Sequence of pPacPL

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Sequence of pPacPL

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Sequence of pPacPL: 6387 BP; 1795 A; 1484 C; 1437 G; 1666 T; 5 other;

.......... .......... .......... .......... .......... ..........

1 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 60

1 ccgtgaccgg cagcaaaatg ttgcagcact gacccttttg ggaccgcaat gggttgaatt 60

. M13_forward20_primer M13_pUC_fwd_primer lacZ_a . ..........

.......... .......... .......... .......... .......... ..........

61 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 120

61 agcggaacgt cgtgtagggg gaaagcggtc gaccgcatta tcgcttctcc gggcgtggct 120

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct 180

121 agcgggaagg gttgtcaacg cgtcggactt accgcttacc gcggactacg ccataaaaga 180

.......... lacZ_a .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc 240

181 ggaatgcgta gacacgccat aaagtgtggc gtataccacg tgagagtcat gttagacgag 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg 300

241 actacggcgt atcaattcgg tcggggctgt gggcggttgt gggcgactgc gcgggactgc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 360

301 ccgaacagac gagggccgta ggcgaatgtc tgttcgacac tggcagaggc cctcgacgta 360

.......... .......... .......... .......... ........ pGEX_3_primer

.......... .......... .......... .......... .......... ..........

361 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg 420

361 cacagtctcc aaaagtggca gtagtggctt tgcgcgctct gctttcccgg agcactatgc 420

pGEX_3_primer ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt 480

421 ggataaaaat atccaattac agtactatta ttaccaaaga atctgcagtc caccgtgaaa 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 540

481 agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa gtttatacat 540

.......... .......... .......... .......... ........ AmpR_promoter

.......... .......... .......... .......... .......... ..........

541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 600

541 aggcgagtac tctgttattg ggactattta cgaagttatt ataacttttt ccttctcata 600

AmpR_promoter ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 660

601 ctcataagtt gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa cggaaggaca 660

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 720

661 aaaacgagtg ggtctttgcg accactttca ttttctacga cttctagtca acccacgtgc 720

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 780

721 tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa aagcggggct 780

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 840

781 tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc ataatagggc 840

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 900

841 ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg ataagagtct tactgaacca 900

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 960

901 actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt ctcttaatac 960

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1020

961 gtcacgacgg tattggtact cactattgtg acgccggttg aatgaagact gttgctagcc 1020

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1080

1021 tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt gagcggaact 1080

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1140

1081 agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt ggtgctacgg 1140

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1200

1141 acatcgttac cgttgttgca acgcgtttga taattgaccg cttgatgaat gagatcgaag 1200

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1260

1201 ggccgttgtt aattatctga cctacctccg cctatttcaa cgtcctggtg aagacgcgag 1260

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1320

1261 ccgggaaggc cgaccgacca aataacgact atttagacct cggccactcg cacccagagc 1320

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1380

1321 gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc aatagatgtg 1380

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1440

1381 ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct atccacggag 1440

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1500

1441 tgactaattc gtaaccattg acagtctggt tcaaatgagt atatatgaaa tctaactaaa 1500

Orf frame 2 Ampicillin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1560

1501 ttttgaagta aaaattaaat tttcctagat ccacttctag gaaaaactat tagagtactg 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1620

1561 gttttaggga attgcactca aaagcaaggt gactcgcagt ctggggcatc ttttctagtt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1680

1621 tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt gtttttttgg 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1740

1681 tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa aaggcttcca 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1800

1741 ttgaccgaag tcgtctcgcg tctatggttt atgacaggaa gatcacatcg gcatcaatcc 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1860

1801 ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt aggacaatgg 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1920

1861 tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt ctgctatcaa 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1980

1921 tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg ggtcgaacct 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2040

1981 cgcttgctgg atgtggcttg actctatgga tgtcgcactc gatactcttt cgcggtgcga 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2100

2041 agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt gtcctctcgc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2160

