Sequence of pNrl-Cre

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Sequence of pNrl-Cre

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Sequence of pNrl-Cre : 7369 BP; 1899 A; 1694 C; 1817 G; 1959 T; 0 other;

SalI(1) .......... .......... .......... .......... ..........

1 gtcgacttac atgtacatac ttcataatgg tctagaaggg cttattcaaa tttggcgttt 60

1 cagctgaatg tacatgtatg aagtattacc agatcttccc gaataagttt aaaccgcaaa 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 actcagagtc ctcggggctc ttatccggga tgtggtgatg atgtgaggag aggcaactct 120

61 tgagtctcag gagccccgag aataggccct acaccactac tacactcctc tccgttgaga 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 caaactagga ccatcactcg gggcttgtgt atcagtagtg aggtccagca atggaaggga 180

121 gtttgatcct ggtagtgagc cccgaacaca tagtcatcac tccaggtcgt taccttccct 180

.......... .......... .......... .......... .......... ..........

SmaI(186) .......... .......... .......... SacI(227) ..........

181 aatcccggga aaaaagtcta tgtgatccct aggagcaggt ctgagctctt tgtgcagaaa 240

181 ttagggccct tttttcagat acactaggga tcctcgtcca gactcgagaa acacgtcttt 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 atttctggat acctggagta tgattctcag ggaccagggg tctgggtcag agaagggtgg 300

241 taaagaccta tggacctcat actaagagtc cctggtcccc agacccagtc tcttcccacc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ctgtctcaca tcatggggta cattcagcta tagaaagacc agcatgaacc aggcagtggt 360

301 gacagagtgt agtaccccat gtaagtcgat atctttctgg tcgtacttgg tccgtcacca 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acctggcctg aaccaggact ggctcagctt gcttgagctt atttggcccg actcccttca 420

361 tggaccggac ttggtcctga ccgagtcgaa cgaactcgaa taaaccgggc tgagggaagt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctccttagca gcttagctct cccacctctc tcctgagtcc tccagtaaac aagaggatca 480

421 gaggaatcgt cgaatcgaga gggtggagag aggactcagg aggtcatttg ttctcctagt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggacagatat gacagctgcc tggtggggca agtgtctggt caggtcaaca gcaggcatga 540

481 cctgtctata ctgtcgacgg accaccccgt tcacagacca gtccagttgt cgtccgtact 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 agggggctac tacactaaat gctgccaacc tttttccttt agggaagttc taggatctct 600

541 tcccccgatg atgtgattta cgacggttgg aaaaaggaaa tcccttcaag atcctagaga 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ttcccggata tccactgtaa aacctcattc cactggcatg tatccctatt cctttcagca 660

601 aagggcctat aggtgacatt ttggagtaag gtgaccgtac atagggataa ggaaagtcgt 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tccctattgc ccttttgtgg atggttcctg gccttacatt ttccgcttct tttaagaaaa 720

661 agggataacg ggaaaacacc taccaaggac cggaatgtaa aaggcgaaga aaattctttt 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 taagcaacag gtcttggtga agagtaacat ttccattcga atagtgactc tatttcataa 780

721 attcgttgtc cagaaccact tctcattgta aaggtaagct tatcactgag ataaagtatt 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 accaaacgaa acgaaacgaa acgaaacgaa acgaaacaaa acaaaacaaa acaaaacacc 840

781 tggtttgctt tgctttgctt tgctttgctt tgctttgttt tgttttgttt tgttttgtgg 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aagagacctg ggcatgggag agttgtgata tctgacctaa gccttctaat gtatgtagag 900

841 ttctctggac ccgtaccctc tcaacactat agactggatt cggaagatta catacatctc 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gaagcttgga ttccaaatgt cctccaaagc tgggcatggt ggtgcgtgcc tttacgccct 960

