Sequence of pLVTH

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Sequence of pLVTH

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Sequence of pLVTH : 11086 BP; 2808 A; 2684 C; 2875 G; 2719 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac 60

1 aaccttcccg attaagtgag ggtttcttct gttctatagg aactagacac ctagatggtg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 120

61 tgtgttccga tgaagggact aatcgtcttg atgtgtggtc ccggtcccca gtctataggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 180

121 gactggaaac ctaccacgat gttcgatcat ggtcaactcg gtctattcca tcttctccgg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 240

181 ttatttcctc tcttgtggtc gaacaatgtg ggacactcgg acgtacccta cctactgggc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... PmlI(291)

241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 300

241 ctctctcttc acaatctcac ctccaaactg tcggcggatc gtaaagtagt gcaccgggct 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 360

301 ctcgacgtag gcctcatgaa gttcttgacg actatagctc gaacgatgtt ccctgaaagg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 420

361 cgacccctga aaggtccctc cgcaccggac ccgccctgac ccctcaccgc tcgggagtct 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 480

421 aggacgtata ttcgtcgacg aaaaacggac atgacccaga gagaccaatc tggtctagac 480

.......... .......... .......... ......... HIV truncHIV .........

.......... .......... .......... .......... .......... ..........

481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 540

481 tcggaccctc gagagaccga ttgatccctt gggtgacgaa ttcggagtta tttcgaacgg 540

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 600

541 aactcacgaa gttcatcaca cacgggcaga caacacactg agaccattga tctctaggga 600

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 660

601 gtctgggaaa atcagtcaca ccttttagag atcgtcaccg cgggcttgtc cctgaacttt 660

......... HIV truncHIV ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 720

661 cgctttccct ttggtctcct cgagagagct gcgtcctgag ccgaacgact tcgcgcgtgc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 780

721 cgttctccgc tccccgccgc tgaccactca tgcggttttt aaaactgatc gcctccgatc 780

.......... .......... .......... .......... HIV-1 ..........

.......... .......... .......... .......... .......... ..........

781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 840

781 ttcctctctc tacccacgct ctcgcagtca taattcgccc cctcttaatc tagcgctacc 840

HIV-1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 900

841 cttttttaag ccaattccgg tccccctttc ttttttatat ttaattttgt atatcatacc 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 960

901 cgttcgtccc tcgatcttgc taagcgtcaa ttaggaccgg acaatctttg tagtcttccg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 1020

961 acatctgttt atgaccctgt cgatgttggt agggaagtct gtcctagtct tcttgaatct 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac 1080

1021 agtaatatat tatgtcatcg ttgggagata acacacgtag tttcctatct ctattttctg 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 1140

1081 tggttccttc gaaatctgtt ctatctcctt ctcgttttgt tttcattctg gtggcgtgtc 1140

.......... .......... .......... .......... .......... ..........

EagI(1145),NotI(1145) ........ .......... .......... ..........

1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga 1200

1141 gttcgccggc gactagaagt ctggacctcc tcctctatac tccctgttaa cctcttcact 1200

.......... .......... .......... .......... ORF2 ..........

.......... .......... .......... .......... .......... ..........

1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa 1260

1201 taatatattt atatttcatc atttttaact tggtaatcct catcgtgggt ggttccgttt 1260

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt t ccttgggtt 1320

1261 ctcttctcac cacgtctctc ttttttctcg tcacccttat cctcgaaaca aggaacccaa 1320

.......... .......... ORF2 .......... RRE ..........

.......... .......... .......... .......... .......... ..........

1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag 1380

1321 gaaccctcgt cgtccttcgt gatacccgcg tcgcagttac tgcgactgcc atgtccggtc 1380

.......... .......... ORF2 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca 1440

1381 tgttaataac agaccatatc acgtcgtcgt cttgttaaac gactcccgat aactccgcgt 1440

.......... .......... ORF2 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc 1500

1441 tgtcgtagac aacgttgagt gtcagacccc gtagttcgtc gaggtccgtt cttaggaccg 1500

.......... .......... ORF2 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact 1560

