Download the plasmid map
Use BVTech Plasmid to view and edit this plasmid map.
Dowload BVTech Plasmid
If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.
BVTech Plasmid is DNA sequence analysis and plasmid drawing software for Windows PCs.
You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your
DNA sequencing data, align sequences, and much more.
Free to try, $39.98 to buy
Payments will be processed by PayPal. PayPal is the safer, easier way to pay.
View the sequence with features on line
Vector backbone: pLionII
Backbone manufacturer: Nolan Lab
Type of vector: Lentiviral
Backbone size (bp): 7002
5' Sequencing primer: n/a
3' Sequencing primer: M13 reverse primer
Bacteria resistance: Ampicillin
High or low copy: Don't Know
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Garry Nolan
Comments: This is a transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB.