Sequence of pMN19

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Sequence of pMN19

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Sequence of pMN19 : 11073 BP; 2728 A; 2984 C; 2985 G; 2376 T; 0 other;

.......... ...... SmaI(21) KpnI(27) SacII(40) ...... SpeI(59)

1 ggtcgactct agaggatccc cgggtaccga gctccaccgc ggtggcggcc gctctagaac 60

1 ccagctgaga tctcctaggg gcccatggct cgaggtggcg ccaccgccgg cgagatcttg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tagtggatcc ccaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat 120

61 atcacctagg ggttgtacca cctcgtgctg tgagagcaga tgaggttctt atagtttcta 120

pBlueSK_pri ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 acagtctcag aagaccagag ggctattgag acttttcaac aaagggtaat atcgggaaac 180

121 tgtcagagtc ttctggtctc ccgataactc tgaaaagttg tttcccatta tagccctttg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt agaaaaggaa 240

181 gaggagccta aggtaacggg tcgatagaca gtgaagtagc tttcctgtca tcttttcctt 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gatggcttct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct 300

241 ctaccgaaga tgtttacggt agtaacgcta tttcctttcc gatagcaagt tctacggaga 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 accgacagtg gtcccaaaga tggaccccca cccacgagga acatcgtgga aaaagaagac 360

301 tggctgtcac cagggtttct acctgggggt gggtgctcct tgtagcacct ttttcttctg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctagatccc caacatggtg 420

361 caaggttggt gcagaagttt cgttcaccta actacactat agatctaggg gttgtaccac 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccagagg 480

421 ctcgtgctgt gagagcagat gaggttctta tagtttctat gtcagagtct tctggtctcc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca 540

481 cgataactct gaaaagttgt ttcccattat agccctttgg aggagcctaa ggtaacgggt 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gctatctgtc acttcatcga aaggacagta gaaaaggaag atggcttcta caaatgccat 600

541 cgatagacag tgaagtagct ttcctgtcat cttttccttc taccgaagat gtttacggta 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cattgcgata aaggaaaggc tatcgttcaa gatgcctcta ccgacagtgg tcccaaagat 660

601 gtaacgctat ttcctttccg atagcaagtt ctacggagat ggctgtcacc agggtttcta 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ggacccccac ccacgaggaa catcgtggaa aaagaagacg ttccaaccac gtcttcaaag 720

661 cctgggggtg ggtgctcctt gtagcacctt tttcttctgc aaggttggtg cagaagtttc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 caagtggatt gatgtgatat ctagatcccc aacatggtgg agcacgacac tctcgtctac 780

721 gttcacctaa ctacactata gatctagggg ttgtaccacc tcgtgctgtg agagcagatg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa 840

781 aggttcttat agtttctatg tcagagtctt ctggtctccc gataactctg aaaagttgtt 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa 900

841 tcccattata gccctttgga ggagcctaag gtaacgggtc gatagacagt gaagtagctt 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct 960

901 tcctgtcatc ttttccttct accgaagatg tttacggtag taacgctatt tcctttccga 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac 1020

961 tagcaagttc tacggagatg gctgtcacca gggtttctac ctgggggtgg gtgctccttg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc 1080

1021 tagcaccttt ttcttctgca aggttggtgc agaagtttcg ttcacctaac tacactatag 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tagatcccca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca 1140

1081 atctaggggt tgtaccacct cgtgctgtga gagcagatga ggttcttata gtttctatgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc 1200

1141 cagagtcttc tggtctcccg ataactctga aaagttgttt cccattatag ccctttggag 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat 1260

1201 gagcctaagg taacgggtcg atagacagtg aagtagcttt cctgtcatct tttccttcta 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc 1320

1261 ccgaagatgt ttacggtagt aacgctattt cctttccgat agcaagttct acggagatgg 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gacagtggtc ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt 1380

1321 ctgtcaccag ggtttctacc tgggggtggg tgctccttgt agcacctttt tcttctgcaa 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccaaccacgt cttcaaagca agtggattga tgtgatatct agatccgaaa ctatcagtgt 1440

1381 ggttggtgca gaagtttcgt tcacctaact acactataga tctaggcttt gatagtcaca 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttgagaaggc tatttatcca tttctgaaaa ggcgaaatgg cgtcaccgcg agcgtcacgc 1500