1501 acacctttct atggatttcc tagttgtcga ggacccctaa accccaacga gaccttttga 1560

.......... RRE ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat 1620

1561 gtaaacgtgg tgacgacacg gaaccttacg atcaacctca ttatttagag accttgtcta 1620

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat 1680

1621 aaccttagtg tgctggacct acctcaccct gtctctttaa ttgttaatgt gttcgaatta 1680

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga 1740

1681 tgtgaggaat taacttctta gcgttttggt cgttcttttc ttacttgttc ttaataacct 1740

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat 1800

1741 taatctattt acccgttcaa acaccttaac caaattgtat tgtttaaccg acaccatata 1800

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact 1860

1801 ttttaataag tattactatc atcctccgaa ccatccaaat tcttatcaaa aacgacatga 1860

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc 1920

1861 aagatatcac ttatctcaat ccgtccctat aagtggtaat agcaaagtct gggtggaggg 1920

.......... .......... ORF2 .......... .......... ORF1

.......... .......... .......... .......... .......... ..........

1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag 1980

1921 ttggggctcc cctgggctgt ccgggcttcc ttatcttctt cttccacctc tctctctgtc 1980

.......... .......... ORF2 ORF1 .......... .......... ..........

.......... .......... .......... .......... SalI(2027) ..........

1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgcgatgtcg acgataagct 2040

1981 tctgtctagg taagctaatc acttgcctag agctgccata gcgctacagc tgctattcga 2040

.......... .......... ORF2 ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag 2100

2041 aacgtttcta cctatttcaa aatttgtctc tccttagaaa cgtcgattac ctggaagatc 2100

ORF2 .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc 2160

2101 cagaactttc ctcaccctta accgaggcca cgggcagtca cccgtctcgc gtgtagcggg 2160

.......... .......... ORF1 .......... EF1a_pro ..........

.......... .......... .......... .......... .......... ..........

2161 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg 2220

2161 tgtcaggggc tcttcaaccc ccctccccag ccgttaactt ggccacggat ctcttccacc 2220

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 2280

2221 gcgccccatt tgaccctttc actacagcac atgaccgagg cggaaaaagg gctcccaccc 2280

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 2340

2281 cctcttggca tatattcacg tcatcagcgg cacttgcaag aaaaagcgtt gcccaaacgg 2340

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat 2400

2341 cggtcttgtg tccattcacg gcacacacca agggcgcccg gaccggagaa atgcccaata 2400

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggcccttgcg tgccttgaat tacttccact ggctgcagta cgtgattctt gatcccgagc 2460

2401 ccgggaacgc acggaactta atgaaggtga ccgacgtcat gcactaagaa ctagggctcg 2460

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc 2520

2461 aagcccaacc ttcacccacc ctctcaagct ccggaacgcg aattcctcgg ggaagcggag 2520

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc 2580

2521 cacgaactca actccggacc ggacccgcga ccccggcggc gcacgcttag accaccgtgg 2580

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg 2640

2581 aagcgcggac agagcgacga aagctattca gagatcggta aattttaaaa actactggac 2640

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg 2700

2641 gacgctgcga aaaaaagacc gttctatcag aacatttacg cccggttcta gacgtgtgac 2700

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt 2760

2701 cataaagcca aaaaccccgg cgcccgccgc tgccccgggc acgcagggtc gcgtgtacaa 2760

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg 2820

2761 gccgctccgc cccggacgct cgcgccggtg gctcttagcc tgcccccatc agagttcgac 2820

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa 2880

2821 cggccggacg agaccacgga ccggagcgcg gcggcacata gcggggcggg acccgccgtt 2880

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg 2940

2881 ccgaccgggc cagccgtggt caacgcactc gcctttctac cggcgaaggg ccgggacgac 2940

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac 3000

2941 gtccctcgag ttttacctcc tgcgccgcga gccctctcgc ccgcccactc agtgggtgtg 3000

.......... ........ ORF1 EF1a_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg 3060