2101 gtgctccctc gaaggtcccc ctttgcggac catagaaata tcaggacagc ccaaagcggt 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2220

2161 ggagactgaa ctcgcagcta aaaacactac gagcagtccc cccgcctcgg ataccttttt 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2280

2221 gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac gagtgtacaa 2280

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2340

2281 gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac tcactcgact 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2400

2341 atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 2460

2401 cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 2520

2461 gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattac actcaatcga 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 2580

2521 gtgagtaatc cgtggggtcc gaaatgtgaa atacgaaggc cgagcataca acacacctta 2580

.......... .......... ......... lac_promoter ......... ..........

.......... .......... .......... ......... EcoRI(2630) .........

2581 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcnnnnn 2640

2581 acactcgcct attgttaaag tgtgtccttt gtcgatactg gtactaatgc ttaagnnnnn 2640

...... M13_pUC_rev_primer .. M13_reverse_primer ...... ..........

.......... .......... .......... .......... .......... ..........

2641 taaaaaaaaa tcatgaatgg catcaactct gaatcaaatc tttgcagatg cacctacttc 2700

2641 attttttttt agtacttacc gtagttgaga cttagtttag aaacgtctac gtggatgaag 2700

.......... .......... .......... .......... .......... ..........

.......... ......... BglII(2728) ......... .......... ..........

2701 tcatttccac tgtcacatca tttttccaga tctcgctgcc tgttatgtgg cccacaaacc 2760

2701 agtaaaggtg acagtgtagt aaaaaggtct agagcgacgg acaatacacc gggtgtttgg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aagacacgtt ttatggccat taaagctggc tgatcgtcgc caaacaccaa atacataatg 2820

2761 ttctgtgcaa aataccggta atttcgaccg actagcagcg gtttgtggtt tatgtattac 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aatatgtaca cattcgagaa agaagcgatc aaagaagcgt cttcgggcgg agtaggagaa 2880

2821 ttatacatgt gtaagctctt tcttcgctag tttcttcgca gaagcccgcc tcatcctctt 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgcggaggag aaggagaacg agctgatcta gtatctctcc acaatccaat gccaactgac 2940

2881 acgcctcctc ttcctcttgc tcgactagat catagagagg tgttaggtta cggttgactg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 caactggcca tattcggagc aatttgaagc caatttccat cgcctggcga tcgctccatt 3000

2941 gttgaccggt ataagcctcg ttaaacttcg gttaaaggta gcggaccgct agcgaggtaa 3000

.......... .......... .......... .......... .......... ..........

.......... .......... SmaI(3027) .......... .......... ..........

3001 cttggctata tgtttttcac cgttcccggg gccattttca aagactcgtc ggtaagataa 3060

3001 gaaccgatat acaaaaagtg gcaagggccc cggtaaaagt ttctgagcag ccattctatt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NruI(3114)

3061 gattgtgtca ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga 3120

3061 ctaacacagt gagcgacaga gagaagtaaa cagcttctta cgactcctta aagcgctact 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgtcggcgag tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg 3180

3121 gcagccgctc ataaaacttc ttactcttat taaacataaa tatgctttta gtcaatcacc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aattttctac aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga 3240

3181 ttaaaagatg tttttgtaca atagatatct attaaaacaa cgttttatac aactgatact 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 caaagattgt atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca 3300

3241 gtttctaaca tacatatatg gaaattacat aagagtaaaa gaatacataa atattaccgt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 atgatgatac tgatgatatt ttaagatgat gccagaccac aggctgattt ctgcgtcttt 3360

3301 tactactatg actactataa aattctacta cggtctggtg tccgactaaa gacgcagaaa 3360

.......... .......... .......... ......... Orf frame 2 .........

.......... .......... .......... .......... .......... ..........