1441 aactcttccg ataaataggt aaagactttt ccgctttacc gcagtggcgc tcgcagtgcg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gcattccgtt cttgctgtaa agcgttgttt ggtacacttt tgaatagcga ggcttggcgt 1560

1501 cgtaaggcaa gaacgacatt tcgcaacaaa ccatgtgaaa acttatcgct ccgaaccgca 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gtcagcgtat ctattcaaaa gtcgttaatg gctgcggatc aagaaaaagt tggaatagaa 1620

1561 cagtcgcata gataagtttt cagcaattac cgacgcctag ttctttttca accttatctt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 acagaatacc cgcgaaattc aggcccggtt gccatgtcct acacgccgaa ataaacgacc 1680

1621 tgtcttatgg gcgctttaag tccgggccaa cggtacagga tgtgcggctt tatttgctgg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aaattagtag aaaaataaaa actgactcgg atacttacgt cacgtcttgc gcactgattt 1740

1681 tttaatcatc tttttatttt tgactgagcc tatgaatgca gtgcagaacg cgtgactaaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gaaaaatctc aatataaaca aagacggcca caagaaaaaa ccaaaacacc gatattcatt 1800

1741 ctttttagag ttatatttgt ttctgccggt gttctttttt ggttttgtgg ctataagtaa 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatcttatct agtttctcaa aaaaattcat atcttccaca cgtgaaaatg ccaatttctc 1860

1801 ttagaataga tcaaagagtt tttttaagta tagaaggtgt gcacttttac ggttaaagag 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agacctacct cggctctgcg aaggcccccg ctggtatcaa aagtttttat ttcatccgac 1920

1861 tctggatgga gccgagacgc ttccgggggc gaccatagtt ttcaaaaata aagtaggctg 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atggcgcgac cgacctcaac gagaaggaaa ttgtcgtgaa cggtgagaag ctctggggcg 1980

1921 taccgcgctg gctggagttg ctcttccttt aacagcactt gccactcttc gagaccccgc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgcaaggttc cggaacgaac atcggtctca atgcaaaagg ggaacgccag gctctgttgg 2040

1981 acgttccaag gccttgcttg tagccagagt tacgttttcc ccttgcggtc cgagacaacc 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... EcoRI(2094)

2041 cccctcgagg tcgacggtat cgataagctt atcgatatct agatctcgag ctcgaattcg 2100

2041 ggggagctcc agctgccata gctattcgaa tagctataga tctagagctc gagcttaagc 2100

......... pBlueKS_pri ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 2160

2101 attagtacca gtatcgacaa aggacacact ttaacaatag gcgagtgtta aggtgtgttg 2160

.......... M13_r .......... M13_pUC_r .......... ..........

.......... .......... .......... .......... .......... ..........

2161 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 2220

2161 tatgctcggc cttcgtattt cacatttcgg accccacgga ttactcactc gattgagtgt 2220

.......... lac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 2280

2221 aattaacgca acgcgagtga cgggcgaaag gtcagccctt tggacagcac ggtcgacgta 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag cagcttgagc 2340

2281 attacttagc cggttgcgcg cccctctccg ccaaacgcat aaccgatctc gtcgaactcg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat 2400

2341 aacctagtct aacagcaaag ggcggaagtc aaatttgata gtcacaaact gtcctatata 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt 2460

2401 accgcccatt tggattctct tttctcgcaa ataatcttat tgcctataaa ttttcccgca 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg 2520

2461 cttttccaaa taggcaagca ggtaaacata cacgtacggt tggtgtccca aggggagccc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag 2580

2521 tagtttcatg aaactaggtt ggggaggcga cgatatcacg tcagccgaag actgcaagtc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg 2640

2581 acgtcggcag aagacttttg ctgtacagcg tgttcaggat tcaatgcgct gtccgacggc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt 2700

2641 gggacgggaa aaggaccgca aaagaacagc gcacaaaatc agcgtatttc atcttatgaa 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct 2760

2701 cgctgatctt ggcctctgta atgcggtact tgttctcgcg gcggcgaccg gacgacccga 2760

.......... .......... .......... .......... ORF3 ..........

.......... .......... .......... .......... .......... ..........

