaa actactggac 2640

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg 2700

2641 gacgctgcga aaaaaagacc gttctatcag aacatttacg cccggttcta gacgtgtgac 2700

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt 2760

2701 cataaagcca aaaaccccgg cgcccgccgc tgccccgggc acgcagggtc gcgtgtacaa 2760

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg 2820

2761 gccgctccgc cccggacgct cgcgccggtg gctcttagcc tgcccccatc agagttcgac 2820

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa 2880

2821 cggccggacg agaccacgga ccggagcgcg gcggcacata gcggggcggg acccgccgtt 2880

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg 2940

2881 ccgaccgggc cagccgtggt caacgcactc gcctttctac cggcgaaggg ccgggacgac 2940

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac 3000

2941 gtccctcgag ttttacctcc tgcgccgcga gccctctcgc ccgcccactc agtgggtgtg 3000

.......... .. Orf frame 1 EF1a_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg 3060

3001 tttccttttc ccggaaaggc aggagtcggc agcgaagtac actgaggtgc ctcatggccc 3060

....... Orf frame 1 EF1a_promoter ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg 3120

3061 gcggcaggtc cgtggagcta atcaagagct cgaaaacctc atgcagcaga aatccaaccc 3120

.......... ........ EF1a_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc 3180

3121 ccctccccaa aatacgctac ctcaaagggg tgtgactcac ccacctctga cttcaatccg 3180

.......... ........ EF1a_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt 3240

3181 gtcgaaccgt gaactacatt aagaggaacc ttaaacggga aaaactcaaa cctagaacca 3240

.......... ........ EF1a_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag 3300

3241 agtaagagtt cggagtctgt caccaagttt caaaaaaaga aggtaaagtc cacagcactc 3300

..... EF1a_fwd_primer EF1a_promoter ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gaatttcgac atttaaattt aattaatctc gacggtatcg gttaactttt aaaagaaaag 3360

3301 cttaaagctg taaatttaaa ttaattagag ctgccatagc caattgaaaa ttttcttttc 3360

.......... .......... .......... .......... .......... cPPT

.......... .......... .......... .......... .......... ..........

3361 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 3420

3361 ccccctaacc ccccatgtca cgtccccttt cttatcatct gtattatcgt tgtctgtatg 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttccgatc acgagactag 3480

3421 tttgatttct taatgttttt gtttaatgtt tttaagtttt aaaaggctag tgctctgatc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cctcgaggtt taaactacgg gatcttcgaa ggcctaagct tacgcgcgcg tcctagcgct 3540

3481 ggagctccaa atttgatgcc ctagaagctt ccggattcga atgcgcgcgc aggatcgcga 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 3600

3541 tggccagcgg tggtaccact cgttcccgct cctcgacaag tggccccacc acgggtagga 3600

.......... .......... ....... EGFP Orf frame 2 ....... ..........

.......... .......... .......... .......... .......... ..........

3601 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 3660

3601 ccagctcgac ctgccgctgc atttgccggt gttcaagtcg cacaggccgc tcccgctccc 3660

EGFP_N_primer ... EGFP Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 3720

3661 gctacggtgg atgccgttcg actgggactt caagtagacg tggtggccgt tcgacgggca 3720

.......... ....... EGFP Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 3780

3721 cgggaccggg tgggagcact ggtgggactg gatgccgcac gtcacgaagt cggcgatggg 3780

.......... ....... EGFP Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 3840

3781 gctggtgtac ttcgtcgtgc tgaagaagtt caggcggtac gggcttccga tgcaggtcct 3840

.......... ....... EGFP Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 3900

3841 cgcgtggtag aagaagttcc tgctgccgtt gatgttctgg gcgcggctcc acttcaagct 3900

.......... ....... EGFP Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 3960

3901 cccgctgtgg gaccacttgg cgtagctcga cttcccgtag ctgaagttcc tcctgccgtt 3960

.......... ....... EGFP Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 4020

