Sequence of pPD90

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Sequence of pPD90_23

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Sequence of pPD90_23 : 6603 BP; 1604 A; 1655 C; 1728 G; 1616 T; 0 other;

......... HindIII(18) ..... PstI(34) XbaI(42) BamHI(48) SmaI(55)

1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggat 60

1 tactggtact aatgcggttc gaacgtacgg acgtccagct gagatctcct aggggcccta 60

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag 120

61 accggtttcc tgggtttcca tacaaagctt actatgattg tattgtatct tgtaaaagtc 120

.......... ......... Orf frame 1 SynIVS_A ......... ..........

.......... .......... .......... .......... .......... ..........

121 gaggaccctt ggagggtacc gagctcagaa aaaatgactg ctccaaagaa ga agcgtaag 180

121 ctcctgggaa cctcccatgg ctcgagtctt ttttactgac gaggtttctt cttcgcattc 180

.......... ......... Orf frame 1 ......... SV40_NLS ..........

AgeI(183) .......... .......... .......... .......... ..........

181 gtaccggtgg gtgaagacca gaaacagcac ctcgaactga gccgcgatat tgcccagcgt 240

181 catggccacc cacttctggt ctttgtcgtg gagcttgact cggcgctata acgggtcgca 240

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ttcaacgcgc tgtatggcga gatcgatccc gtcgttttac aacgtcgtga ct gggaaaac 300

241 aagttgcgcg acataccgct ctagctaggg cagcaaaatg ttgcagcact gacccttttg 300

.......... ......... Orf frame 1 ......... lacZ ..........

.......... .......... .......... .......... .......... ..........

301 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 360

301 ggaccgcaat gggttgaatt agcggaacgt cgtgtagggg gaaagcggtc gaccgcatta 360

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

361 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 420

361 tcgcttctcc gggcgtggct agcgggaagg gttgtcaacg cgtcggactt accgcttacc 420

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt 480

421 gcgaaacgga ccaaaggccg tggtcttcgc cacggccttt cgaccgacct cacgctagaa 480

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc 540

481 ggactccggc tatgacagca gcaggggagt ttgaccgtct acgtgccaat gctacgcggg 540

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat 600

541 tagatgtggt tgcactggat agggtaatgc cagttaggcg gcaaacaagg gtgcctctta 600

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag 660

601 ggctgcccaa caatgagcga gtgtaaatta caactacttt cgaccgatgt ccttccggtc 660

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg 720

661 tgcgcttaat aaaaactacc gcaattgagc cgcaaagtag acaccacgtt gcccgcgacc 720

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc 780

721 cagccaatgc cggtcctgtc agcaaacggc agacttaaac tggactcgcg taaaaatgcg 780

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa 840

781 cggcctcttt tggcggagcg ccactaccac gacgcgacct cactgccgtc aatagacctt 840

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg 900

841 ctagtcctat acaccgccta ctcgccgtaa aaggcactgc agagcaacga cgtatttggc 900

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct 960

901 tgatgtgttt agtcgctaaa ggtacaacgg tgagcgaaat tactactaaa gtcggcgcga 960

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt 1020

961 catgacctcc gacttcaagt ctacacgccg ctcaacgcac tgatggatgc ccattgtcaa 1020

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt 1080

1021 agaaataccg tcccactttg cgtccagcgg tcgccgtggc gcggaaagcc gccactttaa 1080

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg 1140

1081 tagctactcg caccaccaat acggctagcg cagtgtgatg cagacttgca gcttttgggc 1140

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc 1200

1141 tttgacacct cgcggcttta gggcttagag atagcacgcc accaacttga cgtgtggcgg 1200

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa 1260

1201 ctgccgtgcg actaacttcg tcttcggacg ctacagccaa aggcgctcca cgcctaactt 1260

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag 1320

1261 ttaccagacg acgacgactt gccgttcggc aacgactaag ctccgcaatt ggcagtgctc 1320

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... ......... EcoRV(1371)

1321 catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg 1380

1321 gtagtaggag acgtaccagt ccagtaccta ctcgtctgct accacgtcct ataggacgac 1380

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg 1440

1381 tacttcgtct tgttgaaatt gcggcacgcg acaagcgtaa taggcttggt aggcgacacc 1440

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac 1500

1441 atgtgcgaca cgctggcgat gccggacata caccacctac ttcggttata actttgggtg 1500

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa 1560

1501 ccgtaccacg gttacttagc agactggcta ctaggcgcga ccgatggccg ctactcgctt 1560

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... BclI(1604) ..........

