Sequence of pQLinkHD

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Sequence of pQLinkHD

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Sequence of pQLinkHD : 7062 BP; 1836 A; 1714 C; 1640 G; 1872 T; 0 other;

XhoI(1) .......... .......... .......... .......... ..........

1 ctcgagctta attaacaaca ccatttgtcg agaaatcata aaaaatttat ttgctttgtg 60

1 gagctcgaat taattgttgt ggtaaacagc tctttagtat tttttaaata aacgaaacac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 agcggataac aattataata gattcaattg tgagcggata acaatttcac acagaattca 120

61 tcgcctattg ttaatattat ctaagttaac actcgcctat tgttaaagtg tgtcttaagt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttaaagagga gaaattaact atgaaacatc accatcacca tcaccatggt gctgcaggta 180

121 aatttctcct ctttaattga tactttgtag tggtagtggt agtggtacca cgacgtccat 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa 240

181 gttcaaacat gttttttcga cttgctcttt gcattttact atatttatag ttatataatt 240

.......... .......... attR1 .......... attR2 ..........

.......... .......... .......... .......... .......... ..........

241 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac 300

241 taatctaaaa cgtatttttt gtctgatgta ttatgacatt ttgtgttgta taggtcagtg 300

.......... ......... attR1 attR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tatgaatcaa ctacttagat ggtattagtg acctgtagtc gaccgacagc cttccaaatg 360

301 atacttagtt gatgaatcta ccataatcac tggacatcag ctggctgtcg gaaggtttac 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ttcttcgggt gatgctgcca acttagtcga ccgacagcct tccaaatgtt cttctcaaac 420

361 aagaagccca ctacgacggt tgaatcagct ggctgtcgga aggtttacaa gaagagtttg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggaatcgtcg tatccagcct actcgctatt gtcctcaatg ccgtattaaa tcataaaaag 480

421 ccttagcagc ataggtcgga tgagcgataa caggagttac ggcataattt agtatttttc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aaataagaaa aagaggtgcg agcctctttt ttgtgtgaca aaataaaaac atctacctat 540

481 tttattcttt ttctccacgc tcggagaaaa aacacactgt tttatttttg tagatggata 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tcatatacgc tagtgtcata gtcctgaaaa tcatctgcat caagaacaat ttcacaactc 600

541 agtatatgcg atcacagtat caggactttt agtagacgta gttcttgtta aagtgttgag 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ttatactttt ctcttacaag tcgttcggct tcatctggat tttcagcctc tatacttact 660

601 aatatgaaaa gagaatgttc agcaagccga agtagaccta aaagtcggag atatgaatga 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 aaacgtgata aagtttctgt aatttctact gtatcgacct gcagactggc tgtgtataag 720

661 tttgcactat ttcaaagaca ttaaagatga catagctgga cgtctgaccg acacatattc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc 780

721 cctcggactg taaatataag gggtcttgta gtccaattac cgcaaaaact acagtaaaag 780

.......... .......... .......... ccdB .......... ..........

.......... .......... .......... .......... .......... ..........

781 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc 840

781 cgccaccgac tctagtcggt gaagaagggg ctattgcctc tggccgtgtg accggtatag 840

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga 900

841 ccaccagtag tacgcggtcg aaagtagggg ctatacgtgg tggcccattt caagtgccct 900

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... SmaI(954)

901 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt 960

901 ctgaaataga ctgtcgtctg cacgtgaccg gtccccctag tggtaggcag cgggcccgca 960

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt 1020

961 cagttattat agtgagacat gtaggtgttt gtctgctatt gccgagagag aaaatatcca 1020

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt 1080

1021 catttggaat ttgacgtaaa gtggtcgggg acaagagcag tcgttttctc ggcaagtaaa 1080

ccdB .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac 1140

1081 gttatttggc ccgctggagt cggtagggaa ggactaaaag gcgaaaggtc gcaagccgtg 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat 1200

1141 cgtctgctgc ccgaagtaag acgtaccaac acgaatggtc tggcctctat aactgtagta 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat 1260

1201 tatacggaac tcgttgacta tcgacagcga cagttgacag tgacattatg cgacgaagta 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa 1320

1261 tcgtatggag aaaaactgta tgaagcccat atgtatagtc atatataaga atatggcgtt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... BamHI(1368) .........