901 cttcgaacct aaggtttaca ggaggtttcg acccgtacca ccacgcacgg aaatgcggga 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gtactaggaa ggcacaagca ggtagatcta tgggttcaag gtcaatctgg tctacaaaga 1020

961 catgatcctt ccgtgttcgt ccatctagat acccaagttc cagttagacc agatgtttct 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gagttctagg acggctaggg ctacacagag aaaccctgtt ttgaaaacca aaccaaacca 1080

1021 ctcaagatcc tgccgatccc gatgtgtctc tttgggacaa aacttttggt ttggtttggt 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aaccaatgtc ctccaaaggc atttgagttc aaaacttgtc attattggaa ggtggtgaaa 1140

1081 ttggttacag gaggtttccg taaactcaag ttttgaacag taataacctt ccaccacttt 1140

.......... .......... .......... .......... .......... ..........

.......... ApaI(1158) .......... .......... .......... ..........

1141 ttttcaagag gtggggccct gtgggaagtc ttctggtctt tgggttttcc ctgaaggaag 1200

1141 aaaagttctc caccccggga cacccttcag aagaccagaa acccaaaagg gacttccttc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 caaaggccta acccctccct ggtcatcggt tggtaccctt gagggaagca ggagtcctat 1260

1201 gtttccggat tggggaggga ccagtagcca accatgggaa ctcccttcgt cctcaggata 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccacttccct tcccttttct tttttttttt ttttgtccca gtgataaggt gagtagcttt 1320

1261 ggtgaaggga agggaaaaga aaaaaaaaaa aaaacagggt cactattcca ctcatcgaaa 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cagttgtgtc ctcgccataa cacttgactc actacaggca atacatgatg ggaccaactg 1380

1321 gtcaacacag gagcggtatt gtgaactgag tgatgtccgt tatgtactac cctggttgac 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ctcactggct aggctctcca aggcagcaaa ccaaagcaaa gcgctttccg attaagtcaa 1440

1381 gagtgaccga tccgagaggt tccgtcgttt ggtttcgttt cgcgaaaggc taattcagtt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttacttcagg tatgtattgg agcaattatg ttagtgttag tcggtttttg ttattgtgac 1500

1441 aatgaagtcc atacataacc tcgttaatac aatcacaatc agccaaaaac aataacactg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaagtgcccg agaaagatca aaggagggga gccttctttg ggctaatggc ttcagtttca 1560

1501 tttcacgggc tctttctagt ttcctcccct cggaagaaac ccgattaccg aagtcaaagt 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gctgatggtt gtgggtgtcc tattgctttg gtccaaaatc taacatcacg atggaaaatg 1620

1561 cgactaccaa cacccacagg ataacgaaac caggttttag attgtagtgc taccttttac 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tatagtgaag aagtaaagag agaaaagaga gaggaagagg gggaagtgga ggtgggggag 1680

1621 atatcacttc ttcatttctc tcttttctct ctccttctcc cccttcacct ccaccccctc 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aaggagggat ggagggacac agacaaaatg caccaatgaa ctaaggagaa gatacaccat 1740

1681 ttcctcccta cctccctgtg tctgttttac gtggttactt gattcctctt ctatgtggta 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcaaacgcat gttttttcta gttaggccaa ttcatactga cagccaattc agtatggatt 1800

1741 agtttgcgta caaaaaagat caatccggtt aagtatgact gtcggttaag tcatacctaa 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cttcagctgg ttagttatgg ataagtttaa cctctaataa tatctcagtg aaaactagct 1860

1801 gaagtcgacc aatcaatacc tattcaaatt ggagattatt atagagtcac ttttgatcga 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tttaaaatat accggaacca tagcagtgat agaaaactaa tgtagggtgg acacacacac 1920

1861 aaattttata tggccttggt atcgtcacta tcttttgatt acatcccacc tgtgtgtgtg 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acacacacac acacaaacac acactttgag actgggttga aggtaggcat ctgtggacca 1980

1921 tgtgtgtgtg tgtgtttgtg tgtgaaactc tgacccaact tccatccgta gacacctggt 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gtgggtctca aacattagtg tgtttcagag ctgttggaag ggtttattaa acagcagctg 2040

1981 cacccagagt ttgtaatcac acaaagtctc gacaaccttc ccaaataatt tgtcgtcgac 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tctataacct acttactgag tttctggttc tgtgagtttg aggtaaagca aaagaatttg 2100

2041 agatattgga tgaatgactc aaagaccaag acactcaaac tccatttcgt tttcttaaac 2100

.......... .......... .......... .......... .......... ..........

