MyMy+LOCUS pOBD-2 7167 bp DNA SYN 07-Sep-2007 DEFINITION pOBD-2 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7167 /organism="pOBD-2" /mol_type="other DNA" misc_feature 434..874 /label="Gal4_DBD" misc_feature 838..856 /label="Gal4_N_term_primer" misc_feature complement(1235..1258) /label="pJG4_5_rev_primer" terminator 1219..1542 /label="yADH2_terminator" misc_feature complement(1411..1435) /label="pHybLex_rev_primer" terminator 1356..1547 /label="yADH1_terminator" misc_feature 1814..2666 /label="CEN4" CDS complement(3376..4050) /label="TRP1" /gene="TRP1" /note="ORF frame 1" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK*" gene complement(3376..4050) /label="TRP1" /gene="TRP1" promoter 4293..4321 /label="AmpR_promoter" CDS 4363..5223 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 4363..5223 /label="Ampicillin" /gene="Ampicillin" rep_origin 5378..5997 /label="pBR322_origin" ORIGIN 1 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt 61 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt 121 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa 181 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt 241 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattga 301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca 421 agcctcctga aagATGAAGC TACTGTCTTC TATCGAACAA GCATGCGATA TTTGCCGACT 481 TAAAAAGCTC AAGTGCTCCA AAGAAAAACC GAAGTGCGCC AAGTGTCTGA AGAACAACTG 541 GGAGTGTCGC TACTCTCCCA AAACCAAAAG GTCTCCGCTG ACTAGGGCAC ATCTGACAGA 601 AGTGGAATCA AGGCTAGAAA GACTGGAACA GCTATTTCTA CTGATTTTTC CTCGAGAAGA 661 CCTTGACATG ATTTTGAAAA TGGATTCTTT ACAGGATATA AAAGCATTGT TAACAGGATT 721 ATTTGTACAA GATAATGTGA ATAAAGATGC CGTCACAGAT AGATTGGCTT CAGTGGAGAC 781 TGATATGCCT CTAACATTGA GACAGCATAG AATAAGTGCG ACATCATCAT CGGAAGAGAG 841 TAGTAACAAA GGTCAAAGAC AGTTGACTGT ATCGAACTAT CTATTCGATG ATGAAGATAC 901 CCCACCAAAC CCAAAAAAAG AGATCGAATT CCAGCTGACC ACCATGGCAA TTCCCGGGGA 961 TCCGTCGACC TGCAGAGATC TAtgaatcgt agatactgaa aaaccccgca agttcacttc 1021 aactgtgcat cgtgcaccat ctcaatttct ttcatttata catcgttttg ccttctttta 1081 tgtaactata ctcctctaag tttcaatctt ggccatgtaa cctctgatct atagaatttt 1141 ttaaatgact agaattaatg cccatctttt ttttggacct aaattcttca tgaaaatata 1201 ttacgagggc ttattcagaa gctttggact tcttcgccag aggtttggtc aagtctccaa 1261 tcaaggttgt cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa 1321 tcgttggtag atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt 1381 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg 1441 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc 1501 aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgccg agcaaatgcc 1561 tgcaaatcgc tccccatttc acccaattgt agatatgcta actccagcaa tgagttgatg 1621 aatctcggtg tgtattttat gtcctcagag gacaacacct gttgtaatcg ttcttccaca 1681 cggatcctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 1741 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc 1801 acaccgcata tatcgctggg ccattctcat gaagaatatc ttgaatttat tgtcatatta 1861 ctagttggtg tggaagtcca tatatcggtg atcaatatag tggttgacat gctggctagt 1921 caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca aatatcaaac 1981 ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa gaaaaaaatt 2041 actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg tctcaccata 2101 gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata catataatac 2161 acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa attggctagt 2221 gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg taaaagttca 2281 ctttagcata catatattac acgagccaga aattgtaact tttgcctaaa atcacaaatt 2341 gcaaaattta attgcttgca aaaggtcaca tgcttataat caactttttt aaaaatttaa 2401 aatacttttt tattttttat ttttaaacat aaatgaaata atttatttat tgtttatgat 2461 taccgaaaca taaaacctgc tcaagaaaaa gaaactgttt tgtccttgga aaaaaagcac 2521 tacctaggag cggccaaaat gccgaggctt tcatagctta aactctttac agaaaatagg 2581 cattatagat cagttcgagt tttcttattc ttccttccgg ttttatcgtc acagttttac 2641 agtaaataag tatcacctct tagagttcga tgataagctg tcaaacatga gaattaattc 2701 cacatgttaa aatagtgaag gagcatgttc ggcacacagt ggaccgaacg tggggtaagt 2761 gcactagggt ccggttaaac ggatctcgca ttgatgaggc aacgctaatt atcaacatat 2821 agattgttat ctatctgcat gaacacgaaa tctttacttg acgacttgag gctgatggtg 2881 tttatgcaaa gaaaccactg tgtttaatat gtgtcactgt ttgatattac tgtcagcgta 2941 gaagataata gtaaaagcgg ttaataagtg tatttgagat aagtgtgata aagtttttac 3001 agcgaaaaga cgataaatac aagaaaatga ttacgaggat acggagagag gtatgtacat 3061 gtgtatttat atactaagct gccggcggtt gtttgcaaga ccgagaaaag gctagcaaga 3121 atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt gattccattg 3181 cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa tcaatcaaaa 3241 agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta tgtttagatc 3301 ttttatgctt gcttttcaaa aggcttgcag gcaagtgcac aaacaatact taaataaata 3361 ctactcagta ataacctaTT TCTTAGCATT TTTGACGAAA TTTGCTATTT TGTTAGAGTC 3421 TTTTACACCA TTTGTCTCCA