3961 gtaggacccc gtgttcgacc tcatgttgat gttgtcggtg ttgcagatat agtaccggct 4020

.......... ....... EGFP Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 4080

4021 gttcgtcttc ttgccgtagt tccacttgaa gttctaggcg gtgttgtagc tcctgccgtc 4080

.......... ....... EGFP Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 4140

4081 gcacgtcgag cggctggtga tggtcgtctt gtgggggtag ccgctgccgg ggcacgacga 4140

.......... ....... EGFP Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 4200

4141 cgggctgttg gtgatggact cgtgggtcag gcgggactcg tttctggggt tgctcttcgc 4200

.......... ....... EGFP Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 4260

4201 gctagtgtac caggacgacc tcaagcactg gcggcggccc tagtgagagc cgtacctgct 4260

..... EGFP_C_primer EGFP Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gctgtacaag tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa 4320

4261 cgacatgttc aggcctgagt ctagagctat cgatcatcga tcgatcgatc agctcgagtt 4320

EGFP Orf frame 2 ........ .......... .......... ..........

SpeI(4329) NdeI(4336) .......... .......... .......... ..........

4321 cttcggggac tagtcatatg ataatcaacc tctggattac aaaatttgtg aaagattgac 4380

4321 gaagcccctg atcagtatac tattagttgg agacctaatg ttttaaacac tttctaactg 4380

.......... .......... .......... .......... WPRE ..........

.......... .......... .......... .......... .......... ..........

4381 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 4440

4381 accataagaa ttgatacaac gaggaaaatg cgatacacct atgcgacgaa attacggaaa 4440

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 4500

4441 catagtacga taacgaaggg cataccgaaa gtaaaagagg aggaacatat ttaggaccaa 4500

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 4560

4501 cgacagagaa atactcctca acaccgggca acagtccgtt gcaccgcacc acacgtgaca 4560

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 4620

4561 caaacgactg cgttgggggt gaccaacccc gtaacggtgg tggacagtcg aggaaaggcc 4620

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 4680

4621 ctgaaagcga aagggggagg gataacggtg ccgccttgag tagcggcgga cggaacgggc 4680

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 4740

4681 gacgacctgt ccccgagccg acaacccgtg actgttaagg caccacaaca gccccttcga 4740

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 4800

4741 ctgcaggaaa ggtaccgacg agcggacaca acggtggacc taagacgcgc cctgcaggaa 4800

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 4860

4801 gacgatgcag ggaagccggg agttaggtcg cctggaagga agggcgccgg acgacggccg 4860

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 4920

4861 agacgccgga gaaggcgcag aagcggaagc gggagtctgc tcagcctaga gggaaacccg 4920

.......... .......... WPRE .......... .......... ..........

.......... .......... MscI(4945) KpnI(4953) .......... ..........

4921 cgcctccccg catcggtacg tatggccagg tacctttaag accaatgact tacaaggcag 4980

4921 gcggaggggc gtagccatgc ataccggtcc atggaaattc tggttactga atgttccgtc 4980

WPRE .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 5040

4981 gacatctaga atcggtgaaa aattttcttt tcccccctga ccttcccgat taagtgaggg 5040

.......... .......... cPPT U3PPT .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aacgaagaca agatgggatc aattcaccat gctagtggat ccccctcgag tttaccactc 5100

5041 ttgcttctgt tctaccctag ttaagtggta cgatcaccta gggggagctc aaatggtgag 5100

.......... .......... .......... .......... .......... TRE

.......... .......... .......... .......... .......... ..........

