Sequence of pMT_V5-His

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Sequence of pMT_V5-His_A

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Sequence of pMT/V5-His A : 3538 BP; 903 A; 885 C; 882 G; 868 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60

1 agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc ctctgccagt 60

.......... .......... ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120

61 gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180

121 aaccgcccac agccccgacc gaattgatac gccgtagtct cgtctaacat gactctcacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NarI(236)

181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240

181 tggtatacgc cacactttat ggcgtgtcta cgcattcctc ttttatggcg tagtccgcgg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300

241 taagcggtaa gtccgacgcg ttgacaaccc ttcccgctag ccacgcccgg agaagcgata 300

.......... .......... .......... lacZ_a .......... ..........

.......... .......... .......... .......... .......... ..........

301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360

301 atgcggtcga ccgctttccc cctacacgac gttccgctaa ttcaacccat tgcggtccca 360

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccag tgaattaatt cgttgcagga 420

361 aaagggtcag tgctgcaaca ttttgctgcc ggtcacggtc acttaattaa gcaacgtcct 420

....... lacZ_a M13_pUC_f M13_f20 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc 480

421 gtcctacacc acgggctaca ctgatcgaga aacgacgtcc ggcaggatag gagaccaagg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt 540

481 ctattctctg ggtcttgagg ccggggggtg gcgggtggcg gtgggggtat gtatacacca 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagtttt gcatcccata 600

541 tgcgttcatt ctcacggacg cgtacggggt acacggggtg gttctcaaaa cgtagggtat 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac 660

601 gttcaggggt ttcacctctt ggcttggtta agaagcgccc gtcttgtttt cgaagacgtg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag 720

661 tgcagaggtg agcttaaacc tcggccggcc gcacacgttt tctccactta gcttgctttc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... MscI(771)

721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc 780

721 tgggcacaca tttcggcgca aaggttttac atattttggc tctcgtagac cggttacacg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggggatc 840

781 tagtcaacac cagtcgtcgt tttagttcac ttagtagagt cacgttgatt tcccccctag 840

.......... .......... .......... .......... Metal_pri ..........

..... KpnI(853),SpeI(856) ..... EcoRI(873) EcoRV(885) ..........

841 tagatcgggg tacctactag tccagtgtgg tggaattctg cagatatcca gcacagtggc 900

841 atctagcccc atggatgatc aggtcacacc accttaagac gtctataggt cgtgtcaccg 900

.......... .......... .......... .......... .......... ..........

XhoI(906) .......... .......... .......... .......... ..........

901 ggccgctcga gtctagaggg cccttcgaag gtaagcctat ccctaaccct ctcctcggtc 960

901 ccggcgagct cagatctccc gggaagcttc cattcggata gggattggga gaggagccag 960

.......... .......... .......... .......... V5 ..........

.......... AgeI(975) .......... .......... .......... ..........

961 tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag 1020

961 agctaagatg cgcatggcca gtagtagtgg tagtggtaac tcaaatttgg gcgactagtc 1020

V5 .......... 6xHis .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cctcgactgt gccttctaag atccagacat gataagatac attgatgagt ttggacaaac 1080

1021 ggagctgaca cggaagattc taggtctgta ctattctatg taactactca aacctgtttg 1080

BGH_r .......... .......... ......... SV40_PA_term EBV_r

.......... .......... .......... .......... .......... ..........

1081 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt 1140

1081 gtgttgatct tacgtcactt tttttacgaa ataaacactt taaacactac gataacgaaa 1140

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... HpaI(1174) .......... ..........

1141 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat 1200

1141 taaacattgg taatattcga cgttatttgt tcaattgttg ttgttaacgt aagtaaaata 1200

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg 1260

1201 caaagtccaa gtccccctcc acaccctcca aaaaatttcg ttcattttgg agatgtttac 1260

.......... .......... .......... .......... .......... ..........

.......... .......... SalI(1282) .......... .......... ..........

1261 tggtatggct gattatgatc agtcgacctg caggcatgca agcttggcgt aatcatggtc 1320

1261 accataccga ctaatactag tcagctggac gtccgtacgt tcgaaccgca ttagtaccag 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 1380

1321 tatcgacaaa ggacacactt taacaatagg cgagtgttaa ggtgtgttgt atgctcggcc 1380

M13_r ......... M13_pUC_rev ......... .......... lac_pro

.......... .......... .......... .......... .......... ..........

