Y^'LOCUS pPacPL 6387 bp DNA SYN 31-Aug-2007 DEFINITION pPacPL ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..6387 /organism="pPacPL" /mol_type="other DNA" misc_feature complement(4..20) /label="M13_forward20_primer" misc_feature complement(13..35) /label="M13_pUC_fwd_primer" misc_feature 13..156 /label="lacZ_a" misc_feature 348..370 /label="pGEX_3_primer" promoter 529..557 /label="AmpR_promoter" CDS 599..1459 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 599..1459 /label="Ampicillin" /gene="Ampicillin" rep_origin 1614..2233 /label="pBR322_origin" promoter 2542..2571 /label="lac_promoter" misc_feature 2585..2607 /label="M13_pUC_rev_primer" misc_feature 2606..2624 /label="M13_reverse_primer" CDS 3326..4054 /label="ORF frame 2" /translation="MMPDHRLISASFAERSACAVVVFWNSFNFRTVRFDFSFLAYSKS KVKPKKRDGNTKCGKTVVEGKGCGAAEGRVGRGVAGSVAGRDVTDVGATPLTMCACVY YSCLATRRLFFSFYLALSSAISYACSTHRMLPCPLCVILARNRQLFKQRLVNENAKIN KSTIWLLIAMCVQPTIATKATTQWLSLFHFLFASACEQDGTTGKRNYADKEQTKFWPK NIKAPDTNAFAITIAIFNIVYEAV*" misc_feature 5143..5162 /label="AC5_primer" ORIGIN 1 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 61 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 121 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct 181 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc 241 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg 301 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 361 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg 421 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1141 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2401 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 2461 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 2521 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 2581 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcnnnnn 2641 taaaaaaaaa tcatgaatgg catcaactct gaatcaaatc tttgcagatg cacctacttc 2701 tcatttccac tgtcacatca tttttccaga tctcgctgcc tgttatgtgg cccacaaacc 2761 aagacacgtt ttatggccat taaagctggc tgatcgtcgc caaacaccaa atacataatg 2821 aatatgtaca cattcgagaa agaagcgatc aaagaagcgt cttcgggcgg agtaggagaa 2881 tgcggaggag aaggagaacg agctgatcta gtatctctcc acaatccaat gccaactgac 2941 caactggcca tattcggagc aatttgaagc caatttccat cgcctggcga tcgctccatt 3001 cttggctata tgtttttcac cgttcccggg gccattttca aagactcgtc ggtaagataa 3061 gattgtgtca ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga 3121 cgtcggcgag tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg 3181 aattttctac aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga 3241 caaagattgt atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca 3301 atgatgatac tgatgatatt ttaagatgat gccagaccac aggctgattt ctgcgtcttt 3361 tgccgaacgc agtgcatgtg cggttgttgt tttttggaat agtttcaatt ttcggactgt 3421 ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga 3481 tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg 3541 aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg acgttggagc 3601 cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt 3661 tttttctttt tatctcgctc tctctagcgc catctcgtac gcatgctcaa cgcaccgcat 3721 gttgccgtgt cctttatgcg tcattttggc tcgaaatagg caattattta aacaaagatt 3781 agtcaacgaa aacgctaaaa taaataagtc tacaatatgg ttacttattg ccatgtgtgt 3841 gcagccaacg atagcaacaa aagcaacaac acagtggctt tccctctttc actttttgtt 3901 tgcaagcgcg tgcgagcaag acggcacgac cggcaaacgc aattacgctg acaaagagca 3961 gacgaagttt tggccgaaaa acatcaaggc gcctgatacg aatgcatttg caataacaat 4021 tgcgatattt aatattgttt atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata 4081 aaacaaatta