Sequence of pOBD2

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pOBD2

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pOBD2 : 7167 BP; 2077 A; 1534 C; 1492 G; 2064 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt 60

1 caccatgtat tgcttgatta tgacatcggg atctgaacta tcggtagtag tatagcttca 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt 120

61 aagtgatggg aaaaaggtaa acggtagata acttcattat tatccgcgta cgttgaagaa 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa 180

121 aagaaaaaaa aagaaaagag agagggggca acaacagagt ggtataggcg ttactgtttt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt 240

181 tttactacct tctgtgattt ccttttttaa ttgctgtttc tgtcgtggtt gtctacagca 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattga 300

241 acaaggtctc gactactccc catagagctt cgtgtgcttt gaaaaaggaa ggaagtaact 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 360

301 ggacgttaat aattagaaaa caaaggagca gtaacaagag caagggaaag aaggaacaaa 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca 420

361 gaaaaagacg tgttataaag ttcgatatgg ttcgtatgtt agttgaggtt cgaacttcgt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact 480

421 tcggaggact ttctacttcg atgacagaag atagcttgtt cgtacgctat aaacggctga 480

.......... .......... .......... Gal4_DBD .......... ..........

.......... .......... .......... .......... .......... ..........

481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg 540

481 atttttcgag ttcacgaggt ttctttttgg cttcacgcgg ttcacagact tcttgttgac 540

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga 600

541 cctcacagcg atgagagggt tttggttttc cagaggcgac tgatcccgtg tagactgtct 600

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... XhoI(651)

601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga 660

601 tcaccttagt tccgatcttt ctgaccttgt cgataaagat gactaaaaag gagctcttct 660

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... HpaI(711)

661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt 720

661 ggaactgtac taaaactttt acctaagaaa tgtcctatat tttcgtaaca attgtcctaa 720

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac 780

721 taaacatgtt ctattacact tatttctacg gcagtgtcta tctaaccgaa gtcacctctg 780

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag 840

781 actatacgga gattgtaact ctgtcgtatc ttattcacgc tgtagtagta gccttctctc 840

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tagtaacaaa ggtcaaagac agttgactgt atcgaactat ctattcgatg atgaagatac 900

841 atcattgttt ccagtttctg tcaactgaca tagcttgata gataagctac tacttctatg 900

Gal4_N_term_primer Gal4_DBD .. .......... .......... ..........

.......... .......... EcoRI(926) PvuII(934) NcoI(942) ..........

901 cccaccaaac ccaaaaaaag agatcgaatt ccagctgacc accatggcaa ttcccgggga 960

901 gggtggtttg ggtttttttc tctagcttaa ggtcgactgg tggtaccgtt aagggcccct 960

.......... .......... .......... .......... .......... ..........

SalI(964) PstI(974) .......... .......... .......... ..........

961 tccgtcgacc tgcagagatc tatgaatcgt agatactgaa aaaccccgca agttcacttc 1020

961 aggcagctgg acgtctctag atacttagca tctatgactt tttggggcgt tcaagtgaag 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aactgtgcat cgtgcaccat ctcaatttct ttcatttata catcgttttg ccttctttta 1080

1021 ttgacacgta gcacgtggta gagttaaaga aagtaaatat gtagcaaaac ggaagaaaat 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... MscI(1112) .......... ..........

1081 tgtaactata ctcctctaag tttcaatctt ggccatgtaa cctctgatct atagaatttt 1140

1081 acattgatat gaggagattc aaagttagaa ccggtacatt ggagactaga tatcttaaaa 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttaaatgact agaattaatg cccatctttt ttttggacct aaattcttca tgaaaatata 1200

1141 aatttactga tcttaattac gggtagaaaa aaaacctgga tttaagaagt acttttatat 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttacgagggc ttattcagaa gctttggact tcttcgccag aggtttggtc aagtctccaa 1260

1201 aatgctcccg aataagtctt cgaaacctga agaagcggtc tccaaaccag ttcagaggtt 1260

.......... .......... ........ yADH2_term pJG4_5_rev_pri ........

.......... .......... .......... .......... .......... ..........

1261 tcaaggttgt cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa 1320

1261 agttccaaca gccgaacaga tggaacggtc tttaaatgct tttctacctt ttcccagttt 1320

.......... .......... yADH2_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcgttggtag atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt 1380

1321 agcaaccatc tatgcaacaa ctgtgaagat ttattcgctt aaagaatact aaatactaaa 1380

.......... .......... yADH2_term ....... yADH1_terminator .......