1321 aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga tccacgcggc 1380

1321 tttagtcgcg cgtttatgcg tatgacaata gaccgaaaat cattcggcct aggtgcgccg 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtttacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg 1440

1381 caaatgcggg gcgggacggt gagtagcgtc atgacaacat taagtaattc gtaagacggc 1440

.......... .......... ........ ORF3 CAT/CamR ........ ..........

.......... .......... .......... .......... .......... ..........

1441 acatggaagc catcacagac ggcatgatga acctgaatcg ccagcggcat cagcaccttg 1500

1441 tgtaccttcg gtagtgtctg ccgtactact tggacttagc ggtcgccgta gtcgtggaac 1500

.......... ........ ORF3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg 1560

1501 agcggaacgc atattataaa cgggtaccac ttttgccccc gcttcttcaa caggtataac 1560

.......... ........ ORF3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata 1620

1561 cggtgcaaat ttagttttga ccactttgag tgggtcccta accgactctg ctttttgtat 1620

.......... ........ ORF3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc 1680

1621 aagagttatt tgggaaatcc ctttatccgg tccaaaagtg gcattgtgcg gtgtagaacg 1680

.......... ........ ORF3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac 1740

1681 cttatataca catctttgac ggcctttagc agcaccataa gtgaggtctc gctacttttg 1740

.......... ........ ORF3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc 1800

1741 caaagtcaaa cgagtacctt ttgccacatt gttcccactt gtgatagggt atagtggtcg 1800

.......... ........ ORF3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg 1860

1801 agtggcagaa agtaacggta tgccttaagg cctactcgta agtagtccgc ccgttcttac 1860

.......... ........ ORF3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta 1920

1861 acttatttcc ggcctatttt gaacacgaat aaaaagaaat gccagaaatt tttccggcat 1920

.......... ........ ORF3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa 1980

1921 tataggtcga cttgccagac caatatccat gtaactcgtt gactgacttt acggagtttt 1980

.......... ........ ORF3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc 2040

1981 acaagaaatg ctacggtaac cctatatagt tgccaccata taggtcacta aaaaaagagg 2040

.......... ........ ORF3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat 2100

2041 taaaatcgaa ggaatcgagg acttttagag ctattgagtt ttttatgcgg gccatcacta 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc 2160

2101 gaataaagta ataccacttt caaccttgga gaatgcacgg ctagttgcag agtaaaagcg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg 2220

2161 gttttcaacc gggtcccgaa gggccatagt tgtccctgtg gtcctaaata aataagacgc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac 2280

2221 ttcactagaa ggcagtgtcc ataaataagc cgcgtttcac gcagcccact acgacggttg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ttagtcgact acaggtcact aataccatct aagtagttga ttcatagtga ctggatatgt 2340

2281 aatcagctga tgtccagtga ttatggtaga ttcatcaact aagtatcact gacctataca 2340

.......... .......... .......... .......... ......... attR2 attR1

.......... .......... .......... .......... .......... ..........

2341 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata 2400

2341 acacaaaatg tcataataca tcagacaaaa aatacgtttt agattaaatt atataactat 2400

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... ........ HindIII(2448) ........

2401 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtaag cttaattagc 2460

2401 aaatatagta aaatgcaaag agcaagtcga aagaacatgt ttcaccattc gaattaatcg 2460

......... attR2 attR1 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgagcttgga ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt 2520

2461 actcgaacct gaggacaact atctaggtca ttactggagt cttgaggtag acctaaacaa 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagta acaacaccat 2580

2521 gtcttgcgag ccaacggcgg cccgcaaaaa ataaccactc ttaggttcat tgttgtggta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NheI(2618) .......... ..........

