Sequence of pPGK-Cre-bpA

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Sequence of pPGK-Cre-bpA

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Sequence of pPGK-Cre-bpA : 4870 BP; 1146 A; 1245 C; 1287 G; 1192 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atgcaatcct actatgcaat gtatccaggt ggcacttttc ggggaaatgt gcgcggaacc 60

1 tacgttagga tgatacgtta cataggtcca ccgtgaaaag cccctttaca cgcgccttgg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 120

61 ggataaacaa ataaaaagat ttatgtaagt ttatacatag gcgagtactc tgttattggg 120

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

121 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 180

121 actatttacg aagttattat aactttttcc ttctcatact cataagttgt aaaggcacag 180

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

181 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 240

181 cgggaataag ggaaaaaacg ccgtaaaacg gaaggacaaa aacgagtggg tctttgcgac 240

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 300

241 cactttcatt ttctacgact tctagtcaac ccacgtgctc acccaatgta gcttgaccta 300

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 360

301 gagttgtcgc cattctagga actctcaaaa gcggggcttc ttgcaaaagg ttactactcg 360

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 420

361 tgaaaatttc aagacgatac accgcgccat aatagggcat aactgcggcc cgttctcgtt 420

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 480

421 gagccagcgg cgtatgtgat aagagtctta ctgaaccaac tcatgagtgg tcagtgtctt 480

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 540

481 ttcgtagaat gcctaccgta ctgtcattct cttaatacgt cacgacggta ttggtactca 540

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 600

541 ctattgtgac gccggttgaa tgaagactgt tgctagcctc ctggcttcct cgattggcga 600

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 660

601 aaaaacgtgt tgtaccccct agtacattga gcggaactag caacccttgg cctcgactta 660

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 720

661 cttcggtatg gtttgctgct cgcactgtgg tgctacggac atcgttaccg ttgttgcaac 720

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 780

721 gcgtttgata attgaccgct tgatgaatga gatcgaaggg ccgttgttaa ttatctgacc 780

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 840

781 tacctccgcc tatttcaacg tcctggtgaa gacgcgagcc gggaaggccg accgaccaaa 840

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 900

841 taacgactat ttagacctcg gccactcgca cccagagcgc catagtaacg tcgtgacccc 900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 960

901 ggtctaccat tcgggagggc atagcatcaa tagatgtgct gcccctcagt ccgttgatac 960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 1020

961 ctacttgctt tatctgtcta gcgactctat ccacggagtg actaattcgt aaccattgac 1020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 1080

1021 agtctggttc aaatgagtat atatgaaatc taactaaatt ttgaagtaaa aattaaattt 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 1140

1081 tcctagatcc acttctagga aaaactatta gagtactggt tttagggaat tgcactcaaa 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 1200

1141 agcaaggtga ctcgcagtct ggggcatctt ttctagtttc ctagaagaac tctaggaaaa 1200

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1201 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 1260

1201 aaagacgcgc attagacgac gaacgtttgt ttttttggtg gcgatggtcg ccaccaaaca 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 1320

1261 aacggcctag ttctcgatgg ttgagaaaaa ggcttccatt gaccgaagtc gtctcgcgtc 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 1380

1321 tatggtttat gacaggaaga tcacatcggc atcaatccgg tggtgaagtt cttgagacat 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 1440

1381 cgtggcggat gtatggagcg agacgattag gacaatggtc accgacgacg gtcaccgcta 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 1500

1441 ttcagcacag aatggcccaa cctgagttct gctatcaatg gcctattccg cgtcgccagc 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 1560

1501 ccgacttgcc ccccaagcac gtgtgtcggg tcgaacctcg cttgctggat gtggcttgac 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 1620

1561 tctatggatg tcgcactcga tactctttcg cggtgcgaag ggcttccctc tttccgcctg 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 1680

1621 tccataggcc attcgccgtc ccagccttgt cctctcgcgt gctccctcga aggtccccct 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 1740

1681 ttgcggacca tagaaatatc aggacagccc aaagcggtgg agactgaact cgcagctaaa 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 1800

1741 aacactacga gcagtccccc cgcctcggat acctttttgc ggtcgttgcg ccggaaaaat 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 1860

1801 gccaaggacc ggaaaacgac cggaaaacga gtgtacaaga aaggacgcaa taggggacta 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 1920

1861 agacacctat tggcataatg gcggaaactc actcgactat ggcgagcggc gtcggcttgc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 1980

1921 tggctcgcgt cgctcagtca ctcgctcctt cgccttctcg cgggttatgc gtttggcgga 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 2040

1981 gaggggcgcg caaccggcta agtaattacg tcgaccgtgc tgtccaaagg gctgaccttt 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 2100

2041 cgcccgtcac tcgcgttgcg ttaattacac tcaatcgagt gagtaatccg tggggtccga 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 2160

2101 aatgtgaaat acgaaggccg agcatacaac acaccttaac actcgcctat tgttaaagtg 2160

.......... lac .......... .......... ....... M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

2161 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa 2220

2161 tgtcctttgt cgatactggt actaatgcgg ttcgcgcgtt aattgggagt gatttccctt 2220

....... M13_reverse_pri ....... .......... T3_pro ..........

