Gene/insert name: CaMV 35S enhancers
Insert size (bp): Unknown
Vector backbone: pPZP212
Backbone manufacturer: Hajdukiewicz et al 1994
Type of vector: Plant expression
Backbone size (bp): 11073
Cloning site 5':
Site destroyed during cloning: No
Cloning site 3': See paper and map.
Site destroyed during cloning: No
5' Sequencing primer: M13 reverse
Bacteria resistance: Spectinomycin
High or low copy: High Copy
Grow in standard E. coli @ 37C: No
Please specify bacterial strain for growth and growth condition: Plasmid grow in standard E. coli at 37 degrees, but the four enhancer repeats may be unstable in E. coli and A. tumefaciens if stored at 4'C for extended time. See comments. Selectable markers: Neomycin If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: Max Planck Institute Plasmid Provided In: DH5a
Principal Investigator: Detlef Weigel
Comments: Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article. Kanamycin gene for plant selection on MS media. Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l).
Article: Activation tagging in Arabidopsis. Weigel D et al. (Plant Physiol. 2000 Apr . 122(4):1003-13. Pubmed)