.......... .......... .......... .......... .......... ..........

1381 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg 1440

1381 aataataatt tattcaatat tttttttatt cacatatgtt taaaatttca ctgagaatcc 1440

......... yADH2_term yADH1_terminator pHybLex_rev_pri .......

.......... .......... .......... .......... .......... ..........

1441 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc 1500

1441 aaaattttgc ttttaagaat aagaactcat tgagaaagga catccagtcc aacgaaagag 1500

.......... . yADH2_term yADH1_terminator . .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgccg agcaaatgcc 1560

1501 tccatatcgt actccagcga gaataactgg tgtggagatg gccgtacggc tcgtttacgg 1560

.......... . yADH2_term yADH1_terminator . .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgcaaatcgc tccccatttc acccaattgt agatatgcta actccagcaa tgagttgatg 1620

1561 acgtttagcg aggggtaaag tgggttaaca tctatacgat tgaggtcgtt actcaactac 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aatctcggtg tgtattttat gtcctcagag gacaacacct gttgtaatcg ttcttccaca 1680

1621 ttagagccac acataaaata caggagtctc ctgttgtgga caacattagc aagaaggtgt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cggatcctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 1740

1681 gcctaggacc gcattatcgc ttctccgggc gtggctagcg ggaagggttg tcaacgcgtc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc 1800

1741 ggacttaccg cttaccgcgg actacgccat aaaagaggaa tgcgtagaca cgccataaag 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... SpeI(1860)

1801 acaccgcata tatcgctggg ccattctcat gaagaatatc ttgaatttat tgtcatatta 1860

1801 tgtggcgtat atagcgaccc ggtaagagta cttcttatag aacttaaata acagtataat 1860

.......... .......... .......... CEN4 .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctagttggtg tggaagtcca tatatcggtg atcaatatag tggttgacat gctggctagt 1920

1861 gatcaaccac accttcaggt atatagccac tagttatatc accaactgta cgaccgatca 1920

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca aatatcaaac 1980

1921 gttgtaactc ggaaaactag tacgtttata taatgccata aaatgttagt ttatagtttg 1980

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa gaaaaaaatt 2040

1981 aattgataac tgaaatattg aataaatcca ccattgtaag aatatttttt ctttttttaa 2040

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg tctcaccata 2100

2041 tgacgttttg tcatgatcga aaattgaaca taggatccaa tagatacgac agagtggtat 2100

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata catataatac 2160

2101 ctcttataat ggataaagtc ttacatacag gtactaagcg gcccatttat gtatattatg 2160

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa attggctagt 2220

2161 tgtttagacc gaattatttc agatattata tagagtattt cttcacgatt taaccgatca 2220

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg taaaagttca 2280

2221 cgatatataa aaattctttt aaagaaaact gattcaggta tagctgaaac attttcaagt 2280

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ctttagcata catatattac acgagccaga aattgtaact tttgcctaaa atcacaaatt 2340

2281 gaaatcgtat gtatataatg tgctcggtct ttaacattga aaacggattt tagtgtttaa 2340

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcaaaattta attgcttgca aaaggtcaca tgcttataat caactttttt aaaaatttaa 2400

2341 cgttttaaat taacgaacgt tttccagtgt acgaatatta gttgaaaaaa tttttaaatt 2400

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aatacttttt tattttttat ttttaaacat aaatgaaata atttatttat tgtttatgat 2460

2401 ttatgaaaaa ataaaaaata aaaatttgta tttactttat taaataaata acaaatacta 2460

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 taccgaaaca taaaacctgc tcaagaaaaa gaaactgttt tgtccttgga aaaaaagcac 2520

2461 atggctttgt attttggacg agttcttttt ctttgacaaa acaggaacct ttttttcgtg 2520

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tacctaggag cggccaaaat gccgaggctt tcatagctta aactctttac agaaaatagg 2580

2521 atggatcctc gccggtttta cggctccgaa agtatcgaat ttgagaaatg tcttttatcc 2580

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cattatagat cagttcgagt tttcttattc ttccttccgg ttttatcgtc acagttttac 2640

2581 gtaatatcta gtcaagctca aaagaataag aaggaaggcc aaaatagcag tgtcaaaatg 2640

.......... .......... CEN4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agtaaataag tatcacctct tagagttcga tgataagctg tcaaacatga gaattaattc 2700

2641 tcatttattc atagtggaga atctcaagct actattcgac agtttgtact cttaattaag 2700

.......... CEN4 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cacatgttaa aatagtgaag gagcatgttc ggcacacagt ggaccgaacg tggggtaagt 2760

