Sequence of pLionII

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Sequence of pLionII

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Sequence of pLionII : 7002 BP; 1845 A; 1712 C; 1568 G; 1877 T; 0 other;

.......... .......... .......... .......... .......... PvuII(55)

1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60

1 tcgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg ctaagtaatt acgtcgaccg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120

61 tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt gcgttaatta cactcaatcg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180

121 agtgagtaat ccgtggggtc cgaaatgtga aatacgaagg ccgagcatac aacacacctt 180

.......... .......... ......... lac_promoter ......... ..........

.......... .......... .......... .......... .......... ..........

181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 240

181 aacactcgcc tattgttaaa gtgtgtcctt tgtcgatact ggtactaatg cggttcgaac 240

...... M13_pUC_rev_primer .. M13_reverse_primer ...... ..........

.......... .......... .......... .......... .......... ..........

241 catgcctgca gtgcgaagtt ctcggcccgg attccgagac ctcacaggta aaaaagatta 300

241 gtacggacgt cacgcttcaa gagccgggcc taaggctctg gagtgtccat tttttctaat 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cacagatact cgacagggtt caatctcaaa tatttattgt atctgtggga gcctcaaggg 360

301 gtgtctatga gctgtcccaa gttagagttt ataaataaca tagacaccct cggagttccc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... StuI(419)

361 agaactcaaa agtcctcaac aaagagactc ctcgaagttt cacaaagcac tggttaaggc 420

361 tcttgagttt tcaggagttg tttctctgag gagcttcaaa gtgtttcgtg accaattccg 420

.......... .......... .......... .......... ORF1 ..........

.......... .......... .......... .......... .......... ..........

421 cttcagggtt ccaatactca tcccagtcca cccttttttc ttttttagga gggccccccc 480

421 gaagtcccaa ggttatgagt agggtcaggt gggaaaaaag aaaaaatcct cccggggggg 480

.......... .......... ORF1 U3PPT U3PPT .......... ..........

SalI(486) .......... .......... .......... .......... ..........

481 tcgaggtcga cggtatcgat caggcgggga ggcggcccaa agggagatcc gactcgtctg 540

481 agctccagct gccatagcta gtccgcccct ccgccgggtt tccctctagg ctgagcagac 540

pBluescriptKS_primer ORF1 .......... WPRE ..........

.......... .......... .......... .......... SacII(590) ..........

541 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag 600

541 tcccgcttcc gcttctgcgc cttctccggc gtctcggccg tcgtccggcg cccttccttc 600

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt 660

601 caggcgacct aactcccggc ttccctgcat cgtcttcctg cagggcgcgt cttaggtcca 660

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ggcaacacag gcgagcagcc aaggaaagga cgatgatttc cccgacaaca ccacggaatt 720

661 ccgttgtgtc cgctcgtcgg ttcctttcct gctactaaag gggctgttgt ggtgccttaa 720

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc 780

721 cagtcacggg ttgtcggctc ggggacaggt cgtcgcccgt tccgtccgcc gctactcaag 780

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc 840

781 gcggcaccgt tatccctccc cctttcgctt tcagggcctt tcctcgactg tccaccaccg 840

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct 900

841 ttacggggtt ggtcaccccc aacgcagtcg tttgtgtcac gtgtggtgcg gtgcaacgga 900

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa 960

901 ctgttgcccg gtgttgagga gtatttctct gtcgttggtc ctaaatatgt tcctcctctt 960

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca 1020

961 ttactttcgg tatgcccttc gttatcgtac tatgtttccg taatttcgtc gcataggtgt 1020

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc 1080

1021 atcgcatttt cctcgttgta tcaattctta tggtcagtta gaaagtgttt aaaacattag 1080

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cagaggttga ttatcgataa gcttgatgaa ggctttcttc tttcttcttc ttctttgtct 1140

1081 gtctccaact aatagctatt cgaactactt ccgaaagaag aaagaagaag aagaaacaga 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... BstBI(1185) .........

1141 tttcctttta ccttacaata catactttat tagtttgatt tgattcgaaa tggattcata 1200

1141 aaaggaaaat ggaatgttat gtatgaaata atcaaactaa actaagcttt acctaagtat 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgacatacct tcctcaaagg gaagaactca gcacagtatc tcccttataa aataagtctg 1260

1201 actgtatgga aggagtttcc cttcttgagt cgtgtcatag agggaatatt ttattcagac 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 agatacttca tcattcctcc tctttttcag acatgccaca ttgcctacca tttctcctca 1320

1261 tctatgaagt agtaaggagg agaaaaagtc tgtacggtgt aacggatggt aaagaggagt 1320

.......... .......... .......... Env_FIV .......... ..........

.......... .......... .......... .......... .......... ..........

1321 attccatttg tggttgtatt tcttctcctt ctacttcagg cattgcaatt actgtgtatc 1380

1321 taaggtaaac accaacataa agaagaggaa gatgaagtcc gtaacgttaa tgacacatag 1380

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ctagtatctt gtggatacaa tttcttatac aatcaaccaa tgtaggtaaa cataaaatca 1440

1381 gatcatagaa cacctatgtt aaagaatatg ttagttggtt acatccattt gtattttagt 1440

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ataataacac tcctaatcct attcccaaga tacctcccaa tagtcccttt aaatattgtg 1500

1441 tattattgtg aggattagga taagggttct atggagggtt atcagggaaa tttataacac 1500

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gaatatttcc tatccatcct acccaatctt cccacttttg taattgttgt atccctttct 1560

1501 cttataaagg ataggtagga tgggttagaa gggtgaaaac attaacaaca tagggaaaga 1560

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tcccttgtac attattttgt tctatgtcca ttattatttc ataaaacttt tgttgtaaat 1620

1561 agggaacatg taataaaaca agatacaggt aataataaag tattttgaaa acaacattta 1620

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cttttgtttg gttataccat tcccccaaag ttatatttcc atgattccat attgtttgat 1680

1621 gaaaacaaac caatatggta agggggtttc aatataaagg tactaaggta taacaaacta 1680

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttatagtcat attatacctt gtccacaact caggagggat tttgcagaaa aattgatttt 1740

1681 aatatcagta taatatggaa caggtgttga gtcctcccta aaacgtcttt ttaactaaaa 1740

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gattacatcc taattcttgc atagcgaaag ctgtatacaa aaatttttcc atagcttcta 1800

1741 ctaatgtagg attaagaacg tatcgctttc gacatatgtt tttaaaaagg tatcgaagat 1800

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cttttaatcc tattactagc ttctattagg gcgcactcga gcggccgcca ctgtgctgga 1860

1801 gaaaattagg ataatgatcg aagataatcc cgcgtgagct cgccggcggt gacacgacct 1860

Env_FIV .......... .......... .......... .......... ..........

EcoRV(1861) EcoRI(1869) ..... BamHI(1892) ......... ..........

1861 tatctgcaga attccaccac actggactag tggatccgag ctcggtacca agcttaagtt 1920

1861 atagacgtct taaggtggtg tgacctgatc acctaggctc gagccatggt tcgaattcaa 1920

.......... .......... .......... .......... .......... ..........

NheI(1926) .......... .......... .......... .......... ..........

1921 taaacgctag ccagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta 1980

1921 atttgcgatc ggtcgaaccc agagggatat cactcagcat aattaaagct attcggtcat 1980

.......... .......... ......... T7_promoter ......... ..........

.......... .......... .......... .......... .......... ..........

1981 agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg 2040

1981 tcgtcaccca agagatcaat cggtctctcg agacgaatat atctggaggg tggcatgtgc 2040

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg 2100

2041 ggatggcggg taaacgcagt taccccgcct caacaatgct gtaaaacctt tcagggcaac 2100

CMV_fwd_primer CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg 2160

2101 taaaaccacg gttttgtttg agggtaactg cagttacccc acctctgaac ctttaggggc 2160

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat 2220

2161 actcagtttg gcgataggtg cgggtaacta catgacggtt ttggcgtagt ggtaccatta 2220

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg 2280

2221 tcgctactga ttatgcatct acatgacggt tcatcctttc agggtattcc agtacatgac 2280

.......... ... CMV_immearly_promoter CAG_enhancer ... ..........

.......... .......... .......... .......... .......... ..........

2281 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat 2340

2281 ccgtattacg gtccgcccgg taaatggcag taactgcagt tatcccccgc atgaaccgta 2340

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

2341 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatagtcca cccattgacg 2400

2341 tactatgtga actacatgac ggttcacccg tcaaatggca tttatcaggt gggtaactgc 2400

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

2401 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg 2460

2401 agttaccttt cagggataac cgcaatgata cccttgtatg cagtaataac tgcagttacc 2460

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

2461 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa 2520

2461 cgcccccagc aacccgccag tcggtccgcc cggtaaatgg cattcaatac attgcgcctt 2520

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

2521 ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa 2580

2521 gaggtatata cccgatactt gattactggg gcattaacta atgataatta ttgatcagtt 2580

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 taatcaatgt caacgcgtat atctggcccg tacatcgcgg tacccaattc gccctatagt 2640

2581 attagttaca gttgcgcata tagaccgggc atgtagcgcc atgggttaag cgggatatca 2640

CMV_immearly_promoter ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gagtcgtatt acgcgcgccg tcatatttaa aagtcctgct accgcaaaga cttttaatgt 2700

2641 ctcagcataa tgcgcgcggc agtataaatt ttcaggacga tggcgtttct gaaaattaca 2700

T7_promoter ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cacaattact ccttccatct tttgattttt aaaatttggt ccattatctg tttgtaattc 2760

2701 gtgttaatga ggaaggtaga aaactaaaaa ttttaaacca ggtaatagac aaacattaag 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agtaacatta tgagcactca acaattgtaa gacagcttta actgtacagt cagcagtttc 2820

2761 tcattgtaat actcgtgagt tgttaacatt ctgtcgaaat tgacatgtca gtcgtcaaag 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttgagaaatt atttgtgccc atatatatcc tgattccaca tgtatacaat tgccatatca 2880

2821 aactctttaa taaacacggg tatatatagg actaaggtgt acatatgtta acggtatagt 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atttctttgc tagatccaaa tttttctctt ctttcttgta aatcgcaaat aaccaacctt 2940

2881 taaagaaacg atctaggttt aaaaagagaa gaaagaacat ttagcgttta ttggttggaa 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 agttgatcta aagtctctgg tatatcacca ggttctcgtc ctgtagatac attagccatt 3000

2941 tcaactagat ttcagagacc atatagtggt ccaagagcag gacatctatg taatcggtaa 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctaatggccc atctgaaatt cccttctcca aattttttac tcttcccccc tactcctaca 3060

3001 gattaccggg tagactttaa gggaagaggt ttaaaaaatg agaagggggg atgaggatgt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gcaacattac tacatctctt aatggccatt ttccaatctc gcccctgtcc attccccatg 3120

3061 cgttgtaatg atgtagagaa ttaccggtaa aaggttagag cggggacagg taaggggtac 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttgctgtaga atctctccta ccttgttgac tgtccctcgg cgaatctcct ggcttgaagg 3180

3121 aacgacatct tagagaggat ggaacaactg acagggagcc gcttagagga ccgaacttcc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tccgcgaaga gtcaccagat gtaatttatc tgggccttta aacaatgact tgattatgag 3240

3181 aggcgcttct cagtggtcta cattaaatag acccggaaat ttgttactga actaatactc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctcgagtccg cttcactaga gatactcact gttagcagcg tctgctactg cttccctatt 3300

3241 gagctcaggc gaagtgatct ctatgagtga caatcgtcgc agacgatgac gaagggataa 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 taggtcagca ggagttctgc ttaacagctt tctattgctc tagcttcact tcctcaatca 3360

3301 atccagtcgt cctcaagacg aattgtcgaa agataacgag atcgaagtga aggagttagt 3360

.......... .......... .......... .......... .......... ..........

.......... .......... NarI(3384) .......... .......... ..........

3361 ctctcaatca agtccctgtt cgggcgccaa ctgcgaagtt ctcggcccgg attccgagac 3420

3361 gagagttagt tcagggacaa gcccgcggtt gacgcttcaa gagccgggcc taaggctctg 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctcacaggta aaaaagatta cacagatact cgacagggtt caatctcaaa tatttattgt 3480

3421 gagtgtccat tttttctaat gtgtctatga gctgtcccaa gttagagttt ataaataaca 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 atctgtggga gcctcaaggg agaactcaaa agtcctcaac aaagagactc ctcgaagttt 3540

3481 tagacaccct cggagttccc tcttgagttt tcaggagttg tttctctgag gagcttcaaa 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cacagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaac 3600

3541 gtgtctcgag acgaatatat ctggagggtg gcatgtgcgg atggcgggta a acgcagttg 3600

.......... .......... .. CMV_immearly_promoter CMV_fwd_primer ..

.......... .......... .......... .......... .......... ..........

3601 ggggcggggt tattacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc 3660

3601 ccccgcccca ataatgctgt aaaacctttc agggcaacta aaaccacggt tttgtttgag 3660

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc 3720

3661 ggtaactgca gttaccccac ctctgaacct ttaggggcac tcagtttggc gataggtgcg 3720

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccattggtgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg 3780

3721 ggtaaccaca tgacggtttt ggcgtagtgg taccattatc gctactgatt atgcatctac 3780

.......... .... CMV_immearly_promoter ... CAG_enhancer .........

.......... .......... .......... .......... .......... ..........

3781 tactgccaag taggaaagtc ccgtaaggtc atgtactggg cataatgcca ggcgggccat 3840

3781 atgacggttc atcctttcag ggcattccag tacatgaccc gtattacggt ccgcccggta 3840

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

3841 ttaccgtcat tgacgtcaat agggggcgga cttggcatat gatacacttg atgtactgcc 3900

3841 aatggcagta actgcagtta tcccccgcct gaaccgtata ctatgtgaac tacatgacgg 3900

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

3901 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc 3960

3901 ttcacccgtc aaatggcatt tatgaggtgg gtaactgcag ttacctttca gggataaccg 3960

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

3961 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag 4020

3961 caatgatacc cttgtatgca gtaataactg cagttacccg cccccagcaa cccgccagtc 4020

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

4021 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact 4080

4021 ggtccgcccg gtaaatggca ttcaatacat tgcgccttga ggtatatacc cgatacttga 4080

... CAG_enhancer CMV_immearly_promoter ... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgcca acatggcggt 4140

4081 ttactggggc attaactaat gataattatt gatcagttat tagttacggt tgtaccgcca 4140

.... CMV_immearly_promoter .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 catattggac atgagccaat ataaatgtac atattatgat atagatacaa cgtatgcaat 4200

4141 gtataacctg tactcggtta tatttacatg tataatacta tatctatgtt gcatacgtta 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ggccaatagc caatattgat ttatgctata taaccaatga ataatatggc taatggccaa 4260

4201 ccggttatcg gttataacta aatacgatat attggttact tattataccg attaccggtt 4260

.......... .......... .......... .......... .......... ..........

BglII(4267) ........ .......... .......... .......... ..........

4261 tattgaagat ctgtcgagcc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 4320

4261 ataacttcta gacagctcgg tacactcgtt ttccggtcgt tttccggtcc ttggcatttt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 4380

4321 tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg actgctcgta gtgtttttag 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 4440

4381 ctgcgagttc agtctccacc gctttgggct gtcctgatat ttctatggtc cgcaaagggg 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccggc tttccccgtc 4500

4441 gaccttcgag ggagcacgcg agaggacaag gctgggacgg cgaatggccg aaaggggcag 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 4560

4501 ttcgagattt agcccccgag ggaaatccca aggctaaatc acgaaatgcc gtggagctgg 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt 4620

4561 ggttttttga actaaaccca ctaccaagtg catcacccgg tagcgggact atctgccaaa 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 4680

4621 aagcgggaaa ctgcaacctc aggtgcaaga aattatcacc tgagaacaag gtttgacctt 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg 4740

4681 gttgtgagtt gggatagagc ccgataagaa aactaaatat tccctaaaac ggctaaagcc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 4800

4741 ggataaccaa ttttttactc gactaaattg tttttaaatt gcgcttaaaa ttgttttata 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 4860

4801 attgcaaatg ttaaaatacc acgtgagagt catgttagac gagactacgg cgtatcaatt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 4920

4861 cggtcggggc tgtgggcggt tgtgggcgac tgcgcgggac tgcccgaaca gacgagggcc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 4980

4921 gtaggcgaat gtctgttcga cactggcaga ggccctcgac gtacacagtc tccaaaagtg 4980

.......... .......... .......... ........ pGEX_3_primer ........

.......... .......... .......... .......... .......... ..........

4981 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta 5040

4981 gcagtagtgg ctttgcgcgc tctgctttcc cggagcacta tgcggataaa aatatccaat 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 5100

5041 tacagtacta ttattaccaa agaatctgca gtccaccgtg aaaagcccct ttacacgcgc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 5160

5101 cttggggata aacaaataaa aagatttatg taagtttata cataggcgag tactctgtta 5160

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

5161 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 5220

5161 ttgggactat ttacgaagtt attataactt tttccttctc atactcataa gttgtaaagg 5220

.......... .......... .......... .......... .... Orf frame 3 Ampicillin

.......... .......... .......... .......... .......... ..........

5221 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 5280

5221 cacagcggga ataagggaaa aaacgccgta aaacggaagg acaaaaacga gtgggtcttt 5280

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 5340

5281 gcgaccactt tcattttcta cgacttctag tcaacccacg tgctcaccca atgtagcttg 5340

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 5400

5341 acctagagtt gtcgccattc taggaactct caaaagcggg gcttcttgca aaaggttact 5400

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 5460

5401 actcgtgaaa atttcaagac gatacaccgc gccataatag ggcataactg cggcccgttc 5460

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 5520

5461 tcgttgagcc agcggcgtat gtgataagag tcttactgaa ccaactcatg agtggtcagt 5520

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 5580

5521 gtcttttcgt agaatgccta ccgtactgtc attctcttaa tacgtcacga cggtattggt 5580

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 5640

5581 actcactatt gtgacgccgg ttgaatgaag actgttgcta gcctcctggc ttcctcgatt 5640

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 5700

5641 ggcgaaaaaa cgtgttgtac cccctagtac attgagcgga actagcaacc cttggcctcg 5700

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 5760

5701 acttacttcg gtatggtttg ctgctcgcac tgtggtgcta cggacatcgt taccgttgtt 5760

.......... .... Orf frame 3 Ampicillin .... .......... ..........

FspI(5766) .......... .......... .......... .......... ..........

5761 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 5820

5761 gcaacgcgtt tgataattga ccgcttgatg aatgagatcg aagggccgtt gttaattatc 5820

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 5880

5821 tgacctacct ccgcctattt caacgtcctg gtgaagacgc gagccgggaa ggccgaccga 5880

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 5940

5881 ccaaataacg actatttaga cctcggccac tcgcacccag agcgccatag taacgtcgtg 5940

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 6000

5941 accccggtct accattcggg agggcatagc atcaatagat gtgctgcccc tcagtccgtt 6000

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 6060

6001 gatacctact tgctttatct gtctagcgac tctatccacg gagtgactaa ttcgtaacca 6060

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 6120

6061 ttgacagtct ggttcaaatg agtatatatg aaatctaact aaattttgaa gtaaaaatta 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 6180

6121 aattttccta gatccacttc taggaaaaac tattagagta ctggttttag ggaattgcac 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 6240

6181 tcaaaagcaa ggtgactcgc agtctggggc atcttttcta gtttcctaga agaactctag 6240

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

6241 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 6300

6241 gaaaaaaaga cgcgcattag acgacgaacg tttgtttttt tggtggcgat ggtcgccacc 6300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6301 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 6360

6301 aaacaaacgg cctagttctc gatggttgag aaaaaggctt ccattgaccg aagtcgtctc 6360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 6420

6361 gcgtctatgg tttatgacag gaagatcaca tcggcatcaa tccggtggtg aagttcttga 6420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 6480

6421 gacatcgtgg cggatgtatg gagcgagacg attaggacaa tggtcaccga cgacggtcac 6480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 6540

6481 cgctattcag cacagaatgg cccaacctga gttctgctat caatggccta ttccgcgtcg 6540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 6600

6541 ccagcccgac ttgcccccca agcacgtgtg tcgggtcgaa cctcgcttgc tggatgtggc 6600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6601 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 6660

6601 ttgactctat ggatgtcgca ctcgatactc tttcgcggtg cgaagggctt ccctctttcc 6660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6661 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 6720

6661 gcctgtccat aggccattcg ccgtcccagc cttgtcctct cgcgtgctcc ctcgaaggtc 6720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6721 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 6780

6721 cccctttgcg gaccatagaa atatcaggac agcccaaagc ggtggagact gaactcgcag 6780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6781 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 6840

6781 ctaaaaacac tacgagcagt ccccccgcct cggatacctt tttgcggtcg ttgcgccgga 6840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6841 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 6900

6841 aaaatgccaa ggaccggaaa acgaccggaa aacgagtgta caagaaagga cgcaataggg 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 6960

6901 gactaagaca cctattggca taatggcgga aactcactcg actatggcga gcggcgtcgg 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag........ .......... 7002

6961 cttgctggct cgcgtcgctc agtcactcgc tccttcgcct tc........ .......... 7002

.......... .......... .......... .......... .......... ..........