2761 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg 2820

2761 tacgggcgca gtcgtggctg ctggtcctga actggttggt tgcccggctt gacgtgcgcc 2820

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc 2880

2821 ggccgacgtg gttcgacaaa aggctcttct agtggccgtg gtccgcgctg gcgggcctcg 2880

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc 2940

2881 accggtccta cgaactggtg gatgcgggac cgctgcaaca ctgtcactgg tccgatctgg 2940

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg 3000

2941 cggaccgggc gtcgtgggcg ctggatgacc tgtaacggct cgcgtaggtc ctccggccgc 3000

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg 3060

3001 gcccggacgc atcggaccgt ctcggcaccc ggctgtggtg gtgcggccgg ccggcgtacc 3060

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc 3120

3061 acaactggca caagcggccg taacggctca agctcgcaag ggattagtag ctggcgtggg 3120

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca 3180

3121 cctcgcccgc gctccggcgg ttccgggctc cgcacttcaa accgggggcg ggatgggagt 3180

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag 3240

3181 ggggccgtgt ctagcgcgtg cgggcgctcg actagctggt ccttccggcg tggcactttc 3240

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg 3300

3241 tccgccgacg tgacgaaccg cacgtagcga gctgggacat ggcgcgtgaa ctcgcgtcgc 3300

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg 3360

3301 tccttcactg cgggtggctc cggtccgccg cgccacggaa ggcactcctg cgtaactggc 3360

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca 3420

3361 tccggctgcg ggaccgccgg cggctcttac ttgcggttct ccttgttcgt actttggcgt 3420

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc 3480

3421 ggtcctgccg gtcctgcttg gcaaaaagta atggcttctc tagctccgcc tctactagcg 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct 3540

3481 ccggcccatg cacaagctcg gcgggcgcgt gcagagttgg cacgccgacg tactttagga 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac 3600

3541 ccggccaaac agactacggt tcgaccgccg gaccggccgg tcgaaccggc gacttctttg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt 3660

3601 gctcgcggcg gcagattttt ccactacaca taaactcatt ttgtcgaacg cagtacgcca 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt 3720

3661 gcgacgcata tactacgcta ctcatttatt tgtttatgcg ttccccttgc gtacttccaa 3720

.......... .......... .......... .......... .......... ORF1

.......... .......... .......... .......... .......... ..........

3721 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc 3780

3721 tagcgacatg aattggtctt tccgcccagt ccgttctgct ggtagcgttg ggtagatcgg 3780

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt 3840

3781 gcgcgggacg ttgagcggcc ccggctacaa gacaatcagc taaggctagg ggtcccgtca 3840

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc 3900

3841 cgggcgctaa cccgccggca cgcccttcta gttggcgatt ggcaacagcc gtagctggcg 3900

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga 3960

3901 ggctgctaac tggcgctgca cttccggtag ccggccgcgc tgaagcatca ctagctgcct 3960

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt 4020

3961 cgcggggtcc gccgcctgaa ccgacacagg cgctagttcc gtcggctgaa gcacgactaa 4020

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag 4080

4021 ggccacgtcg gttcgggaat gctgtatacc cggtggcggc tggaccacct cgaccaattc 4080

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc 4140

4081 gtcgcgtaac tccagtgcct accttccgat gttcgccgga aacagcacag cgcccgctag 4140

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt 4200

4141 tttccgtgcg cgtagccgcc actccaacgg ctccgcgacc ggcccatgct cgacgggtaa 4200

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc 4260

4201 gaactcaggg catagtgcgt cgcgcactcg atgggtccgt gacggcggcg gccgtgttgg 4260

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa 4320

4261 caagaactta gtcttgggct cccgctgcga cgggcgctcc aggtccgcga ccggcgactt 4320

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca 4380

4321 taatttagtt ttgagtaaac tcaattactc catttctctt ttactcgttt tcgtgtttgt 4380

.......... ORF1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg 4440

4381 gcgattcacg gccggcaggc tcgcgtgcgt cgtcgttccg acgttgcaac cggtcggacc 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc 4500

4441 gtctgtgcgg tcggtacttc gcccagttga aagtcaacgg ccgcctccta gtgtggttcg 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg 4560

4501 acttctacat gcgccatgcg gttccgttct ggtaatggct cgacgataga cttatgtagc 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa 4620

4561 gcgtcgatgg tctcatttac tcgtttactt atttactcat ctacttaaaa tcgccgattt 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt 4680

4621 cctccgccgt accttttagt tcttgttggt ccgtggctgc ggcaccttac ggggtacaca 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc 4740

