Gene/insert name: 2 bulged bind sites for CXCR4 siRNA antisense
Insert size (bp): 69
Fusion proteins or tags: Rr-luc
Terminal: N terminal on backbone
Vector backbone: pRL-TK
Backbone manufacturer: Promega
Type of vector: Luciferase
Backbone size (bp): 4045
Cloning site 5': XbaI
Site destroyed during cloning: No
Cloning site 3': ApaI
Site destroyed during cloning: No
5' Sequencing primer: See map
3' Sequencing primer: EBV-rev
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Principal Investigator: Phil Sharp
Comments: The end of the ORF and the beginning of the 3' UTR is (with the TAA stop codon, followed by T, then the XbaI site TCTAGA): AAATGAACAA TAA T TCTAGA GCGGCCGCT.
Article: siRNAs can function as miRNAs. Doench JG et al. (Genes Dev. 2003 Feb 15. 17(4):438-42. Pubmed)