1561 cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg 1620

1561 gcgcattgcg cttaccacgt cgcgctagca ttagtgggct cacactagta gaccagcgac 1620

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc 1680

1621 cccttactta gtccggtgcc gcgattagtg ctgcgcgaca tagcgaccta gtttagacag 1680

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt 1740

1681 ctaggaaggg cgggccacgt catacttccg ccgcctcggc tgtggtgccg gtggctataa 1740

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg 1800

1741 taaacgggct acatgcgcgc gcacctactt ctggtcggga agggccgaca cggctttacc 1800

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac 1860

1801 aggtagtttt ttaccgaaag cgatggacct ctctgcgcgg gcgactagga aacgcttatg 1860

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag 1920

1861 cgggtgcgct acccattgtc agaaccgcca aagcgattta tgaccgtccg caaagcagtc 1920

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat 1980

1921 ataggggcaa atgtcccgcc gaagcagacc ctgacccacc tagtcagcga ctaatttata 1980

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat 2040

1981 ctacttttgc cgttgggcac cagccgaatg ccgccactaa aaccgctatg cggcttgcta 2040

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg 2100

2041 gcggtcaaga catacttgcc agaccagaaa cggctggcgt gcggcgtagg tcgcgactgc 2100

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg 2160

2101 cttcgttttg tggtcgtcgt caaaaaggtc aaggcaaata ggcccgtttg gtagcttcac 2160

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg 2220

2161 tggtcgctta tggacaaggc agtatcgcta ttgctcgagg acgtgaccta ccaccgcgac 2220

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag 2280

2221 ctaccattcg gcgaccgttc gccacttcac ggagacctac agcgaggtgt tccatttgtc 2280

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta 2340

2281 aactaacttg acggacttga tggcgtcggc ctctcgcggc ccgttgagac cgagtgtcat 2340

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag 2400

2341 gcgcatcacg ttggcttgcg ctggcgtacc agtcttcggc ccgtgtagtc gcggaccgtc 2400

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg 2460

2401 gtcaccgcag accgcctttt ggagtcacac tgcgaggggc ggcgcagggt gcggtagggc 2460

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt 2520

2461 gtagactggt ggtcgcttta cctaaaaacg tagctcgacc cattattcgc aaccgttaaa 2520

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg 2580

2521 ttggcggtca gtccgaaaga aagtgtctac acctaaccgc tattttttgt tgacgactgc 2580

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg 2640

2581 ggcgacgcgc tagtcaagtg ggcacgtggc gacctattgc tgtaaccgca ttcacttcgc 2640

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc 2700

2641 tgggcgtaac tgggattgcg gacccagctt gcgaccttcc gccgcccggt aatggtccgg 2700

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct gattacgacc 2760

2701 cttcgtcgca acaacgtcac gtgccgtcta tgtgaacgac tacgccacga ctaatgctgg 2760

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt 2820

2761 cgagtgcgca ccgtcgtagt ccccttttgg aataaatagt cggccttttg gatggcctaa 2820

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat 2880

2821 ctaccatcac cagtttaccg ctaatggcaa ctacaacttc accgctcgct atgtggcgta 2880

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc 2940

2881 ggccgcgcct aaccggactt gacggtcgac cgcgtccatc gtctcgccca tttgaccgag 2940

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg 3000

2941 cctaatcccg gcgttctttt gatagggctg gcggaatgac ggcggacaaa actggcgacc 3000

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc 3060

3001 ctagacggta acagtctgta catatggggc atgcagaagg gctcgctttt gccagacgcg 3060

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac 3120

3061 acgccctgcg cgcttaactt aataccgggt gtggtcaccg cgccgctgaa ggtcaagttg 3120

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg 3180

3121 tagtcggcga tgtcagttgt cgttgactac ctttggtcgg tagcggtaga cgacgtgcgc 3180

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc 3240

3181 cttcttccgt gtaccgactt atagctgcca aaggtatacc cctaaccacc gctgctgagg 3240

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... ......... EcoRI(3262) ......... .......... ..........