5101 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa 5160

5101 ggatagtcac tatctctttt cactttcagc tcaaatggtg agggatagtc actatctctt 5160

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta 5220

5161 ttcactttca gctcaaatgg tgagggatag tcactatctc ttttcacttt cagctcaaat 5220

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat 5280

5221 ggtgagggat agtcactatc tcttttcact ttcagctcaa atggtgaggg atagtcacta 5280

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg 5340

5281 tctcttttca ctttcagctc aaatggtgag ggatagtcac tatctctttt cactttcagc 5340

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agtttaccac tccctatcag tgatagagaa aagtgaaagt cggggctgca ggaattcgaa 5400

5341 tcaaatggtg agggatagtc actatctctt ttcactttca gccccgacgt ccttaagctt 5400

.......... TRE .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cgctgacgtc atcaacccgc tccaaggaat cgcgggccca gtgtcactag gcgggaacac 5460

5401 gcgactgcag tagttgggcg aggttcctta gcgcccgggt cacagtgatc cgcccttgtg 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg acaggggagt ggcgccctgc 5520

5461 ggtcgcgcgc acgcgggacc gtccttctac cgacactccc tgtcccctca ccgcgggacg 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat gtctttggat 5580

5521 ttataaacgt acagcgatac acaagaccct ttagtggtat ttgcacttta cagaaaccta 5580

.......... .......... H1_primer .......... .......... ..........

.......... .......... .......... .......... ClaI(5628) ..........

5581 ttgggaatct tataagttct gtatgagacc acgcgtctcc aagcttatcg ataccgtcgc 5640

5581 aacccttaga atattcaaga catactctgg tgcgcagagg ttcgaatagc tatggcagcg 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 atgggaataa cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg 5700

5641 tacccttatt gaagcatatc gtatgtaata tgcttcaata cgacgaaaaa cgaacatgac 5700

.......... .......... loxP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 5760

5701 ccagagagac caatctggtc tagactcgga ccctcgagag accgattgat cccttgggtg 5760

.......... . HIV-1_5_LTR truncHIV-1_3_LTR . .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 5820

5761 acgaattcgg agttatttcg aacggaactc acgaagttca tcacacacgg gcagacaaca 5820

.......... . HIV-1_5_LTR truncHIV-1_3_LTR . .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 5880

5821 cactgagacc attgatctct agggagtctg ggaaaatcag tcacaccttt tagagatcgt 5880

.......... . HIV-1_5_LTR truncHIV-1_3_LTR . .......... ..........

XbaI(5884) .......... .......... .......... .......... ..........

5881 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca 5940

5881 cgtagatctt aattaaggca cataagatat cacagtggat ttagcataca catactatgt 5940

.......... .......... .......... Sp6_primer .......... ..........

.......... .......... .......... .......... .......... ..........

5941 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt 6000

5941 attccaatac ataattaaca tcggcgcaag attgctgtta tacatgttcg gattaacaca 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg 6060

6001 tcgtagaccg aatgacttcg tctgggatag tagagagagc atttgacggc agtctcagcc 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag 6120

6061 aaaccaacct gcttggaaga ctcaaagacc attgcggcag ggcgtgggcc tttaccagtc 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg 6180

6121 gcttggttag tcgtcccagt agcgatcggt ctaggagatg cggcctgcgt agcaccggcc 6180

.......... .......... .......... ......... tet(564-300) .........

.......... .......... .......... .......... .......... ..........

6181 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga 6240

6181 gtagtggccg cggtgtccac gccaacgacc gcggatatag cggctgtagt g gctacccct 6240

.......... ......... tet(564-300) ...... pBRrevBam_primer .......

.......... .......... .......... .......... .......... ..........

6241 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg 6300

6241 tctagcccga gcggtgaagc ccgagtactc gcgaacaaag ccgcacccat accaccgtcc 6300

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc 6360

6301 ggggcaccgg ccccctgaca acccgcggta gaggaacgta cgtggtaagg aacgccgccg 6360

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg 6420

6361 ccacgagttg ccggagttgg atgatgaccc gacgaaggat tacgtcctca gcgtattccc 6420

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc 6480

6421 tctcgcagct taccacgtga gagtcatgtt agacgagact acggcgtatc aattcggtcg 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 6540

6481 gggctgtggg cggttgtggg cgactgcgcg ggactgcccg aacagacgag ggccgtaggc 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 6600