3001 tttccttttc ccggaaaggc aggagtcggc agcgaagtac actgaggtgc ctcatggccc 3060

.......... ORF1 EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg 3120

3061 gcggcaggtc cgtggagcta atcaagagct cgaaaacctc atgcagcaga aatccaaccc 3120

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc 3180

3121 ccctccccaa aatacgctac ctcaaagggg tgtgactcac ccacctctga cttcaatccg 3180

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt 3240

3181 gtcgaaccgt gaactacatt aagaggaacc ttaaacggga aaaactcaaa cctagaacca 3240

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag 3300

3241 agtaagagtt cggagtctgt caccaagttt caaaaaaaga aggtaaagtc cacagcactc 3300

.......... EF1a_f EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gaatttcgac atttaaattt aattaatctc gacggtatcg gttaactttt aaaagaaaag 3360

3301 cttaaagctg taaatttaaa ttaattagag ctgccatagc caattgaaaa ttttcttttc 3360

.......... .......... .......... .......... .......... cPPT

.......... .......... .......... .......... .......... ..........

3361 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 3420

3361 ccccctaacc ccccatgtca cgtccccttt cttatcatct gtattatcgt tgtctgtatg 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttccgatc acgagactag 3480

3421 tttgatttct taatgttttt gtttaatgtt tttaagtttt aaaaggctag tgctctgatc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cctcgaggtt taaactacgg gatcttcgaa ggcctaagct tacgcgcgcg tcctagcgct 3540

3481 ggagctccaa atttgatgcc ctagaagctt ccggattcga atgcgcgcgc aggatcgcga 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 3600

3541 tggccagcgg tggtaccact cgttcccgct cctcgacaag tggccccacc acgggtagga 3600

.......... .......... .......... EGFP ORF2 .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 3660

3601 ccagctcgac ctgccgctgc atttgccggt gttcaagtcg cacaggccgc tcccgctccc 3660

EGFP_N .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 3720

3661 gctacggtgg atgccgttcg actgggactt caagtagacg tggtggccgt tcgacgggca 3720

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 3780

3721 cgggaccggg tgggagcact ggtgggactg gatgccgcac gtcacgaagt cggcgatggg 3780

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 3840

3781 gctggtgtac ttcgtcgtgc tgaagaagtt caggcggtac gggcttccga tgcaggtcct 3840

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 3900

3841 cgcgtggtag aagaagttcc tgctgccgtt gatgttctgg gcgcggctcc acttcaagct 3900

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 3960

3901 cccgctgtgg gaccacttgg cgtagctcga cttcccgtag ctgaagttcc tcctgccgtt 3960

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 4020

3961 gtaggacccc gtgttcgacc tcatgttgat gttgtcggtg ttgcagatat agtaccggct 4020

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 4080

4021 gttcgtcttc ttgccgtagt tccacttgaa gttctaggcg gtgttgtagc tcctgccgtc 4080

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 4140

4081 gcacgtcgag cggctggtga tggtcgtctt gtgggggtag ccgctgccgg ggcacgacga 4140

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 4200

4141 cgggctgttg gtgatggact cgtgggtcag gcgggactcg tttctggggt tgctcttcgc 4200

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 4260

4201 gctagtgtac caggacgacc tcaagcactg gcggcggccc tagtgagagc cgtacctgct 4260

.......... EGFP_C EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gctgtacaag tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa 4320

4261 cgacatgttc aggcctgagt ctagagctat cgatcatcga tcgatcgatc agctcgagtt 4320

EGFP ORF2 .......... .......... .......... ..........

SpeI(4330) NdeI(4337) .......... .......... .......... ..........

4321 gcttcgggga ctagtcatat gataatcaac ctctggatta caaaatttgt gaaagattga 4380

4321 cgaagcccct gatcagtata ctattagttg gagacctaat gttttaaaca ctttctaact 4380

.......... .......... .......... .......... WPRE ..........

.......... .......... .......... .......... .......... ..........