3241 tggagcccgt cagtatcggc ggaattccaa ctgagcgccg gtcgctacca ttaccaactt 3300

3241 acctcgggca gtcatagccg ccttaaggtt gactcgcggc cagcgatggt aatggttgaa 3300

.......... ....... Orf frame 1 lacZ ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gtctggtgtc aaaaataata ggggccgctg tcatcagagt aagtttaaac tgagttctac 3360

3301 cagaccacag tttttattat ccccggcgac agtagtctca ttcaaatttg actcaagatg 3360

Orf frame 1 lacZ .... .......... .......... SynIVS.L ..........

.......... .......... .......... .......... .......... ..........

3361 taactaacga gtaatattta aattttcagc atctcgcgcc cgtgcctctg acttctaagt 3420

3361 attgattgct cattataaat ttaaaagtcg tagagcgcgg gcacggagac tgaagattca 3420

.......... SynIVS.L .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt 3480

3421 ggttaatgag aagttgtagg gatgtacgag aaagagggac acgagggtgg gggataaaaa 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct 3540

3481 caataatagt tttttgaaga gaattaaaga aacaaaaaat cgaagaaaat tcagtggaga 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa 3600

3541 ttgttacttt aacacatcta agtttttatc ttaattaagc attatttttc agcttttttt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt 3660

3601 aacacgaggg aggggggtaa ttattattaa gatagggttt tagatgtgtt acaagacaca 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca 3720

3661 tgtgaagaat acaaaaaatg aagactattt aaaaaaactt tgtagtatct tttttggcgt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc 3780

3721 gtgttttatg gaatagtata caatgcaaag tcaaatactg gcgttaaaaa taaagaagcg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt 3840

3781 tgcagacccg gagagtactg cagtttagta cgagtagcac tttttcaaaa cctcataaaa 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg 3900

3841 accttaaaaa gttagttcac tttcaaatac tttaattaaa aggacgaaaa cgaaaaaccc 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt 3960

3901 caaaggggat aacaaacagt tctaaagctc ctgccgcaaa aagaacgatt ttagtgttca 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag 4020

3961 taactactcg tgctacgttc tttctagcct tcttccaaac ccaaactccg agtcaccttc 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg 4080

4021 cactcatctt caactattaa actttcacct catcacagat accccaaaaa cggaatttac 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ApaI(4140)

4081 acagaataca ttcccaatat accaaacata actgtttcct actagtcggc cgtacgggcc 4140

4081 tgtcttatgt aagggttata tggtttgtat tgacaaagga tgatcagccg gcatgcccgg 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 4200

4141 gaaagcagag cgcgcaaagc cactactgcc acttttggag actgtgtacg tcgagggcct 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 4260

4201 ctgccagtgt cgaacagaca ttcgcctacg gccctcgtct gttcgggcag tcccgcgcag 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 4320

4261 tcgcccacaa ccgcccacag ccccgaccga attgatacgc cgtagtctcg tctaacatga 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 4380

4321 ctctcacgtg gtatacgcca cactttatgg cgtgtctacg cattcctctt ttatggcgta 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 caggcggcct taagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 4440

4381 gtccgccgga attcccggag cactatgcgg ataaaaatat ccaattacag tactattatt 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 4500

4441 accaaagaat ctgcagtcca ccgtgaaaag cccctttaca cgcgccttgg ggataaacaa 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 4560

4501 ataaaaagat ttatgtaagt ttatacatag gcgagtactc tgttattggg actatttacg 4560

.......... .......... ......... Amp_promoter ......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 4620

4561 aagttattat aactttttcc ttctcatact cataagttgt aaaggcacag cgggaataag 4620

.......... .......... .......... .... Orf frame 3 Ampicillin ....

.......... .......... .......... .......... .......... ..........

