gZgZ~LOCUS pMT/V5-His-TOPO 3558 bp DNA SYN 28-Sep-2007 DEFINITION pMT/V5-His-TOPO ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3558 /organism="pMT/V5-His-TOPO" /mol_type="other DNA" misc_feature complement(29..51) /label="pGEX_3_primer" misc_feature complement(250..393) /label="lacZ_a" misc_feature 364..386 /label="M13_pUC_fwd_primer" misc_feature 379..395 /label="M13_forward20_primer" misc_feature 814..831 /label="Metallothionein_primer" misc_feature 950..991 /label="V5" misc_feature 1001..1018 /label="6xHis" misc_feature complement(1041..1058) /label="BGH_rev_primer" misc_feature complement(1084..1103) /label="EBV_rev_primer" terminator complement(1070..1189) /label="SV40_PA_terminator" misc_feature complement(1334..1352) /label="M13_reverse_primer" misc_feature complement(1351..1373) /label="M13_pUC_rev_primer" promoter complement(1387..1416) /label="lac_promoter" rep_origin complement(1725..2344) /label="pBR322_origin" CDS complement(2499..3359) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(2499..3359) /label="Ampicillin" /gene="Ampicillin" promoter complement(3401..3429) /label="AmpR_promoter" ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAG TGAATTAATT CGTTGCAGGA 421 CAGGATGTGG TGCCCGATGT GACTAGCTCT TTGCTGCAGG CCGTCCTATC CTCTGGTTCC 481 GATAAGAGAC CCAGAACTCC GGCCCCCCAC CGCCCACCGC CACCCCCATA CATATGTGGT 541 ACGCAAGTAA GAGTGCCTGC GCATGCCCCA TGTGCCCCAC CAAGAGTTTT GCATCCCATA 601 CAAGTCCCCA AAGTGGAGAA CCGAACCAAT TCTTCGCGGG CAGAACAAAA GCTTCTGCAC 661 ACGTCTCCAC TCGAATTTGG AGCCGGCCGG CGTGTGCAAA AGAGGTGAAT CGAACGAAAG 721 ACCCGTGTGT AAAGCCGCGT TTCCAAAATG TATAAAACCG AGAGCATCTG GCCAATGTGC 781 ATCAGTTGTG GTCAGCAGCA AAATCAAGTG AATCATCTCA GTGCAACTAA AGGGGGGATC 841 TAGATCGGGG TACCTACTAG TCCAGTGTGG TGGAATTGCC CTTAAGGGCA ATTCTGCAGA 901 TATCCAGCAC AGTGGCGGCC GCTCGAGTCT AGAGGGCCCG CGGTTCGAAG GTAAGCCTAT 961 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GCGTACCGGT CATCATCACC ATCACCATTG 1021 AGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAAG ATCCAGACAT GATAAGATAC 1081 ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA 1141 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC 1201 AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC 1261 AAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC AGTCGACCTG CAGGCATGCA 1321 AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT 1381 CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC 1441 TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC 1501 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT 1561 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA 1621 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC 1681 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT 1741 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG 1801 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC 1861 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC 1921 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC 1981 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC 2041 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT 2101 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT 2161 AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC 2221 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT 2281 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG 2341 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC 2401 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA 2461 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG 2521 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG 2581 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA 2641 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG 2701 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA 2761 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC 2821 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA 2881 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG 2941 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT 3001 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC 3061 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG 3121 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG 3181 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT 3241 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA 3301 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA 3361 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 3421 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 3481 GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT 3541 ATCACGAGGC CCTTTCGT //pMT/V5-His-TOPO "Times New RomangZC3 pGEX_3_pri"Times New RomangZClacZ_a"Times New RomangZCɆl M13_pUC_f"Times New RomangZ{M13_f20"Times New Roman)gZC.? Metal_pri"Times New RomangZCV5"Times New RomangZCɆ6xHis"Times New RomangZ"BGH_r"Times New RomangZC. SV40_PA_term"Times New RomangZC<OEBV_r"Times New RomangZCɆ6HM13_r"Times New RomangZG] M13_pUC_r"Times New RomangZCklac_pro"Times New RomangZC(  pBR322_origin"Times New RomangZCɆ   Orf frame 3"Times New Roman(gZ   Ampicillin"Times New RomangZCI e AmpR_pro"Times New Roman gZNarI"Times New Roman vdQB Q gZMscI"Times New RomanTh gZ KpnI(853), SpeI(856), AflII(881)"Times New RomanXYm gZNEcoRV(901), NotI(916), XhoI(922), ApaI(938), SacII(941), BstBI(945), AgeI(995)"Times New RomanY5m68l gZHpaI"Times New Roman ARcc gZSalI"Times New Roman ,;JJ gZAatII"Times New Roman 05pjlVhBG3B gZtest"Times New RomanWI;D(>(k gZtest"Times New Romand&33( gZtest"Times New Roman)s)FsP