2701 gtgtacaatt ttatcacttc ctcgtacaag ccgtgtgtca cctggcttgc accccattca 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcactagggt ccggttaaac ggatctcgca ttgatgaggc aacgctaatt atcaacatat 2820

2761 cgtgatccca ggccaatttg cctagagcgt aactactccg ttgcgattaa tagttgtata 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agattgttat ctatctgcat gaacacgaaa tctttacttg acgacttgag gctgatggtg 2880

2821 tctaacaata gatagacgta cttgtgcttt agaaatgaac tgctgaactc cgactaccac 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tttatgcaaa gaaaccactg tgtttaatat gtgtcactgt ttgatattac tgtcagcgta 2940

2881 aaatacgttt ctttggtgac acaaattata cacagtgaca aactataatg acagtcgcat 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gaagataata gtaaaagcgg ttaataagtg tatttgagat aagtgtgata aagtttttac 3000

2941 cttctattat cattttcgcc aattattcac ataaactcta ttcacactat ttcaaaaatg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 agcgaaaaga cgataaatac aagaaaatga ttacgaggat acggagagag gtatgtacat 3060

3001 tcgcttttct gctatttatg ttcttttact aatgctccta tgcctctctc catacatgta 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NheI(3111)

3061 gtgtatttat atactaagct gccggcggtt gtttgcaaga ccgagaaaag gctagcaaga 3120

3061 cacataaata tatgattcga cggccgccaa caaacgttct ggctcttttc cgatcgttct 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt gattccattg 3180

3121 tagcccagta acatcgcata cgcggacact tgtaagagaa gttgttcaaa ctaaggtaac 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa tcaatcaaaa 3240

3181 gccactttac cattttcagt tgggggacgc tacatataaa aggacatgtt agttagtttt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta tgtttagatc 3300

3241 tcggtttact aaatcgtaat agaaatgtag aacaataaaa tgtctaaaat acaaatctag 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttttatgctt gcttttcaaa aggcttgcag gcaagtgcac aaacaatact taaataaata 3360

3301 aaaatacgaa cgaaaagttt tccgaacgtc cgttcacgtg tttgttatga atttatttat 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt tgttagagtc 3420

3361 gatgagtcat tattggataa agaatcgtaa aaactgcttt aaacgataaa acaatctcag 3420

.......... .......... ....... Orf frame 1 TRP1 ....... ..........

.......... .......... .......... .......... .......... ..........

3421 ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc catttaatct 3480

3421 aaaatgtggt aaacagaggt gtggaggcga atgtagttgt ggttattgcg gtaaattaga 3480

.......... ....... Orf frame 1 TRP1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat gtaagctctc 3540

3481 ttcgcgtagt ggttgtaaaa gaccgcagtc aggtggtcga ttgtatttta cattcgagag 3540

.......... ....... Orf frame 1 TRP1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa gttcacctgt 3600

3541 ccccgagaga acggaaggtt gggtcagtct ttagctcaag gttaggtttt caagtggaca 3600

.......... ....... Orf frame 1 TRP1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg aagctgcact 3660

3601 gggtggacga agacttagtt tgttccctta tttgcttact ccaaagacac ttcgacgtga 3660

.......... ....... Orf frame 1 TRP1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca aaccgaggaa 3720

3661 ctcatcatac aacgtcagaa aacctttatg ctcagaaaat tattgaccgt ttggctcctt 3720

.......... ....... Orf frame 1 TRP1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatatcaa tgccgtaatc 3780

3721 gagaaccata agaacggtgc tgagtagagg tacgtcaacc tgctatagtt acggcattag 3780

.......... ....... Orf frame 1 TRP1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 attgaccaga gccaaaacat cctccttagg ttgattacga aacacgccaa ccaagtattt 3840

3781 taactggtct cggttttgta ggaggaatcc aactaatgct ttgtgcggtt ggttcataaa 3840

.......... ....... Orf frame 1 TRP1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cggagtgcct gaactatttt tatatgcttt tacaagactt gaaattttcc ttgcaataac 3900

3841 gcctcacgga cttgataaaa atatacgaaa atgttctgaa ctttaaaagg aacgttattg 3900

.......... ....... Orf frame 1 TRP1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cgggtcaatt gttctctttc tattgggcac acatataata cccagcaagt cagcatcgga 3960

3901 gcccagttaa caagagaaag ataacccgtg tgtatattat gggtcgttca gtcgtagcct 3960

.......... ....... Orf frame 1 TRP1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atctagtgca cattctgcgg cctctgtgct ctgcaagccg caaactttca ccaatggacc 4020

3961 tagatcacgt gtaagacgcc ggagacacga gacgttcggc gtttgaaagt ggttacctgg 4020

.......... ....... Orf frame 1 TRP1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 agaactacct gtgaaattaa taacagacat actccaagct gcctttgtgt gcttaatcac 4080

4021 tcttgatgga cactttaatt attgtctgta tgaggttcga cggaaacaca cgaattagtg 4080

....... Orf frame 1 TRP1 ....... .......... .......... ..........