4681 cctccttgcc cgccaaccgg tccgcattcg ccgacccaac agacggccgg gacgttaccg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca 4800

4741 tgaccttggg ggttcgggct ccttagccgc actgccagcg tttggtaggc cgggccatgt 4800

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca 4860

4801 ttagccgcgc cgcgacccac tactggacca cctcttcaac ttccggcgcg tccggcgggt 4860

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg 4920

4861 cgccgttgcg tagctccgtc ttcgtgcggg gccacttagc accgttcgcc ggcgactagc 4920

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc 4980

4921 ttaggcgttt cttagggccg ttggcggccg tcggccacgc ggcagctaat ccttcggcgg 4980

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga 5040

4981 gttcccgctg ctcgttggtc taaaaaagca aggctacgag atactgcacc cgtgggcgct 5040

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg 5100

5041 atcagcgtcg tagtacctgc accggcaaaa ggcagacagc ttcgcactgg ctgctcgacc 5100

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg 5160

5101 gctccactag gcgatgctcg aaggtctgcc cgtgcatctc caaaggcgtc ccggccggcc 5160

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc 5220

5161 gtaccggtca cacaccctaa tgctggacca tgactaccgc caaagggtag attggcttag 5220

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt 5280

5221 gtacttggct atggcccttc ccttccctct gttcgggccg gcgcacaagg caggtgtgca 5280

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt 5340

5281 acgcctgcat gagttcaaga cggccgctcg gctaccgcct ttcgtctttc tgctggacca 5340

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa 5400

5341 tctttggacg taagccaatt tgtggtgcgt gcaacggtac gtcgcatgct tcttccggtt 5400

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt 5460

5401 cttgccggcg gaccactgcc ataggctccc acttcggaac taatcggcga tgttctagca 5460

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... NheI(5500) .......... ..........

5461 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg 5520

5461 tttctcgctt tggcccgccg gcctcatgta gctctagctc gatcgactaa cctacatggc 5520

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat 5580

5521 gctctagtgt cttccgttct tgggcctgca cgactgccaa gtggggctaa tgaaaaacta 5580

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga 5640

5581 gctagggccg tagccggcaa aagagatggc ggaccgtgcg gcgcggcgtc cgttccgtct 5640

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa 5700

5641 tcggtctacc aacaagttct gctagatgct tgcgtcaccg tcgcggcctc tcaagttctt 5700

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa 5760

5701 caagacaaag tggcacgcgt tcgactagcc cagtttactg gacggcctca tgctaaactt 5760

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg 5820

5761 cctcctccgc cccgtccgac cgggctagga tcagtacgcg atggcgttgg actagctccc 5820

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg 5880

5821 gcttcgtagg cggccaagga ttacatgcct cgtctacgat cccgtttaac gggatcgtcc 5880

.......... ORF2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc 5940

5881 cctttttcca gcttttccag agaaaggaca cctatcgtgc atgtaaccct tgggtttcgg 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... AgeI(5994)

5941 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc 6000

5941 catgtaaccc ttggccttgg gcatgtaacc cttgggtttc ggcatgtaac ccttggccag 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt 6060

6001 tgtgtacatt cactgactat attttctctt ttttccgcta aaaaggcgga ttttgagaaa 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac 6120

6061 ttttgaataa ttttgagaat tttgggcgga ccggacacgt attgacagac cggtcgcgtg 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc 6180

6121 tcggcttctc gacgtttttc gcggatggga agccagcgac gcgagggatg cggggcggcg 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa 6240

6181 aagcgcagcc ggatagcgcc ggcgaccggc gagtttttac cgaccggatg ccggtccgtt 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg 6300

6241 agatggtccc gcgcctgttc ggcgcggcag cggtgagctg gcggccgcgg gtgtagttcc 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 6360

6301 gtgggacgga gcgcgcaaag ccactactgc cacttttgga gactgtgtac gtcgagggcc 6360

.......... .......... .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

6361 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 6420

6361 tctgccagtg tcgaacagac attcgcctac ggccctcgtc tgttcgggca gtcccgcgca 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag 6480

6421 gtcgcccaca accgcccaca gccccgcgtc ggtactgggt cagtgcatcg ctatcgcctc 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 6540

6481 acatatgacc gaattgatac gccgtagtct cgtctaacat gactctcacg tggtatacgc 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc 6600