.......... ......... AflII(2128) ......... .......... ..........

2101 tttcttcaag ttcccaaggg gtgatttctt aagactgtgg aagccattgt tccaacaggc 2160

2101 aaagaagttc aagggttccc cactaaagaa ttctgacacc ttcggtaaca aggttgtccg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agggaatacc ctttaacttc tggagtttct tttatcacag cacctattta aacagcttca 2220

2161 tcccttatgg gaaattgaag acctcaaaga aaatagtgtc gtggataaat ttgtcgaagt 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ggcaggttta aacatctcct tggctaagtc ctactatccc tgcttcggcc cctactaggg 2280

2221 ccgtccaaat ttgtagagga accgattcag gatgataggg acgaagccgg ggatgatccc 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggtgacagct ctaagaggcg ttaggcgatt aggctgaaaa tgtaggtcac accccagccg 2340

2281 ccactgtcga gattctccgc aatccgctaa tccgactttt acatccagtg tggggtcggc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tctgggaatg agcgagagag gagaagaggg actgagacac cgcacgggga aaaggtgaga 2400

2341 agacccttac tcgctctctc ctcttctccc tgactctgtg gcgtgcccct tttccactct 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggaagctgag ggctcatgag ggcattcaca gaagtgtggt ggtgattgga tttcagcttc 2460

2401 ccttcgactc ccgagtactc ccgtaagtgt cttcacacca ccactaacct aaagtcgaag 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttttagtctc cagatgaaag accagagttg ggatctccgc tttttctatc tcttctctcc 2520

2461 aaaatcagag gtctactttc tggtctcaac cctagaggcg aaaaagatag agaagagagg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agctgggctg cttgtcttcg ggatgggcta ttaagagcga gtttcttcat ggaagctcat 2580

2521 tcgacccgac gaacagaagc cctacccgat aattctcgct caaagaagta ccttcgagta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggcttatctt tctaatacag tgactctgtg aggaataaga cgagtcaggg tgaaggtagg 2640

2581 ccgaatagaa agattatgtc actgagacac tccttattct gctcagtccc acttccatcc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcaggggctg aaatgtgagg atgcttgaaa agaagcagca gccccaacaa gctggctgag 2700

2641 cgtccccgac tttacactcc tacgaacttt tcttcgtcgt cggggttgtt cgaccgactc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gatgggctgg gaacgcgtat atgtctcctt gacacggagt cttaaaagag gttttgggta 2760

2701 ctacccgacc cttgcgcata tacagaggaa ctgtgcctca gaattttctc caaaacccat 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gtatgtctgg actcttacca ctctaagctt tgagtgattg ggctgagaat tgtgtttcca 2820

2761 catacagacc tgagaatggt gagattcgaa actcactaac ccgactctta acacaaaggt 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tggaggggtg gtgtttgggg tcaacatggc tctctatatt ggaaaccaag ctcacaacat 2880

2821 acctccccac cacaaacccc agttgtaccg agagatataa cctttggttc gagtgttgta 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aagcaaggtc tgatatagga agcttctctg gggaagatgc cgtttgtttt tttccttttt 2940

2881 ttcgttccag actatatcct tcgaagagac cccttctacg gcaaacaaaa aaaggaaaaa 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggagaactga tagcacctgt ctttcactgg cttctgagtc cctgccccag gccaatgaga 3000

2941 cctcttgact atcgtggaca gaaagtgacc gaagactcag ggacggggtc cggttactct 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... XhoI(3035) .......... ..........