.... KpnI(2234) ApaI(2240) .... ....... HindIII(2266) EcoRI(2278)

2221 caaaagctgg gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa 2280

2221 gttttcgacc catggcccgg gggggagctc cagctgccat agctattcga actatagctt 2280

.......... .......... ...... pBluescriptKS_pri ...... ..........

.......... .......... .......... .......... .......... ..........

2281 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc 2340

2281 aagatggccc atcccctccg cgaaaagggt tccgtcagac ctcgtacgcg aaatcgtcgg 2340

.......... .......... .......... .......... .......... MSCV_r

.......... .......... .......... .......... .......... ..........

2341 ccgctggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg 2400

2341 ggcgaccgtg aaccgcgatg tgttcaccgg agaccggagc gtgtgtaagg tgtaggtggc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtagcgccaa ccggctccgt tctttggtgg ccccttcgcg ccacttctac tcctccccta 2460

2401 catcgcggtt ggccgaggca agaaaccacc ggggaagcgc ggtgaagatg aggaggggat 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtcaggaagt ttcccccagc aagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac 2520

2461 cagtccttca aagggggtcg ttcgagcgca gcacgtcctg cactgtttac cttcatcgtg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... StuI(2575)

2521 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt 2580

2521 cagagtgatc agagcacgtc tacctgtcgt ggcgactcgt taccttcgcc catccggaaa 2580

.......... .......... .......... .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

2581 ggggcagcgg ccaatagcag ctttgttcct tcgctttctg ggctcagagg ctgggaaggg 2640

2581 ccccgtcgcc ggttatcgtc gaaacaagga agcgaaagac ccgagtctcc gacccttccc 2640

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... NarI(2685) ..........

2641 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg gcgggcgccc gaaggtcctc 2700

2641 cacccaggcc cccgcccgag tccccgcccg agtccccgcc cgcccgcggg cttccaggag 2700

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccgaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 2760

2701 ggctccgggc cgtaagacgt gcgaagtttt cgcgtgcaga cggcgcgaca agaggagaag 2760

.......... ....... ORF 2 mPGK_F_pri ....... .......... ..........

.......... .......... PstI(2786) .......... .......... ..........

2761 ctcatctccg ggcctttcga cctgcagctc gaggtcgacc atgcccaaga agaagaggaa 2820

2761 gagtagaggc ccggaaagct ggacgtcgag ctccagctgg tacgggttct tcttctcctt 2820

.......... .......... ORF 2 .......... .......... CRE_NLS

.......... .......... .......... .......... .......... ..........

2821 ggtgtccaat ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag 2880

2821 ccacaggtta aatgactggc atgtggtttt aaacggacgt aatggccagc tacgttgctc 2880

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgatgaggtt cgcaagaacc tgatggacat gttcagggat cgccaggcgt tttctgagca 2940

2881 actactccaa gcgttcttgg actacctgta caagtcccta gcggtccgca aaagactcgt 2940

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa 3000

2941 atggaccttt tacgaagaca ggcaaacggc cagcacccgc cgtaccacgt tcaacttatt 3000

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... NruI(3036) .......... ..........

3001 ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat tatcttctat atcttcaggc 3060

3001 ggcctttacc aaagggcgtc ttggacttct acaagcgcta atagaagata tagaagtccg 3060

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gcgcggtctg gcagtaaaaa ctatccagca acatttgggc cagctaaaca tgcttcatcg 3120

3061 cgcgccagac cgtcattttt gataggtcgt tgtaaacccg gtcgatttgt acgaagtagc 3120

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcggtccggg ctgccacgac caagtgacag caatgctgtt tcactggtta tgcggcggat 3180

3121 agccaggccc gacggtgctg gttcactgtc gttacgacaa agtgaccaat acgccgccta 3180

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... ......... BstBI(3231)

3181 ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac 3240

3181 ggcttttctt ttgcaactac ggccacttgc acgttttgtc cgagatcgca agcttgcgtg 3240

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgatttcgac caggttcgtt cactcatgga aaatagcgat cgctgccagg atatacgtaa 3300

3241 actaaagctg gtccaagcaa gtgagtacct tttatcgcta gcgacggtcc tatatgcatt 3300

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tctggcattt ctggggattg cttataacac cctgttacgt atagccgaaa ttgccaggat 3360

3301 agaccgtaaa gacccctaac gaatattgtg ggacaatgca tatcggcttt aacggtccta 3360

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cagggttaaa gatatctcac gtactgacgg tgggagaatg ttaatccata ttggcagaac 3420