6541 cacactttat ggcgtgtcta cgcattcctc ttttatggcg tagtccgcga gaaggcgaag 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 6660

6601 gagcgagtga ctgagcgacg cgagccagca agccgacgcc gctcgccata gtcgagtgag 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 6720

6661 tttccgccat tatgccaata ggtgtcttag tcccctattg cgtcctttct tgtacactcg 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 6780

6721 ttttccggtc gttttccggt ccttggcatt tttccggcgc aacgaccgca aaaaggtatc 6780

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

6781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 6840

6781 cgaggcgggg ggactgctcg tagtgttttt agctgcgagt tcagtctcca ccgctttggg 6840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 6900

6841 ctgtcctgat atttctatgg tccgcaaagg gggaccttcg agggagcacg cgagaggaca 6900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 6960

6901 aggctgggac ggcgaatggc ctatggacag gcggaaagag ggaagccctt cgcaccgcga 6960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 7020

6961 aagagtatcg agtgcgacat ccatagagtc aagccacatc cagcaagcga ggttcgaccc 7020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 7080

7021 gacacacgtg cttggggggc aagtcgggct ggcgacgcgg aataggccat tgatagcaga 7080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 7140

7081 actcaggttg ggccattctg tgctgaatag cggtgaccgt cgtcggtgac cattgtccta 7140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 7200

7141 atcgtctcgc tccatacatc cgccacgatg tctcaagaac ttcaccaccg gattgatgcc 7200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 7260

7201 gatgtgatct tcctgtcata aaccatagac gcgagacgac ttcggtcaat ggaagccttt 7260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7261 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 7320

7261 ttctcaacca tcgagaacta ggccgtttgt ttggtggcga ccatcgccac caaaaaaaca 7320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7321 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 7380

7321 aacgttcgtc gtctaatgcg cgtctttttt tcctagagtt cttctaggaa actagaaaag 7380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7381 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga 7440

7381 atgccccaga ctgcgagtca ccttgctttt gagtgcaatt ccctaaaacc agtacgtact 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 tatatctccc aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt 7500

7441 atatagaggg ttaaacacat cccgaataat acgtgcgaat ttttattatt ttcgtctgaa 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... HpaI(7557)

7501 gacctgatag tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac 7560

7501 ctggactatc aaaccgacac tcgttaatac acgaatcacg tagattagcg aactcaattg 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 gccggcgaag cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct 7620

7561 cggccgcttc gccgcagccg aacttgctta aagatcgatc tgtaataaac ggctgatgga 7620

.......... .......... .......... .......... .......... ORF2 spect

.......... .......... .......... .......... .......... ..........

7621 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca 7680

7621 accactagag cggaaagtgc atcacctgtt taagaaggtt gactagacgc gcgctccggt 7680

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact 7740

7681 tcgctagaag aagaacaggt tctattcgga cagatcgaag ttcatactgc ccgactatga 7740

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg 7800

7741 cccggccgtc cgcgaggtaa cgggtcagcc gtcgctgtag gaagccgcgc taaaacggcc 7800

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc 7860

7801 aatgacgcga catggtttac gccctgttgc attcgtgatg taaagcgagt agcggtcggg 7860

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt 7920

7861 tcagcccgcc gctcaaggta tcgcaattcc aaagtaaatc gcggagttta tctaggacaa 7920

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc 7980

7921 gtccttggcc tagtttctca aggaggcggc gacctggatg gttccgttgc gatacaagag 7980

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg 8040

7981 aacgaaaaca gtcgttctat cggtctagtt acagctagca ccgaccgagc ttctatggac 8040

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc 8100

8041 gttcttacag taacgcgacg gtaagaggtt taacgtcaag cgcgaatcga cctattgcgg 8100

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct 8160

8101 tgccttacta cagcagcacg tgttgttacc actgaagatg tcgcgcctct tagagcgaga 8160

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat 8220

8161 gaggtcccct tcggcttcaa aggttttcca gcaactagtt tcgagcggcg caacaaagta 8220

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat 8280

8221 gttcggaatg ccagtggcat tggtcgttta gttatagtga cacaccgaag tccggcggta 8280

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga 8340

8281 ggtgacgcct cggcatgttt acatgccggt cgttgcagcc aagctctacc gcgagctact 8340

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt 8400

8341 gcggttgatg gagactatca actcagctat gaagccgcta gtggcgaagg gggtactaca 8400

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca 8460

8401 aattgaaaca aaatcccgct gacgggacga cgcattgtag caacgacgag gtattgtagt 8460

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc 8520

8461 ttgtagctgg gtgccgcatt gcgcgaacga cgaacctacg ggctccgtat ctgacatggg 8520

.......... .......... ORF2 spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 caaaaaaaca tgtcataaca agaagccatg aaaaccgcca ctgcgccgtt accaccgctg 8580