2581 ttaaatggag tggttacaaa tggagtggtt aattaaggct agcttggcga gattttcagg 2640

2581 aatttacctc accaatgttt acctcaccaa ttaattccga tcgaaccgct ctaaaagtcc 2640

.......... .......... .......... ORF2 .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca 2700

2641 tcgattcctt cgattttacc tcttttttta gtgacctata tggtggcaac tatatagggt 2700

.......... .......... ORF2 CAT/CamR .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca 2760

2701 taccgtagca tttcttgtaa aactccgtaa agtcagtcaa cgagttacat ggatattggt 2760

.......... ........ ORF2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt 2820

2761 ctggcaagtc gacctataat gccggaaaaa tttctggcat ttctttttat tcgtgttcaa 2820

.......... ........ ORF2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat 2880

2821 aataggccgg aaataagtgt aagaacgggc ggactactta cgagtaggcc ttaaagcata 2880

.......... ........ ORF2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt 2940

2881 ccgttacttt ctgccactcg accactatac cctatcacaa gtgggaacaa tgtggcaaaa 2940

.......... ........ ORF2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca 3000

2941 ggtactcgtt tgactttgca aaagtagcga gacctcactt atggtgctgc taaaggccgt 3000

.......... ........ ORF2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc 3060

3001 caaagatgtg tatataagcg ttctacaccg cacaatgcca cttttggacc ggataaaggg 3060

.......... ........ ORF2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag 3120

3061 atttcccaaa taactcttat acaaaaagca gagtcggtta gggacccact caaagtggtc 3120

.......... ........ ORF2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa 3180

3121 aaaactaaat ttgcaccggt tatacctgtt gaagaagcgg gggcaaaagt ggtacccgtt 3180

.......... ........ ORF2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt 3240

3181 tataatatgc gttccgctgt tccacgacta cggcgaccgc taagtccaag tagtacggca 3240

.......... ........ ORF2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg 3300

3241 aacactaccg aaggtacagc cgtcttacga attacttaat gttgtcatga cgctactcac 3300

.......... ........ ORF2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggggtaat 3360

3301 cgtcccgccc cgcattaaaa aaattccgtc aataaccacg ggaatttgcg gaccccatta 3360

ORF2 CAT/CamR ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gactctctag cttgaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 3420

3361 ctgagagatc gaactccgta gtttattttg ctttccgagt cagctttctg acccggaaag 3420

.......... .......... .......... ...... rrnB_T1_terminator ......

.......... .......... .......... .......... .......... ..........

3421 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccctctagat 3480

3421 caaaatagac aacaaacagc cacttgcgag aggactcatc ctgtttaggc gggagatcta 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tacgtgcagt cgatgataag ctgtcaaaca tgagaattgt gcctaatgag tgagctaact 3540

3481 atgcacgtca gctactattc gacagtttgt actcttaaca cggattactc actcgattga 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 3600

3541 atgtaattaa cgcaacgcga gtgacgggcg aaaggtcagc cctttggaca gcacggtcga 3600

.......... .......... ....... Orf frame 2 lacI ....... ..........

.......... .......... .......... .......... NarI(3649) ..........

3601 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg 3660

3601 cgtaattact tagccggttg cgcgcccctc tccgccaaac gcataacccg cggtcccacc 3660

.......... ....... Orf frame 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag 3720

3661 aaaaagaaaa gtggtcactc tgcccgttgt cgactaacgg gaagtggcgg accgggactc 3720

.......... ....... Orf frame 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg 3780

3721 tctcaacgtc gttcgccagg tgcgaccaaa cggggtcgtc cgcttttagg acaaactacc 3780

.......... ....... Orf frame 2 lacI ....... .......... ..........

HpaI(3785) .......... .......... .......... .......... ..........

3781 tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga 3840

3781 accaattgcc gccctatatt gtactcgaca gaagccatag cagcataggg tgatggctct 3840

.......... ....... Orf frame 2 lacI ....... .......... ..........

EcoRV(3841) ........ .......... .......... .......... ..........