6541 gaatgtctgt tcgacactgg cagaggccct cgacgtacac agtctccaaa agtggcagta 6600

.......... .......... ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

6601 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 6660

6601 gtggctttgc gcgctctgct ttcccggagc actatgcgga taaaaatatc caattacagt 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 6720

6661 actattatta ccaaagaatc tgcagtccac cgtgaaaagc ccctttacac gcgccttggg 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 6780

6721 gataaacaaa taaaaagatt tatgtaagtt tatacatagg cgagtactct gttattggga 6780

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

6781 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 6840

6781 ctatttacga agttattata actttttcct tctcatactc ataagttgta aaggcacagc 6840

.......... .......... .......... .... Orf frame 3 Ampicillin ....

.......... .......... .......... .......... .......... ..........

6841 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 6900

6841 gggaataagg gaaaaaacgc cgtaaaacgg aaggacaaaa acgagtgggt ctttgcgacc 6900

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 6960

6901 actttcattt tctacgactt ctagtcaacc cacgtgctca cccaatgtag cttgacctag 6960

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 7020

6961 agttgtcgcc attctaggaa ctctcaaaag cggggcttct tgcaaaaggt tactactcgt 7020

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 7080

7021 gaaaatttca agacgataca ccgcgccata atagggcata actgcggccc gttctcgttg 7080

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 7140

7081 agccagcggc gtatgtgata agagtcttac tgaaccaact catgagtggt cagtgtcttt 7140

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 7200

7141 tcgtagaatg cctaccgtac tgtcattctc ttaatacgtc acgacggtat tggtactcac 7200

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 7260

7201 tattgtgacg ccggttgaat gaagactgtt gctagcctcc tggcttcctc gattggcgaa 7260

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 7320

7261 aaaacgtgtt gtacccccta gtacattgag cggaactagc aacccttggc ctcgacttac 7320

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... FspI(7380)

7321 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 7380

7321 ttcggtatgg tttgctgctc gcactgtggt gctacggaca tcgttaccgt tgttgcaacg 7380

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 7440

7381 cgtttgataa ttgaccgctt gatgaatgag atcgaagggc cgttgttaat tatctgacct 7440

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 7500

7441 acctccgcct atttcaacgt cctggtgaag acgcgagccg ggaaggccga ccgaccaaat 7500

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 7560

7501 aacgactatt tagacctcgg ccactcgcac ccagagcgcc atagtaacgt cgtgaccccg 7560

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 7620

7561 gtctaccatt cgggagggca tagcatcaat agatgtgctg cccctcagtc cgttgatacc 7620

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 7680

7621 tacttgcttt atctgtctag cgactctatc cacggagtga ctaattcgta accattgaca 7680

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 7740

7681 gtctggttca aatgagtata tatgaaatct aactaaattt tgaagtaaaa attaaatttt 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 7800

7741 cctagatcca cttctaggaa aaactattag agtactggtt ttagggaatt gcactcaaaa 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 7860

7801 gcaaggtgac tcgcagtctg gggcatcttt tctagtttcc tagaagaact ctaggaaaaa 7860

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

7861 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 7920

7861 aagacgcgca ttagacgacg aacgtttgtt tttttggtgg cgatggtcgc caccaaacaa 7920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7921 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 7980

7921 acggcctagt tctcgatggt tgagaaaaag gcttccattg accgaagtcg tctcgcgtct 7980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7981 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 8040

7981 atggtttatg acaggaagat cacatcggca tcaatccggt ggtgaagttc ttgagacatc 8040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8041 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 8100

8041 gtggcggatg tatggagcga gacgattagg acaatggtca ccgacgacgg tcaccgctat 8100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8101 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 8160

8101 tcagcacaga atggcccaac ctgagttctg ctatcaatgg cctattccgc gtcgccagcc 8160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8161 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 8220

8161 cgacttgccc cccaagcacg tgtgtcgggt cgaacctcgc ttgctggatg tggcttgact 8220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8221 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 8280