8521 gtttttttgt acagtattgt tcttcggtac ttttggcggt gacgcggcaa tggtggcgac 8580

ORF2 .......... spect .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 cgttcggtca aggttctgga ccagttgcgt gacggcagtt acgctacttg cattacagct 8640

8581 gcaagccagt tccaagacct ggtcaacgca ctgccgtcaa tgcgatgaac gtaatgtcga 8640

.......... spect .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 tacgaaccga acgaggctta tgtccactgg gttcgtgccc gaattgatca caggcagcaa 8700

8641 atgcttggct tgctccgaat acaggtgacc caagcacggg cttaactagt gtccgtcgtt 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc 8760

8701 gcgagacagt agcaatgtta gttgtacgat gggaggcgct ctagtaggca caaagtttgg 8760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct 8820

8761 gccgtcgaat caacggcaag aaggcttatc gtagccattg tactcgtttc agacggcgga 8820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga 8880

8821 atgttgccga gagggcgact gcggcagggc ctgactaccc gacggacata gctcaccact 8880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg 8940

8881 aaaacacggc tcgacggcca gcccctcgac aaccgaccga ccaccgtcct atataacacc 8940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg 9000

8941 acatttgttt aactgcgaat ctgttgaatt attgtgtaac gcctgcaaaa attacatgac 9000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 aattaacgcc gaattaattc gggggatcta tagggacttt aggtgatctg gattttagta 9060

9001 ttaattgcgg cttaattaag ccccctagat atccctgaaa tccactagac ctaaaatcat 9060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat 9120

9061 gacctaaaac caaaatcctt aatctttaaa ataactatct tcataaaatg tttatgttta 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 acatactaag ggtttcttat atgctcaaca cgtgagcgaa accctataag aaccctaatt 9180

9121 tgtatgattc ccaaagaata tacgagttgt gcactcgctt tgggatattc ttgggattaa 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 cccttatctg ggaactactc acacattatt atggagaaac tcgatgtcga tcgactctag 9240

9181 gggaatagac ccttgatgag tgtgtaataa tacctctttg agctacagct agctgagatc 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag 9300

9241 gatctcctag ctaggcttgg ggtctcaggg cgagtcttct tgagcagttc ttccgctatc 9300

.......... .......... .......... .... ORF3 Neomycin(variant) ....

.......... .......... .......... .......... .......... ..........

9301 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc 9360

9301 ttccgctacg cgacgcttag ccctcgccgc tatggcattt cgtgctcctt cgccagtcgg 9360

.......... .... ORF3 Neomycin(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg 9420

9361 gtaagcggcg gttcgagaag tcgttatagt gcccatcggt tgcgatacag gactatcgcc 9420

.......... .... ORF3 Neomycin(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg 9480

9421 aggcggtgtg ggtcggccgg tgtcagctac ttaggtcttt tcgccggtaa aaggtggtac 9480

.......... .... ORF3 Neomycin(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc gggcatgcgc 9540

9481 tataagccgt tcgtccgtag cggtacacag tgctgctcta ggagcggcag cccgtacgcg 9540

.......... .... ORF3 Neomycin(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca 9600

9541 cggaactcgg accgcttgtc aagccgaccg cgctcgggga ctacgagaag caggtctagt 9600

.......... .... ORF3 Neomycin(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct 9660

9601 aggactagct gttctggccg aaggtaggct catgcacgag cgagctacgc tacaaagcga 9660

.......... .... ORF3 Neomycin(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc 9720

9661 accaccagct tacccgtcca tcggcctagt tcgcatacgt cggcggcgta acgtagtcgg 9720

.......... .... ORF3 Neomycin(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact 9780

9721 tactacctat gaaagagccg tcctcgttcc actctactgt cctctaggac ggggccgtga 9780

.......... .... ORF3 Neomycin(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa 9840