4381 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 4440

4381 gaccataaga attgatacaa cgaggaaaat gcgatacacc tatgcgacga aattacggaa 4440

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 4500

4441 acatagtacg ataacgaagg gcataccgaa agtaaaagag gaggaacata tttaggacca 4500

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 4560

4501 acgacagaga aatactcctc aacaccgggc aacagtccgt tgcaccgcac cacacgtgac 4560

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 4620

4561 acaaacgact gcgttggggg tgaccaaccc cgtaacggtg gtggacagtc gaggaaaggc 4620

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 4680

4621 cctgaaagcg aaagggggag ggataacggt gccgccttga gtagcggcgg acggaacggg 4680

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 4740

4681 cgacgacctg tccccgagcc gacaacccgt gactgttaag gcaccacaac agccccttcg 4740

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 4800

4741 actgcaggaa aggtaccgac gagcggacac aacggtggac ctaagacgcg ccctgcagga 4800

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 4860

4801 agacgatgca gggaagccgg gagttaggtc gcctggaagg aagggcgccg gacgacggcc 4860

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 4920

4861 gagacgccgg agaaggcgca gaagcggaag cgggagtctg ctcagcctag agggaaaccc 4920

.......... .......... WPRE .......... .......... ..........

.......... .......... MscI(4946) .......... .......... ..........

4921 ccgcctcccc gcatcggtac gtatggccag gtacctttaa gaccaatgac ttacaaggca 4980

4921 ggcggagggg cgtagccatg cataccggtc catggaaatt ctggttactg aatgttccgt 4980

WPRE .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 5040

4981 cgacatctag aatcggtgaa aaattttctt ttcccccctg accttcccga ttaagtgagg 5040

.......... .......... .......... cPPT U3PPT .......... ..........

.......... .......... ......... BamHI(5078) ......... ..........

5041 caacgaagac aagatgggat caattcacca tgctagtgga tccccctcga gtttaccact 5100

5041 gttgcttctg ttctacccta gttaagtggt acgatcacct agggggagct caaatggtga 5100

.......... .......... .......... .......... .......... TRE

.......... .......... .......... .......... .......... ..........

5101 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga 5160

5101 gggatagtca ctatctcttt tcactttcag ctcaaatggt gagggatagt cactatctct 5160

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt 5220

5161 tttcactttc agctcaaatg gtgagggata gtcactatct cttttcactt tcagctcaaa 5220

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga 5280

5221 tggtgaggga tagtcactat ctcttttcac tttcagctca aatggtgagg gatagtcact 5280

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc 5340

5281 atctcttttc actttcagct caaatggtga gggatagtca ctatctcttt tcactttcag 5340

.......... .......... TRE .......... .......... ..........

.......... .......... .......... .......... ......... EcoRI(5393)

5341 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcggggctgc aggaattcga 5400

5341 ctcaaatggt gagggatagt cactatctct tttcactttc agccccgacg tccttaagct 5400

.......... TRE .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca 5460

5401 tgcgactgca gtagttgggc gaggttcctt agcgcccggg tcacagtgat ccgcccttgt 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg gacaggggag tggcgccctg 5520

5461 gggtcgcgcg cacgcgggac cgtccttcta ccgacactcc ctgtcccctc accgcgggac 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga 5580

5521 gttataaacg tacagcgata cacaagaccc tttagtggta tttgcacttt acagaaacct 5580

.......... .......... H1_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tttgggaatc ttataagttc tgtatgagac cacagatctc caagcttatc gataccgtcg 5640

5581 aaacccttag aatattcaag acatactctg gtgtctagag gttcgaatag ctatggcagc 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 catgggaata acttcgtata gcatacatta tacgaagtta tgctgctttt tgcttgtact 5700

5641 gtacccttat tgaagcatat cgtatgtaat atgcttcaat acgacgaaaa acgaacatga 5700

.......... .......... loxP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 5760

5701 cccagagaga ccaatctggt ctagactcgg accctcgaga gaccgattga tcccttgggt 5760

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 5820

5761 gacgaattcg gagttatttc gaacggaact cacgaagttc atcacacacg ggcagacaac 5820

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 5880

5821 acactgagac cattgatctc tagggagtct gggaaaatca gtcacacctt ttagagatcg 5880

.......... ......... HIV truncHIV ......... .......... ..........

XbaI(5885) .......... .......... .......... .......... ..........

5881 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac 5940

5881 tcgtagatct taattaaggc acataagata tcacagtgga tttagcatac acatactatg 5940

.......... .......... .......... Sp6_pri .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg 6000

5941 tattccaata cataattaac atcggcgcaa gattgctgtt atacatgttc ggattaacac 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg 6060

6001 atcgtagacc gaatgacttc gtctgggata gtagagagag catttgacgg cagtctcagc 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca 6120

6061 caaaccaacc tgcttggaag actcaaagac cattgcggca gggcgtgggc ctttaccagt 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg 6180

6121 cgcttggtta gtcgtcccag tagcgatcgg tctaggagat gcggcctgcg tagcaccggc 6180

.......... .......... .......... .......... .......... tet

.......... .......... .......... .......... .......... ..........

