Sequence of pQLinkH

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Sequence of pQLinkH

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Sequence of pQLinkH : 4901 BP; 1232 A; 1212 C; 1218 G; 1239 T; 0 other;

Xhol(1) .......... .......... .......... .......... ..........

1 ctcgagctta attaacaaca ccatttgtcg agaaatcata aaaaatttat ttgctttgtg 60

1 gagctcgaat taattgttgt ggtaaacagc tctttagtat tttttaaata aacgaaacac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 agcggataac aattataata gattcaattg tgagcggata acaatttcac acagaattca 120

61 tcgcctattg ttaatattat ctaagttaac actcgcctat tgttaaagtg tgtcttaagt 120

.......... .......... .......... .......... .......... ..........

EcoRI(121) .......... .......... .......... .......... ..........

121 ttaaagagga gaaattaact atgaaacatc accatcacca tcaccatggt gctgcaggta 180

121 aatttctcct ctttaattga tactttgtag tggtagtggt agtggtacca cgacgtccat 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 caagtttgta caaaaaagca ggcgagaatc tttattttca gggatccagt cttcgcatgc 240

181 gttcaaacat gttttttcgt ccgctcttag aaataaaagt ccctaggtca gaagcgtacg 240

.......... attB1 .......... .......... .......... ..........

EagI(250),NotI(250) .......... ......... HindIII(287) .........

241 atgaagacgc ggccgcctag gacccagctt tcttgtacaa agtggtaagc ttaattagct 300

241 tacttctgcg ccggcggatc ctgggtcgaa agaacatgtt tcaccattcg aattaatcga 300

.......... .......... .......... attB2 .......... ..........

.......... .......... .......... .......... .......... ..........

301 gagcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc 360

301 ctcgaacctg aggacaacta tctaggtcat tactggagtc ttgaggtaga cctaaacaag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagtaa caacaccatt 420

361 tcttgcgagc caacggcggc ccgcaaaaaa taaccactct taggttcatt gttgtggtaa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NheI(457) .......... ..........

421 taaatggagt ggttacaaat ggagtggtta attaaggcta gcttggcgag attttcagga 480

421 atttacctca ccaatgttta cctcaccaat taattccgat cgaaccgctc taaaagtcct 480

.......... .......... ......... Orf frame 1 ......... ..........

.......... .......... .......... .......... .......... ..........

481 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa 540

481 cgattccttc gattttacct ctttttttag tgacctatat ggtggcaact atatagggtt 540

.......... ......... Orf frame 1 CAT/CamR ......... ..........

.......... .......... .......... .......... .......... ..........

541 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag 600

541 accgtagcat ttcttgtaaa actccgtaaa gtcagtcaac gagttacatg gatattggtc 600

.......... ..... Orf frame 1 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt 660

601 tggcaagtcg acctataatg ccggaaaaat ttctggcatt tctttttatt cgtgttcaaa 660

.......... ..... Orf frame 1 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg 720

661 ataggccgga aataagtgta agaacgggcg gactacttac gagtaggcct taaagcatac 720

.......... ..... Orf frame 1 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc 780

721 cgttactttc tgccactcga ccactatacc ctatcacaag tgggaacaat gtggcaaaag 780

.......... ..... Orf frame 1 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

781 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag 840

781 gtactcgttt gactttgcaa aagtagcgag acctcactta tggtgctgct aaaggccgtc 840

.......... ..... Orf frame 1 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct 900

841 aaagatgtgt atataagcgt tctacaccgc acaatgccac ttttggaccg gataaaggga 900

.......... ..... Orf frame 1 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt 960

901 tttcccaaat aactcttata caaaaagcag agtcggttag ggacccactc aaagtggtca 960

.......... ..... Orf frame 1 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa 1020

961 aaactaaatt tgcaccggtt atacctgttg aagaagcggg ggcaaaagtg gtacccgttt 1020

.......... ..... Orf frame 1 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt 1080

1021 ataatatgcg ttccgctgtt ccacgactac ggcgaccgct aagtccaagt agtacggcaa 1080

.......... ..... Orf frame 1 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg 1140

1081 acactaccga aggtacagcc gtcttacgaa ttacttaatg ttgtcatgac gctactcacc 1140

.......... ..... Orf frame 1 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg 1200

1141 gtcccgcccc gcattaaaaa aattccgtca ataaccacgg gaatttgcgg accccattac 1200

Orf frame 1 CAT/CamR .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1260

1201 tgagagatcg aactccgtag tttattttgc tttccgagtc agctttctga cccggaaagc 1260

.......... .......... .......... ...... rrnB_T1_terminator ......