9781 agcgggttat cgtcggtcag ggaagggcga agtcactgtt gcagctcgtg tcgacgcgtt 9840

.......... .... ORF3 Neomycin(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag ttcattcagg 9900

9841 ccttgcgggc agcaccggtc ggtgctatcg gcgcgacgga gcaggacctc aagtaagtcc 9900

.......... .... ORF3 Neomycin(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac 9960

9901 cgtggcctgt ccagccagaa ctgtttttct tggcccgcgg ggacgcgact gtcggccttg 9960

.......... .... ORF3 Neomycin(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc 10020

9961 tgccgccgta gtctcgtcgg ctaacagaca acacgggtca gtatcggctt atcggagagg 10020

.......... .... ORF3 Neomycin(variant) .... .......... ..........

.......... .......... .......... ......... NcoI(10067) .........

10021 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat ggtcgatcga 10080

10021 tgggttcgcc ggcctcttgg acgcacgtta ggtagaacaa gttaggggta ccagctagct 10080

...... Neomycin(variant) ORF3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 cagatctgcg aatcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc 10140

10081 gtctagacgc ttagctctct ctatctaaac atctctctct gaccactaaa gtcgcacagg 10140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt 10200

10141 agaggtttac tttacttgaa ggaatatatc tccttcccag aacgcttcct atcaccctaa 10200

.......... ......... CaMVd3S_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10201 gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg 10260

10201 cacgcagtag ggaatgcagt cacctctata gtgtagttag gtgaacgaaa cttctgcacc 10260

.......... ......... CaMVd3S_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac 10320

10261 aaccttgcag aagaaaaagg tgctacgagg agcacccacc cccaggtaga aaccctggtg 10320

.......... ......... CaMVd3S_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg 10380

10321 acagccgtct ccgtagaact tgctatcgga aaggaaatag cgttactacc gtaaacatcc 10380

.......... ......... CaMVd3S_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc 10440

10381 acggtggaag gaaaagatga caggaaaact acttcactgt ctatcgaccc gttaccttag 10440

.......... ......... CaMVd3S_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg 10500

10441 gctcctccaa agggctataa tgggaaacaa cttttcagag ttatcgggaa accagaagac 10500

.......... ......... CaMVd3S_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttatcac 10560

10501 tctgacatag aaactataag aacctcatct gctctcacag cacgaggtgg tacaatagtg 10560

.......... ......... CaMVd3S_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg 10620

10561 tagttaggtg aacgaaactt ctgcaccaac cttgcagaag aaaaaggtgc tacgaggagc 10620

.......... ......... CaMVd3S_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10621 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc 10680

10621 acccaccccc aggtagaaac cctggtgaca gccgtctccg tagaacttgc tatcggaaag 10680

.......... ......... CaMVd3S_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10681 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga 10740

10681 gaaatagcgt tactaccgta aacatccacg gtggaaggaa aagatgacag gaaaactact 10740

.......... ......... CaMVd3S_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10741 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa 10800

10741 tcactgtcta tcgacccgtt accttaggct cctccaaagg gctataatgg gaaacaactt 10800

.......... ......... CaMVd3S_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10801 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga 10860

10801 ttcagagtta tcgggaaacc agaagactct gacatagaaa ctataagaac ctcatctgct 10860

.......... ......... CaMVd3S_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10861 gagtgtcgtg ctccaccatg ttggcaagct gctctagcat tcgccattca ggctgcgcaa 10920

10861 ctcacagcac gaggtggtac aaccgttcga cgagatcgta agcggtaagt ccgacgcgtt 10920

......... CaMVd3S_pro ......... .......... .......... lacZ_a

.......... .......... .......... .......... .......... ..........

10921 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 10980

10921 gacaaccctt cccgctagcc acgcccggag aagcgataat gcggtcgacc gctttccccc 10980

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10981 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 11040

10981 tacacgacgt tccgctaatt caacccattg cggtcccaaa agggtcagtg ctgcaacatt 11040

.......... .......... lacZ_a .......... .......... M13_pUC_f

.......... .......... .......... .......... .......... ..........

11041 aacgacggcc agtgccaagc ttgcatgcct gca....... .......... .......... 11073

11041 ttgctgccgg tcacggttcg aacgtacgga cgt....... .......... .......... 11073

lacZ_a M13_f20 ...... .......... .......... .......... ..........