3001 tatgcaaata agcccttcat cttattggat gaggctcgag gaccacagat gatctcaggg 3060

3001 atacgtttat tcgggaagta gaataaccta ctccgagctc ctggtgtcta ctagagtccc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aaccagtcct ttaagagtgt cctctgggct ctgtgcccac agcccccttg ttctgaatac 3120

3061 ttggtcagga aattctcaca ggagacccga gacacgggtg tcgggggaac aagacttatg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agggacgaca ccagcccctg ctctatggag tatttagtct ccagggaagc tgtgcctttc 3180

3121 tccctgctgt ggtcggggac gagatacctc ataaatcaga ggtcccttcg acacggaaag 3180

.......... .......... .......... .......... .......... ..........

.......... .......... ......... EcoRI(3217) ......... ..........

3181 tggttctgac agtgactacg tcatctctgc cattacgaat tctgagccgc caccatggcc 3240

3181 accaagactg tcactgatgc agtagagacg gtaatgctta agactcggcg gtggtaccgg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aatttactga ccgtacacca aaatttgcct gcattaccgg tcgatgcaac gagtgatgag 3300

3241 ttaaatgact ggcatgtggt tttaaacgga cgtaatggcc agctacgttg ctcactactc 3300

.......... .......... ORF 1 CRE_NLS .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gttcgcaaga acctgatgga catgttcagg gatcgccagg cgttttctga gcatacctgg 3360

3301 caagcgttct tggactacct gtacaagtcc ctagcggtcc gcaaaagact cgtatggacc 3360

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa 3420

3361 ttttacgaag acaggcaaac ggccagcacc cgccgtacca cgttcaactt attggccttt 3420

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... NruI(3449) .......... .......... ..........

3421 tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca ggcgcgcggt 3480

3421 accaaagggc gtcttggact tctacaagcg ctaatagaag atatagaagt ccgcgcgcca 3480

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca tcgtcggtcc 3540

3481 gaccgtcatt tttgataggt cgttgtaaac ccggtcgatt tgtacgaagt agcagccagg 3540

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa 3600

3541 cccgacggtg ctggttcact gtcgttacga caaagtgacc aatacgccgc ctaggctttt 3600

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc 3660

3601 cttttgcaac tacggccact tgcacgtttt gtccgagatc gcaagcttgc gtgactaaag 3660

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gaccaggttc gttcactcat ggaaaatagc gatcgctgcc aggatatacg taatctggca 3720

3661 ctggtccaag caagtgagta ccttttatcg ctagcgacgg tcctatatgc attagaccgt 3720

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag gatcagggtt 3780

3721 aaagacccct aacgaatatt gtgggacaat gcatatcggc tttaacggtc ctagtcccaa 3780

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag aacgaaaacg 3840

3781 tttctataga gtgcatgact gccaccctct tacaattagg tataaccgtc ttgcttttgc 3840

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa actggtcgag 3900

3841 gaccaatcgt ggcgtccaca tctcttccgt gaatcggacc cccattgatt tgaccagctc 3900

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgggtc 3960

3901 gctacctaaa ggcagagacc acatcgacta ctaggcttat tgatggacaa aacggcccag 3960

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa 4020

3961 tcttttttac cacaacggcg cggtagacgg tggtcggtcg atagttgagc gcgggacctt 4020

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... ClaI(4041) .......... .......... ..........

4021 gggatttttg aagcaactca tcgattgatt tacggcgcta aggatgactc tggtcagaga 4080

4021 ccctaaaaac ttcgttgagt agctaactaa atgccgcgat tcctactgag accagtctct 4080

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct 4140

4081 atggaccgga ccagacctgt gtcacgggca cagcctcggc gcgctctata ccgggcgcga 4140

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggagtttcaa taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg 4200

4141 cctcaaagtt atggcctcta gtacgttcga ccaccgacct ggttacattt ataacagtac 4200

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc 4260

4201 ttgatatagg cattggacct atcactttgt ccccgttacc acgcggacga ccttctaccg 4260

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .... NotI(4309),EagI(4309) ....