4621 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 4680

4621 ggaaaaaacg ccgtaaaacg gaaggacaaa aacgagtggg tctttgcgac cactttcatt 4680

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 4740

4681 ttctacgact tctagtcaac ccacgtgctc acccaatgta gcttgaccta gagttgtcgc 4740

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 4800

4741 cattctagga actctcaaaa gcggggcttc ttgcaaaagg ttactactcg tgaaaatttc 4800

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 4860

4801 aagacgatac accgcgccat aatagggcat aactgcggcc cgttctcgtt gagccagcgg 4860

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 4920

4861 cgtatgtgat aagagtctta ctgaaccaac tcatgagtgg tcagtgtctt ttcgtagaat 4920

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 4980

4921 gcctaccgta ctgtcattct cttaatacgt cacgacggta ttggtactca ctattgtgac 4980

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 5040

4981 gccggttgaa tgaagactgt tgctagcctc ctggcttcct cgattggcga aaaaacgtgt 5040

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 5100

5041 tgtaccccct agtacattga gcggaactag caacccttgg cctcgactta cttcggtatg 5100

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 5160

5101 gtttgctgct cgcactgtgg tgctacggac atcgttaccg ttgttgcaac gcgtttgata 5160

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 5220

5161 attgaccgct tgatgaatga gatcgaaggg ccgttgttaa ttatctgacc tacctccgcc 5220

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 5280

5221 tatttcaacg tcctggtgaa gacgcgagcc gggaaggccg accgaccaaa taacgactat 5280

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 5340

5281 ttagacctcg gccactcgca cccagagcgc catagtaacg tcgtgacccc ggtctaccat 5340

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 5400

5341 tcgggagggc atagcatcaa tagatgtgct gcccctcagt ccgttgatac ctacttgctt 5400

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 5460

5401 tatctgtcta gcgactctat ccacggagtg actaattcgt aaccattgac agtctggttc 5460

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 5520

5461 aaatgagtat atatgaaatc taactaaatt ttgaagtaaa aattaaattt tcctagatcc 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 5580

5521 acttctagga aaaactatta gagtactggt tttagggaat tgcactcaaa agcaaggtga 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 5640

5581 ctcgcagtct ggggcatctt ttctagtttc ctagaagaac tctaggaaaa aaagacgcgc 5640

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5641 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 5700

5641 attagacgac gaacgtttgt ttttttggtg gcgatggtcg ccaccaaaca aacggcctag 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 5760

5701 ttctcgatgg ttgagaaaaa ggcttccatt gaccgaagtc gtctcgcgtc tatggtttat 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 5820

5761 gacaggaaga tcacatcggc atcaatccgg tggtgaagtt cttgagacat cgtggcggat 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 5880

5821 gtatggagcg agacgattag gacaatggtc accgacgacg gtcaccgcta ttcagcacag 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 5940

5881 aatggcccaa cctgagttct gctatcaatg gcctattccg cgtcgccagc ccgacttgcc 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 6000

5941 ccccaagcac gtgtgtcggg tcgaacctcg cttgctggat gtggcttgac tctatggatg 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 6060

6001 tcgcactcgt aactctttcg cggtgcgaag ggcttccctc tttccgcctg tccataggcc 6060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6061 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 6120

6061 attcgccgtc ccagccttgt cctctcgcgt gctccctcga aggtccccct ttgcggacca 6120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6121 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 6180

6121 tagaaatatc aggacagccc aaagcggtgg agactgaact cgcagctaaa aacactacga 6180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6181 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 6240

6181 gcagtccccc cgcctcggat acctttttgc ggtcgttgcg ccggaaaaat gccaaggacc 6240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 6300

6241 ggaaaacgac cggaaaacga gtgtacaaga aaggacgcaa taggggacta agacacctat 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 6360

6301 tggcataatg gcggaaactc actcgactat ggcgagcggc gtcggcttgc tggctcgcgt 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 6420

6361 cgctcagtca ctcgctcctt cgccttctcg cgggttatgc gtttggcgga gaggggcgcg 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 6480

6421 caaccggcta agtaattacg tcgaccgtgc tgtccaaagg gctgaccttt cgcccgtcac 6480

.......... .......... lacI_C .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 6540

6481 tcgcgttgcg ttaattacac tcaatcgagt gagtaatccg tggggtccga aatgtgaaat 6540

.......... .......... .......... .......... .......... lac

.......... .......... .......... .......... .......... ..........

6541 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 6600

6541 acgaaggccg agcatacaac acaccttaac actcgcctat tgttaaagtg tgtcctttgt 6600

lac .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gct....... .......... .......... .......... .......... .......... 6603

6601 cga....... .......... .......... .......... .......... .......... 6603

.......... .......... .......... .......... .......... ..........