3361 tgccgaacgc agtgcatgtg cggttgttgt tttttggaat agtttcaatt ttcggactgt 3420

3361 acggcttgcg tcacgtacac gccaacaaca aaaaacctta tcaaagttaa aagcctgaca 3420

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga 3480

3421 ggcgaaacta aagtcaaaga accgaataag tttttcgttt catttcggtt ttttcgctct 3480

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg 3540

3481 accgttatgg tttacgccgt tttgccatca ccttcctttc cccacgcccc gtcgccttcc 3540

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg acgttggagc 3600

3541 ttcccacccc gccccgcacc gccccagaca ccgacccgcg ctgcagtggc tgcaacctcg 3600

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt 3660

3601 gtgaggaaac tggtacacac gcacacacat aataagcaca gagcggtgag cggccaacaa 3660

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tttttctttt tatctcgctc tctctagcgc catctcgtac gcatgctcaa cgcaccgcat 3720

3661 aaaaagaaaa atagagcgag agagatcgcg gtagagcatg cgtacgagtt gcgtggcgta 3720

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gttgccgtgt cctttatgcg tcattttggc tcgaaatagg caattattta aacaaagatt 3780

3721 caacggcaca ggaaatacgc agtaaaaccg agctttatcc gttaataaat ttgtttctaa 3780

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agtcaacgaa aacgctaaaa taaataagtc tacaatatgg ttacttattg ccatgtgtgt 3840

3781 tcagttgctt ttgcgatttt atttattcag atgttatacc aatgaataac ggtacacaca 3840

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcagccaacg atagcaacaa aagcaacaac acagtggctt tccctctttc actttttgtt 3900

3841 cgtcggttgc tatcgttgtt ttcgttgttg tgtcaccgaa agggagaaag tgaaaaacaa 3900

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgcaagcgcg tgcgagcaag acggcacgac cggcaaacgc aattacgctg acaaagagca 3960

3901 acgttcgcgc acgctcgttc tgccgtgctg gccgtttgcg ttaatgcgac tgtttctcgt 3960

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gacgaagttt tggccgaaaa acatcaaggc gcctgatacg aatgcatttg caataacaat 4020

3961 ctgcttcaaa accggctttt tgtagttccg cggactatgc ttacgtaaac gttattgtta 4020

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgcgatattt aatattgttt atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata 4080

4021 acgctataaa ttataacaaa tacttcgaca aactgaagtt ttgtgtgttt ttttttttat 4080

......... Orf frame 2 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aaacaaatta tttgaaagag aattaggaat cggacagctt atcgttacgg gctaacagca 4140

4081 tttgtttaat aaactttctc ttaatcctta gcctgtcgaa tagcaatgcc cgattgtcgt 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 caccgagacg aaatagctta cctgacgtca cagcctctgg aagaactgcc gccaagcaga 4200

4141 gtggctctgc tttatcgaat ggactgcagt gtcggagacc ttcttgacgg cggttcgtct 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgatgcagag gacgacacat agagtagcgg agtaggccag cgtagtacgc atgtgcttgt 4260

4201 gctacgtctc ctgctgtgta tctcatcgcc tcatccggtc gcatcatgcg tacacgaaca 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtgtgaggcg tctctctctt cgtctcctgt ttgcgcaaac gcatagactg cactgagaaa 4320

4261 cacactccgc agagagagaa gcagaggaca aacgcgtttg cgtatctgac gtgactcttt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 atcgattacc tattttttat gaatgaatat ttgcactatt actattcaaa actattaaga 4380

4321 tagctaatgg ataaaaaata cttacttata aacgtgataa tgataagttt tgataattct 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... BclI(4434)

4381 tagcaatcac attcaatagc caaatactat accacctgag cgatgcaacg aaatgatcaa 4440

4381 atcgttagtg taagttatcg gtttatgata tggtggactc gctacgttgc tttactagtt 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tttgagcaaa aatgctgcat atttaggacg gcatcattat agaaatgctt cttgctgtgt 4500

4441 aaactcgttt ttacgacgta taaatcctgc cgtagtaata tctttacgaa gaacgacaca 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... AflII(4548) .........