4261 gatggaccgg tggaacaaaa acttatttct gaagaagatc tgtgatagcg gccgcactcc 4320

4261 ctacctggcc accttgtttt tgaataaaga cttcttctag acactatcgc cggcgtgagg 4320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa 4380

4321 agtccacgtc cgacggatag tcttccacca ccgaccacac cggttacggg accgagtgtt 4380

.......... .......... ....... rb_glob_PA_term ....... ..........

.......... .......... .......... .......... .......... ..........

4381 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga 4440

4381 tatggtgact ctagaaaaag ggagacggtt tttaataccc ctgtagtact tcggggaact 4440

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt 4500

4441 cgtagactga agaccgatta tttcctttaa ataaaagtaa cgttatcaca caaccttaaa 4500

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga 4560

4501 aaacacagag agtgagcctt cctgtatacc ctcccgttta gtaaattttg tagtcttact 4560

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtatttggtt tagagtttgg caacatatgc catatgctgg ctgccatgaa caaaggtggc 4620

4561 cataaaccaa atctcaaacc gttgtatacg gtatacgacc gacggtactt gtttccaccg 4620

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa 4680

4621 atatttctcc agtagtcata tactttgtcg ggggacgaca ggtaaggaat aaggtatctt 4680

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa 4740

4681 ttcggaactg aactccaatc taaaaaaaat ataaaacaaa acacaataaa aaaagaaatt 4740

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac 4800

4741 gtagggattt taaaaggaat gtacaaaatg atcggtctaa aaaggaggag aggactgatg 4800

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... PstI(4849) ..........

4801 tcccagtcat agctgtccct cttctcttat gaagatccct cgacctgcag cccaagcttg 4860

4801 agggtcagta tcgacaggga gaagagaata cttctaggga gctggacgtc gggttcgaac 4860

....... rb_glob_PA_term ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 4920

4861 cgcattagta ccagtatcga caaaggacac actttaacaa taggcgagtg ttaaggtgtg 4920

.......... M13_r M13_pUC_r .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 4980

4921 ttgtatgctc ggccttcgta tttcacattt cggaccccac ggattactca ctcgattgag 4980

.......... lac .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg 5040

4981 tgtaattaac gcaacgcgag tgacgggcga aaggtcagcc ctttggacag cacggtcgcc 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 5100

5041 taggcgtaga gttaatcagt cgttggtatc agggcgggga ttgaggcggg tagggcgggg 5100

.......... .......... .......... SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5101 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 5160

5101 attgaggcgg gtcaaggcgg gtaagaggcg gggtaccgac tgattaaaaa aaataaatac 5160

.......... ......... SV40_pro SV40_origin ......... ..........

.......... .......... .......... .......... .......... ..........

5161 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 5220

5161 gtctccggct ccggcggagc cggagactcg ataaggtctt catcactcct ccgaaaaaac 5220

SV40pro_F .......... SV40_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata 5280

5221 ctccggatcc gaaaacgttt ttcgattgaa caaataacgt cgaatattac caatgtttat 5280

SV40_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 5340

5281 ttcgttatcg tagtgtttaa agtgtttatt tcgtaaaaaa agtgacgtaa gatcaacacc 5340

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc 5400

5341 aaacaggttt gagtagttac atagaatagt acagacctag gcgacgtaat tacttagccg 5400

EBV_r SV40_PA_term ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 5460

5401 gttgcgcgcc cctctccgcc aaacgcataa cccgcgagaa ggcgaaggag cgagtgactg 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 5520