6181 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg 6240

6181 cgtagtggcc gcggtgtcca cgccaacgac cgcggatata gcggctgtag t ggctacccc 6240

.......... .......... tet ........ pBRrevBam_pri ........

.......... .......... .......... .......... .......... ..........

6241 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag 6300

6241 ttctagcccg agcggtgaag cccgagtact cgcgaacaaa gccgcaccca taccaccgtc 6300

.......... .......... tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg 6360

6301 cggggcaccg gccccctgac aacccgcggt agaggaacgt acgtggtaag gaacgccgcc 6360

.......... .......... tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg 6420

6361 gccacgagtt gccggagttg gatgatgacc cgacgaagga ttacgtcctc agcgtattcc 6420

.......... .......... tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag 6480

6421 ctctcgcagc ttaccacgtg agagtcatgt tagacgagac tacggcgtat caattcggtc 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc 6540

6481 ggggctgtgg gcggttgtgg gcgactgcgc gggactgccc gaacagacga gggccgtagg 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca 6600

6541 cgaatgtctg ttcgacactg gcagaggccc tcgacgtaca cagtctccaa aagtggcagt 6600

.......... .......... .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc 6660

6601 agtggctttg cgcgctctgc tttcccggag cactatgcgg ataaaaatat ccaattacag 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 6720

6661 tactattatt accaaagaat ctgcagtcca ccgtgaaaag cccctttaca cgcgccttgg 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 6780

6721 ggataaacaa ataaaaagat ttatgtaagt ttatacatag gcgagtactc tgttattggg 6780

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

6781 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 6840

6781 actatttacg aagttattat aactttttcc ttctcatact cataagttgt aaaggcacag 6840

.......... .......... .......... ....... ORF1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

6841 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 6900

6841 cgggaataag ggaaaaaacg ccgtaaaacg gaaggacaaa aacgagtggg tctttgcgac 6900

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 6960

6901 cactttcatt ttctacgact tctagtcaac ccacgtgctc acccaatgta gcttgaccta 6960

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 7020

6961 gagttgtcgc cattctagga actctcaaaa gcggggcttc ttgcaaaagg ttactactcg 7020

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 7080

7021 tgaaaatttc aagacgatac accgcgccat aatagggcat aactgcggcc cgttctcgtt 7080

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 7140

7081 gagccagcgg cgtatgtgat aagagtctta ctgaaccaac tcatgagtgg tcagtgtctt 7140

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 7200

7141 ttcgtagaat gcctaccgta ctgtcattct cttaatacgt cacgacggta ttggtactca 7200

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 7260

7201 ctattgtgac gccggttgaa tgaagactgt tgctagcctc ctggcttcct cgattggcga 7260

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 7320

7261 aaaaacgtgt tgtaccccct agtacattga gcggaactag caacccttgg cctcgactta 7320

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 7380

7321 cttcggtatg gtttgctgct cgcactgtgg tgctacggac atcgttaccg ttgttgcaac 7380

.......... ....... ORF1 Ampicillin ....... .......... ..........

FspI(7381) .......... .......... .......... .......... ..........

7381 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 7440

7381 gcgtttgata attgaccgct tgatgaatga gatcgaaggg ccgttgttaa ttatctgacc 7440

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 7500

7441 tacctccgcc tatttcaacg tcctggtgaa gacgcgagcc gggaaggccg accgaccaaa 7500

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 7560

7501 taacgactat ttagacctcg gccactcgca cccagagcgc catagtaacg tcgtgacccc 7560

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 7620

7561 ggtctaccat tcgggagggc atagcatcaa tagatgtgct gcccctcagt ccgttgatac 7620

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 7680

7621 ctacttgctt tatctgtcta gcgactctat ccacggagtg actaattcgt aaccattgac 7680

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 7740

7681 agtctggttc aaatgagtat atatgaaatc taactaaatt ttgaagtaaa aattaaattt 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 7800