PmlI(4090) .......... .......... .......... .......... ..........

4081 gtatactcac gtgctcaata gtcaccaatg ccctccctct tggccctctc cttttctttt 4140

4081 catatgagtg cacgagttat cagtggttac gggagggaga accgggagag gaaaagaaaa 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttcgaccgaa ttaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt 4200

4141 aagctggctt aattaagaac ttctgctttc ccggagcact atgcggataa aaatatccaa 4200

.......... .......... .......... .......... .......... ..........

.......... .......... ......... AatII(4231) ......... ..........

4201 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc 4260

4201 ttacagtact attattacca aagaatctgc agtccaccgt gaaaagcccc tttacacgcg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 4320

4261 ccttggggat aaacaaataa aaagatttat gtaagtttat acataggcga gtactctgtt 4320

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

4321 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 4380

4321 attgggacta tttacgaagt tattataact ttttccttct catactcata agttgtaaag 4380

.......... .......... .......... .......... .... Orf frame 1 Ampicillin

.......... .......... .......... .......... .......... ..........

4381 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 4440

4381 gcacagcggg aataagggaa aaaacgccgt aaaacggaag gacaaaaacg agtgggtctt 4440

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 4500

4441 tgcgaccact ttcattttct acgacttcta gtcaacccac gtgctcaccc aatgtagctt 4500

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 4560

4501 gacctagagt tgtcgccatt ctaggaactc tcaaaagcgg ggcttcttgc aaaaggttac 4560

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 4620

4561 tactcgtgaa aatttcaaga cgatacaccg cgccataata gggcataact gcggcccgtt 4620

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 4680

4621 ctcgttgagc cagcggcgta tgtgataaga gtcttactga accaactcat gagtggtcag 4680

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 4740

4681 tgtcttttcg tagaatgcct accgtactgt cattctctta atacgtcacg acggtattgg 4740

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 4800

4741 tactcactat tgtgacgccg gttgaatgaa gactgttgct agcctcctgg cttcctcgat 4800

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 4860

4801 tggcgaaaaa acgtgttgta ccccctagta cattgagcgg aactagcaac ccttggcctc 4860

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 4920

4861 gacttacttc ggtatggttt gctgctcgca ctgtggtgct acggacatcg ttaccgttgt 4920

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 4980

4921 tgcaacgcgt ttgataattg accgcttgat gaatgagatc gaagggccgt tgttaattat 4980

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 5040

4981 ctgacctacc tccgcctatt tcaacgtcct ggtgaagacg cgagccggga aggccgaccg 5040

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 5100

5041 accaaataac gactatttag acctcggcca ctcgcaccca gagcgccata gtaacgtcgt 5100

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 5160

5101 gaccccggtc taccattcgg gagggcatag catcaataga tgtgctgccc ctcagtccgt 5160

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 5220

5161 tgatacctac ttgctttatc tgtctagcga ctctatccac ggagtgacta attcgtaacc 5220

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 5280

5221 attgacagtc tggttcaaat gagtatatat gaaatctaac taaattttga agtaaaaatt 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 5340

5281 aaattttcct agatccactt ctaggaaaaa ctattagagt actggtttta gggaattgca 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 5400

5341 ctcaaaagca aggtgactcg cagtctgggg catcttttct agtttcctag aagaactcta 5400

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5401 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 5460

5401 ggaaaaaaag acgcgcatta gacgacgaac gtttgttttt ttggtggcga tggtcgccac 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 5520

5461 caaacaaacg gcctagttct cgatggttga gaaaaaggct tccattgacc gaagtcgtct 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 5580

5521 cgcgtctatg gtttatgaca ggaagatcac atcggcatca atccggtggt gaagttcttg 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5640

5581 agacatcgtg gcggatgtat ggagcgagac gattaggaca atggtcaccg acgacggtca 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 5700

5641 ccgctattca gcacagaatg gcccaacctg agttctgcta tcaatggcct attccgcgtc 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 5760