.......... .......... .......... .......... .......... ..........

1261 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cctctagatt 1320

1261 aaaatagaca acaaacagcc acttgcgaga ggactcatcc tgtttaggcg ggagatctaa 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acgtgcagtc gatgataagc tgtcaaacat gagaattgtg cctaatgagt gagctaactt 1380

1321 tgcacgtcag ctactattcg acagtttgta ctcttaacac ggattactca ctcgattgaa 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 1440

1381 tgtaattaac gcaacgcgag tgacgggcga aaggtcagcc ctttggacag cacggtcgac 1440

.......... .......... ....... Orf frame 1 lacI ....... ..........

.......... .......... .......... .......... NarI(1488) ..........

1441 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt 1500

1441 gtaattactt agccggttgc gcgcccctct ccgccaaacg cataacccgc ggtcccacca 1500

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga 1560

1501 aaaagaaaag tggtcactct gcccgttgtc gactaacggg aagtggcgga ccgggactct 1560

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt 1620

1561 ctcaacgtcg ttcgccaggt gcgaccaaac ggggtcgtcc gcttttagga caaactacca 1620

.......... ....... Orf frame 1 lacI ....... .......... ..........

HpaI(1624) .......... .......... .......... ......... EcoRV(1680)

1621 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat 1680

1621 ccaattgccg ccctatattg tactcgacag aagccatagc agcatagggt gatggctcta 1680

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg 1740

1681 taggcgtggt tgcgcgtcgg gcctgagcca ttaccgcgcg taacgcgggt cgcggtagac 1740

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg 1800

1741 tagcaaccgt tggtcgtagc gtcacccttg ctacgggagt aagtcgtaaa cgtaccaaac 1800

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt 1860

1801 aacttttggc ctgtaccgtg aggtcagcgg aagggcaagg cgatagccga cttaaactaa 1860

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg 1920

1861 cgctcactct ataaatacgg tcggtcggtc tgcgtctgcg cggctctgtc ttgaattacc 1920

.......... ....... Orf frame 1 lacI ....... .......... ..........

ApaI(1923) .......... .......... .......... .......... ..........

1921 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg 1980

1921 cgggcgattg tcgcgctaaa cgaccactgg gttacgctgg tctacgaggt gcgggtcagc 1980

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag 2040

1981 gcatggcaga agtaccctct tttattatga caactaccca cagaccagtc tctgtagttc 2040

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag 2100

2041 tttattgcgg ccttgtaatc acgtccgtcg aaggtgtcgt taccgtagga ccagtaggtc 2100

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... BclI(2112) .......... .......... .......... ..........

2101 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt 2160

2101 gcctatcaat tactagtcgg gtgactgcgc aacgcgctct tctaacacgt ggcggcgaaa 2160

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc 2220

2161 tgtccgaagc tgcggcgaag caagatggta gctgtggtgg tgcgaccgtg ggtcaactag 2220

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt 2280

2221 ccgcgctcta aattagcggc gctgttaaac gctgccgcgc acgtcccggt ctgacctcca 2280

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat 2340

2281 ccgttgcggt tagtcgttgc tgacaaacgg gcggtcaaca acacggtgcg ccaaccctta 2340

.......... ....... Orf frame 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct 2400

2341 cattaagtcg aggcggtagc ggcgaaggtg aaaaagggcg caaaagcgtc tttgcaccga 2400

Orf frame 1 ........ lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc 2460

2401 ccggaccaag tggtgcgccc tttgccagac tattctctgt ggccgtatga gacgctgtag 2460

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca 2520

2461 catattgcaa tgaccaaagt gtaagtggtg ggacttaact gagagaaggc ccgcgatagt 2520

.......... lacI .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgccataccg cgaaaggttt tgcaccattc gatggtgtcg gaatttcggg cagcgttggg 2580

2521 acggtatggc gctttccaaa acgtggtaag ctaccacagc cttaaagccc gtcgcaaccc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcctggccac gggtgcgcat gatctagagc tgcctcgcgc gtttcggtga tgacggtgaa 2640

2581 aggaccggtg cccacgcgta ctagatctcg acggagcgcg caaagccact actgccactt 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 2700

2641 ttggagactg tgtacgtcga gggcctctgc cagtgtcgaa cagacattcg cctacggccc 2700

........ pGEX_3_primer ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg 2760

2701 tcgtctgttc gggcagtccc gcgcagtcgc ccacaaccgc ccacagcccc gcgtcggtac 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga 2820

2761 tgggtcagtg catcgctatc gcctcacata tgaccgaatt gatacgccgt agtctcgtct 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttgtactgag agtgcaccac atgcggtgtg aaataccgca cagatgcgta aggagaaaat 2880