CACCTCCGCT TACATCAACA CCAATAACGC CATTTAATCT 3481 AAGCGCATCA CCAACATTTT CTGGCGTCAG TCCACCAGCT AACATAAAAT GTAAGCTCTC 3541 GGGGCTCTCT TGCCTTCCAA CCCAGTCAGA AATCGAGTTC CAATCCAAAA GTTCACCTGT 3601 CCCACCTGCT TCTGAATCAA ACAAGGGAAT AAACGAATGA GGTTTCTGTG AAGCTGCACT 3661 GAGTAGTATG TTGCAGTCTT TTGGAAATAC GAGTCTTTTA ATAACTGGCA AACCGAGGAA 3721 CTCTTGGTAT TCTTGCCACG ACTCATCTCC ATGCAGTTGG ACGATATCAA TGCCGTAATC 3781 ATTGACCAGA GCCAAAACAT CCTCCTTAGG TTGATTACGA AACACGCCAA CCAAGTATTT 3841 CGGAGTGCCT GAACTATTTT TATATGCTTT TACAAGACTT GAAATTTTCC TTGCAATAAC 3901 CGGGTCAATT GTTCTCTTTC TATTGGGCAC ACATATAATA CCCAGCAAGT CAGCATCGGA 3961 ATCTAGTGCA CATTCTGCGG CCTCTGTGCT CTGCAAGCCG CAAACTTTCA CCAATGGACC 4021 AGAACTACCT GTGAAATTAA TAACAGACAT actccaagct gcctttgtgt gcttaatcac 4081 gtatactcac gtgctcaata gtcaccaatg ccctccctct tggccctctc cttttctttt 4141 ttcgaccgaa ttaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt 4201 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc 4261 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 4321 taaccctgat aaatgcttca ataatattga aaaaggaaga gtATGAGTAT TCAACATTTC 4381 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA 4441 ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA 4501 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG 4561 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA 4621 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC 4681 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC 4741 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA 4801 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG 4861 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA 4921 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA 4981 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC 5041 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA 5101 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA 5161 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG 5221 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 5281 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 5341 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 5401 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 5461 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 5521 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 5581 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5641 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 5701 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 5761 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 5821 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 5881 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 5941 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 6001 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 6061 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 6121 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 6181 ccgcctctcc ccgcgcgttg gccgattcat taatgcagga tccgggatcg aagaaatgat 6241 ggtaaatgaa ataggaaatc aaggagcatg aaggcaaaag acaaatataa gggtcgaacg 6301 aaaaataaag tgaaaagtgt tgatatgatg tatttggctt tgcggcgccg aaaaaacgag 6361 tttacgcaat tgcacaatca tgctgactct gtggcggacc cgcgctcttg ccggcccggc 6421 gataacgctg ggcgtgaggc tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt 6481 ttaccctgcg ctaaggggcg agattggaga agcaataaga atgccggttg gggttgcgat 6541 gatgacgacc acgacaactg gtgtcattat ttaagttgcc gaaagaacct gagtgcattt 6601 gcaacatgag tatactagaa gaatgagcca agacttgcga gacgcgagtt tgccggtggt 6661 gcgaacaata gagcgaccat gaccttgaag gtgagacgcg cataaccgct agagtacttt 6721 gaagaggaaa cagcaatagg gttgctacca gtataaatag acaggtacat acaacactgg 6781 aaatggttgt ctgtttgagt acgctttcaa ttcatttggg tgtgcacttt attatgttac 6841 aatatggaag ggaactttac acttctccta tgcacatata ttaattaaag tccaatgcta 6901 gtagagaagg ggggtaacac ccctccgcgc tcttttccga tttttttcta aaccgtggaa 6961 tatttcggat atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca 7021 accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg 7081 gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac 7141 tacaccaatt acactgcctc attgatg //pOBD2 "Times New RomanMyCjGal4_DBD"Times New RomanMyCFXGal4_N_term_primer"Times New RomanMy yADH2_term"Times New RomanMyCɆpJG4_5_rev_pri"Times New RomanMyCL yADH1_terminator"Times New RomanMyCpHybLex_rev_pri"Times New Roman)MyCɆj CEN4"Times New RomanMy0  Orf frame 1"Times New Roman(MyC0 TRP1"Times New RomanMyC AmpR_promoter"Times New RomanMyCɆ g Orf frame 1"Times New Roman(My g Ampicillin"Times New RomanMyCm pBR322_origin"Times New Roman MyXhoI"Times New Roman 2mbM>M MyHpaI"Times New Roman 7"s+b.O@KO MyBEcoRI(926), PvuII(934), NcoI(942), SmaI(955), SalI(964), PstI(974)"Times New RomanuBN[u[u MyMscI"Times New RomanXSbpp MySpeI"Times New RomanD w&&' . MyNheI"Times New Roman'  - MyPmlI"Times New Roman TME'c MyAatII"Times New Roman >6,, Mytest"Times New RomanMA"x"f