h]Ch]C+>pLionII plasmid sequence 7002 bp AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGG AAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCC GGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTG CATGCCtgcagtgcgaagttctcggcccggattccgagacctcacaggtaaaaaagattacacagatactcgacagggtt caatctcaaatatttattgtatctgtgggagcctcaagggagaactcaaaagtcctcaacaaagagactcctcgaagttt cacaaagcactggttaaggccttcagggttccaatactcatcccagtccacccttttttcttttttaggagGGCCCCCCC TCGAGGTCGACGGTATCGATCAGGCGGGGAGGCGGCCCAAAGGGAGATCCGACTCGTCTGAGGGCGAAGGCGAAGACGCG GAAGAGGCCGCAGAGCCGGCAGCAGGCCGCGGGAAGGAAGGTCCGCTGGATTGAGGGCCGAAGGGACGTAGCAGAAGGAC GTCCCGCGCAGAATCCAGGTGGCAACACAGGCGAGCAGCCAaGGAAAGGACGatgatTTCCCCGACAACACCACGGAATT GTCAGTGCCCAACAGCCGAGCCCCTGTCCAGCAGCGGGCAAGGCAGGCGGCGATGAGTTCCGCCGTGGCAATAGGGAGGG GGAAAGCGAAAGTCCCGGAAAGGAGCTGACAGGTGGTGGCAATGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACAGTG CACACCACGCCACGTTGCCTGACAACGGGCCACAACTCCTCATAAAGAGACAGCAACCAGGATTTATACAAGGAGGAGAA AATGAAAGCCATACGGGAAGCAATAGCATGATACAAAGGCATTAAAGCAGCGTATCCACATAGCGTAAAAGGAGCAACAT AGTTAAGAATACCAGTCAATCTTTCACAAATTTTGTAATCCAGAGGTTGATTatcgatAAGCTTGATgaaggctttcttc tttcttcttcttctttgtcttttccttttaccttacaatacatactttattagtttgatttgattcgaaatggattcata tgacataccttcctcaaagggaagaactcagcacagtatctcccttataaaataagtctgagatacttcatcattcctcc tctttttcagacatgccacattgcctaccatttctcctcaattccatttgtggttgtatttcttctccttctacttcagg cattgcaattactgtgtatcctagtatcttgtggatacaatttcttatacaatcaaccaatgtaggtaaacataaaatca ataataacactcctaatcctattcccaagatacctcccaatagtccctttaaatattgtggaatatttcctatccatcct acccaatcttcccacttttgtaattgttgtatccctttcttcccttgtacattattttgttctatgtccattattatttc ataaaacttttgttgtaaatcttttgtttggttataccattcccccaaagttatatttccatgattccatattgtttgat ttatagtcatattataccttgtccacaactcaggagggattttgcagaaaaattgattttgattacatcctaattcttgc atagcgaaagctgtatacaaaaatttttccatagcttctacttttaatcctattactagCTTCTATTAGGGCGCACTCGA GCGGCCGCCACTGTGCTGGATATCTGCAGAATTCCACCACACTGGACTAGTGGATCCGAGCTCGGTACCAAGCTTAAGTT TAAACGCTAGCCAGCTTGGGTCTCCCTATAGTGAGTCGTATTAATTTCGATAAGCCAGTAAGCAGTGGGTTCTCTAGTTA GCCAGAGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGA CATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCG TGAGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGA TGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCA ATAGGGGGCGTACTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAAATAGTCCACCCATTGACG TCAATGGAAAGTCCCTATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGCGGTC AGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGCGGAACTCCATATATGGGCTATGAACTAATGACCCCGTAATTGAT TACTATTAATAACTAGTCAATAATCAATGTCAACGCGTATATCTGGCCCGTACATCGCGGTACCCAATTCGCCCTATAGT GAGTCGTATTACGCGCGccgtcatatttaaaagtcctgctaccgcaaagacttttaatgtcacaattactccttccatct tttgatttttaaaatttggtccattatctgtttgtaattcagtaacattatgagcactcaacaattgtaagacagcttta actgtacagtcagcagtttcttgagaaattatttgtgcccatatatatcctgattccacatgtatacaattgccatatca atttctttgctagatccaaatttttctcttctttcttgtaaatcgcaaataaccaaccttagttgatctaaagtctctgg tatatcaccaggttctcgtcctgtagatacattagccattctaatggcccatctgaaattcccttctccaaattttttac tcttcccccctactcctacagcaacattactacatctcttaatggccattttccaatctcgcccctgtccattccccatg ttgctgtagaatctctcctaccttgttgactgtccctcggcgaatctcctggcttgaaggtccgcgaagagtcaccagat gtaatttatctgggcctttaaacaatgacttgattatgagctcgagtccgcttcactagagatactcactgttagcagcg tctgctactgcttccctatttaggtcagcaggagttctgcttaacagctttctattgctctagcttcacttcctcaatca ctctcaatcaagtccctgttcgggcgccaactgcgaagttctcggcccggattccgagacctcacaggtaaaaaagatta cacagatactcgacagggttcaatctcaaatatttattgtatctgtgggagcctcaagggagaactcaaaagtcctcaac aaaGAGACTCCTCGAAGTTTCACAGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAAC GGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTG GAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGCCAAAACCGCATCACCATGGTAATAG CGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCGTAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCAT TTACCGTCATTGACGTCAATAGGGGGCGGACTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAA ATACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGGC GGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGCGGAACTCCATATATGGGCTATGAACT AATGACCCCGTAATTGATTACTATTAATAACTAGTCAATAATCAATGCCAACATGGCGGTCATATTGGACATGAGCCAAT ATAAATGTACATATTATGATATAGATACAACGTATGCAATGGCCAATAGCCAATATTGATTTATGCTATATAACCAATGA ATAATATGGCTAATGGCCAATATTGAAGATCTGTCGAGCCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCC GCTTACCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACC CCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAG TCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATA AGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATAT TAACGTTTACAATTTTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCA ACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTG CATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTA ATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAG TATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAA CGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATC CCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCA CAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC AACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCT TGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAA CGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTC TCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAA CTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAC TCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCAT GACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATC CTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAG CTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTT AGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTG GCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGT TCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCAC GCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAG GGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCA GGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACAT GTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCC GAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGZpLionII "Times New Roman"h]CCZ lac_promoter"Times New Romanh]CCZM13_pUC_rev_primer"Times New Romanh]CCɆZM13_reverse_primer"Times New Romanh]CCɆZORF1"Times New Romanh]CZU3PPT"Times New Romanh]CCZU3PPT"Times New Roman)h]CCZpBluescriptKS_primer"Times New Romanh]CZ=WPRE"Times New Romanh]CCZEnv_FIV"Times New Romanh]CCZ T7_promoter"Times New Romanh]CZ CMV_immearly_promoter"Times New Romanh]CCɆZ CMV_fwd_primer"Times New Romanh]CCZ  CAG_enhancer"Times New Romanh]CCZI [  T7_promoter"Times New Romanh]CZ CMV_immearly_promoter"Times New Romanh]CCɆZ CMV_fwd_primer"Times New Romanh]CCZ CAG_enhancer"Times New Romanh]CCZWm pGEX_3_primer"Times New Romanh]CCɆZ ( AmpR_promoter"Times New Romanh]CZR Orf frame 3"Times New Roman(h]CCZR Ampicillin"Times New Romanh]CCZI pBR322_origin"Times New Roman h]CPvuII"Times New Roman7&ZwhwTw@\1@ h]CStuI"Times New Roman ZweRCR h]CApaI(475), SalI(486)"Times New Roman'Z|j\>-\ h]CSacII"Times New RomanNZvpiCw h]CBstBI"Times New Roman Z<M^^ h]CVNotI(1842), EagI(1842), EcoRV(1861), EcoRI(1869), BamHI(1892), AflII(1913), NheI(1926)"Times New RomanMZZmnoq= h]CNarI"Times New Roman8 (Z@Tgrgv h]CBglII"Times New Roman Z ++: h]CFspI"Times New Roman Zv8v h]Ctest"Times New Roman#Zo2^ h]Ctest"Times New RomanZB#F^ h]Ctest"Times New Roman2WZ3&&^