2821 aacatgactc tcacgtggtg tacgccacac tttatggcgt gtctacgcat tcctctttta 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 2940

2881 tggcgtagtc cgcgagaagg cgaaggagcg agtgactgag cgacgcgagc cagcaagccg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 3000

2941 acgccgctcg ccatagtcga gtgagtttcc gccattatgc caataggtgt cttagtcccc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 3060

3001 tattgcgtcc tttcttgtac actcgttttc cggtcgtttt ccggtccttg gcatttttcc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 3120

3061 ggcgcaacga ccgcaaaaag gtatccgagg cggggggact gctcgtagtg tttttagctg 3120

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3121 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 3180

3121 cgagttcagt ctccaccgct ttgggctgtc ctgatatttc tatggtccgc aaagggggac 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 3240

3181 cttcgaggga gcacgcgaga ggacaaggct gggacggcga atggcctatg gacaggcgga 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 3300

3241 aagagggaag cccttcgcac cgcgaaagag tatcgagtgc gacatccata gagtcaagcc 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 3360

3301 acatccagca agcgaggttc gacccgacac acgtgcttgg ggggcaagtc gggctggcga 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 3420

3361 cgcggaatag gccattgata gcagaactca ggttgggcca ttctgtgctg aatagcggtg 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 3480

3421 accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat acatccgcca cgatgtctca 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 3540

3481 agaacttcac caccggattg atgccgatgt gatcttcctg tcataaacca tagacgcgag 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 3600

3541 acgacttcgg tcaatggaag cctttttctc aaccatcgag aactaggccg tttgtttggt 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 3660

3601 ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct ttttttccta 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 3720

3661 gagttcttct aggaaactag aaaagatgcc ccagactgcg agtcaccttg cttttgagtg 3720

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 3780

3721 caattcccta aaaccagtac tctaatagtt tttcctagaa gtggatctag gaaaatttaa 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 3840

3781 tttttacttc aaaatttagt tagatttcat atatactcat ttgaaccaga ctgtcaatgg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 3900

3841 ttacgaatta gtcactccgt ggatagagtc gctagacaga taaagcaagt aggtatcaac 3900

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 3960

3901 ggactgaggg gcagcacatc tattgatgct atgccctccc gaatggtaga ccggggtcac 3960

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 4020

3961 gacgttacta tggcgctctg ggtgcgagtg gccgaggtct aaatagtcgt tatttggtcg 4020

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 4080

4021 gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa taggcggagg taggtcagat 4080

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 4140

4081 aattaacaac ggcccttcga tctcattcat caagcggtca attatcaaac gcgttgcaac 4140

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 4200

4141 aacggtaacg atgtccgtag caccacagtg cgagcagcaa accataccga agtaagtcga 4200

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 4260

4201 ggccaagggt tgctagttcc gctcaatgta ctagggggta caacacgttt tttcgccaat 4260

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 4320

4261 cgaggaagcc aggaggctag caacagtctt cattcaaccg gcgtcacaat agtgagtacc 4320

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 4380

4321 aataccgtcg tgacgtatta agagaatgac agtacggtag gcattctacg aaaagacact 4380

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 4440

4381 gaccactcat gagttggttc agtaagactc ttatcacata cgccgctggc tcaacgagaa 4440

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 4500

4441 cgggccgcag ttatgcccta ttatggcgcg gtgtatcgtc ttgaaatttt cacgagtagt 4500

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 4560

4501 aaccttttgc aagaagcccc gcttttgaga gttcctagaa tggcgacaac tctaggtcaa 4560

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 4620

4561 gctacattgg gtgagcacgt gggttgacta gaagtcgtag aaaatgaaag tggtcgcaaa 4620

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 4680

4621 gacccactcg tttttgtcct tccgttttac ggcgtttttt cccttattcc cgctgtgcct 4680

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 4740

4681 ttacaactta tgagtatgag aaggaaaaag ttataataac ttcgtaaata gtcccaataa 4740

Orf frame 2 Ampicillin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 4800

4741 cagagtactc gcctatgtat aaacttacat aaatcttttt atttgtttat ccccaaggcg 4800

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... ......... AatII(4831) ......... ..........

4801 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 4860

4801 cgtgtaaagg ggcttttcac ggtggactgc agattctttg gtaataatag tactgtaatt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cctataaaaa taggcgtatc acgaggccct ttcgtcttca c......... .......... 4901

4861 ggatattttt atccgcatag tgctccggga aagcagaagt g......... .......... 4901

.......... .......... .......... .......... .......... ..........