7741 tcctagatcc acttctagga aaaactatta gagtactggt tttagggaat tgcactcaaa 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 7860

7801 agcaaggtga ctcgcagtct ggggcatctt ttctagtttc ctagaagaac tctaggaaaa 7860

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

7861 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 7920

7861 aaagacgcgc attagacgac gaacgtttgt ttttttggtg gcgatggtcg ccaccaaaca 7920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7921 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 7980

7921 aacggcctag ttctcgatgg ttgagaaaaa ggcttccatt gaccgaagtc gtctcgcgtc 7980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7981 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 8040

7981 tatggtttat gacaggaaga tcacatcggc atcaatccgg tggtgaagtt cttgagacat 8040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8041 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 8100

8041 cgtggcggat gtatggagcg agacgattag gacaatggtc accgacgacg gtcaccgcta 8100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8101 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 8160

8101 ttcagcacag aatggcccaa cctgagttct gctatcaatg gcctattccg cgtcgccagc 8160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8161 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 8220

8161 ccgacttgcc ccccaagcac gtgtgtcggg tcgaacctcg cttgctggat gtggcttgac 8220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8221 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac 8280

8221 tctatggatg tcgcactcgt aactctttcg cggtgcgaag ggcttccctc tttccgcctg 8280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8281 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 8340

8281 tccataggcc attcgccgtc ccagccttgt cctctcgcgt gctccctcga aggtccccct 8340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8341 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 8400

8341 ttgcggacca tagaaatatc aggacagccc aaagcggtgg agactgaact cgcagctaaa 8400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8401 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 8460

8401 aacactacga gcagtccccc cgcctcggat acctttttgc ggtcgttgcg ccggaaaaat 8460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8461 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 8520

8461 gccaaggacc ggaaaacgac cggaaaacga gtgtacaaga aaggacgcaa taggggacta 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 8580

8521 agacacctat tggcataatg gcggaaactc actcgactat ggcgagcggc gtcggcttgc 8580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 8640

8581 tggctcgcgt cgctcagtca ctcgctcctt cgccttctcg cgggttatgc gtttggcgga 8640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg 8700

8641 gaggggcgcg caaccggcta agtaattacg tcgacacctt acacacagtc aatcccacac 8700

.......... .......... .......... ......... pBABE_3_pri SV40_enh

.......... .......... .......... .......... .......... ..........

8701 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 8760

8701 ctttcagggg tccgaggggt cgtccgtctt catacgtttc gtacgtagag ttaatcagtc 8760

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 8820

8761 gttggtccac acctttcagg ggtccgaggg gtcgtccgtc ttcatacgtt tcgtacgtag 8820

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 8880

8821 agttaatcag tcgttggtat cagggcgggg attgaggcgg gtagggcggg gattgaggcg 8880

.......... . SV40_enh SV40_pro SV40_pro SV40_origin . ..........

.......... .......... .......... .......... .......... ..........

8881 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 8940

8881 ggtcaaggcg ggtaagaggc ggggtaccga ctgattaaaa aaaataaata cgtctccggc 8940

SV40_enh SV40_pro SV40_pro SV40_origin ........ SV40pro_F_pri

.......... .......... .......... .......... .......... ..........

8941 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 9000

8941 tccggcggag ccggagactc gataaggtct tcatcactcc tccgaaaaaa cctccggatc 9000

..... SV40_pro SV40_origin SV40_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt 9060

9001 cgaaaacgtt tttcgaacct gtgttctgtc cgaacgctct atacaaactc ttatggtgaa 9060

SV40_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt 9120

9061 atagggcgca gtccctctcc gtcacgcatt tttctgcgcc tgagtacact ttatgaccaa 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa 9180

9121 aaatcacgcg gtctagagat attagagcgc gttggataaa aggggagctt gtgaaaaatt 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac 9240

9181 cggcatctat ttgtccgacc ctgtgaagtg tactcgcttt ttatgtagca gtggaccctg 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa 9300

9241 tacaacgtct aggtacgtgc atttgagcgt tcggctgact acggaagact tgttaccttt 9300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg 9360

9301 ccgtaataac ggcattcggc accgccagac atggcccacg caatgaccgc gcacttgacc 9360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc 9420