8221 ctatggatgt cgcactcgta actctttcgc ggtgcgaagg gcttccctct ttccgcctgt 8280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8281 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 8340

8281 ccataggcca ttcgccgtcc cagccttgtc ctctcgcgtg ctccctcgaa ggtccccctt 8340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8341 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 8400

8341 tgcggaccat agaaatatca ggacagccca aagcggtgga gactgaactc gcagctaaaa 8400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8401 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 8460

8401 acactacgag cagtcccccc gcctcggata cctttttgcg gtcgttgcgc cggaaaaatg 8460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8461 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 8520

8461 ccaaggaccg gaaaacgacc ggaaaacgag tgtacaagaa aggacgcaat aggggactaa 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 8580

8521 gacacctatt ggcataatgg cggaaactca ctcgactatg gcgagcggcg tcggcttgct 8580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 8640

8581 ggctcgcgtc gctcagtcac tcgctccttc gccttctcgc gggttatgcg tttggcggag 8640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg 8700

8641 aggggcgcgc aaccggctaa gtaattacgt cgacacctta cacacagtca atcccacacc 8700

.......... .......... .......... ...... pBABE_3_primer SV40_enhancer

.......... .......... .......... .......... .......... ..........

8701 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 8760

8701 tttcaggggt ccgaggggtc gtccgtcttc atacgtttcg tacgtagagt taatcagtcg 8760

.......... . SV40_enhancer SV40_promoter . .......... ..........

.......... .......... .......... .......... .......... ..........

8761 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 8820

8761 ttggtccaca cctttcaggg gtccgagggg tcgtccgtct tcatacgttt cgtacgtaga 8820

.......... . SV40_enhancer SV40_promoter . .......... ..........

.......... .......... .......... .......... .......... ..........

8821 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 8880

8821 gttaatcagt cgttggtatc agggcgggga ttgaggcggg tagggcgggg attgaggcgg 8880

.... SV40_enhancer SV40_promoter SV40_promoter ... SV40_origin

.......... .......... .......... .......... .......... ..........

8881 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 8940

8881 gtcaaggcgg gtaagaggcg gggtaccgac tgattaaaaa aaataaatac gtctccggct 8940

SV40_enhancer SV40_promoter SV40_promoter SV40_origin SV40pro_F_primer

.......... .......... .......... .......... .......... ..........

8941 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 9000

8941 ccggcggagc cggagactcg ataaggtctt catcactcct ccgaaaaaac ctccggatcc 9000

...... SV40_promoter SV40_promoter ...... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt 9060

9001 gaaaacgttt ttcgaacctg tgttctgtcc gaacgctcta tacaaactct tatggtgaaa 9060

SV40_promoter ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt 9120

9061 tagggcgcag tccctctccg tcacgcattt ttctgcgcct gagtacactt tatgaccaaa 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag 9180

9121 aatcacgcgg tctagagata ttagagcgcg ttggataaaa ggggagcttg tgaaaaattc 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca 9240

9181 ggcatctatt tgtccgaccc tgtgaagtgt actcgctttt tatgtagcag tggaccctgt 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag 9300

9241 acaacgtcta ggtacgtgca tttgagcgtt cggctgacta cggaagactt gttacctttc 9300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg 9360

9301 cgtaataacg gcattcggca ccgccagaca tggcccacgc aatgaccgcg cacttgaccc 9360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct 9420

9361 ataagcagta cagctatggc aaacataaag gtcgatgcta gtgctgttgg tcgcgctcga 9420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt 9480

9421 atttcacgac tttgcgcgtc ttccgctacc gcttccgaag tagcaataac tactggacca 9480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac 9540

9481 cctatggcca ccatgacgcc aacgctaagc actttacata ggttttcgcg tgaaacagtg 9540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca 9600

9541 gtagaagcgt tttggccgac cagcaggcga ccaactactg atacaacaac tatagggcgt 9600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg 9660