1381 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 1440

1381 ttcgtatttc acatttcgga ccccacggat tactcactcg attgagtgta attaacgcaa 1440

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 1500

1441 cgcgagtgac gggcgaaagg tcagcccttt ggacagcacg gtcgacgtaa ttacttagcc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 1560

1501 ggttgcgcgc ccctctccgc caaacgcata acccgcgaga aggcgaagga gcgagtgact 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 1620

1561 gagcgacgcg agccagcaag ccgacgccgc tcgccatagt cgagtgagtt tccgccatta 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 1680

1621 tgccaatagg tgtcttagtc ccctattgcg tcctttcttg tacactcgtt ttccggtcgt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 1740

1681 tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg 1740

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1741 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 1800

1741 actgctcgta gtgtttttag ctgcgagttc agtctccacc gctttgggct gtcctgatat 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 1860

1801 ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg agaggacaag gctgggacgg 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 1920

1861 cgaatggcct atggacaggc ggaaagaggg aagcccttcg caccgcgaaa gagtatcgag 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 1980

1921 tgcgacatcc atagagtcaa gccacatcca gcaagcgagg ttcgacccga cacacgtgct 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 2040

1981 tggggggcaa gtcgggctgg cgacgcggaa taggccattg atagcagaac tcaggttggg 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 2100

2041 ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 2160

2101 catacatccg ccacgatgtc tcaagaactt caccaccgga ttgatgccga tgtgatcttc 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 2220

2161 ctgtcataaa ccatagacgc gagacgactt cggtcaatgg aagccttttt ctcaaccatc 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 2280

2221 gagaactagg ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 2340

2281 ctaatgcgcg tctttttttc ctagagttct tctaggaaac tagaaaagat gccccagact 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 2400

2341 gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag tactctaata gtttttccta 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 2460

2401 gaagtggatc taggaaaatt taatttttac ttcaaaattt agttagattt catatatact 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 2520

2461 catttgaacc agactgtcaa tggttacgaa ttagtcactc cgtggataga gtcgctagac 2520

.......... .......... .... Orf frame 1 Ampicillin .... ..........

.......... .......... .......... .......... .......... ..........

2521 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 2580

2521 agataaagca agtaggtatc aacggactga ggggcagcac atctattgat gctatgccct 2580

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 2640

2581 cccgaatggt agaccggggt cacgacgtta ctatggcgct ctgggtgcga gtggccgagg 2640

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 2700

2641 tctaaatagt cgttatttgg tcggtcggcc ttcccggctc gcgtcttcac caggacgttg 2700

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 2760

2701 aaataggcgg aggtaggtca gataattaac aacggccctt cgatctcatt catcaagcgg 2760

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 2820

2761 tcaattatca aacgcgttgc aacaacggta acgatgtccg tagcaccaca gtgcgagcag 2820

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 2880

2821 caaaccatac cgaagtaagt cgaggccaag ggttgctagt tccgctcaat gtactagggg 2880

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 2940

2881 gtacaacacg ttttttcgcc aatcgaggaa gccaggaggc tagcaacagt cttcattcaa 2940

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 3000

2941 ccggcgtcac aatagtgagt accaataccg tcgtgacgta ttaagagaat gacagtacgg 3000

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 3060

3001 taggcattct acgaaaagac actgaccact catgagttgg ttcagtaaga ctcttatcac 3060

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 3120

3061 atacgccgct ggctcaacga gaacgggccg cagttatgcc ctattatggc gcggtgtatc 3120

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 3180

3121 gtcttgaaat tttcacgagt agtaaccttt tgcaagaagc cccgcttttg agagttccta 3180

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 3240

3181 gaatggcgac aactctaggt caagctacat tgggtgagca cgtgggttga ctagaagtcg 3240

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 3300

3241 tagaaaatga aagtggtcgc aaagacccac tcgtttttgt ccttccgttt tacggcgttt 3300

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 3360

3301 tttcccttat tcccgctgtg cctttacaac ttatgagtat gagaaggaaa aagttataat 3360

.... Orf frame 1 Ampicillin .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 3420

3361 aacttcgtaa atagtcccaa taacagagta ctcgcctatg tataaactta cataaatctt 3420

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... ......... AatII(3474)

3421 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 3480

3421 tttatttgtt tatccccaag gcgcgtgtaa aggggctttt cacggtggac tgcagattct 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgt.. 3538

3481 ttggtaataa tagtactgta attggatatt tttatccgca tagtgctccg ggaaagca.. 3538

.......... .......... .......... .......... .......... ..........