3841 tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct 3900

3841 ataggcgtgg ttgcgcgtcg ggcctgagcc attaccgcgc gtaacgcggg tcgcggtaga 3900

.......... ....... Orf frame 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt 3960

3901 ctagcaaccg ttggtcgtag cgtcaccctt gctacgggag taagtcgtaa acgtaccaaa 3960

.......... ....... Orf frame 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat 4020

3961 caacttttgg cctgtaccgt gaggtcagcg gaagggcaag gcgatagccg acttaaacta 4020

.......... ....... Orf frame 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg 4080

4021 acgctcactc tataaatacg gtcggtcggt ctgcgtctgc gcggctctgt cttgaattac 4080

.......... ....... Orf frame 2 lacI ....... .......... ..........

ApaI(4084) .......... .......... .......... .......... ..........

4081 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc 4140

4081 ccgggcgatt gtcgcgctaa acgaccactg ggttacgctg gtctacgagg tgcgggtcag 4140

.......... ....... Orf frame 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa 4200

4141 cgcatggcag aagtaccctc ttttattatg acaactaccc acagaccagt ctctgtagtt 4200

.......... ....... Orf frame 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca 4260

4201 ctttattgcg gccttgtaat cacgtccgtc gaaggtgtcg ttaccgtagg accagtaggt 4260

.......... ....... Orf frame 2 lacI ....... .......... ..........

.......... BclI(4273) .......... .......... .......... ..........

4261 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt 4320

4261 cgcctatcaa ttactagtcg ggtgactgcg caacgcgctc ttctaacacg tggcggcgaa 4320

.......... ....... Orf frame 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat 4380

4321 atgtccgaag ctgcggcgaa gcaagatggt agctgtggtg gtgcgaccgt gggtcaacta 4380

.......... ....... Orf frame 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg 4440

4381 gccgcgctct aaattagcgg cgctgttaaa cgctgccgcg cacgtcccgg tctgacctcc 4440

.......... ....... Orf frame 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa 4500

4441 accgttgcgg ttagtcgttg ctgacaaacg ggcggtcaac aacacggtgc gccaaccctt 4500

.......... ....... Orf frame 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc 4560

4501 acattaagtc gaggcggtag cggcgaaggt gaaaaagggc gcaaaagcgt ctttgcaccg 4560

Orf frame 2 ........ lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat 4620

4561 accggaccaa gtggtgcgcc ctttgccaga ctattctctg tggccgtatg agacgctgta 4620

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc 4680

4621 gcatattgca atgaccaaag tgtaagtggt gggacttaac tgagagaagg cccgcgatag 4680

.......... lacI .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc ggaatttcgg gcagcgttgg 4740

4681 tacggtatgg cgctttccaa aacgtggtaa gctaccacag ccttaaagcc cgtcgcaacc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gtcctggcca cgggtgcgca tgatctagag ctgcctcgcg cgtttcggtg atgacggtga 4800

4741 caggaccggt gcccacgcgt actagatctc gacggagcgc gcaaagccac tactgccact 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 4860

4801 tttggagact gtgtacgtcg agggcctctg ccagtgtcga acagacattc gcctacggcc 4860

........ pGEX_3_primer ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat 4920

4861 ctcgtctgtt cgggcagtcc cgcgcagtcg cccacaaccg cccacagccc cgcgtcggta 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag 4980

4921 ctgggtcagt gcatcgctat cgcctcacat atgaccgaat tgatacgccg tagtctcgtc 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 attgtactga gagtgcacca catgcggtgt gaaataccgc acagatgcgt aaggagaaaa 5040

4981 taacatgact ctcacgtggt gtacgccaca ctttatggcg tgtctacgca ttcctctttt 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 5100

5041 atggcgtagt ccgcgagaag gcgaaggagc gagtgactga gcgacgcgag ccagcaagcc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 5160

5101 gacgccgctc gccatagtcg agtgagtttc cgccattatg ccaataggtg tcttagtccc 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 5220

5161 ctattgcgtc ctttcttgta cactcgtttt ccggtcgttt tccggtcctt ggcatttttc 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 5280

5221 cggcgcaacg accgcaaaaa ggtatccgag gcggggggac tgctcgtagt gtttttagct 5280

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

5281 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 5340

5281 gcgagttcag tctccaccgc tttgggctgt cctgatattt ctatggtccg caaaggggga 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 5400

