Sequence of pMT_V5-His-TOPO

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Sequence of pMT_V5-His-TOPO

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Sequence of pMT/V5-His-TOPO : 3558 BP; 907 A; 890 C; 889 G; 872 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60

1 agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc ctctgccagt 60

.......... .......... .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120

61 gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180

121 aaccgcccac agccccgacc gaattgatac gccgtagtct cgtctaacat gactctcacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NarI(236)

181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240

181 tggtatacgc cacactttat ggcgtgtcta cgcattcctc ttttatggcg tagtccgcgg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300

241 taagcggtaa gtccgacgcg ttgacaaccc ttcccgctag ccacgcccgg agaagcgata 300

.......... .......... .......... lacZ_a .......... ..........

.......... .......... .......... .......... .......... ..........

301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360

301 atgcggtcga ccgctttccc cctacacgac gttccgctaa ttcaacccat tgcggtccca 360

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccag tgaattaatt cgttgcagga 420

361 aaagggtcag tgctgcaaca ttttgctgcc ggtcacggtc acttaattaa gcaacgtcct 420

....... lacZ_a M13_pUC_f M13_f20 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc 480

421 gtcctacacc acgggctaca ctgatcgaga aacgacgtcc ggcaggatag gagaccaagg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt 540

481 ctattctctg ggtcttgagg ccggggggtg gcgggtggcg gtgggggtat gtatacacca 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagtttt gcatcccata 600

541 tgcgttcatt ctcacggacg cgtacggggt acacggggtg gttctcaaaa cgtagggtat 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac 660

601 gttcaggggt ttcacctctt ggcttggtta agaagcgccc gtcttgtttt cgaagacgtg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag 720

661 tgcagaggtg agcttaaacc tcggccggcc gcacacgttt tctccactta gcttgctttc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... MscI(771)

721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc 780

721 tgggcacaca tttcggcgca aaggttttac atattttggc tctcgtagac cggttacacg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggggatc 840

781 tagtcaacac cagtcgtcgt tttagttcac ttagtagagt cacgttgatt tcccccctag 840

.......... .......... .......... .......... Metal_pri ..........

..... KpnI(853),SpeI(856) ..... .......... AflII(881) ..........

841 tagatcgggg tacctactag tccagtgtgg tggaattgcc cttaagggca attctgcaga 900

841 atctagcccc atggatgatc aggtcacacc accttaacgg gaattcccgt taagacgtct 900

.......... .......... .......... .......... .......... ..........

EcoRV(901) NotI(916) XhoI(922) .......... .......... ..........

901 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat 960

901 ataggtcgtg tcaccgccgg cgagctcaga tctcccgggc gccaagcttc cattcggata 960

.......... .......... .......... .......... .......... V5

.......... .......... .......... AgeI(995) .......... ..........

961 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg 1020

961 gggattggga gaggagccag agctaagatg cgcatggcca gtagtagtgg tagtggtaac 1020

.......... V5 .......... .......... 6xHis ..........

.......... .......... .......... .......... .......... ..........

1021 agtttaaacc cgctgatcag cctcgactgt gccttctaag atccagacat gataagatac 1080

1021 tcaaatttgg gcgactagtc ggagctgaca cggaagattc taggtctgta ctattctatg 1080

.......... .......... BGH_r .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

1081 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa 1140

1081 taactactca aacctgtttg gtgttgatct tacgtcactt tttttacgaa ataaacactt 1140

......... EBV_r SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... HpaI(1194)

1141 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac 1200

1141 taaacactac gataacgaaa taaacattgg taatattcga cgttatttgt tcaattgttg 1200

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc 1260

1201 ttgttaacgt aagtaaaata caaagtccaa gtccccctcc acaccctcca aaaaatttcg 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... SalI(1302) ..........

1261 aagtaaaacc tctacaaatg tggtatggct gattatgatc agtcgacctg caggcatgca 1320

1261 ttcattttgg agatgtttac accataccga ctaatactag tcagctggac gtccgtacgt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 1380

1321 tcgaaccgca ttagtaccag tatcgacaaa ggacacactt taacaatagg cgagtgttaa 1380

.......... .......... M13_r .......... M13_pUC_r ..........

.......... .......... .......... .......... .......... ..........