4501 acttttctct cgtctggcag ctgtttcgcc gttattgtta aaaccggctt aagttaggtg 4560

4501 tgaaaagaga gcagaccgtc gacaaagcgg caataacaat tttggccgaa ttcaatccac 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tgttttctac gactagctag tgatgcccct actagaagat gtgtgttgca caaatgtccc 4620

4561 acaaaagatg ctgatcgatc actacgggga tgatcttcta cacacaacgt gtttacaggg 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgaataacca atttgaagtg cagatagcag taaacgtaag ctaatatgaa tattatttaa 4680

4621 acttattggt taaacttcac gtctatcgtc atttgcattc gattatactt ataataaatt 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctgtaatgtt ttaatatcgc tggacattac taataaaccc actataaaca catgtacata 4740

4681 gacattacaa aattatagcg acctgtaatg attatttggg tgatatttgt gtacatgtat 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tgtatgtttt ggcatacaat gagtagttgg ggaaaaaatg tgtaaaagca ccgtgaccat 4800

4741 acatacaaaa ccgtatgtta ctcatcaacc ccttttttac acattttcgt ggcactggta 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cacagcataa agataaccag ctgaagtatc gaatatgagt aacccccaaa ttgaatcaca 4860

4801 gtgtcgtatt tctattggtc gacttcatag cttatactca ttgggggttt aacttagtgt 4860

.......... .......... .......... .......... .......... ..........

.......... NcoI(4879) .......... .......... .......... ..........

4861 tgccgcaact gataggaccc atggaagtac actcttcatg gcgatataca agacacacac 4920

4861 acggcgttga ctatcctggg taccttcatg tgagaagtac cgctatatgt tctgtgtgtg 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aagcacgaac acccagttgc ggaggaaatt ctccgtaaat gaaaacccaa tcggcgaaca 4980

4921 ttcgtgcttg tgggtcaacg cctcctttaa gaggcattta cttttgggtt agccgcttgt 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 attcataccc atatatggta aaagttttga acgcgacttg agagcggaga gcattgcggc 5040

4981 taagtatggg tatataccat tttcaaaact tgcgctgaac tctcgcctct cgtaacgccg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tgataaggtt ttagcgctaa gcgggcttta taaaacgggc tgcgggacca gttttcatat 5100

5041 actattccaa aatcgcgatt cgcccgaaat attttgcccg acgccctggt caaaagtata 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cactaccgtt tgagttcttg tgctgtgtgg atactcctcc cgacacaaag ccgctccatc 5160

5101 gtgatggcaa actcaagaac acgacacacc tatgaggagg gctgtgtttc ggcgaggtag 5160

.......... .......... .......... .......... .......... AC5_primer

.......... ...... BamHI(5190) EcoRV(5198),SpeI(5202) XbaI(5208)

5161 agccagcagt cgtctaatcc agagaccccg gatccgatat cactagttct agaggtaccg 5220

5161 tcggtcgtca gcagattagg tctctggggc ctaggctata gtgatcaaga tctccatggc 5220

.......... .......... .......... .......... .......... ..........

SacI(5224),NotI(5227) HpaI(5236) ........ .......... ..........

5221 agctcgcggc cgcgttaacc gatccgtcga ccatgaagat caagatcatt gccccgccag 5280

5221 tcgagcgccg gcgcaattgg ctaggcagct ggtacttcta gttctagtaa cggggcggtc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 agcgcaagta ctctgtctgg atcggtggct ccatcctggc ttcgctgtcc accttccagc 5340

5281 tcgcgttcat gagacagacc tagccaccga ggtaggaccg aagcgacagg tggaaggtcg 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agatgtggat ctccaagcag gagtacgacg agtccggccc ctccattgtg caccgcaagt 5400

5341 tctacaccta gaggttcgtc ctcatgctgc tcaggccggg gaggtaacac gtggcgttca 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gcttctaaga aggatcgctt gtctgggcaa gaggatcagg atcgggatgg tcttgattct 5460