3361 gtcccaattt ctatagagtg catgactgcc accctcttac aattaggtat aaccgtcttg 3420

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gaaaacgctg gttagcaccg caggtgtaga gaaggcactt agcctggggg taactaaact 3480

3421 cttttgcgac caatcgtggc gtccacatct cttccgtgaa tcggaccccc attgatttga 3480

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggtcgagcga tggatttccg tctctggtgt agctgatgat ccgaataact acctgttttg 3540

3481 ccagctcgct acctaaaggc agagaccaca tcgactacta ggcttattga tggacaaaac 3540

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc agccagctat caactcgcgc 3600

3541 ggcccagtct tttttaccac aacggcgcgg tagacggtgg tcggtcgata gttgagcgcg 3600

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cctggaaggg atttttgaag caactcatcg attgatttac ggcgctaagg atgactctgg 3660

3601 ggaccttccc taaaaacttc gttgagtagc taactaaatg ccgcgattcc tactgagacc 3660

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tcagagatac ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc 3720

3661 agtctctatg gaccggacca gacctgtgtc acgggcacag cctcggcgcg ctctataccg 3720

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccgcgctgga gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat 3780

3721 ggcgcgacct caaagttatg gcctctagta cgttcgacca ccgacctggt tacatttata 3780

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgtcatgaac tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga 3840

3781 acagtacttg atataggcat tggacctatc actttgtccc cgttaccacg cggacgacct 3840

.......... ........ ORF 2 CRE_NLS ........ .......... ..........

.......... .......... .......... .......... XbaI(3888) BclI(3900)

3841 agatggcgat tagccattaa cgcgtaaatg attgcagatc cactagttct agagctcgct 3900

3841 tctaccgcta atcggtaatt gcgcatttac taacgtctag gtgatcaaga tctcgagcga 3900

ORF 2 CRE_NLS ...... .......... ...... pBluescriptSK_pri ......

.......... .......... .......... .......... .......... ..........

3901 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 3960

3901 ctagtcggag ctgacacgga agatcaacgg tcggtagaca acaaacgggg agggggcacg 3960

......... BGH_rev_pri ........ bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

3961 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 4020

3961 gaaggaactg ggaccttcca cggtgagggt gacaggaaag gattatttta ctcctttaac 4020

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 4080

4021 gtagcgtaac agactcatcc acagtaagat aagacccccc accccacccc gtcctgtcgt 4080

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 4140

4081 tccccctcct aacccttctg ttatcgtccg tacgacccct acgccacccg agataccgaa 4140

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctgaggaaag aaccagctgg ggctcgagat ccactagttc tagcctcgag gctagagcgg 4200

4141 gactcctttc ttggtcgacc ccgagctcta ggtgatcaag atcggagctc cgatctcgcc 4200

.......... .......... .......... .......... .......... ..........

SacII(4210) ........ .......... .......... .......... ..........

4201 ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacgc gcgctcactg 4260

4201 ggcggtggcg ccacctcgag gttaagcggg atatcactca gcataatgcg cgcgagtgac 4260

.......... .......... .......... T7 .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 4320

4261 cggcagcaaa atgttgcagc actgaccctt ttgggaccgc aatgggttga attagcggaa 4320

M13_forward20_pri M13_pUC_fwd_pri lacZ_a ...... ..........

.......... .......... .......... .......... .......... ..........

4321 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 4380

4321 cgtcgtgtag ggggaaagcg gtcgaccgca ttatcgcttc tccgggcgtg gctagcggga 4380

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc 4440

4381 agggttgtca acgcgtcgga cttaccgctt accctgcgcg ggacatcgcc gcgtaattcg 4440

.......... lacZ_a .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

4441 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 4500

4441 cgccgcccac accaccaatg cgcgtcgcac tggcgatgtg aacggtcgcg ggatcgcggg 4500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 4560

4501 cgaggaaagc gaaagaaggg aaggaaagag cggtgcaagc ggccgaaagg ggcagttcga 4560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 4620

4561 gatttagccc ccgagggaaa tcccaaggct aaatcacgaa atgccgtgga gctggggttt 4620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 4680

4621 tttgaactaa tcccactacc aagtgcatca cccggtagcg ggactatctg ccaaaaagcg 4680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 4740

4681 ggaaactgca acctcaggtg caagaaatta tcacctgaga acaaggtttg accttgttgt 4740

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 4800

4741 gagttgggat agagccagat aagaaaacta aatattccct aaaacggcta aagccggata 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 4860

4801 accaattttt tactcgacta aattgttttt aaattgcgct taaaattgtt ttataattgc 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cttacaattt .......... .......... .......... .......... .......... 4870

4861 gaatgttaaa .......... .......... .......... .......... .......... 4870

.......... .......... .......... .......... .......... ..........