1381 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 1440

1381 ggtgtgttgt atgctcggcc ttcgtatttc acatttcgga ccccacggat tactcactcg 1440

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 1500

1441 attgagtgta attaacgcaa cgcgagtgac gggcgaaagg tcagcccttt ggacagcacg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 1560

1501 gtcgacgtaa ttacttagcc ggttgcgcgc ccctctccgc caaacgcata acccgcgaga 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 1620

1561 aggcgaagga gcgagtgact gagcgacgcg agccagcaag ccgacgccgc tcgccatagt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 1680

1621 cgagtgagtt tccgccatta tgccaatagg tgtcttagtc ccctattgcg tcctttcttg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 1740

1681 tacactcgtt ttccggtcgt tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa 1740

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1741 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 1800

1741 aaggtatccg aggcgggggg actgctcgta gtgtttttag ctgcgagttc agtctccacc 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 1860

1801 gctttgggct gtcctgatat ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 1920

1861 agaggacaag gctgggacgg cgaatggcct atggacaggc ggaaagaggg aagcccttcg 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 1980

1921 caccgcgaaa gagtatcgag tgcgacatcc atagagtcaa gccacatcca gcaagcgagg 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 2040

1981 ttcgacccga cacacgtgct tggggggcaa gtcgggctgg cgacgcggaa taggccattg 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 2100

2041 atagcagaac tcaggttggg ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 2160

2101 ttgtcctaat cgtctcgctc catacatccg ccacgatgtc tcaagaactt caccaccgga 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 2220

2161 ttgatgccga tgtgatcttc ctgtcataaa ccatagacgc gagacgactt cggtcaatgg 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 2280

2221 aagccttttt ctcaaccatc gagaactagg ccgtttgttt ggtggcgacc atcgccacca 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 2340

2281 aaaaaacaaa cgttcgtcgt ctaatgcgcg tctttttttc ctagagttct tctaggaaac 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 2400

2341 tagaaaagat gccccagact gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 2460

2401 tactctaata gtttttccta gaagtggatc taggaaaatt taatttttac ttcaaaattt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 2520

2461 agttagattt catatatact catttgaacc agactgtcaa tggttacgaa ttagtcactc 2520

.......... .......... .......... .... Orf frame 3 Ampicillin ....

.......... .......... .......... .......... .......... ..........

2521 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 2580

2521 cgtggataga gtcgctagac agataaagca agtaggtatc aacggactga ggggcagcac 2580

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 2640

2581 atctattgat gctatgccct cccgaatggt agaccggggt cacgacgtta ctatggcgct 2640

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 2700

2641 ctgggtgcga gtggccgagg tctaaatagt cgttatttgg tcggtcggcc ttcccggctc 2700

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 2760

2701 gcgtcttcac caggacgttg aaataggcgg aggtaggtca gataattaac aacggccctt 2760

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 2820

2761 cgatctcatt catcaagcgg tcaattatca aacgcgttgc aacaacggta acgatgtccg 2820

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 2880

2821 tagcaccaca gtgcgagcag caaaccatac cgaagtaagt cgaggccaag ggttgctagt 2880

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 2940

2881 tccgctcaat gtactagggg gtacaacacg ttttttcgcc aatcgaggaa gccaggaggc 2940

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 3000

2941 tagcaacagt cttcattcaa ccggcgtcac aatagtgagt accaataccg tcgtgacgta 3000

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 3060

3001 ttaagagaat gacagtacgg taggcattct acgaaaagac actgaccact catgagttgg 3060

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 3120

3061 ttcagtaaga ctcttatcac atacgccgct ggctcaacga gaacgggccg cagttatgcc 3120

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 3180

3121 ctattatggc gcggtgtatc gtcttgaaat tttcacgagt agtaaccttt tgcaagaagc 3180

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 3240

3181 cccgcttttg agagttccta gaatggcgac aactctaggt caagctacat tgggtgagca 3240

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 3300

3241 cgtgggttga ctagaagtcg tagaaaatga aagtggtcgc aaagacccac tcgtttttgt 3300

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 3360

3301 ccttccgttt tacggcgttt tttcccttat tcccgctgtg cctttacaac ttatgagtat 3360

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 3420

3361 gagaaggaaa aagttataat aacttcgtaa atagtcccaa taacagagta ctcgcctatg 3420

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

3421 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 3480

3421 tataaactta cataaatctt tttatttgtt tatccccaag gcgcgtgtaa aggggctttt 3480

AmpR_pro .......... .......... .......... .......... ..........

......... AatII(3494) ......... .......... .......... ..........

3481 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 3540

3481 cacggtggac tgcagattct ttggtaataa tagtactgta attggatatt tttatccgca 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 atcacgaggc cctttcgt.. .......... .......... .......... .......... 3558

3541 tagtgctccg ggaaagca.. .......... .......... .......... .......... 3558

.......... .......... .......... .......... .......... ..........