9361 cataagcagt acagctatgg caaacataaa ggtcgatgct agtgctgttg gtcgcgctcg 9420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg 9480

9421 aatttcacga ctttgcgcgt cttccgctac cgcttccgaa gtagcaataa ctactggacc 9480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca 9540

9481 acctatggcc accatgacgc caacgctaag cactttacat aggttttcgc gtgaaacagt 9540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc 9600

9541 ggtagaagcg ttttggccga ccagcaggcg accaactact gatacaacaa ctatagggcg 9600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg 9660

9601 ttctatggac ctaacttgtc ggcaccctat acccgcagca taagcagggc ggttagaggc 9660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg 9720

9661 cagcgattag aaaagttgcg gaccgtgacg gcccgcaaca agaaaaattg aagtccgccc 9720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc 9780

9721 aatgttatca aaggtcattc ataagacctc cgacgtaggt actgtgtccg tttggactcg 9780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt 9840

9781 ctttgggaca agtttggggc gaaatttgta ggactttgga gctgcgatca ggcggcgaaa 9840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg 9900

9841 ttagtgccgc gtgttggcgg acacgtcagc cgggaactac cattttggta gggagtgacc 9900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc 9960

9901 atagcgtact aattggcaga ctacacctag accgcgccgt aactgggtgc gctttaggag 9960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat 10020

9961 ctgcaggtcc gtgcataaca ctactcgcta cggcttgcat ggctgctact aaatatgcta 10020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa 10080

10021 tgccactaac cgatggcacc gccgttgacc taaatactca cccggggcct agaaacactt 10080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc 10140

10081 ccttggaatg aagacaccac actgtattaa cctgtttgat ggatgtctct aaatttcgag 10140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg 10200

10141 attccattta tattttaaaa attcacatat tacacaattt gatgactaag attaacaaac 10200

.......... .......... SV40_int ........ SV40_3_splice ........

.......... .......... .......... .......... .......... ..........

10201 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa 10260

10201 acataaaatc taaggttgga taccttgact acttaccctc gtcaccacct tacggaaatt 10260

SV40_3_splice ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga 10320

10261 actccttttg gacaaaacga gtcttcttta cggtagatca ctactactcc gatgacgact 10320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc 10380

10321 gagagttgta agatgaggag gttttttctt ctctttccat cttctggggt tcctgaaagg 10380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt 10440

10381 aagtcttaac gattcaaaaa actcagtacg acacaaatca ttatcttgag aacgaacgaa 10440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata 10500

10441 acgataaatg tggtgtttcc tttttcgacg tgacgatatg ttcttttaat acctttttat 10500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac 10560

10501 aagacattgg aaatattcat ccgtattgtc aatattagta ttgtatgaca aaaaagaatg 10560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag 10620

10561 aggtgtgtcc gtatctcaca gacgataatt attgatacga gtttttaaca catggaaatc 10620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10621 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga 10680

10621 gaaaaattaa acatttcccc aattattcct tataaactac atatcacgga actgatctct 10680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10681 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc 10740

10681 agtattagtc ggtatggtgt aaacatctcc aaaatgaacg aaattttttg gagggtgtgg 10740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10741 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag 10800

10741 agggggactt ggactttgta ttttacttac gttaacaaca acaattgaac aaataacgtc 10800

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

10801 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 10860

10801 gaatattacc aatgtttatt tcgttatcgt agtgtttaaa gtgtttattt cgtaaaaaaa 10860

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10861 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca 10920

10861 gtgacgtaag atcaacacca aacaggtttg agtagttaca tagaatagta cagacctagt 10920

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

10921 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca 10980

10921 tgacctattg agttcgattg gttttagtag ggtttgaagg gtggggtatg ggataatggt 10980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10981 ctgccaatta cctagtggtt tcatttactc taaacctgtg attcctctga attattttca 11040

10981 gacggttaat ggatcaccaa agtaaatgag atttggacac taaggagact taataaaagt 11040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11041 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtag.... .......... 11086

11041 aaaatttctt taacataaac aatttataca tgatgtttga atcatc.... .......... 11086

.......... .......... .......... .......... .......... ..........