5401 cgaagattct tcctagcgaa cagacccgtt ctcctagtcc tagccctacc agaactaaga 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gctggaggag gaggaggaga agtcgaggaa gcagcagcga aagtgcaagt gcgagtggtg 5520

5461 cgacctcctc ctcctcctct tcagctcctt cgtcgtcgct ttcacgttca cgctcaccac 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gaagtttgga gtgcagcaca acaaaatcaa caacaacacc aactacaaga tgaaaagagc 5580

5521 cttcaaacct cacgtcgtgt tgttttagtt gttgttgtgg ttgatgttct acttttctcg 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ggaaccacct gcacaccatc atcactatca tcatcgtttt gggcgcatgt tgtgtggttc 5640

5581 ccttggtgga cgtgtggtag tagtgatagt agtagcaaaa cccgcgtaca acacaccaag 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cagcgtatta atataattaa tttattccac atgagatatg atatgatata ctatgtattt 5700

5641 gtcgcataat tatattaatt aaataaggtg tactctatac tatactatat gatacataaa 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tttgtttttt ttttatttgt aaacctttaa tataacaaga actacaaaaa agtgaaaatg 5760

5701 aaacaaaaaa aaaataaaca tttggaaatt atattgttct tgatgttttt tcacttttac 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 agcgaaaatg catattctgc cattccacac acacaccaac aacacccaac acacgcacac 5820

5761 tcgcttttac gtataagacg gtaaggtgtg tgtgtggttg ttgtgggttg tgtgcgtgtg 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ccacaagctt acacacacac acattcgcgg catgacaagg acatcaagat aaagaagaac 5880

5821 ggtgttcgaa tgtgtgtgtg tgtaagcgcc gtactgttcc tgtagttcta tttcttcttg 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ttaaagaaga tatttcccaa agcgcaaaaa gaacacacac acattgcaaa acacaaacaa 5940

5881 aatttcttct ataaagggtt tcgcgttttt cttgtgtgtg tgtaacgttt tgtgtttgtt 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 cacactagcg ttttgtacaa ttcgtcagca accttatgta ttatttttta attatgatgt 6000

5941 gtgtgatcgc aaaacatgtt aagcagtcgt tggaatacat aataaaaaat taatactaca 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aattataaac aaagtgaaac aaaaatatga aaacaaaaag gaaaatcaaa tctgtcttct 6060

6001 ttaatatttg tttcactttg tttttatact tttgtttttc cttttagttt agacagaaga 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ctttctcccg ctctcctcgc tctctgctgc taacctcgcc ctctcctctc tcatcttttt 6120

6061 gaaagagggc gagaggagcg agagacgacg attggagcgg gagaggagag agtagaaaaa 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gtctgtctct cttcacattt ttgccggccg gcaaaataat aacccacaca cactcacact 6180

6121 cagacagaga gaagtgtaaa aacggccggc cgttttatta ttgggtgtgt gtgagtgtga 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tggctgcagt ttcgcgtgcg atattcacac acattcaagc atacatacat atgtattttt 6240

6181 accgacgtca aagcgcacgc tataagtgtg tgtaagttcg tatgtatgta tacataaaaa 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tttttatttg tacacttttc taattgcatg cgtatcgatt gataagttta cgctgaaaat 6300

6241 aaaaataaac atgtgaaaag attaacgtac gcatagctaa ctattcaaat gcgactttta 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gttaattaaa atgtgaaaat gcaactgaaa aactgatgaa atgaaacaac aacaagcgaa 6360

6301 caattaattt tacactttta cgttgacttt ttgactactt tactttgttg ttgttcgctt 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 caagtcgacc tgcaggcatg caagctt... .......... .......... .......... 6387

6361 gttcagctgg acgtccgtac gttcgaa... .......... .......... .......... 6387

.......... .......... .......... .......... .......... ..........