5341 ccttcgaggg agcacgcgag aggacaaggc tgggacggcg aatggcctat ggacaggcgg 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 5460

5401 aaagagggaa gcccttcgca ccgcgaaaga gtatcgagtg cgacatccat agagtcaagc 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 5520

5461 cacatccagc aagcgaggtt cgacccgaca cacgtgcttg gggggcaagt cgggctggcg 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 5580

5521 acgcggaata ggccattgat agcagaactc aggttgggcc attctgtgct gaatagcggt 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 5640

5581 gaccgtcgtc ggtgaccatt gtcctaatcg tctcgctcca tacatccgcc acgatgtctc 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 5700

5641 aagaacttca ccaccggatt gatgccgatg tgatcttcct gtcataaacc atagacgcga 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 5760

5701 gacgacttcg gtcaatggaa gcctttttct caaccatcga gaactaggcc gtttgtttgg 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 5820

5761 tggcgaccat cgccaccaaa aaaacaaacg ttcgtcgtct aatgcgcgtc tttttttcct 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 5880

5821 agagttcttc taggaaacta gaaaagatgc cccagactgc gagtcacctt gcttttgagt 5880

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 5940

5881 gcaattccct aaaaccagta ctctaatagt ttttcctaga agtggatcta ggaaaattta 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 6000

5941 atttttactt caaaatttag ttagatttca tatatactca tttgaaccag actgtcaatg 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 6060

6001 gttacgaatt agtcactccg tggatagagt cgctagacag ataaagcaag taggtatcaa 6060

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 6120

6061 cggactgagg ggcagcacat ctattgatgc tatgccctcc cgaatggtag accggggtca 6120

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 6180

6121 cgacgttact atggcgctct gggtgcgagt ggccgaggtc taaatagtcg ttatttggtc 6180

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 6240

6181 ggtcggcctt cccggctcgc gtcttcacca ggacgttgaa ataggcggag gtaggtcaga 6240

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 6300

6241 taattaacaa cggcccttcg atctcattca tcaagcggtc aattatcaaa cgcgttgcaa 6300

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 6360

6301 caacggtaac gatgtccgta gcaccacagt gcgagcagca aaccataccg aagtaagtcg 6360

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 6420

6361 aggccaaggg ttgctagttc cgctcaatgt actagggggt acaacacgtt ttttcgccaa 6420

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 6480

6421 tcgaggaagc caggaggcta gcaacagtct tcattcaacc ggcgtcacaa tagtgagtac 6480

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 6540

6481 caataccgtc gtgacgtatt aagagaatga cagtacggta ggcattctac gaaaagacac 6540

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 6600

6541 tgaccactca tgagttggtt cagtaagact cttatcacat acgccgctgg ctcaacgaga 6600

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 6660

6601 acgggccgca gttatgccct attatggcgc ggtgtatcgt cttgaaattt tcacgagtag 6660

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 6720

6661 taaccttttg caagaagccc cgcttttgag agttcctaga atggcgacaa ctctaggtca 6720

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 6780

6721 agctacattg ggtgagcacg tgggttgact agaagtcgta gaaaatgaaa gtggtcgcaa 6780

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 6840

6781 agacccactc gtttttgtcc ttccgtttta cggcgttttt tcccttattc ccgctgtgcc 6840

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 6900

6841 tttacaactt atgagtatga gaaggaaaaa gttataataa cttcgtaaat agtcccaata 6900

Orf frame 3 Ampicillin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 6960

6901 acagagtact cgcctatgta taaacttaca taaatctttt tatttgttta tccccaaggc 6960

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... ......... AatII(6992) ......... ..........

6961 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 7020

6961 gcgtgtaaag gggcttttca cggtggactg cagattcttt ggtaataata gtactgtaat 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 acctataaaa ataggcgtat cacgaggccc tttcgtcttc ac........ .......... 7062

7021 tggatatttt tatccgcata gtgctccggg aaagcagaag tg........ .......... 7062

.......... .......... .......... .......... .......... ..........