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    Plasmid map of pRK793

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    pRK793
    Gene/insert name: TEV protease, S219V mutant
    Alternative names: tobacco etch virus protease
    Insert size (bp): 750
    Species of gene(s): Other
    Relevant mutations/deletions: S219V mutation
    Fusion proteins or tags: His
    Terminal: N terminal on insert
    Fusion proteins or tags: polyarginine
    Terminal: C terminal on insert
    Vector backbone: pMal-C2 (Search Vector Database)
    Backbone manufacturer: New England Biolabs
    Type of vector: Bacterial expression
    Backbone size (bp): 6700
    Cloning site 5': SacI
    Site destroyed during cloning: No
    Cloning site 3': BamHI
    Site destroyed during cloning: No
    5' Sequencing primer: N/A (List of Sequencing Primers)
    3' Sequencing primer: M13-F20
    Bacteria resistance: Ampicillin for p793; Chloramphenicol for pRIL.
    High or low copy: Low Copy
    Grow in standard E. coli @ 37C: No
    Bacterial strain for growth and growth condition: Shifting the temperature from 37C to 30C during induction maximizes the yield of soluble TEV protease.
    If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: ATCC
    Plasmid Provided In: BL21(DE3)-RIL
    Principal Investigator: David S. Waugh
    Comments: Bacterial strain: E.coli BL21(DE3)-RIL (Stratagene).
    Article: Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Kapust RB et al. (Protein Eng. 2001 Dec . 14(12):993-1000. Pubmed)



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