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    Plasmid map of pRL-TK CXCR4 2x

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    pRL-TK CXCR4 2x
    Gene/insert name: 2 bulged bind sites for CXCR4 siRNA antisense
    Insert size (bp): 69
    Fusion proteins or tags: Rr-luc
    Terminal: N terminal on backbone
    Vector backbone: pRL-TK
    Backbone manufacturer: Promega
    Type of vector: Luciferase
    Backbone size (bp): 4045
    Cloning site 5': XbaI
    Site destroyed during cloning: No
    Cloning site 3': ApaI
    Site destroyed during cloning: No
    5' Sequencing primer: See map
    3' Sequencing primer: EBV-rev
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Principal Investigator: Phil Sharp
    Comments: The end of the ORF and the beginning of the 3' UTR is (with the TAA stop codon, followed by T, then the XbaI site TCTAGA): AAATGAACAA TAA T TCTAGA GCGGCCGCT.
    Article: siRNAs can function as miRNAs. Doench JG et al. (Genes Dev. 2003 Feb 15. 17(4):438-42. Pubmed)

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