tttgaaagag aattaggaat cggacagctt atcgttacgg gctaacagca 4141 caccgagacg aaatagctta cctgacgtca cagcctctgg aagaactgcc gccaagcaga 4201 cgatgcagag gacgacacat agagtagcgg agtaggccag cgtagtacgc atgtgcttgt 4261 gtgtgaggcg tctctctctt cgtctcctgt ttgcgcaaac gcatagactg cactgagaaa 4321 atcgattacc tattttttat gaatgaatat ttgcactatt actattcaaa actattaaga 4381 tagcaatcac attcaatagc caaatactat accacctgag cgatgcaacg aaatgatcaa 4441 tttgagcaaa aatgctgcat atttaggacg gcatcattat agaaatgctt cttgctgtgt 4501 acttttctct cgtctggcag ctgtttcgcc gttattgtta aaaccggctt aagttaggtg 4561 tgttttctac gactagctag tgatgcccct actagaagat gtgtgttgca caaatgtccc 4621 tgaataacca atttgaagtg cagatagcag taaacgtaag ctaatatgaa tattatttaa 4681 ctgtaatgtt ttaatatcgc tggacattac taataaaccc actataaaca catgtacata 4741 tgtatgtttt ggcatacaat gagtagttgg ggaaaaaatg tgtaaaagca ccgtgaccat 4801 cacagcataa agataaccag ctgaagtatc gaatatgagt aacccccaaa ttgaatcaca 4861 tgccgcaact gataggaccc atggaagtac actcttcatg gcgatataca agacacacac 4921 aagcacgaac acccagttgc ggaggaaatt ctccgtaaat gaaaacccaa tcggcgaaca 4981 attcataccc atatatggta aaagttttga acgcgacttg agagcggaga gcattgcggc 5041 tgataaggtt ttagcgctaa gcgggcttta taaaacgggc tgcgggacca gttttcatat 5101 cactaccgtt tgagttcttg tgctgtgtgg atactcctcc cgacacaaag ccgctccatc 5161 agccagcagt cgtctaatcc agagaccccg gatccgatat cactagttct agaggtaccg 5221 agctcgcggc cgcgttaacc gatccgtcga ccatgaagat caagatcatt gccccgccag 5281 agcgcaagta ctctgtctgg atcggtggct ccatcctggc ttcgctgtcc accttccagc 5341 agatgtggat ctccaagcag gagtacgacg agtccggccc ctccattgtg caccgcaagt 5401 gcttctaaga aggatcgctt gtctgggcaa gaggatcagg atcgggatgg tcttgattct 5461 gctggaggag gaggaggaga agtcgaggaa gcagcagcga aagtgcaagt gcgagtggtg 5521 gaagtttgga gtgcagcaca acaaaatcaa caacaacacc aactacaaga tgaaaagagc 5581 ggaaccacct gcacaccatc atcactatca tcatcgtttt gggcgcatgt tgtgtggttc 5641 cagcgtatta atataattaa tttattccac atgagatatg atatgatata ctatgtattt 5701 tttgtttttt ttttatttgt aaacctttaa tataacaaga actacaaaaa agtgaaaatg 5761 agcgaaaatg catattctgc cattccacac acacaccaac aacacccaac acacgcacac 5821 ccacaagctt acacacacac acattcgcgg catgacaagg acatcaagat aaagaagaac 5881 ttaaagaaga tatttcccaa agcgcaaaaa gaacacacac acattgcaaa acacaaacaa 5941 cacactagcg ttttgtacaa ttcgtcagca accttatgta ttatttttta attatgatgt 6001 aattataaac aaagtgaaac aaaaatatga aaacaaaaag gaaaatcaaa tctgtcttct 6061 ctttctcccg ctctcctcgc tctctgctgc taacctcgcc ctctcctctc tcatcttttt 6121 gtctgtctct cttcacattt ttgccggccg gcaaaataat aacccacaca cactcacact 6181 tggctgcagt ttcgcgtgcg atattcacac acattcaagc atacatacat atgtattttt 6241 tttttatttg tacacttttc taattgcatg cgtatcgatt gataagttta cgctgaaaat 6301 gttaattaaa atgtgaaaat gcaactgaaa aactgatgaa atgaaacaac aacaagcgaa 6361 caagtcgacc tgcaggcatg caagctt //pPacPL"Times New Roman^CM13_forward20_primer"Times New Roman^C #M13_pUC_fwd_primer"Times New Roman^CɆ lacZ_a"Times New Roman(^\r pGEX_3_primer"Times New Roman^C- AmpR_promoter"Times New Roman^CW Orf frame 2Times New Roman^CɆW AmpicillinTimes New Roman^N pBR322_origin"Times New Roman^C  lac_promoter"Times New Roman^C / M13_pUC_rev_primer"Times New Roman^CɆ. @ M13_reverse_primer"Times New Roman^  Orf frame 2"Times New Roman^C* AC5_primer"Times New Roman ^EcoRI"Times New RomanF "0:: ^BglII"Times New Roman &F ^SmaI"Times New Roman   1 ^NruI"Times New Roman*    ^BclI"Times New RomanR hovo} ^AflII"Times New Roman QY^We ^NcoI"Times New Roman   ^`BamHI(5190), EcoRV(5198), SpeI(5202), XbaI(5208), KpnI(5218), SacI(5224), NotI(5227), HpaI(5236)"Times New Romanb  ^test"Times New Roman cPCh, ^test"Times New Roman  @+)cxd