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pNrl-Cre
Gene/insert name: Nrl promoter-Cre
Alternative names: Nrl
Insert size (bp): 4308
Gene/insert aliases: Nrl, AW492574, D14H14S46E
Species of gene(s): M. musculus (mouse)
Fusion proteins or tags: Cre
Terminal: C terminal on backbone
Vector backbone: na (Search Vector Database)
Type of vector: Mammalian expression,Cre/Lox
Backbone size (bp): 7369
Cloning site 5': SalI
Site destroyed during cloning: No
Cloning site 3': NotI
Site destroyed during cloning: No
5' Sequencing primer: na (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: The coding sequence of Cre was amplified by PCR using pxCANCre (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo) as a template.
Plasmid Provided In: DH5a
Principal Investigator: Connie Cepko
Comments: Mouse Nrl promoter was isolated by genomic PCR using B6 mouse genomic DNA as a template. The Nrl promoter-Cre is specific for rod photorecetor cells in the retina. Cre has a Myc-tag at its C-terminus.
Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T et al. (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. Pubmed)