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pPGK-Cre-bpA
Gene/insert name: Phosphoglycerate kinase 1 promoter
Alternative names: PGK
Insert size (bp): 1944
Species of gene(s): bacteriophage P1
Vector backbone: XhopKS (Search Vector Database)
Backbone manufacturer: Stratagene
Type of vector: Mammalian expression,Cre/Lox
Backbone size (bp): 4870
Cloning site 5': Sall
Site destroyed during cloning: No
Cloning site 3': Not I
Site destroyed during cloning: No
5' Sequencing primer: n/a (List of Sequencing Primers)
3' Sequencing primer: BGH-rev
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Klaus Rajewsky
Comments: Full Gene Name: PGK-Cre-bpA: Phosphoglycerate kinase I promoter (PGK), the bacteriophage P1 recombinase Cre with a SV40 large T antigen nuclear localization signal (NLS-Cre), bovine growth hormone polyadenylation site (bpA). Use to express the Cre recombinase in mammalian cells. The vector backbone is a variant of pBluescriptKS- (Stratagene), in which the Xbal site was converted to an Xhol site (provided by Paul Hasty) (Soriano P., Cell 1997). The plasmid was generated by Kurt Fellenberg. He cloned a NLS-Cre sequence, as published by Hua Gu (Gu H, Zou YR, Rajewsky K. 1993. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell. 73:1155-1164) and cloned it into the pPGK-neo-bpA (Soriano P, Montgomery C, Geske R, Bradley A. 1991 Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice. Cell. Feb 22;64(4):693-702.), replacing the neomycin resistance gene (neo) with th
e NLS-Cre.