5701 gccagcccga cttgcccccc aagcacgtgt gtcgggtcga acctcgcttg ctggatgtgg 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 5820

5761 cttgactcta tggatgtcgc actcgatact ctttcgcggt gcgaagggct tccctctttc 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 5880

5821 cgcctgtcca taggccattc gccgtcccag ccttgtcctc tcgcgtgctc cctcgaaggt 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 5940

5881 ccccctttgc ggaccataga aatatcagga cagcccaaag cggtggagac tgaactcgca 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 6000

5941 gctaaaaaca ctacgagcag tccccccgcc tcggatacct ttttgcggtc gttgcgccgg 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 6060

6001 aaaaatgcca aggaccggaa aacgaccgga aaacgagtgt acaagaaagg acgcaatagg 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 6120

6061 ggactaagac acctattggc ataatggcgg aaactcactc gactatggcg agcggcgtcg 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 6180

6121 gcttgctggc tcgcgtcgct cagtcactcg ctccttcgcc ttctcgcggg ttatgcgttt 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ccgcctctcc ccgcgcgttg gccgattcat taatgcagga tccgggatcg aagaaatgat 6240

6181 ggcggagagg ggcgcgcaac cggctaagta attacgtcct aggccctagc ttctttacta 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ggtaaatgaa ataggaaatc aaggagcatg aaggcaaaag acaaatataa gggtcgaacg 6300

6241 ccatttactt tatcctttag ttcctcgtac ttccgttttc tgtttatatt cccagcttgc 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 aaaaataaag tgaaaagtgt tgatatgatg tatttggctt tgcggcgccg aaaaaacgag 6360

6301 tttttatttc acttttcaca actatactac ataaaccgaa acgccgcggc ttttttgctc 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 tttacgcaat tgcacaatca tgctgactct gtggcggacc cgcgctcttg ccggcccggc 6420

6361 aaatgcgtta acgtgttagt acgactgaga caccgcctgg gcgcgagaac ggccgggccg 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gataacgctg ggcgtgaggc tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt 6480

6421 ctattgcgac ccgcactccg acacgggccg cctcaaaaaa cgcggacgta aaaggttcca 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ttaccctgcg ctaaggggcg agattggaga agcaataaga atgccggttg gggttgcgat 6540

6481 aatgggacgc gattccccgc tctaacctct tcgttattct tacggccaac cccaacgcta 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gatgacgacc acgacaactg gtgtcattat ttaagttgcc gaaagaacct gagtgcattt 6600

6541 ctactgctgg tgctgttgac cacagtaata aattcaacgg ctttcttgga ctcacgtaaa 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gcaacatgag tatactagaa gaatgagcca agacttgcga gacgcgagtt tgccggtggt 6660

6601 cgttgtactc atatgatctt cttactcggt tctgaacgct ctgcgctcaa acggccacca 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gcgaacaata gagcgaccat gaccttgaag gtgagacgcg cataaccgct agagtacttt 6720

6661 cgcttgttat ctcgctggta ctggaacttc cactctgcgc gtattggcga tctcatgaaa 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gaagaggaaa cagcaatagg gttgctacca gtataaatag acaggtacat acaacactgg 6780

6721 cttctccttt gtcgttatcc caacgatggt catatttatc tgtccatgta tgttgtgacc 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 aaatggttgt ctgtttgagt acgctttcaa ttcatttggg tgtgcacttt attatgttac 6840

6781 tttaccaaca gacaaactca tgcgaaagtt aagtaaaccc acacgtgaaa taatacaatg 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 aatatggaag ggaactttac acttctccta tgcacatata ttaattaaag tccaatgcta 6900

6841 ttataccttc ccttgaaatg tgaagaggat acgtgtatat aattaatttc aggttacgat 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gtagagaagg ggggtaacac ccctccgcgc tcttttccga tttttttcta aaccgtggaa 6960

6901 catctcttcc ccccattgtg gggaggcgcg agaaaaggct aaaaaaagat ttggcacctt 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tatttcggat atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca 7020

6961 ataaagccta taggaaaaca acaaaggccc acatgttata cctgaaggag aaaagaccgt 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg 7080

7021 tggtttgggt atgtagccct aaggatatta tggaagcaac cagagggatt gtacatccac 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac 7140

7081 cgcctcccct ctatatgtta tcttgtctat ggtctgttct gtattacccg atttgttctg 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 tacaccaatt acactgcctc attgatg... .......... .......... .......... 7167

7141 atgtggttaa tgtgacggag taactac... .......... .......... .......... 7167

.......... .......... .......... .......... .......... ..........