5461 agcgacgcga gccagcaagc cgacgccgct cgccatagtc gagtgagttt ccgccattat 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 5580

5521 gccaataggt gtcttagtcc cctattgcgt cctttcttgt acactcgttt tccggtcgtt 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 5640

5581 ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa aggtatccga ggcgggggga 5640

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5641 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 5700

5641 ctgctcgtag tgtttttagc tgcgagttca gtctccaccg ctttgggctg tcctgatatt 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 5760

5701 tctatggtcc gcaaaggggg accttcgagg gagcacgcga gaggacaagg ctgggacggc 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca 5820

5761 gaatggccta tggacaggcg gaaagaggga agcccttcgc accgcgaaag agttacgagt 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 5880

5821 gcgacatcca tagagtcaag ccacatccag caagcgaggt tcgacccgac acacgtgctt 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 5940

5881 ggggggcaag tcgggctggc gacgcggaat aggccattga tagcagaact caggttgggc 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 6000

5941 cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 6060

6001 atacatccgc cacgatgtct caagaacttc accaccggat tgatgccgat gtgatcttcc 6060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6061 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 6120

6061 tgtcataaac catagacgcg agacgacttc ggtcaatgga agcctttttc tcaaccatcg 6120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 6180

6121 agaactaggc cgtttgtttg gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc 6180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6181 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 6240

6181 taatgcgcgt ctttttttcc tagagttctt ctaggaaact agaaaagatg ccccagactg 6240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 6300

6241 cgagtcacct tgcttttgag tgcaattccc taaaaccagt actctaatag tttttcctag 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 6360

6301 aagtggatct aggaaaattt aatttttact tcaaaattta gttagatttc atatatactc 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 6420

6361 atttgaacca gactgtcaat ggttacgaat tagtcactcc gtggatagag tcgctagaca 6420

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

6421 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 6480

6421 gataaagcaa gtaggtatca acggactgag gggcagcaca tctattgatg ctatgccctc 6480

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 6540

6481 ccgaatggta gaccggggtc acgacgttac tatggcgctc tgggtgcgag tggccgaggt 6540

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 6600

6541 ctaaatagtc gttatttggt cggtcggcct tcccggctcg cgtcttcacc aggacgttga 6600

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 6660

6601 aataggcgga ggtaggtcag ataattaaca acggcccttc gatctcattc atcaagcggt 6660

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... FspI(6673) .......... .......... .......... ..........

6661 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 6720

6661 caattatcaa acgcgttgca acaacggtaa cgatgtccgt agcaccacag tgcgagcagc 6720

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 6780

6721 aaaccatacc gaagtaagtc gaggccaagg gttgctagtt ccgctcaatg tactaggggg 6780

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 6840

6781 tacaacacgt tttttcgcca atcgaggaag ccaggaggct agcaacagtc ttcattcaac 6840

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 6900

6841 cggcgtcaca atagtgagta ccaataccgt cgtgacgtat taagagaatg acagtacggt 6900

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 6960

6901 aggcattcta cgaaaagaca ctgaccactc atgagttggt tcagtaagac tcttatcaca 6960

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 7020

6961 tacgccgctg gctcaacgag aacgggccgc agttatgccc tattatggcg cggtgtatcg 7020

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 7080

7021 tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc ccgcttttga gagttcctag 7080

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 7140

7081 aatggcgaca actctaggtc aagctacatt gggtgagcac gtgggttgac tagaagtcgt 7140

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 7200

7141 agaaaatgaa agtggtcgca aagacccact cgtttttgtc cttccgtttt acggcgtttt 7200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 7260

7201 ttcccttatt cccgctgtgc ctttacaact tatgagtatg agaaggaaaa agttataata 7260

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 7320

7261 acttcgtaaa tagtcccaat aacagagtac tcgcctatgt ataaacttac ataaatcttt 7320

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

7321 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg. .......... 7369

7321 ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc acggtggac. .......... 7369

.......... .......... .......... .......... .......... ..........