9601 tctatggacc taacttgtcg gcaccctata cccgcagcat aagcagggcg gttagaggcc 9660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt 9720

9661 agcgattaga aaagttgcgg accgtgacgg cccgcaacaa gaaaaattga agtccgccca 9720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg 9780

9721 atgttatcaa aggtcattca taagacctcc gacgtaggta ctgtgtccgt ttggactcgc 9780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta 9840

9781 tttgggacaa gtttggggcg aaatttgtag gactttggag ctgcgatcag gcggcgaaat 9840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt 9900

9841 tagtgccgcg tgttggcgga cacgtcagcc gggaactacc attttggtag ggagtgacca 9900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg 9960

9901 tagcgtacta attggcagac tacacctaga ccgcgccgta actgggtgcg ctttaggagc 9960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata 10020

9961 tgcaggtccg tgcataacac tactcgctac ggcttgcatg gctgctacta aatatgctat 10020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag 10080

10021 gccactaacc gatggcaccg ccgttgacct aaatactcac ccggggccta gaaacacttc 10080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct 10140

10081 cttggaatga agacaccaca ctgtattaac ctgtttgatg gatgtctcta aatttcgaga 10140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt 10200

10141 ttccatttat attttaaaaa ttcacatatt acacaatttg atgactaaga ttaacaaaca 10200

SV40_int .......... .......... ........ SV40_3_splice ........

.......... .......... .......... .......... .......... ..........

10201 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat 10260

10201 cataaaatct aaggttggat accttgacta cttaccctcg tcaccacctt acggaaatta 10260

SV40_3_splice ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac 10320

10261 ctccttttgg acaaaacgag tcttctttac ggtagatcac tactactccg atgacgactg 10320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct 10380

10321 agagttgtaa gatgaggagg ttttttcttc tctttccatc ttctggggtt cctgaaagga 10380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt 10440

10381 agtcttaacg attcaaaaaa ctcagtacga cacaaatcat tatcttgaga acgaacgaaa 10440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat 10500

10441 cgataaatgt ggtgtttcct ttttcgacgt gacgatatgt tcttttaata cctttttata 10500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact 10560

10501 agacattgga aatattcatc cgtattgtca atattagtat tgtatgacaa aaaagaatga 10560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc 10620

10561 ggtgtgtccg tatctcacag acgataatta ttgatacgag tttttaacac atggaaatcg 10620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10621 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat 10680

10621 aaaaattaaa catttcccca attattcctt ataaactaca tatcacggaa ctgatctcta 10680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10681 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct 10740

10681 gtattagtcg gtatggtgta aacatctcca aaatgaacga aattttttgg agggtgtgga 10740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10741 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc 10800

10741 gggggacttg gactttgtat tttacttacg ttaacaacaa caattgaaca aataacgtcg 10800

.......... .......... .......... .......... ...... SV40_PA_terminator

.......... .......... .......... .......... .......... ..........

10801 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 10860

10801 aatattacca atgtttattt cgttatcgta gtgtttaaag tgtttatttc gtaaaaaaag 10860

.......... ...... SV40_PA_terminator ...... .......... ..........

.......... .......... .......... .......... .......... ..........

10861 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa 10920

10861 tgacgtaaga tcaacaccaa acaggtttga gtagttacat agaatagtac agacctagtt 10920

........ SV40_PA_terminator EBV_rev_primer ........ ..........

.......... .......... .......... .......... .......... ..........

10921 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac 10980

10921 gacctattga gttcgattgg ttttagtagg gtttgaaggg tggggtatgg gataatggtg 10980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10981 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat 11040

10981 acggttaatg gatcaccaaa gtaaatgaga tttggacact aaggagactt aataaaagta 11040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11041 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtag..... .......... 11085

11041 aaatttcttt aacataaaca atttatacat gatgtttgaa tcatc..... .......... 11085

.......... .......... .......... .......... .......... ..........